Molecular characterization of secaloindoline genes in introduced CIMMYT primary hexaploid triticale

In order to widen gene germplasm for kernel hardness in triticale, 60 synthetic hexaploid triticales were tested by the single kernel characterization system(SKCS) and secaloindoline alleles were identified by sequencing. Phenot^ing showed that frequencies of soft, mixed and hard genotypes were 43.3%, 48.3%, and 8.4%, respectively. Genotyping identified three known secaloindoline-a alleles and four known secaloindoline-b alleles. Three new Sina-Rl alleles were designated Sina-Rld, Sina-Rle and Sina-Rlf. Compared to Sina-Dlc, Sina-Rld showed four point mutations causing changes in four amino acids, Sina-Rle had one point mutation causing an alanine to glycine substitution, and Sina-Rlf possessed five point mutations but produced the same amino add sequence as Sina-Rld. Two new Sinb-Rl alleles were discovered and designated Sinb-Rle and Sinb-Rl/. Compared to Sinb-Rla, Sinb-Rle possessed a triplet-code insertion and four point mutations causing a cysteine insertion and two amino acid substitutions, and Sinb-Rl/possessed three point mutations causing a cysteine insertion and a change of arginine to glycine.Association of hardness index with secaloindoline alleles indicated t±iat SKCS of the Sina-Rld genotype was significantly lower than that of Sina-Rle, and Sinb-Rle was significantly lower than that of the Sinb-Rld genotype. A total of eight allelic combinations of secaloindoline genes were identified; Sma-Rld/Sinb-Rle and Sina-Rle/Sinb-Rld were relatively prevalent in the triticales surveyed. The results provide valuable information for further use of these germplasms in triticale breeding program due to the diverse polymorphism in secaloindoline genes.

Typical Correlation Analysis between Forage Type Triticale Production Performance and Different Pilot Ecological Factors

Domestic and foreign researches on triticale mainly focus on hereditary traits and excellent characteristics,but there are few reports on triticale yield and quality in the severe cold pastoral areas of Gansu and Qinghai.In this study,Gannong 2 triticale cultivars have been bred and planted in typical ecological areas according to the characteristics of different ecological regions.By studying the relationship between quality traits and various ecological factors,the effects of different ecological factors on the quality of Triticale hay have been clarified,which provides a reasonable basis for future triticale breeding and large-scale and targeted planting layout.The production performance and nutritional qualities of Triticale Trial in Maqu County,Gansu Province and Gannong No.2 in Dulan County,Qinghai Province are obviously superior to other pilot sites.Through reasonable fertilization,the production performance and nutritional quality of triticale are the best.Triticale production performance is significantly related to climatic factors in different pilots.The most suitable planting area for Gannong 2 is Maqu County,Gansu Province.

Characterization of a new hexaploid triticale 6D(6A) substitution line with increased grain weight and decreased spikelet number

Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy plant.Transferring D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is attractive in improving its economically important traits.Here,a hexaploid triticale 6D(6A)substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization(GISH),fluorescence in situ hybridization(FISH),and molecular markers.The GISH analysis showed that Lin 456 is a hexaploid triticalewith 14 rye(Secale cereale)chromosomes and 28 wheat chromosomes,whereas non-denaturing fluorescence in situ hybridization(ND-FISH)and molecular marker analysis revealed that it is a 6D(6A)substitution line.In contrast to previous studies,the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin 456.The wheat chromosome 6D was associatedwith increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three environments.The thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three environments.We propose that the hexaploid triticale 6D(6A)substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.

Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale×wheat derived doubled haploid lines

The rye genome has shown potential for improvement of bread wheat,where wheat-rye substitutions and translocations have been and are frequently used in resistance breeding.Crosses belongs to different generations viz.,BC_(1)F_(1),BC_(1)F_(2),BC_(1)F_(3),BC_(1)F_(4)and BC_(2)F_(3)of triticale×wheat derived were used for different haploid induction parameters using Gogon grass(Imperata cylindrica)as a pollen source.The percentage of pseudo seed formation ranged from 34.55%for BC_(1)F_(2)to 63.77 for BC_(1)F_(1)crosses,the haploid embryo formation ranges from 9.43%for BC_(1)F_(1)to 30.2%for BC_(1)F_(2),the haploid plant generation ranges from 19.36%for BC_(1)F_(2)to 63.25%for BC_(1)F_(1).Four doubled haploids were developed from ITSN 105/58-VL 802-VL 802 of BC_(2)F_(3)underwent molecular cytogenetic analyses using the probes,viz.,rye genomic rDNA,pSc 119 and pAs1.FISH and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.

Hungaro Durum Rye—Breeding, Genome Composition and Amino Acid Content of the First Triticale Cultivar for Human Consumption

Hungaro triticale cultivar was bred for human consumption and animal fodder in Kisvárda, Hungary. The breeding method applied for the recombinant secondary hexaploid triticale proved to be effective since the favourable traits of wheat, durum wheat and rye were successfully combined in the genome of the new cultivar. The first aim was to characterize the genome composition. Multicolour genomic in situ hybridization (mcGISH) was used for the genome compositional analysis. According to the results, the cultivar contains the wheat AB genome (AABB = 28 chromosomes) and the whole rye (R) genome (RR = 14 chromosomes). The presence of D genome, however, could not be detected within the detection limit of the GISH method. Hungaro is the very first triticale in Hungary that has been used for human consumption (milling industry, baking industry, pasta production). In this study, amino acid content of flours was measured. Amino acid investigations on Hungaro durum rye’s flour showed that the quantity of essential amino acids, especially of methionine and cysteine, are higher in Hungaro durum rye than in the also investigated Ryefood rye cultivar and the GK ?thalom wheat cultivar. The genes inherited from the wheat species ensure high yield and great baking and dough making qualities. Therefore, its flour is suitable in itself for pastry and dough production (without mixing it with other flours). Its characteristics inherited from rye guarantee excellent abiotic and biotic resistance.

Optimal Extraction,Purification and Activity Determination of Phytase from Triticale

[ Objective] This study aimed to optimize the extraction conditions of high-activity phytase from triticale. [ Method] Food and forage triticale 209 was used as an experimental material to investigate the optimal extraction conditions, including pH, solid-liquid ratio, extraction duration and active agent concentra- tion. The extracted phytase was purified with salting-out concentration method for SDS-PAGE eIectrophoresis Total protein content was measured using Bradford method; phytase activity was measured using vanadium ammonium molybdate method in accordance with the national standard GB/T 18634 -2009. [ Result] Phytase activity reached the highest under extraction conditions of pH 5.0, solid-liquid ratio 10, room temperature, shaking speed 200 r/min and shaking duration 1 h, without addition of active agems. [ Condusion] This study improved the extraction technology of phytase from wheat plants and was suitable for practical ap- plication.

Evaluation Forage Clipping Stages and Different Levels of Nitrogen on Grain and Forage Yields of TRITICALE (×TRITICOSECALE WITTMACK)

In order to reach suitable forage clipping and estimate nitrogen fertilizer to most economical proficiency, an experiment was carried out at Saatlo Station in 2008-2009 seasons. A factorial experiment was done based on the randomized complete blocks design with 4 replications. The first factor was three levels of clipping stages including non-clipping, clipping at tillering and booting stages. Different levels of nitrogen fertilizer including 0, 40, 80, 120 and 160 kg/ha were arranged at the second factor. Results showed that non-clipping and clipping at booting stage with 610.5 and 203.1 g/m2 had the most and the lowest grain yield, respectively. The highest economical proficiency (3743.06 $/ha) resulted with using 80 kg/ha nitrogen fertilizer and non-clipping. With considering both forage and grain yield, it is necessary that the clipping should be done at tillering stage with using of 120 kg/ha nitrogen fertilizer (3336.56 $/ha). At regression of economical proficiency traits of grain yield, straw and total dry matter remained at the final model. Economical proficiency had the significant positive correlation with spike per square meter (0.87**), grain yield (0.64**), total dry matter (0.65**), plant height (0.52**) and 1000-kernal weight (0.54**).

The association between the allelic state of <i>Vp</i>-1<i>B</i>and pre-harvest sprouting tolerance in red-seeded hexaploid triticale

The core collection of red-seeded winter hexaploid triticale with different pre-harvest sprouting (PHS) resistance has been evaluated for the allelic structure of the gene VIVIPAROUS-1B (Vp-1B) with STS molecular marker. The discovered structure of the collection was as follows: 50.0% and 41.7% of the collection carries Vp-1Bа and Vp-1Bc alleles, respectively, while 8,3% possesses both of them. As a result of the seed color estimation, the collection has been divided into two groups: with dark red seeds and light red seeds. The allele Vp-1Bc has appeared to be associated with PHS resistance while Vp-1Ba with PHS susceptibility in the triticale accessions with light red seeds only. The influence of the seed color and allelic state of Vp-1B on PHS resistance in triticale is discussed.

Hairy Vetch and Triticale Cover Crops for N Management in Soils

Over-application of fertilizer to cropland adversely affects both environmental and agricultural ecosystems. This study examined whether planting a legume-based winter cover crop mix offsets fertilizer application via natural nitrogen inputs. The influence of the cover crop mixture on available nutrients was also assessed. Hairy vetch (Vicia villosa) and winter triticale (×triticosecale) cover crops were planted in fall and terminated in May. Soil fertility data was collected before and after planting the winter cover crop to determine the effect on fixing nitrogen and soil phosphorus, potassium and organic matter levels. Increases of soil ammonium were observed in plots with cover crop treatments. A triticale-hairy vetch cover crop mix was successful at scavenging P for future crops and appears to hold promise for long-term soil fertility benefits.

Efects of Tetcyclacis on Plant Growth and Development in Triticale cv. Lasko

Experiments with tetcyclacis applied as a seed-dressing carried out with triticale cv. lasko in pots in the glasshouse and in hand-sown plots in the field. The results showed that tetcyclacis significantly delayed seedling emergence and inhibited the elongating growth in plant seedling stage, but not affected the growth and development of the adult plant during the late stage of stem extension and the stage of grain expansion. No significant difference was found in fertile stem number, grain quality and yield between the treated plants with tetcyclacis and control.

Adding Value to Crop Production Systems by Integrating Forage Cover Crop Grazing

In addition to their value as cereal grains, wheat (Triticum aestivum L.) and triticale (× Triticosecale Wittmack) are important cool-season annual forages and cover crops. Yearling steer (Bos taurus) performance was compared in the spring following autumn establishment as for age cover crops after soybean [Glycine max (L.) Merr.] grain harvest. Replicated pastures (0.4 ha) were no-till seeded in three consecutive years into soybean stubble in autumn, fertilized, and grazed the following spring near Ithaca, NE, USA. Each pasture (n = 3) was continuously stocked in spring with four yearling steers (380 ± 38 kg) for 17, 32, and 28 d in 2005, 2006, and 2007, respectively. In 2005, average daily gain (ADG) for steers grazing triticale exceeded the ADG for wheat by 0.31 kghd-1d-1. In 2006, wheat ADG exceeded that for triticale by 0.12 kghd-1d-1. In 2007, steers grazing wheat lost weight, while steers grazing triticale gained 0.20 kghd-1d-1. Based on the 3-year average animal gains valued at $1.32 kg-1, mean net return ($ ha-1 yr-1) was $62.15 for triticale and $22.55 for wheat. Since these grazed cover crops provide ecosystem services in addition to forage, grazing could be viewed as a mechanism for recovering costs and adds additional value to the system. Based on this 3-year grazing trial, triticale was superior to wheat and likely will provide the most stable beef yearling performance across years with variable weather for the western Cornbelt USA.

Study on Refined Triticale Straw Reinforced PP Composites

Triticale straw（TS） and refined triticale straw（RTS） were characterized by scanning electron micro- scope（SEM）, Fourier transform infrared spectroscopy（FTIR） and thermogravimetric analysis（TGA） in detail, and their application in polypropylene（PP） composite was explored. RTS was obtained by refining TS with environmentally benign processes, by which hemicelluloses and lignin were effectively removed. This was proved by FTIR analysis. The effect of cellulose content on the composite thermal stability and mechanical properties was also examined. RTS has better thermal stability than TS because RTS has lower flammable noncellulose ratio. T5%（temperature with mass loss of 5%） of RTS could be more than 200 ℃. It is high enough to meet the requests of processing most polyolefin composites. Both TS and RTS acted as nucleating agents in PP crystallization process. TS was proved to be a filler of the composite, and RTS to be a reinforcing material for the composite. The flexural strength and modulus of RTS/PP increased about by 5% and more than 100%, respectively, compared with those of PP, when the mass fraction of RTS and compatibilizer were 30% and 2%, respectively. The thermal stability of RTS/PP composites was also better than that of TS/PP composites.

Trends of seasonal forage yield changes of triticale in the southern Great Plains of the United States

Background:The focus of triticale breeding in the southern Great Plains of the United States has been to increase forage yield for autumn-winter seasons when most other forage species are dormant.This study aims to estimate the trends of seasonal yield changes over time.Methods:Yield trials had been conducted in a randomized complete block design for up to two decades.Seasonal yield changes over time were estimated using linear regression analysis.Results:The analysis revealed a significant positive relation between autumn forage yield(relative to common checks)and calendar years when the trials were conducted.The estimated improvement of autumn forage yield was about 1.9%per year on medium and heavy soil at Ardmore,Oklahoma,and about 5.4%per year on light soil at Burneyville,Oklahoma.However,winter forage yield change was minimal and the spring forage yield increase was negative,although this decline was not significant.Total forage yield improvements were about 0.6%and 0.7%per year at Ardmore and Burneyville,respectively.Similar results were also observed when years of cultivars'introduction were used instead.Conclusions:The results indicate a significant gain in autumn forage yield,but a minor gain in the total forage yield due to the yield tradeoff between the autumn and spring seasons.

Establishment of a transformation system in close relatives of wheat under the assistance of TaWOX5

Species closely related to wheat are important genetic resources for agricultural production,functional genomics studies and wheat improvement.In this study,a wheat gene related to regeneration,TaWOX5,was applied to establish the Agrobacterium-mediated transformation systems of Triticum monococcum,hexaploid triticale,and rye(Secale cereale L.)using their immature embryos.Transgenic plants were efficiently generated.During the transformation process,the Agrobacterium infection efficiency was assessed by histochemical staining forβ-glucuronidase(GUS).Finally,the transgenic nature of regenerated plants was verified by polymerase chain reaction(PCR)-based genotyping for the presence of the GUS and bialaphos resistance(bar)genes,histochemical staining for GUS protein,and the QuickStix strip assay for bar protein.The transformation efficiency of T.monococcum genotype PI428182 was 94.4%;the efficiencies of four hexaploid triticale genotypes Lin456,ZS3297,ZS1257,and ZS3224 were 52.1,41.2,19.4,and 16.0%,respectively;and the transformation efficiency of rye cultivar Lanzhou Heimai was 7.8%.Fluorescence in situ hybridization(FISH)and genomic in situ hybridization(GISH)analyses indicated that the GUS transgenes were integrated into the distal or near centromere(proximal)regions of the chromosomes in transgenic T.monococcum and hexaploid triticale plants.In the transgenic hexaploid triticale plants,the foreign DNA fragment was randomly integrated into the AABB and RR genomes.Furthermore,the transgene was almost stably inherited in the next generation by Mendel’s law.The findings in this study will promote the genetic improvement of the three plant species for grain or forage production and the improvement of cereal species including wheat for functional genomics studies.

青海省不同海拔生态区饲用小黑麦生产性能分析

为了探究青海省不同海拔生态区3种饲用小黑麦在不同播种时间下的生产性能,本试验设3个不同海拔生态区(乐都生态区,2016 m;湟中生态区,2630 m;同德生态区,3251 m),3个播种时间(早播,适播,晚播)和3个自育品系(ZHS2,ZHSI,GY197),测定和分析3个生态区的3个品系小黑麦在不同播种时间后的生育期、群体动态、草产量、穗部性状、籽粒产量和经济系数等指标的差异。结果表明,ZHS2在乐都生态区,3月5日播种综合生产性能最好;ZHS2在湟中生态区,4月10日播种综合生产性能最好;GY197在同德生态区,4月25日播种综合生产性能最好。本研究结果可作为青海农牧交错区高效种植小黑麦研究提供理论依据。