牛血清白蛋白-PAMAM修饰的开管毛细管电色谱柱的制备

Poly(amidoamine)(PAMAM) dendrimer has a large number of terminal groups which can be functionalized by different kinds of ligands.A novel open-tubular CEC(OT-CEC) column in which BSA was bonded to inner wall of capillary as stationary phase through different generations of PAMAM dendrimer was developed.The obtained columns were characterized via labelling BSA with fluorescein isothiocyanate(FITC).Under the optimized condition,the resolution of D,L-Trp was gradually improved with the increase of PAMAM generation.By OT-CEC column modified with BSA-G3.0-PAMAM,the baseline separation of D,L-Trp can be obtained.

吲哚胺2,3-二氧化酶与免疫系统功能调节

免疫应答受多种因素影响和调节,从而维持机体内环境的稳定。巨噬细胞、树突状细胞等抗原提呈细胞表达的吲哚胺2,3-二氧化酶(Indoleam ine 2,3-d ioxygenase,IDO)清除局部微环境中的色氨酸,诱导T淋巴细胞的凋亡,通过调节性T细胞的作用抑制T细胞的克隆性增生,参于细胞免疫的调节;在机体维持正常的妊娠以及自身免疫性疾病、器官移植排斥反应、肿瘤等疾病的发生发展中起着重要作用。调控IDO的活性可能是寻找防治免疫相关性疾病药物的新途径。

加锡果宁抗惊厥作用的机理

采用小鼠最大电休克惊厥法(MES),应用工具药物分析了加锡果宁(Ed)抗小鼠惊厥作用机理.结果,Ed具有较强的抗小鼠MES作用,其作用能被利血平、对氯苯丙胺、氟哌啶醇所对抗,被帕吉林、丙磺舒、L-色氨酸及L-多巴所增强.但α-甲基酪氨酸和酚妥拉明对其抗惊厥作用无明显影响。表明Ed抗惊厥作用与脑内5-MT,DA神经递质有关。