Two-photon excitation fuorescence lifetime imaging microscopy:A promising diagnostic tool for digestive tract tumors

Digestive tract tumors acount for 15%and 19.3%of the cancer incidence and deaths,respec-tively.Early detection of digestive tract tumors is crucial to the reduction of global cancer burden.Two-photon excitation fuorescence lifetime imaging microscopy(TP-FLIM)allows non-invasive,label free,three-dimensional,high-resolution imaging of living tisues with not only histological but also biochemical characterization ability in both qualitative and quantitative way.Benefiting from these advantages,this technology is protmising for clinical diagnosis of digestive tract tumors.In recent years,many efforts have'been made in this field and some remarkable progress has been achieved.In this paper,we overview the recent progress of TP-FLIM-based researches on digestive tract tumor detection.Among them,our latest results on the gastric cancer and esophageal cancer are elaborately depicted.Finally,we outlook and discuss the potential advantages and challenges of TP-FLIM in future clinical applications.

Layered-resolved autofluorescence imaging of photo-receptors using two-photon excitation

In this paper, we present our investigation on the morphological and autofluorescence char-acteristics of the cones and rods using two- photon excitation with a femtosecond Ti: sap-phire laser. The results show that the micro-structures of the photoreceptor layers can be visualized at submicron level without any stain- ing or slicing. The morphology and spatial dis-tribution of the cones and rods can be resolved by autofluorescence imaging. The autofluores-cence in the photoreceptor outer segments is much stronger than other layers, but suscepti-ble to light-induced damage.

Two-photon excitation for C_2Vmolecules based on the full relativistic theory

The present work explores a new phenomenon that not all the transition probability of two photon processes is negligible at low irradiance. The irreducible representation 2B2 of C2v is unexpected, for there is no much deviation in oscillator strength for two-photon and single-photon process A1 to 2B2. This new phenomenon is only possible to be explored by the symmetrical consideration: the necessary and sufficient condition is molecular plane coincident with yz plane or the operation σ ’v(yz) for group C2v. It is only possible to be evaluated out by use of the full relativistic quantum mechanical theory.

Ultrafast carrier dynamics of Cu_(2)O thin film induced by two-photon excitation

Cuprous oxide(Cu_(2)O)has attracted plenty of attention for potential nonlinear photonic applications due to its superior third-order nonlinear optical property such as two-photon absorption.In this paper,we investigated the two-photon excitation induced carrier dynamics of a Cu_(2)O thin film prepared by radio-frequency magnetron sputtering,using the femtosecond transient absorption experiments.Biexponential dynamics including an ultrafast carrier scattering(<1 ps)followed by a carrier recombination(>50 ps)were observed.The time constant of carrier scattering under two-photon excitation is larger than that under one-photon excitation,due to the different transition selection rules and smaller absorption coefficient of the two-photon excitation.

Tuning Excitation Transport in a Dissipative Rydberg Ring

We demonstrate the flexible tunability of excitation transport in Rydberg atoms,under the interplay of controlled dissipation and interaction-induced synthetic flux.Considering a minimum four-site setup,i.e.,a triangular configuration with an additional output site,we study the transport of a single excitation.

Twin-Capture Rydberg State Excitation Enhanced with Few-Cycle Laser Pulses

Quantum excitation is usually regarded as a transient process occurring instantaneously,leaving the underlying physics shrouded in mystery.Recent research shows that Rydberg-state excitation with ultrashort laser pulses can be investigated and manipulated with state-of-the-art few-cycle pulses.We theoretically find that the efficiency of Rydberg state excitation can be enhanced with a short laser pulse and modulated by varying the laser intensities.We also uncover new facets of the excitation dynamics,including the launching of an electron wave packet through strong-field ionization,the re-entry of the electron into the atomic potential and the crucial step where the electron makes a U-turn,resulting in twin captures into Rydberg orbitals.By tuning the laser intensity,we show that the excitation of the Rydberg state can be coherently controlled on a sub-optical-cycle timescale.Our work paves the way toward ultrafast control and coherent manipulation of Rydberg states,thus benefiting Rydberg-state-based quantum technology.

Low-Energy Spin Excitations in Detwinned FeSe

Antiferromagnetic spin fluctuation is regarded as the leading driving force for electron pairing in high-Tc superconductors.In iron-based superconductors,spin excitations at low energy range,especially the spin-resonance mode at ER~5kBTc,are important for understanding the superconductivity.Here,we use inelastic neutron scattering(INS)to investigate the symmetry and in-plane wave-vector dependence of low-energy spin excitations in uniaxial-strain detwinned Fe Se.The low-energy spin excitations(E<10 meV)appear mainly at Q=(±1,0)in the superconducting state(T9K)and the nematic state(T90 K),confirming the constant C_(2) rotational symmetry and ruling out the C_(4) mode at E≈3 meV reported in a prior INS study.Moreover,our results reveal an isotropic spin resonance in the superconducting state,which is consistent with the s±wave pairing symmetry.At slightly higher energy,low-energy spin excitations become highly anisotropic.The full width at half maximum of spin excitations is elongated along the transverse direction.The Q-space isotropic spin resonance and highly anisotropic low-energy spin excitations could arise from dyz intra-orbital selective Fermi surface nesting between the hole pocket aroundΓpoint and the electron pockets centered at MX point.

Mapping the antiparallel aligned domain rotation by microwave excitation

The evolution process of magnetic domains in response to external fields is crucial for the modern understanding and application of spintronics.In this study,we investigated the domain rotation in stripe domain films of varying thicknesses by examining their response to microwave excitation in four different orientations.The resonance spectra indicate that the rotation field of stripe domain film under an applied magnetic field approaches the field where the resonance mode of sample changes.The saturation field of the stripe domain film corresponds to the field where the resonance mode disappears when measured in the stripe direction parallel to the microwave magnetic field.The results are reproducible and consistent with micromagnetic simulations,providing additional approaches and techniques for comprehending the microscopic mechanisms of magnetic domains and characterizing their rotation.

Low-energy inelastic electron scattering from carbon monoxide:Excitation and de-excitation of the X^(1)Σ^(+),a^(3)Π,a'^(3)∑^(+),A^(1)Π,d^(3)Δ,e^(3)∑^(-),I^(1)∑^(-)and D^(1)Δelectronic states

Cross-sections for electronic excitation and de-excitation among the ground state and lowest-lying seven electronic excited states of carbon monoxide(CO)by low-energy electron impact are computed using the R-matrix method.The excitation cross-sections from the ground state to the electronic states a^(3)Π,a'^(3)Σ^(+)+and A^(1)Πagree with previous experimental and theoretical results.In addition,the cross-sections for the I^(1)Σ^(+)-and D^(1)Δstates of CO,which will cascade to CO a'^(3)Σ^(+)+and A^(1)Πstates,are calculated.Furthermore,in contrast to the typical increase in electronic excitation cross-sections with collision energy,the de-excitation cross-sections show a negative trend with increasing energy.

Excitation and ionization of OCS molecules in strong UV and NIR laser fields

Rydberg state excitation(RSE) is a highly non-linear physical phenomenon that is induced by the ionization of atoms or molecules in strong femtosecond laser fields. Here we observe that both parent and fragments(S, C, OC) of the triatomic molecule carbonyl sulfide(OCS) can survive strong 800 nm or 400 nm laser fields in high Rydberg states. The dependence of parent and fragment RSE yields on laser intensity and ellipticity is investigated in both laser fields, and the results are compared with those for strong-field ionization. Distinctly different tendencies for laser intensity and ellipticity are observed for fragment RSE compared with the corresponding ions. The mechanisms of RSE and strong-field ionization of OCS molecules in different laser fields are discussed based on the experimental results. Our study sheds some light on the strong-field excitation and ionization of molecules irradiated by femtosecond NIR and UV laser fields.

A new method for deriving broad-band polar motion geodetic excitations

While the geodetic excitationχ(t)of polar motion p(t)is essential to improve our understanding of global mass redistributions and relative motions with respect to the terrestrial frame,the widely adopted method to deriveχ(t)from p(t)has biases in both amplitude and phase responses.This study has developed a new simple but more accurate method based on the combination of the frequency-and time-domain Liouville's equation(FTLE).The FTLE method has been validated not only with 6-h sampled synthetic excitation series but also with daily and 6-h sampled polar motion measurements as well asχ(t)produced by the interactive webpage tool of the International Earth Rotation and Reference Systems Service(IERS).Numerical comparisons demonstrate thatχ(t)derived from the FTLE method has superior performances in both the time and frequency domains with respect to that obtained from the widely adopted method or the IERS webpage tool,provided that the input p(t)series has a length around or more than 25 years,which presents no practical limitations since the necessary polar motion data are readily available.The FTLE code is provided in the form of Mat Lab function.

Core level excitation spectra of La and Mn ions in LaMnO3

Manganese-based perovskite is popular for research on ferromagnetic materials,and its spectroscopic studies are essential for understanding its electronic structure,dielectric,electrical,and magnetic properties.In this paper,the M-edge spectra of La ions and the M-edge,L-edge,and K-edge spectra of Mn ions in LaMnO3 are calculated by considering both the free-ion multiplet calculation and the crystal field effects.We analyze spectral shapes,identify peak origins,and estimate the oxidation states of La and Mn ions in LaMnO3 theoretically.It is concluded that La ions in LaMnO3 predominantly exist in the trivalent state,while Mn ions exist primarily in the trivalent state with a minor presence of tetravalent ions.Furthermore,the calculated spectra are in better conformity with the experimental spectra when the proportion of Mn3+is 90%and Mn4+is 10%.This article enhances our comprehension of the oxidation states of La and Mn within the crystal and also provides a valuable guidance for spectroscopic investigations of other manganates.

Vibration attenuation performance of wind turbine tower using a prestressed tuned mass damper under seismic excitation

With the rapid development of large megawatt wind turbines,the operation environment of wind turbine towers(WTTs)has become increasingly complex.In particular,seismic excitation can create a resonance response and cause excessive vibration of the WTT.To investigate the vibration attenuation performance of the WTT under seismic excitations,a novel passive vibration control device,called a prestressed tuned mass damper(PS-TMD),is presented in this study.First,a mathematical model is established based on structural dynamics under seismic excitation.Then,the mathematical analytical expression of the dynamic coefficient is deduced,and the parameter design method is obtained by system tuning optimization.Next,based on a theoretical analysis and parameter design,the numerical results showed that the PS-TMD was able to effectively mitigate the resonance under the harmonic basal acceleration.Finally,the time-history analysis method is used to verify the effectiveness of the traditional pendulum tuned mass damper(PTMD)and the novel PS-TMD device,and the results indicate that the vibration attenuation performance of the PS-TMD is better than the PTMD.In addition,the PS-TMD avoids the nonlinear effect due to the large oscillation angle,and has the potential to dissipate hysteretic energy under seismic excitation.

Auto-parametric resonance of a continuous-beam-bridge model under two-point periodic excitation:an experimental investigation and stability analysis

The auto-parametric resonance of a continuous-beam bridge model subjected to a two-point periodic excitation is experimentally and numerically investigated in this study.An auto-parametric resonance experiment of the test model is conducted to observe and measure the auto-parametric resonance of a continuous beam under a two-point excitation on columns.The parametric vibration equation is established for the test model using the finite-element method.The auto-parametric resonance stability of the structure is analyzed by using Newmark's method and the energy-growth exponent method.The effects of the phase difference of the two-point excitation on the stability boundaries of auto-parametric resonance are studied for the test model.Compared with the experiment,the numerical instability predictions of auto-parametric resonance are consistent with the test phenomena,and the numerical stability boundaries of auto-parametric resonance agree with the experimental ones.For a continuous beam bridge,when the ratio of multipoint excitation frequency(applied to the columns)to natural frequency of the continuous girder is approximately equal to 2,the continuous beam may undergo a strong auto-parametric resonance.Combined with the present experiment and analysis,a hypothesis of Volgograd Bridge's serpentine vibration is discussed.

Electron capture and excitation in intermediate-energy He^(2+)–H(1s,2s)collisions

The semiclassical non-perturbative atomic orbital close-coupling approach has been employed to study the electron capture and excitation processes in He^(2+)-H(1s)and He^(2+)-H(2s)collision systems.In order to ensure the accuracy of our calculated cross sections,a large number of high excited states and pseudostates are included in the expansion basis sets which are centered on the target and projectile,respectively.The total and partial charge transfer and excitation cross sections are obtained for a wide-energy domain ranging from 1 keV/amu to 200 keV/amu.The present calculations are also compared with the results from other theoretical methods.These cross section data are useful for the investigation of astrophysics and laboratory plasma.

Designing an Experimental Device for Swinging Excitation Spray Cooling

In this paper,we introduce the design principle of the oscillating excited spray cooling experimental device.We then designed an oscillating excited spray cooling experimental device.By using the device,the swaying motion can be realized through the control system,and the motion of the droplet under different vibration frequencies can be observed.By measuring the liquid flow rate and pressure,the changes in liquid flow rate,pressure,and temperature with time under different vibration frequencies were studied.The trajectory of the droplet and the temperature distribution of the droplet under different vibration frequencies could be observed.The device has a simple structure,is easy to control,and can achieve continuous observation of the spray cooling process.

uorescence microendoscopy imaging based on GRIN lenses with one- and two-photon excitation modes

With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in traditional fluorescence microscopes have limited their imaging applications mostly to superficial tissues. To overcome these disadvantages, researchers have developed the graded-index （GRIN） probes with small diameters for imaging internal organs of small animals in a minimally invasive fashion. However, dynamic imaging based on GRIN lens has not been studied extensively. Here, this paper presented a fluorescence endoscopic imaging system based on GRiN lenses using one-photon and two-photon excitation. GRIN lenses with 1.15 mm diameter and 7.65 mm length were used in the system. The images were acquired by a compact laser scanning imaging system with a resonant galvo-mirror system to scan the laser beam and a photomultiplier tube （PMT） to detect fluorescence signals. Experimental results showed that this system using two-photon excitation could implement dynamic fluorescence microendoscopic imaging and monitor the movement of blood flow beneath the skin in anesthetized mice while producing images with higher contrast and signal to noise ratio （SNR） than those using one photon excitation. It would be a useful tool for studying biological processes of small animals or plants in vivo.

A novel way to study the nuclear collective excitations

Typically, the unambiguous determination of the quantum numbers of nuclear states is a challenging task. Recently, it has been proposed to utilize to this aim vortex photons in the MeV energy region and, potentially, this could revolutionize nuclear spectroscopy because of the new and enhanced selectivity of this probe. Moreover, nuclei may become diagnostic tools for vortex photons. Still, some open questions have to be dealt with.Nuclei exhibit intricate excitation spectra. Indeed, not all states within these spectra are equally significant. Some are not sensitive to specific terms in the nuclear Hamiltonian or do not display novel features, so that investigating them is not helpful to enhance our overall understanding of nuclear structure. On the other hand, there are states that manifest themselves as prominent peaks, e.g., in the inelastic scattering spectra. Among the best examples are the so-called Giant Resonances that lie at energies of the order of tens of MeV [1].

Nonlinear Dynamics of Viscoelastic Pipe Conveying Pulsating Fluid Subjected to Base Excitation

Based on the Euler-Bernoulli beam theory and Kelvin-Voigt model,a nonlinear model for the transverse vibration of a pipe under the combined action of base motion and pulsating internal flow is established.The governing partial differential equation is transformed into a nonlinear system of fourth-order ordinary differential equations by using the generalized integral transform technique(GITT).The effects of the combined excitation of base motion and pulsating internal flow on the nonlinear dynamic behavior of the pipe are investigated using a bifurcation diagram,phase trajectory diagram,power spectrum diagram,time-domain diagram,and Poincare map.The results show that the base excitation amplitude and frequency significantly affect the dynamic behavior of the pipe system.Some new resonance phenomena can be observed,such as the period-1 motion under the base excitation or the pulsating internal flow alone becomes the multi-periodic motion,quasi-periodic motion or even chaotic motion due to the combined excitation action.

Method for Evaluating Bolt Competitive Failure Life Under Composite Excitation

In this study,the competitive failure mechanism of bolt loosening and fatigue is elucidated via competitive failure tests on bolts under composite excitation.Based on the competitive failure mechanism,the mode prediction model and“load ratio-life prediction curve”(ξ-N curve)of the bolt competitive failure are established.Given the poor correlation of theξ-N curve,an evaluation model of the bolt competitive failure life is proposed based on Miner’s linear damage accumulation theory.Based on the force analysis of the thread surface and simulation of the bolt connection under composite excitation,a theoretical equation of the bolt competitive failure life is established to validate the model for evaluating the bolt competitive failure life.The results reveal that the proposed model can accurately predict the competitive failure life of bolts under composite excitation,and thereby,it can provide guidance to engineering applications.