Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis

A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown planthopper ( BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two-dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 ( pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of lice plants to BPH infestation.

Experimental Investigation of the Effect of Bow Profiles on Resistance of an Underwater Vehicle in Free Surface Motion

In this paper, towing tank experiments are conducted to study the behavior of flow on a model of the underwater vehicle with various shapes of bows, i.e. tango and standard bows in free surface motion tests. The total resistances for different Froude numbers are considered experimentally. The towing tank is equipped with a trolley that can operate in through 0.05-6 m/s speed with ±0.02 m/s accuracy. Furthermore, the study is done on hydrodynamic coefficients i.e. total, residual and friction resistance coefficients, and the results are compared. Finally, the study on flow of wave fields around bows is done and wave filed around two bows are compared. The Froude number interval is between 0.099 and 0.349. Blockage fraction for the model is fixed to 0.005 3. The results showed that the residual resistance of the standard bow in 0.19 to 0.3 Froude number is more than the tango bow in surface motion which causes more total resistance for the submarine. Finally, details of wave generated by the bow are depicted and the effects of flow pattern on resistance drag are discussed.

Characterization of Aquifers in Crystalline and Crystallophyll Basement Zones Using the Electrical Resistivity Method (Trails and Electrical Soundings) in the Gagnoa Region, (Central-Western Côte d’Ivoire)

Introduction: Located in the central-western part of Côte d’Ivoire, the subsoil of the Gagnoa region is made up of sedimentary volcano formations and granitoids with developed fracturing. This complex Precambrian basement contains most of the region’s water resources. This is at the origin of the high failure rate during the various hydrogeological prospecting campaigns. Methodology: The database consists of resistivities from 42 holes and 51 trails drilled as part of the implementation of high-throughput drilling in the study area. The objective of this study is to deepen the knowledge of the fissured basement by interpreting profile curves and electrical soundings. It will be a question of classifying the different types of anomalies obtained on the profiles and their shapes. The orientation of the lineaments observed on the profiles was determined. Results: The interpretation of the geophysical data revealed various anomalies, the main ones being of the CC (Conductor Compartment) and CEDP (Contact between two bearings) types. These types of anomalies are mainly expressed in various forms: the “V”, “W” and “U” shapes. From these anomalies and the appearance of the electrical profiles, lineaments and their orientations were identified with N90-100, N130-140, N170-180 as major orientations. Conclusion: These results could contribute to a better understanding of the fractured environment of the Gagnoa region.

Atherogenic lipids profile relates to postprandial hyperglycemia and hyperinsulinemia due to whole body insulin resistance in prediabetic subjects

Backgrounds: Differences in serum lipids profiles in different type of glucose intolerance are unclear. Aims: To characterize lipid profiles in different type of glucose intolerance, and to assess relationships between serum lipids profile and disturbance of glucose metabolism in prediabetic subjects. Methods: Using the measurements in medical check-up with 75 goral glucose tolerance test (OGTT), total of 620 male subjects, who are not on medications for metabolic diseases or hypertension, were divided into normal fasting glucose and glucose tolerance (NFG/ NGT), isolated impaired fasting glucose (iIFG), isolated impaired glucose tolerance (iIGT) and combined IFG and IGT (IFG/IGT) based on results of the OGTT. Results: Age and body mass index (BMI) were similar in the four groups. Matsuda index (an index of whole body insulin sensitivity) was lower in iIFG, iIGT and IFG/IGT as compared with NFG/NGT. Plasma insulin excursion during the OGTT was significantly higher in IFG/IGT versus NFG/NGT. Serum triglyceride level (TG) and TG to HDL ratio (TG/HDL) were higher in IFG/IGT versus NFG/NGT. Matsuda index was positively correlated with HDL and was inversely correlated with TG, LDL, non-HDL, TG/ HDL and LDL to HDL ratio (LDL/HDL). Backward stepwise multiple regression analysis indicated that increases in BMI, plasma insulin level at 60 min (PI60) and plasma glucose level at 120 min in the OGTT were independently associated with increases in TG and TG/HDL. Increases in BMI and PI60 were related to an increase in non-HDL and LDL/HDL and a decrease in HDL. Conclusions: These results indicate that postprandial hyperglycemia and hyperinsulinemia based on advanced insulin resistance are closely related to lipid risk factors of atherosclerotic macrovascular disease in prediabetic subjects.

Antimicrobial Resistance,Virulence Profile,and Molecular Characterization of Listeria monocytogenes Isolated from Ready-to-eat Food in China,2013-2014

We aimed to investigate the potential pathogenic profile and antibiotic resistance of Listeria monocytogenes isolated from ready-to-eat food in China.Antimicrobial resistance was determined by broth microdilution following the Clinical and Laboratory Standards Institute protocol Molecular serotyping,virulence,and resistance genes were identified using PCR.Multi-locus

Effects of Plasma Density Profile on Resistive Wall Mode Stability

Linear and nonlinear evolutions of the resistive wall mode(RWM) were numerically carried out with different plasma density profiles.Both stabilizing and destabilizing effects were observed.The plasma density shear had a stabilizing effect on the RWM,and the stabilizing effect was better as the plasma density shear rate increased.In the uniform density plasma,the effects of flowshear on the RWMstability were stronger than those for non-uniform density plasma.For lower flowshear,the effects of the plasma density shear rate on the RWMwere more obvious.In the nonlinear phase,the repulsive force associated with the piling up magnetic flux near the resistive wall prevented further growth of perturbed magnetic energy,and the RWMwas saturated.The saturation levels were almost independent of the density profiles.

Antimicrobial Drug Resistance and Plasmid Profiles of <i>Salmonella</i>Isolates from Humans and Foods of Animal Origin in Uganda

Salmonella organisms are among the most common causes of human bacterial gastroenteritis worldwide, and food animals are important reservoirs of these bacteria. A further danger lies in the development of drug resistance in these organisms, primarily driven by non-prudent overuse of antiinfectives. The current study compared the plasmid profiles and drug susceptibility patterns of Salmonella isolates from man and foods of animal origin in Uganda. A total of 92 Salmonella isolates (58 from man and 34 from foods of animal origin) were analyzed. Identification was done by using biochemical tests;plasmid profiling by agarose gel electrophoresis while susceptibility testing to tetracycline, ampicillin, chloramphenicol, nalidixic acid, trimethoprim-sulphamethoxazole, ciprofloxacin, ceftriaxone and tetracycline were done by the Kirby Bauer Disc Diffusion method. Among the human isolates, 57/58 (98.3%, 95% CI, 91.8% - 99.9%) were susceptible to Ciprofloxacin compared to 32/34 (94.1%, 95% CI, 81.9% - 91%) of animal-derived isolates. On the other hand, 48/58 (82.7%, 95% CI, 71.4% - 91%) human-derived isolates were resistant to Trimethoprim sulfamethoxazole compared to 29/34(85.3%) of the animal-derived isolates. Fifty four percent (n = 50) of the all the isolates were resistant to at least three antibiotics whereas only 2.2% (n = 2) were susceptible to all the seven drugs tested. Most worrying, however, was the fact that only 45% percent of the isolates were sensitive to all the three drugs (chloramphenicol, nalidixic acid and ciprofloxacin) commonly used in the treatment of salmonellosis in this setting. The risk of ampicillin resistance was three times more likely to occur in animal-derived as compared to human-derived isolates (Odds Ratio = 2.705, 95% CI, 1.3 - 5.8) as was that to nalidixic acid (Odds Ratio = 2.895, 95% CI, 1.17 - 7.2). Plasmid profile analysis showed eight clusters comprising of 68.7% (46/67) of the isolates. In five of the eight clusters, there were both animal and human-derived isolates. Resistant strains of Salmonella are common in this setting and meat/meat products are the commonest source of infection. A majority of the isolates are multi-drug resistant, and there is evidence of cross-species transmission of plasmids, and possibly drug resistance, between animals and humans.

Prevalence and Antibiotic Profile of Multidrug Resistance Gram-Negative Pathogens Isolated from Wound Infections at Two Tertiary Hospitals in Limpopo Province, South Africa: A Retrospective Study

Background: There is a high mortality and morbidity associated with multidrug resistance pathogens. The high rate of MDR isolates is gradually becoming a threat to the coverage of antibiotics including the clinical outcome of infected patients. Methods: A 5-year laboratory based antibiotic susceptibility data from January 2016 to December 2020 was reviewed to determine the most prevalent MDR pathogens isolated from samples taken from patients with wound infections at Pietersburg and Mankweng Hospitals. Results: A total of 792 pathogens were analyzed. The most prevalent isolates were Klebsiella pneumoniae (23%), Pseudomonas aeruginosa (21.7%), Escherichia coli (16%), Proteus mirabilis (13.5%), Acinetobacter baumannii (9.1%) and Klebsiella oxytoca (3.8%). The overall prevalence of MDR isolates in this study was 38.1%. The distribution of MDR prevalence amongst these isolates was K. pneumoniae (20.8%), P. aeruginosa (18.8%), P. mirabilis (17.5%), E. coli (15.9%) and A. baumannii (8.3%). Of the 9 antibiotic agents tested, trimethoprim sulfamethoxazole, tigecycline and cefepime had the highest levels of resistance. The highest level of resistance was conferred by K. pneumoniae having 63% of isolates identified as MDR. The study could not determine statistical significance of any determinant of MDR. Conclusion: The study revealed that there is high rate of MDR pathogens (38.9%) in Limpopo. Majority of the pathogens were K. pneumoniae from surgical ward, which also conferred high resistance levels. A proportion of the MDR pathogens was identified as A. baumannii CRE pathogens and is noteworthy in implementation aggressive infection prevention and control strategies.

Study and Application of a New Association Polymer System for Profile Control

A new polymer system, referred to simply as the AP-P4 polymer system, aims at solving the problems of high temperature, high salinity and the poor shearing resistance, all of which are encountered by conventional polymers (such as polyacrylamide) used in profile control, profile performance improvement and EOR operations in the Zhongyuan Oilfield, Sinopec. This system has been developed on the basis of the specific molecular structure and the better properties of high temperature resistance, high salinity resistance and strong shearing resistance of the new type of AP-P4 association polymer. Acidity modifying agents and cross-linking agents (MZ-YL, MZ-BE, MZ-XS), compatible with the new polymer system, are selected. Results of performance tests have shown that the new polymer system has excellent thickening, high temperature, high salinity and shearing resistance and anti-dehydrating properties. In 2003, it underwent its first pilot test in 26 wells in China, with remarkable effects in increasing oil production and decreasing water production. The newly developed polymer system and its application technology described in this paper may play a guiding role in polymer profile control operations in the oil reservoirs of high temperature and high salinity.

Effects of single and multi-stage solid solution treatments on microstructure and properties of as-extruded AA7055 helical profile

The effects of single-stage solution treatment(SST),enhanced solution treatment(EST),high-temperature pre-precipitation(HTPP)and multi-stage solution treatment(MST)on the microstructure,mechanical properties and corrosion resistance of the as-extruded 7055 aluminium alloy(AA7055)helical profile were investigated using differential scanning calorimetry(DSC),optical microscopy(OM),scanning electron microscopy(SEM),electron back-scattered diffraction(EBSD)and transmission electron microscopy(TEM).It was observed that EST and MST could promote the dissolution of the second-phase particles compared with the traditional SST,and the intergranular phases were distinctly discontinuously distributed after HTPP and MST.There was obvious difference in the main texture type and texture strength for the alloy after different solid solution treatments.HTPP could improve the corrosion resistance of the alloy by regulating the intergranular phases,but the mechanical properties were severely weakened.While the good corrosion resistance of the alloy could be obtained by MST without obvious strength loss.As a result,the MST is an ideal solid solution treatment scheme for AA7055.

NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes, is the most efficient approach to control late blight. Solanum demissum is a well-known wild potato species from which 11 race-specific resistance genes have been identified, however, no broad-spectrum resistance genes like RB have been reported in this species. Here, we report a novel reisistance locus from S. demissum that potentially confer broad-spectrum resistance to late blight. A small segregating population of S. demissum were assessed for resistance to aggressive P. infestans isolates（race 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11）. This coupled with nucleotide binding site（NBS） profiling analyses, led to the identification of three fragments that linked to the potential candidate resistance gene（s）. Cloning and sequence analysis of these fragments suggested that the identified resistance gene locus is located in the region containing R2 resistance gene at chromosome 4. Based on the sequences of the cloned fragments, a co-segregating sequence characterized amplified region（SCAR） marker, RDSP, was developed. The newly identified marker RDSP will be useful for marker assisted breeding and further cloning of this potential resistance gene locus.

Experimental Investigation of Two-Dimensional Velocity on the 90&deg;Double Bend Pipe Flow Using Ultrasound Technique

An experimental investigation was performed to investigate two-dimensional axial velocity field at downstream of the 90&deg;double bend pipe with and without inlet swirling condition. The main objectives are to find separation region and observe the influence of inlet swirling flow on the velocity fluctuation using ultrasound technique. The experiments were carried out in the pipe at Reynolds number Re = 1 × 104. In case of inlet swirling flow condition, a rotary swirler was used as swirling generator, and the swirl number was setup S = 1. The ultrasonic measurements were taken at four downstream locations of the second bend pipe. Phased Array Ultrasonic Velocity Profiler (Phased Array UVP) technique was applied to obtain the two-dimensional velocity of the fluid and the axial and tangential velocity fluctuation. It was found that the secondary reverse flow became smaller at the downstream from the bend when the inlet condition on the first bend was swirling flow. In addition, inlet swirling condition influenced mainly on the tangential velocity fluctuation, and its maximum turbulence intensity was 40%.

Antibiotics Resistance Pattern and Plasmid Profiling of <i>Edwardsiella tarda</i>Isolated from <i>Heterobranchus longifilis</i>

A study was carried out to investigate antibiotic resistance patterns and plasmid profiling of Edwardsiella tarda isolated from farmed-cultured Heterobranchus longifilis in Lagos State, Southwest of Nigeria. A total of 44 Edwardsiella isolates were recovered from 80 fish samples collected from the 10 fish farms using selective random stratification. It was observed that Edwardsiella tarda isolates were 100% resistant to Amoxicillin, Chloranphenicol, Levofloxacin, Streptomycin and 90% resistant to Nalidixic Acid respectively. All the isolates were 100% susceptible to Spectinomycin and Ciprofloxacin, while Ofloxacin, Gentamycin, and Pefloxacin vary in their level of susceptibility with 90%, 80% and 70% sensitivity respectively. Conversely, 8 out of 10 fish farm locations studied were observed to have antibiotic-resistant strains, and 5 out of 8 drug-resistant strains were found to carry plasmid and the sizes of the plasmid ranges between 20.027 kb to 23.130 kb. The plasmid after treatment with mitomycin C and ethidium bromide were lost during the process of plasmid curing confirming that the multiple drug resistant exhibited by the isolates was plasmid mediated. There are fewer studies on antibiotic resistance in Edwardsiella tarda from aquaculture enterprises and this study provides further support to the view that there is a potential risk of transfer of resistant bacteria and their genes to human pathogen through the food chain. Although, in Nigeria there is no antibiotic product registered for aquaculture usage, yet fish farmers use them off-label for bacterial diseases prevention.

基于OperationalProfile的船体首部参数化建模及优化

以一艘多用途船为例，介绍了考虑多个航行工况的线型优化方法。首先，根据船东预期的实船营运情况，制定表明目标船多点优化目标的operationalprofile；然后，对船体首部进行参数化建模与变换，并通过兴波阻力计算优化得到满足operationalprofile优化目标的船体首部参数化模型。

Effect of the Type of Vitis vinifera Cultivation in the Cenophenoresistome and Metabolic Profiling （CLPP） of Edaphic Bacterial Communities

In the present work, bacterial soil communities of different grapevine exploitation samples are studied in order to elucidate the possible influence of different agrarian management techniques （use of fertilizers, irrigation with river water） may have on the rhizospheric microbiome of Vitis vinifera plants. Therefore, it is postulated the Cenophenoresistome as a novel methodology to evaluate complex communities＇ global resistance against different antibiotics, by using and adjusting a serial of techniques traditionally applied to evaluate a monospecific population＇s resistance against antibiotics （Vitek, ATB and disk diffusion methods）. Likewise, the metabolic profile （CLPP： comunity level physiological profile） of bacterial communities is studied by Biolog ECO. In relation to the functional structure of the bacterial communities, it is observed that the metabolic profile （diversity, kinetics and CLPP） of unexploited soils differs from soils under anthropic influence. It is discussed the causes of resistance in the human clinic antibiotic treatment based on the agrarian management, especially with the contamination transmitted by irrigation water, which could be associated with changes in edaphic communities. The results obtained in the present study through two different approaches （Cenophenoresistome and metabolic profiles） are consistent with each other, suggesting that both methods can be good bioindicators of the state of humankind-altered soils that host natural ecosystems. Likewise, the concept of Cenophenoresistome is proposed as a bioindicator of soil response to alteration processes, as well as a possible predictor of its evolution in edaphic remediation processes.

Urinary Tract Infection among Adults Seeking Medicare at Kiambu Level 5 Hospital, Kenya: Prevalence, Diversity, Antimicrobial Susceptibility Profiles and Possible Risk Factors

Urinary tract infections are among the most prevalent extra-intestinal infections, with high prevalence globally. This cross-sectional study established prevalence of bacterial aetiology causing urinary tract infection (UTI) and their antimicrobial susceptibility profiles. A questionnaire was used to capture socio-demographic data and possible UTI risk factors among the 206 consented adults seeking medicare at Kiambu Level 5 Hospital. The collected midstream urine samples were subjected to dipstick analysis, microscopy and culture for UTI diagnosis. Results: The overall prevalence rate of UTIs was 27.6%, with women’s prevalence rate being significantly higher at 80.7% compared to men 19.2%. Pregnant women had UTI prevalence at 34% which was higher than other sets of participants. Women who did not frequently change their underpants daily had a higher UTI cases at 34.8%. Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae were the most prevalent bacterial pathogens at 38.5%, 21% and 19.3%, respectively. Antimicrobial sensitivity analysis revealed high resistances towards Sulfamethoxazole and Ampicillin at range between 50% - 85%, suggesting that these drugs are no longer effective for UTI empirical treatment. The resistance patterns towards Cefotaxime, Cefepime and Ciprofloxacin were below 40%. However, more resistance patterns at a range between 14% - 40% revealed towards Amoxicillin-clavulanic and Nitrofurantoin imply that these are drugs remain potent but there is the need to revise the current UTI management guidelines. In addition, to elude treatment failure, innovation of prophylactic measures is key to halt UTI contraction and offer support to pharmaceutical industries that have fewer new antibiotics in the pipeline.

Electrical Resistivity Survey on Two Waste Dumpsites at Nguru,Potiskum,Yobe State,Nigeria to Determine the Effect of Leachates on Ground Water Aquifer

The research intends to bring out the contribution of leachate on groundwater in two dumpsites in Nguru and Potiskum all in Yobe state,Nigeria.A total of seven(7)and eight(8)VES by Schlumberger electrode with the use of Wenner electrode configuration.The results were interpreted by the use of WinRESIST for VES and IPWIN2INV for ERT.The study pointed out that,the area in question is comprised of four layers of geoelectric such as the topsoil,clay,sand,sandy clay and sand.The range of the first resistivity layer was from 6.16Ωm to 332Ωm in the first geo-electric layer and its thickness range from 2.77 m to 37.7 m and a depth range of 2.77 m to 37.7 m.the range of the second resistivity layer was from 16.5Ωm to 37.9Ωm which has the range of its thickness from 4.1 m to 10.7 m.The range of the third resistivity layer was from 101.2Ωm to 288.2Ωm which has the range of its thickness from 38.9 m to 99.7 m,and the first aquifer in the area.The range of the first resistivity layer was from 100.7Ωm to 214.3Ωm which has the range of its thickness from 28.5 m to 94 m.The fifth layer which is the second aquifer and has resistivity from 254Ωm to 350Ωm with a very large thickness.The range of the first resistivity aquifer is from 101.2Ωm to 288.2Ωm and the range of the second resistivity aquifer is from 253.8Ωm to 350.1Ωm.The 2D ERT profiles unveiled areas with low resistant zones and later discussed as zones penetrated by contaminants originated from dumpsites whereas high resistant zones represent areas of low or non-conductive materials in the area.Data obtained from four dumpsites indicated that leachate of the waste dumpsites penetrated into aquifers and polluted the groundwater.The existence of contaminants in the water was noted by a decrease in the formation resistant values.It is seen,from the results of the survey(geophysical)that the water in the area is polluted and it accounts for the prevalence of any disease related to water that are common in the area.

Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway

WRKY transcription factors,transcriptional regulators unique to plants,play an important role in defense response to pathogen infection.However,the resistance mechanisms of WRKY genes in sugarcane remain unclear.In the present study,gene ontology(GO)enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in defense response.We identified gene ScWRKY4,a classⅡc member of the WRKY gene family,in sugarcane cultivar ROC22.This gene was induced by salicylic acid(SA)and methyl jasmonate(MeJA)stress.Interestingly,expression of ScWRKY4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smutsusceptible sugarcane cultivars infected with Sporisorium scitamineum.Moreover,stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani var.coeruleum and caused down-regulated expression of immune marker-related genes.Transcriptome analysis indicated suppressed expression of most JAZ genes in the signal transduction pathway.ScWRKY4 interacted with ScJAZ13 to repress its expression.We thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance,most likely through the JA signaling pathway.The present study depicting the molecular involvement of ScWRKY4 in sugarcane disease resistance lays a foundation for future investigation.

Development and Identification of Novel Rice Blast Resistant Sources and Their Characterization Using Molecular Markers

To develop and characterize introgression lines for leaf and neck blast resistance, 326 introgression lines were developed using various accessions of six different AA genome wild species in the genetic background of elite Indian varieties like PRl14 and Pusa 44 and were screened for blast resistance. Stringent phenotyping coupled with genotyping using gene based markers led to the identification of four resistant introgression lines, which showed promising resistance and do not possess any of the tested genes. Furthermore, multi-location screening confirmed the field resistance of the four introgression lines to both leaf and neck blast. Molecular characterization of these introgression lines using genome-wide simple sequence repeat markers revealed the presence of small percentage of wild Oryza genome introgrssion. So these lines can be used for mapping and identification of novel leaf and neck blast resistance genes. Thus, these four introgression lines can be considered as new genetic resources for blast resistance.

Cancer epigenetics: a perspective on the role of DNA methylation in acquired endocrine resistance

Epigenetic mechanisms, including DNA methylation, are responsible for determining and maintaining cell fate, stably differentiating the various tissues in our bodies. Increasing evidence shows that DNA methylation plays a significant role in cancer, from the silencing of tumor suppressors to the activation of oncogenes and the promotion of metastasis. Recent studies also suggest a role for DNA methylation in drug resistance. This perspective article discusses how DNA methylation may contribute to the development of acquired endocrine resistance, with a focus on breast cancer. In addition, we discuss DNA methylome profiling and how recent developments in this field are shedding new light on the role of epigenetics in endocrine resistance. Hormone ablation is the therapy of choice for hormone-sensitive breast tumors, yet as many as 40% of patients inevitably relapse, and these hormone refractory tumors often have a poor prognosis. Epigenetic studies could provide DNA methylation biomarkers to predict and diagnose acquired resistance in response to treatment. Elucidation of epigenetic mechanisms may also lead to the development of new treatments that specifically target epigenetic abnormalities or vulnerabilities in cancer cells. Expectations must be tempered by the fact that epigenetic mechanisms of endocrine resistance remain poorly understood, and further study is required to better understand how altering epigenetic pathways with therapeutics can promote or inhibit endocrine resistance in different contexts. Going forward, DNA methylome profiling will become increasingly central to epigenetic research, heralding a network-based approach to epigenetics that promises to advance our understanding of the etiology of cancer in ways not previously possible.