In order to investigate the role of Twist gene in the metastasis of hepatocellular carcinoma （HCC）, total RNA was respectively extracted from three HCC cell strains with different metastatic potentials, HepG2, MHCC-...In order to investigate the role of Twist gene in the metastasis of hepatocellular carcinoma （HCC）, total RNA was respectively extracted from three HCC cell strains with different metastatic potentials, HepG2, MHCC-97L and MHCC-97H. The first strand cDNA was synthesized by reverse transcription, which was then used as template to perform fluorescent quantitative polymerase chain reaction （FQ-PCR）. The quantity of Twist gene expression was normalized by that of the housekeeping gene, GAPDH for each sample. ANOVA was used to estimate the relationship between Twist gene and metastasis potential of HCC. The results showed that the normalized initial cDNA concentrations of Twist gene in HepG2, MHCC-97L and MHCC-97H were （9.45±0.25）×10^-4, （ 1.82±0.41 ）× 10^-3, （3.06±0.62）×10^-3, respectively. FQ-PCR revealed significan, t differences in the expression level of Twist among HCC cell strains with different metastatic potentials. It was concluded that high expression level of Twist was closely associated with more aggressive behaviors of HCC. Twist provides a novel indicator for HCC metastasis.展开更多
通过Mitsunobu反应合成了一系列手性液晶,收率63%～71%,并通过1 H NMR、MS、IR对其结构进行了表征。结合DSC和POM研究了目标化合物的介晶性。利用Cano's wedge法研究了目标化合物的扭曲力,发现随着手性中心邻近芳环上氟原子数的增多...通过Mitsunobu反应合成了一系列手性液晶,收率63%～71%,并通过1 H NMR、MS、IR对其结构进行了表征。结合DSC和POM研究了目标化合物的介晶性。利用Cano's wedge法研究了目标化合物的扭曲力,发现随着手性中心邻近芳环上氟原子数的增多,手性液晶分子的扭曲力显著增大。实验结果表明,氟原子的引入影响了碳氧单键的自由旋转,导致手性液晶分子具有较少构象异构体,从而表现出大的扭曲力。展开更多
目的：观察穴位埋线对原发性痛经模型小鼠的影响。方法：取体质量18~22 g雌性成年未孕小鼠40只,随机分成4组,空白对照组、模型组、阿司匹林组、穴位埋线组。实验开始时,空白对照组腹腔皮下注射生理盐水,每日1次（每只0.4 m L）,连续7 d...目的：观察穴位埋线对原发性痛经模型小鼠的影响。方法：取体质量18~22 g雌性成年未孕小鼠40只,随机分成4组,空白对照组、模型组、阿司匹林组、穴位埋线组。实验开始时,空白对照组腹腔皮下注射生理盐水,每日1次（每只0.4 m L）,连续7 d;其余组均连续腹腔皮下注射苯甲酸雌二醇7 d,每日1次（每只0.2 m L）,其中,穴位埋线组在造模第1天予以埋线治疗1次,取穴中极、十七椎下,阿司匹林组于造模第1天开始灌胃给药,连续7 d,每日1次（每只0.2 m L,浓度为0.2 g·m L^-1）,空白对照组和模型组不予以治疗,正常饲养,末次给药1 h后,空白对照组腹腔注射生理盐水每只0.4 m L,其余组腹腔注射缩宫素每只0.2 m L,观察0~20 min内各组小鼠疼痛反应,记录各组扭体次数及扭体发生率。眼眶后静脉丛采血1 m L,迅速注入含有3.8%柠檬酸三钠的塑料指形管中混匀,立即低温离心（4℃3 000 r·min^-1）15 min,取上清,置-70℃低温冰箱,以备PGE2、PG F2α检测。结果：与模型组相比,穴位埋线组小鼠20min内扭体次数显著减少（P〈0.01）,小鼠血浆PGE2含量明显升高（P〈0.01）,小鼠血浆PGF2α含量明显降低（P〈0.01）,差异有统计学意义;与阿司匹林组相比,差异无统计学意义（P〉0.05）。结论：穴位埋线治疗原发性痛经具有明显的镇痛作用;穴位埋线疗法有调节血浆前列腺素分泌的作用。展开更多
文摘In order to investigate the role of Twist gene in the metastasis of hepatocellular carcinoma （HCC）, total RNA was respectively extracted from three HCC cell strains with different metastatic potentials, HepG2, MHCC-97L and MHCC-97H. The first strand cDNA was synthesized by reverse transcription, which was then used as template to perform fluorescent quantitative polymerase chain reaction （FQ-PCR）. The quantity of Twist gene expression was normalized by that of the housekeeping gene, GAPDH for each sample. ANOVA was used to estimate the relationship between Twist gene and metastasis potential of HCC. The results showed that the normalized initial cDNA concentrations of Twist gene in HepG2, MHCC-97L and MHCC-97H were （9.45±0.25）×10^-4, （ 1.82±0.41 ）× 10^-3, （3.06±0.62）×10^-3, respectively. FQ-PCR revealed significan, t differences in the expression level of Twist among HCC cell strains with different metastatic potentials. It was concluded that high expression level of Twist was closely associated with more aggressive behaviors of HCC. Twist provides a novel indicator for HCC metastasis.
文摘通过Mitsunobu反应合成了一系列手性液晶,收率63%～71%,并通过1 H NMR、MS、IR对其结构进行了表征。结合DSC和POM研究了目标化合物的介晶性。利用Cano's wedge法研究了目标化合物的扭曲力,发现随着手性中心邻近芳环上氟原子数的增多,手性液晶分子的扭曲力显著增大。实验结果表明,氟原子的引入影响了碳氧单键的自由旋转,导致手性液晶分子具有较少构象异构体,从而表现出大的扭曲力。
文摘目的：观察穴位埋线对原发性痛经模型小鼠的影响。方法：取体质量18~22 g雌性成年未孕小鼠40只,随机分成4组,空白对照组、模型组、阿司匹林组、穴位埋线组。实验开始时,空白对照组腹腔皮下注射生理盐水,每日1次（每只0.4 m L）,连续7 d;其余组均连续腹腔皮下注射苯甲酸雌二醇7 d,每日1次（每只0.2 m L）,其中,穴位埋线组在造模第1天予以埋线治疗1次,取穴中极、十七椎下,阿司匹林组于造模第1天开始灌胃给药,连续7 d,每日1次（每只0.2 m L,浓度为0.2 g·m L^-1）,空白对照组和模型组不予以治疗,正常饲养,末次给药1 h后,空白对照组腹腔注射生理盐水每只0.4 m L,其余组腹腔注射缩宫素每只0.2 m L,观察0~20 min内各组小鼠疼痛反应,记录各组扭体次数及扭体发生率。眼眶后静脉丛采血1 m L,迅速注入含有3.8%柠檬酸三钠的塑料指形管中混匀,立即低温离心（4℃3 000 r·min^-1）15 min,取上清,置-70℃低温冰箱,以备PGE2、PG F2α检测。结果：与模型组相比,穴位埋线组小鼠20min内扭体次数显著减少（P〈0.01）,小鼠血浆PGE2含量明显升高（P〈0.01）,小鼠血浆PGF2α含量明显降低（P〈0.01）,差异有统计学意义;与阿司匹林组相比,差异无统计学意义（P〉0.05）。结论：穴位埋线治疗原发性痛经具有明显的镇痛作用;穴位埋线疗法有调节血浆前列腺素分泌的作用。
