A 3D femoral model was built to obtain the three-dimensional temperature distribution of femur and its surrounding tissues and provide references for clinical applications. According to the relationship between gray-v...A 3D femoral model was built to obtain the three-dimensional temperature distribution of femur and its surrounding tissues and provide references for clinical applications. According to the relationship between gray-value and material properties,the model was assigned with various materials to make sure that it is more similar to the real femur in geometry and physical properties. 3D temperature distribution is obtained by using finite element analysis software ANSYS 11. 0 on the basis of heat conduction theory,Laplace equation,Pennes bio-heat transfer equation,thermo physical parameters of bone tissues,the boundary condition,and initial conditions. Taken the asymmetry of the 3D distribution of temperature into account,it is necessary to adopt the heating method with multiple heat sources. This method can ensure that the temperature fields match well with the tumor tissues and kill the tumor cells efficiently under the condition of protecting the normal tissues from damage. The analysis results supply important guidance for determining the needle position and the needle number and controlling the intensity of heating.展开更多
B16 melanoma cells were inoculated into the BALB/C mice to establish melanoma-bearing models. The antitumor effect of Tween-80 in combination with hyperthermia 41℃ was studied. We observed the changes of the mortalit...B16 melanoma cells were inoculated into the BALB/C mice to establish melanoma-bearing models. The antitumor effect of Tween-80 in combination with hyperthermia 41℃ was studied. We observed the changes of the mortality of tumor-bearing mice, the tumor growth curves, activities of serum tumor necrosis factor (STNF) and the level of serum sialic acid (SSA) in tumor-bearing mice. The number of pulmonary metastatic tumor foci from blood flow was also detected. The results showed that combined with Twecn-80, hyperthermia 41℃ could dramatically suppress the growth of the melanoma in the feet of mice, survive the tumor-bearing mice and decrease the number of pulmonary metastatic tumor foci but no significant effects were observed by treatment with Tween-80 or hyperthermia at 41℃ alone. The activities of STNF and the level of SSA of the melanoma-bearing mice kept at higher levels than those of normal BALB/C mice. Tween-80 combined with heating 41℃ significantly decreased activities of STNF, but the level of SSA still kept high level within 1 to 2 weeks and decreased 10 weeks later with the tumor regression. These results demonstrate that Tween-80 may make hyperthermia exert effective antitumor effect below the critical temperature and increase the safety of hyperthermia in treatment. It is one of the most ideal synergist with hyperthermia. The changes of STNF and SSA suggest that the synergeticeffect could involve in facilitating the exposure of tumor antigen and activation of immune system.展开更多
Thirty pathologically diagnosed patients with grade Ⅲ-Ⅳ primary or recurrent malignant glioma （tumor diameter 3-7 cm） were randomly divided into two groups. The control group underwent conventional radiotherapy an...Thirty pathologically diagnosed patients with grade Ⅲ-Ⅳ primary or recurrent malignant glioma （tumor diameter 3-7 cm） were randomly divided into two groups. The control group underwent conventional radiotherapy and chemotherapy. In the hyperthermia group, primary cases received hyperthermia treatment, and patients with recurrent tumors were treated with hyperthermia in com- bination with radiotherapy and chemotherapy. Hyperthermia treatment was administered using a 13.56-MHz radio frequency hyperthermia device. Electrodes were inserted into the tumor with the aid of a CT-guided stereotactic apparatus and heat was applied for 1 hour. Dudng 3 months a^er hyperthermia, patients were evaluated with head CT or MRI every month. Gliomas in the hyper- thermia group exhibited growth retardation or growth termination. Necrosis was evident in 80% of the heated tumor tissue and there was a decrease in tumor diameter. Our findings indicate that ra- dio frequency hyperthermia has a beneficial effect in the treatment of malignant glioma.展开更多
In this paper, the effects of hyperthermia on cells and immune system are introduced briefly. The mechanism of action of hyperthermia on human papilloma virus （HPV）-infected skin diseases was elaborated as an exampl...In this paper, the effects of hyperthermia on cells and immune system are introduced briefly. The mechanism of action of hyperthermia on human papilloma virus （HPV）-infected skin diseases was elaborated as an example in this paper. Many studies have proved that hyperthermia affects a number of cellular and molecu- lar constitutes in the skin immune system, involving both innate and adaptive immune responses; the efficacy of hyperthermia in treating some infectious and cancerous conditions has been validated and applied in clinics, while molecular mechanisms of hyperthermia affecting the immunereaction is still unclear.展开更多
A computer analysis system, automated imaging cytometry (AIC), flow cytometry (FCM) and EM, were used to study the relationship of apoptosis and the change of DNA in mouse HepA cells subjected to hyperthermia. Our r...A computer analysis system, automated imaging cytometry (AIC), flow cytometry (FCM) and EM, were used to study the relationship of apoptosis and the change of DNA in mouse HepA cells subjected to hyperthermia. Our results showed that pyknotic rate in the 44℃ group was significantly increased (P<0.01) when compared with that in the 37℃ group, both the diploid rate and aneuploid rate in the 44℃ group were significantly decreased (P< 0. 01 ) when compared with that in the 37℃ group. The pyknotic rate determined by ALC was in conformity with the apoptotic index (AI) determined by FCM. Under EM, ultrastructures of HapA cells appeared typical apoptosis changes in the 44℃ group. We are led to conclude that (1) when the temperature is 42℃ or 44℃ for 2 h, it did not promote the necrosis of HepA cells; (2) when the temperature is 42℃ for 2 h, it did not obviously induce the apoptosis of HepA cells, and the 44℃ for 2 h obviously induced the apoptosis of HepA cells; (3) AIC method can be used as an aid for the measurement of apoptotic in- dex. Further study is needed to established it as a novel, simple and convenient method for determin- ing the apoptosis.展开更多
Purpose: In this project, we developed novel methods to quantify changes in tumor hypoxia following a mild tempera-ture hyperthermia (MTH) treatment in rat HT29 human colon adenocarcinoma xenograft. Materials and Meth...Purpose: In this project, we developed novel methods to quantify changes in tumor hypoxia following a mild tempera-ture hyperthermia (MTH) treatment in rat HT29 human colon adenocarcinoma xenograft. Materials and Methods: An exogenous hypoxia marker (IAZGP) was labeled with two radioisotopes of iodine (131I and 123I, respectively) to form two distinct tracers. The two tracers were injected into HT29-bearing nude rats 4-hour before and immediately following 41.5℃, 45-minute mild hyperthermia treatment. The distributions of the two hypoxia tracers were obtained by performing digital autoradiography on tumor sections, and image processing resulted in quantitative information at 50 μm pixel size. Results: Following the hyperthermia treatment, there was a remarkable decrease in hypoxia tracer binding. The average whole tumor hypoxia tracer targeted fraction in five animals changed from 30.3% ± 9.7% to 13.0% ± 5.3% after the hyperthermia treatment (P = 0.001). Detailed pixelby-pixel analysis of the image data revealed a decline in hypoxia tracer uptake after hyperthermia in most regions. However, there was concomitant emergence of some new regions of hypoxia identified by increased tracer uptake. In the control group, the overall hypoxia tracer targeted fraction remained almost constant, with some hypoxic tracer redistribution (putative acute hypoxia) observed. Conclusion: Reoxygenation occurred in the rat HT29 xenograft following MTH treatment. This was evident with preponderance of decreased hypoxia specific tracer uptake on tumor sections. Our methodology might be a useful tool in hypoxia study.展开更多
基金Sponsored by the National Natural Science Foundation of China(Grant No.61272387)the Program for New Century Excellent Talents in University(Grant No.NCET-13-0756)the Distinguished Young Scientists Funds of Heilongjiang Province(Grant No.JC201302)
文摘A 3D femoral model was built to obtain the three-dimensional temperature distribution of femur and its surrounding tissues and provide references for clinical applications. According to the relationship between gray-value and material properties,the model was assigned with various materials to make sure that it is more similar to the real femur in geometry and physical properties. 3D temperature distribution is obtained by using finite element analysis software ANSYS 11. 0 on the basis of heat conduction theory,Laplace equation,Pennes bio-heat transfer equation,thermo physical parameters of bone tissues,the boundary condition,and initial conditions. Taken the asymmetry of the 3D distribution of temperature into account,it is necessary to adopt the heating method with multiple heat sources. This method can ensure that the temperature fields match well with the tumor tissues and kill the tumor cells efficiently under the condition of protecting the normal tissues from damage. The analysis results supply important guidance for determining the needle position and the needle number and controlling the intensity of heating.
基金This Work is supported by National Nature Science Fund (No.39170825 and Shanghai Science Developing Fund.
文摘B16 melanoma cells were inoculated into the BALB/C mice to establish melanoma-bearing models. The antitumor effect of Tween-80 in combination with hyperthermia 41℃ was studied. We observed the changes of the mortality of tumor-bearing mice, the tumor growth curves, activities of serum tumor necrosis factor (STNF) and the level of serum sialic acid (SSA) in tumor-bearing mice. The number of pulmonary metastatic tumor foci from blood flow was also detected. The results showed that combined with Twecn-80, hyperthermia 41℃ could dramatically suppress the growth of the melanoma in the feet of mice, survive the tumor-bearing mice and decrease the number of pulmonary metastatic tumor foci but no significant effects were observed by treatment with Tween-80 or hyperthermia at 41℃ alone. The activities of STNF and the level of SSA of the melanoma-bearing mice kept at higher levels than those of normal BALB/C mice. Tween-80 combined with heating 41℃ significantly decreased activities of STNF, but the level of SSA still kept high level within 1 to 2 weeks and decreased 10 weeks later with the tumor regression. These results demonstrate that Tween-80 may make hyperthermia exert effective antitumor effect below the critical temperature and increase the safety of hyperthermia in treatment. It is one of the most ideal synergist with hyperthermia. The changes of STNF and SSA suggest that the synergeticeffect could involve in facilitating the exposure of tumor antigen and activation of immune system.
基金financially sponsored by the Overseas Returnees of Heilongjiang Province in China,No.IC03C182003-2005
文摘Thirty pathologically diagnosed patients with grade Ⅲ-Ⅳ primary or recurrent malignant glioma （tumor diameter 3-7 cm） were randomly divided into two groups. The control group underwent conventional radiotherapy and chemotherapy. In the hyperthermia group, primary cases received hyperthermia treatment, and patients with recurrent tumors were treated with hyperthermia in com- bination with radiotherapy and chemotherapy. Hyperthermia treatment was administered using a 13.56-MHz radio frequency hyperthermia device. Electrodes were inserted into the tumor with the aid of a CT-guided stereotactic apparatus and heat was applied for 1 hour. Dudng 3 months a^er hyperthermia, patients were evaluated with head CT or MRI every month. Gliomas in the hyper- thermia group exhibited growth retardation or growth termination. Necrosis was evident in 80% of the heated tumor tissue and there was a decrease in tumor diameter. Our findings indicate that ra- dio frequency hyperthermia has a beneficial effect in the treatment of malignant glioma.
文摘In this paper, the effects of hyperthermia on cells and immune system are introduced briefly. The mechanism of action of hyperthermia on human papilloma virus （HPV）-infected skin diseases was elaborated as an example in this paper. Many studies have proved that hyperthermia affects a number of cellular and molecu- lar constitutes in the skin immune system, involving both innate and adaptive immune responses; the efficacy of hyperthermia in treating some infectious and cancerous conditions has been validated and applied in clinics, while molecular mechanisms of hyperthermia affecting the immunereaction is still unclear.
基金This project was supported by the grant of Key Projects ofNational Natural Science Foundation of China (No.59836240).
文摘A computer analysis system, automated imaging cytometry (AIC), flow cytometry (FCM) and EM, were used to study the relationship of apoptosis and the change of DNA in mouse HepA cells subjected to hyperthermia. Our results showed that pyknotic rate in the 44℃ group was significantly increased (P<0.01) when compared with that in the 37℃ group, both the diploid rate and aneuploid rate in the 44℃ group were significantly decreased (P< 0. 01 ) when compared with that in the 37℃ group. The pyknotic rate determined by ALC was in conformity with the apoptotic index (AI) determined by FCM. Under EM, ultrastructures of HapA cells appeared typical apoptosis changes in the 44℃ group. We are led to conclude that (1) when the temperature is 42℃ or 44℃ for 2 h, it did not promote the necrosis of HepA cells; (2) when the temperature is 42℃ for 2 h, it did not obviously induce the apoptosis of HepA cells, and the 44℃ for 2 h obviously induced the apoptosis of HepA cells; (3) AIC method can be used as an aid for the measurement of apoptotic in- dex. Further study is needed to established it as a novel, simple and convenient method for determin- ing the apoptosis.
文摘Purpose: In this project, we developed novel methods to quantify changes in tumor hypoxia following a mild tempera-ture hyperthermia (MTH) treatment in rat HT29 human colon adenocarcinoma xenograft. Materials and Methods: An exogenous hypoxia marker (IAZGP) was labeled with two radioisotopes of iodine (131I and 123I, respectively) to form two distinct tracers. The two tracers were injected into HT29-bearing nude rats 4-hour before and immediately following 41.5℃, 45-minute mild hyperthermia treatment. The distributions of the two hypoxia tracers were obtained by performing digital autoradiography on tumor sections, and image processing resulted in quantitative information at 50 μm pixel size. Results: Following the hyperthermia treatment, there was a remarkable decrease in hypoxia tracer binding. The average whole tumor hypoxia tracer targeted fraction in five animals changed from 30.3% ± 9.7% to 13.0% ± 5.3% after the hyperthermia treatment (P = 0.001). Detailed pixelby-pixel analysis of the image data revealed a decline in hypoxia tracer uptake after hyperthermia in most regions. However, there was concomitant emergence of some new regions of hypoxia identified by increased tracer uptake. In the control group, the overall hypoxia tracer targeted fraction remained almost constant, with some hypoxic tracer redistribution (putative acute hypoxia) observed. Conclusion: Reoxygenation occurred in the rat HT29 xenograft following MTH treatment. This was evident with preponderance of decreased hypoxia specific tracer uptake on tumor sections. Our methodology might be a useful tool in hypoxia study.
