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考虑CAV自主停车行为的混合交通均衡配流模型
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作者 韩飞 王子捷 +1 位作者 王建 孙超 《东南大学学报(自然科学版)》 EI CAS CSCD 北大核心 2024年第1期208-213,共6页
为了评估网联自动驾驶汽车(CAV)的自主停车行为对交通系统效率的影响,建立了CAV通勤流、人工驾驶汽车(HDV)通勤流、CAV自主停车流3类交通流混行下的交通均衡配流模型.通过引入CAV停车需求内生变量,考虑CAV对路段通行能力的提升效应,从... 为了评估网联自动驾驶汽车(CAV)的自主停车行为对交通系统效率的影响,建立了CAV通勤流、人工驾驶汽车(HDV)通勤流、CAV自主停车流3类交通流混行下的交通均衡配流模型.通过引入CAV停车需求内生变量,考虑CAV对路段通行能力的提升效应,从而定量描述CAV停车需求分布以及3类交通流在路段上混行的拥挤效应.分别采用用户均衡、随机用户均衡原则描述CAV、HDV出行者的路径选择行为,采用Logit离散选择模型描述自主停车CAV的停车场选择行为,由此建立多用户混合交通均衡条件以及等价的变分不等式(VI)模型.由于模型中CAV自主停车需求为未知的内生变量,提出一种改进的相继加权平均法求解该模型.最后,通过算例验证了混合交通均衡配流模型及求解算法的有效性. 展开更多
关键词 智能交通 交通分配 自主停车行为 网联自动驾驶汽车 混合交通流
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Two-state energy model and experimental study of coal adsorb methane 被引量:2
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作者 Zeng-Chao FENG Dong ZHAO Zhi-Xiang LIU Yan-Qi WANG Hong-Qiang GOU 《Journal of Coal Science & Engineering(China)》 2013年第4期488-492,共5页
There are two states of methane existing in coal, free methane and adsorptive methane. The two states of methane exchanged with each other which need the energy exchange with outside. It is released heat when methane ... There are two states of methane existing in coal, free methane and adsorptive methane. The two states of methane exchanged with each other which need the energy exchange with outside. It is released heat when methane adsorption on coal, instead of absorbed heat. According to the gas molecules Boltzmann energy distribution, is obtained the equilibrium equations of the two states of methane in coal, as well as the heat of adsorption equation when exchanged into each other. At the same time, high temperature experiments of methane adsorption on coal have been certificated to the theoretical model. At last the experimental results presented that: the two-state energy model could be accurately described the distribution of the two states of methane in the coal; the adsorption heat is related to the initial equilibrium state of methane adsorption; the adsorption heats are different with different coal ranks. 展开更多
关键词 two-state energy model adsorption heat chemical potential experimental study
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Effective Two-State Model and NOON States for Double-Well Bose-Einstein Condensates in Strong-Interaction Regime 被引量:1
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作者 YANG Xiao-Xue WU Ying 《Communications in Theoretical Physics》 SCIE CAS CSCD 2009年第8期244-246,共3页
The model of double-well Bose-Einstein condensates in the strong-interaction regime is shown to reduce adiabatically to an effective two-state model describing the Rabi oscillations between the two atomic Fock states ... The model of double-well Bose-Einstein condensates in the strong-interaction regime is shown to reduce adiabatically to an effective two-state model describing the Rabi oscillations between the two atomic Fock states |N, 0〉 and [0, N〉, and the NOON states of arbitrary ultracold atoms can therefore be generated periodically from the initial state of either one of the Foek states. 展开更多
关键词 double-well Bose-Einstein condensates effective two-state model NOON states
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面向混合编队领航CAVs的博弈换道决策模型
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作者 卢洁楚 傅惠 《广东工业大学学报》 CAS 2024年第4期26-33,共8页
以CAVs为领航车的混合编队有助于实现更顺畅和安全的道路交通,但当前研究较少关注于具有多车道分布特征的混合编队形成问题。对此,本文提出了一种面向混合编队领航CAVs的博弈换道决策模型。该模型建立数学优化与博弈论相结合的换道决策... 以CAVs为领航车的混合编队有助于实现更顺畅和安全的道路交通,但当前研究较少关注于具有多车道分布特征的混合编队形成问题。对此,本文提出了一种面向混合编队领航CAVs的博弈换道决策模型。该模型建立数学优化与博弈论相结合的换道决策机制,建立以最小化CAVs换道次数为目标的领航CAVs目标车道初始化流程,并依据CAVs博弈换道收益,更新领航CAVs目标车道,实现混合编队多车道分布。同时,基于博弈论建立CAVs与HDVs非合作博弈矩阵,综合考虑换道效率和安全性,分别设计时间和安全收益函数,量化CAVs换道风险,并使用交通软件SUMO建立微观仿真。实验结果表明,与基准模型相比,本文提出的博弈换道策略在不同混合交通量下混合编队完成率维持在97%以上,每组领航CAVs换道时间平均缩短约40%,每组领航CAV换道次数保持较低水平。 展开更多
关键词 混合编队 领航cavs 博弈换道 微观仿真
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CAV1在消化道肿瘤中的研究进展
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作者 吴志航 唐明政 +3 位作者 李晓凤 荣耀 崔岩 潘海邦 《中国比较医学杂志》 CAS 北大核心 2024年第7期110-120,共11页
消化道肿瘤是目前全球最常见的癌症之一,包括食管癌、胃癌、肝癌、胰腺癌和结直肠癌等,其预后不佳,治疗方法仍需进一步改进。小窝蛋白-1(caveolin⁃1,CAV1)在消化道肿瘤中具有双重调节作用,既是肿瘤抑制因子又是致癌因子。CAV1对消化道... 消化道肿瘤是目前全球最常见的癌症之一,包括食管癌、胃癌、肝癌、胰腺癌和结直肠癌等,其预后不佳,治疗方法仍需进一步改进。小窝蛋白-1(caveolin⁃1,CAV1)在消化道肿瘤中具有双重调节作用,既是肿瘤抑制因子又是致癌因子。CAV1对消化道肿瘤的细胞增殖、侵袭、转移、血管生成和药物耐受性等方面具有重要作用,调节CAV1蛋白及其相关信号通路可能成为治疗消化道肿瘤的策略之一。因此,本综述就CAV1的结构、功能、表达调控以及调节消化道肿瘤上皮-间充质转换(epithelial⁃mesenchymal transition,EMT)及其耐药性等方面分析CAV1与消化道肿瘤的关系,以期为临床消化道肿瘤的诊疗提供新的思路。 展开更多
关键词 cav1 消化道肿瘤 EMT 耐药
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Large Signal Modulation Characteristics in the Transition Regime for Two-State Lasing Quantum Dot Lasers
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作者 吕尊仁 季海铭 +4 位作者 杨晓光 罗帅 高凤 许锋 杨涛 《Chinese Physics Letters》 SCIE CAS CSCD 2016年第12期63-67,共5页
Large-signal modulation capability, as an important performance indicator, is directly related to the high-speed optical communication technology involved. We experimentally and theoretically investigate the large-sig... Large-signal modulation capability, as an important performance indicator, is directly related to the high-speed optical communication technology involved. We experimentally and theoretically investigate the large-signal modulation characteristics of the simultaneous ground-state (GS) and the excited-state (ES) lasing in InAs/OaAs quantum dot laser diodes. The large-signal modulation capability of total light intensity in the transition regime from OS lasing to two-state lasing is unchanged as the bias-current increases. However, GS and ES large-signal eye diagrams show obvious variations during the transition. Relaxation oscillations and large-signal eye diagrams for OS, ES, and total light intensities are numerically simulated and analyzed in detail by using a rate-equation model. The -ndings show that a complementary relationship between the light intensities for OS and ES lasing exists in both the transition regime and the two-state lasing regime, leading to a much smaller overshooting power and a shorter settling time for the total light intensity. Therefore, the eye diagrams of GS or ES lasing are diffuse whereas those of total light intensity are constant as the bias-current increases in the transition regime. 展开更多
关键词 GS for Large Signal Modulation Characteristics in the Transition Regime for two-state Lasing Quantum Dot Lasers ES of in
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A Deep Two-State Gated Recurrent Unit for Particulate Matter (PM_(2.5)) Concentration Forecasting
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作者 Muhammad Zulqarnain Rozaida Ghazali +3 位作者 Habib Shah Lokman Hakim Ismail Abdullah Alsheddy Maqsood Mahmud 《Computers, Materials & Continua》 SCIE EI 2022年第5期3051-3068,共18页
Air pollution is a significant problem in modern societies since it has a serious impact on human health and the environment.Particulate Matter(PM_(2.5))is a type of air pollution that contains of interrupted elements... Air pollution is a significant problem in modern societies since it has a serious impact on human health and the environment.Particulate Matter(PM_(2.5))is a type of air pollution that contains of interrupted elements with a diameter less than or equal to 2.5 m.For risk assessment and epidemiological investigations,a better knowledge of the spatiotemporal variation of PM_(2.5) concentration in a constant space-time area is essential.Conventional spatiotemporal interpolation approaches commonly relying on robust presumption by limiting interpolation algorithms to those with explicit and basic mathematical expression,ignoring a plethora of hidden but crucial manipulating aspects.Many advanced deep learning approaches have been proposed to forecast Particulate Matter(PM_(2.5)).Recurrent neural network(RNN)is one of the popular deep learning architectures which is widely employed in PM_(2.5) concentration forecasting.In this research,we proposed a Two-State Gated Recurrent Unit(TS-GRU)for monitoring and estimating the PM_(2.5) concentration forecasting system.The proposed algorithm is capable of considering both spatial and temporal hidden affecting elements spontaneously.We tested our model using data from daily PM_(2.5) dimensions taken in the contactual southeast area of the United States in 2009.In the studies,three evaluation matrices were utilized to compare the overall performance of each algorithm:Mean Absolute Error(MAE),Root Mean Square Error(RMSE),and Mean Absolute Percentage Error(MAPE).The experimental results revealed that our proposed TS-GRU model outperformed compared to the other deep learning approaches in terms of forecasting performance. 展开更多
关键词 Deep learning PM_(2.5)forecasting air pollution two-state GRU
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CAV1和CAV2在头颈部鳞状细胞癌组织中的表达及其对生存、免疫的影响
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作者 曹欣然 樊建春 +2 位作者 王霞 刘训涛 张斌 《神经药理学报》 2024年第4期9-17,共9页
目的:基于生物信息学方法筛选出头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)的关键生物标记物CAV1和CAV2,并探究其在HNSCC中的表达情况以及临床预后价值。方法:本研究中,我们首先基于GEPIA数据库比较了caveolins及c... 目的:基于生物信息学方法筛选出头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)的关键生物标记物CAV1和CAV2,并探究其在HNSCC中的表达情况以及临床预后价值。方法:本研究中,我们首先基于GEPIA数据库比较了caveolins及cavins在HNSCC的差异表达情况,随后同时在Ualcan平台分析了caveolins及cavins家族中差异基因与临床表型的关系;比较与临床表型相关的差异基因与HNSCC生存的关系;此外还进行了CAV1与CAV2的富集分析及免疫浸润分析。结果:GEPIA数据库分析显示CAV1、CAV2、CAVIN2、CAVIN3等存在显著相关性,其中CAV1、CAV2与临床表型显著相关且能显著影响HNSCC患者生存预后。GSEA富集分析显示CAV1主要上调IL-17 signaling pathway、PI3K-Akt signaling pathway、NOD-like receptor signaling pathway;CAV2主要上调NOD-like receptor signaling pathway、PI3K-Akt signaling pathway。免疫浸润分析表明CAV1及CAV2能显著改变HNSCC肿瘤免疫微环境,与多种免疫细胞浸润存在相关性。结论:CAV1与CAV2在HNSCC中高表达,是与免疫侵袭相关的独立预后因素,可作为确定HNSCC患者的免疫侵袭相关预后的候选预后标志物。 展开更多
关键词 cav1 cav2 头颈部鳞状细胞癌 免疫
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Cav3.2 channel regulates cerebral ischemia/reperfusion injury:a promising target for intervention 被引量:2
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作者 Feibiao Dai Chengyun Hu +7 位作者 Xue Li Zhetao Zhang Hongtao Wang Wanjun Zhou Jiawu Wang Qingtian Geng Yongfei Dong Chaoliang Tang 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第11期2480-2487,共8页
Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type ... Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type calcium channels.T-type calcium channel blockers,such as pimozide and mibefradil,have been shown to prevent cerebral ischemia/reperfusion injury-induced brain injury.However,the role of Cav3.2 channels in cerebral ischemia/reperfusion injury remains unclear.Here,in vitro and in vivo models of cerebral ischemia/reperfusion injury were established using middle cerebral artery occlusion in mice and high glucose hypoxia/reoxygenation exposure in primary hippocampal neurons.The results showed that Cav3.2 expression was significantly upregulated in injured hippocampal tissue and primary hippocampal neurons.We further established a Cav3.2 gene-knockout mouse model of cerebral ischemia/reperfusion injury.Cav3.2 knockout markedly reduced infarct volume and brain water content,and alleviated neurological dysfunction after cerebral ischemia/reperfusion injury.Additionally,Cav3.2 knockout attenuated cerebral ischemia/reperfusion injury-induced oxidative stress,inflammatory response,and neuronal apoptosis.In the hippocampus of Cav3.2-knockout mice,calcineurin overexpression offset the beneficial effect of Cav3.2 knockout after cerebral ischemia/reperfusion injury.These findings suggest that the neuroprotective function of Cav3.2 knockout is mediated by calcineurin/nuclear factor of activated T cells 3 signaling.Findings from this study suggest that Cav3.2 could be a promising target for treatment of cerebral ischemia/reperfusion injury. 展开更多
关键词 CALCINEURIN cav3.2 channel cerebral ischemia/reperfusion hippocampus HYPOXIA/REOXYGENATION inflammatory response nuclear factor of activated T cells 3 oxidative stress primary hippocampal neurons stroke
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Measurement Problem and Two-State Vector Formalism
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作者 Kunihisa Morita 《Journal of Modern Physics》 2015年第13期1864-1867,共4页
In this paper, I show that an interpretation of quantum mechanics using two-state vector formalism proposed by Aharonov, Bergmann, and Lebowitz, can solve one of the measurement problems formulated by Maudlin. Accordi... In this paper, I show that an interpretation of quantum mechanics using two-state vector formalism proposed by Aharonov, Bergmann, and Lebowitz, can solve one of the measurement problems formulated by Maudlin. According to this interpretation, we can simultaneously insist that the wave function of a system is complete, that the wave function is determined by the Schr?dinger equation, and that the measurement of a physical quantity always has determinate outcomes, although Maudlin in his formulation of the measurement problem states that these three claims are mutually inconsistent. Further, I show that my interpretation does not contradict the uncertainty relation and the no-go theorem. 展开更多
关键词 two-state VECTOR FORMALISM Measurement Problem No-Go THEOREM
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Einstein Dilemma and Two-State Vector Formalism
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作者 Kunihisa Morita 《Journal of Quantum Information Science》 2015年第2期41-46,共6页
In the famous EPR paper published in 1935, Einstein, Podolsky, and Rosen suggested a thought experiment, which later became known as the “EPR experiment”. Using the EPR experiment, they posited that quantum mechanic... In the famous EPR paper published in 1935, Einstein, Podolsky, and Rosen suggested a thought experiment, which later became known as the “EPR experiment”. Using the EPR experiment, they posited that quantum mechanics was incomplete. Einstein, however, was dissatisfied with the EPR paper and published a second work on the EPR experiment, in which he discussed the dilemma of choosing whether quantum mechanics was incomplete or nonlocal. Currently, most physicists choose the nonlocality of quantum mechanics over Einstein’s choice of the incompleteness of quantum mechanics. However, with an appropriate alternate hypothesis, both of these choices can be rejected. Herein, I demonstrate an approach to overcome the Einstein Dilemma by proposing a new interpretation invoked by a new formalism of quantum mechanics known as two-state vector formalism. 展开更多
关键词 two-state Vector FORMALISM EINSTEIN DILEMMA COMPLETENESS LOCALITY
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Transfer Matrix Approach for Two-State Scattering Problem with Arbitrary Coupling
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作者 Diwaker Aniruddha Chakraborty 《Chinese Physics Letters》 SCIE CAS CSCD 2015年第7期5-8,共4页
The present work deals with the calculation of transition probability between two diabatic potentials coupled by any arbitrary coupling. The method presented in this work is applicable to any type of coupling while fo... The present work deals with the calculation of transition probability between two diabatic potentials coupled by any arbitrary coupling. The method presented in this work is applicable to any type of coupling while for numerical calculations we have assumed the arbitrary coupling as Gaussian coupling. This arbitrary coupling is expressed as a collection of Dirac delta functions and by the use of the transfer matrix technique the transition probability from one diabatic potential to another diabatic potential is calculated. We examine our approach by considering the case of two constant potentials coupled by Gaussian coupling as an arbitrary coupling. 展开更多
关键词 Transfer Matrix Approach for two-state Scattering Problem with Arbitrary Coupling
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Theoretical Investigation for Two-state Reactivity of CO_2 Hydrogenation Catalyzed by Ru in Gas Phase
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作者 王永成 贾义明 +1 位作者 王文雪 马盼盼 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2016年第12期1819-1828,共10页
Gas-phase CO_2 catalyzed activation hydrogenation by Ru atoms was studied with density functional theory. Based on the structure optimization of different potential energy surfaces,there are two crossing points betwee... Gas-phase CO_2 catalyzed activation hydrogenation by Ru atoms was studied with density functional theory. Based on the structure optimization of different potential energy surfaces,there are two crossing points between singlet and triplet potential energy surfaces and there is a crossing point between quintet and triplet potential energy surfaces in the whole catalytic cycle. Spin transition probabilities in the vicinity of the intersections have been calculated by the Landau-Zener model theory. There are three minimum energy crossing points(MECPs) with strong spin-orbital coupling effect and higher spin transition probability,and all spin inversion occurred in s orbital and different d orbitals of ruthenium,indicating this is a typical two-state reactivity(TSR) reaction. Finally,the lowest energy reaction path is ensured. 展开更多
关键词 two-state reactivity(TSR) carbon dioxide hydrogenation minimum energy crossing point(MECP) transition metal catalyzed intersystem crossing(ISC)
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CAV1在肿瘤化疗敏感性和耐药性中的研究进展
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作者 张伟林 吴亚东 《现代肿瘤医学》 CAS 2024年第14期2674-2680,共7页
陷窝蛋白(caveolin-1,CAV1)是细胞膜上陷窝的基本成分之一,是一种完整的膜蛋白,与CAV1相关的信号转导可调节细胞增殖、侵袭、细胞死亡和脂质代谢,并与多种免疫相关信号有关。化疗是临床上治疗肿瘤、预防肿瘤复发的重要手段,但其在治疗... 陷窝蛋白(caveolin-1,CAV1)是细胞膜上陷窝的基本成分之一,是一种完整的膜蛋白,与CAV1相关的信号转导可调节细胞增殖、侵袭、细胞死亡和脂质代谢,并与多种免疫相关信号有关。化疗是临床上治疗肿瘤、预防肿瘤复发的重要手段,但其在治疗过程中时常发生药物耐药及敏感性降低,这在很大程度上削弱了化疗的治疗效果,而CAV1与肿瘤化疗的耐药性及敏感性关系密切。该文围绕相关机制及研究进展进行综述,并对这一领域未来的发展方向进行讨论,以期为基于肿瘤化疗治疗的临床试验及机制研究提供帮助。 展开更多
关键词 cav1 肿瘤 化疗敏感性 化疗耐药性
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雏鸡混合感染IBDV与CAV的研究 被引量:15
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作者 王笑梅 王秀荣 +1 位作者 邱冬 王积海 《中国预防兽医学报》 CAS CSCD 2000年第5期321-324,共4页
实验分为 4组 :空白对照组、CAV感染组、IBDV感染组、IBDV_CAV混合感染组。选择IBDV发病严重期 (6日龄 )与CAV发病严重期 (13日龄 )测定并计算各组鸡的法氏囊、胸腺和体重的比值 ;分离外周血、法氏囊和胸腺中的淋巴细胞 ,加入适量的刀... 实验分为 4组 :空白对照组、CAV感染组、IBDV感染组、IBDV_CAV混合感染组。选择IBDV发病严重期 (6日龄 )与CAV发病严重期 (13日龄 )测定并计算各组鸡的法氏囊、胸腺和体重的比值 ;分离外周血、法氏囊和胸腺中的淋巴细胞 ,加入适量的刀豆蛋白 (ConA)和商陆凝集素 (PAM)作为有丝分裂刺激原 ,利用MTT法检测实验鸡免疫细胞的功能变化 ;统计死亡率 ,绘制死亡曲线。研究发现 ,IBDV与CAV的混合感染较单独感染严重降低了感染鸡免疫活性细胞的增殖功能 ,并同时增强了IBDV或CAV的致病力 ,出现两次死亡高峰 ,病死率达 10 0 %。 展开更多
关键词 IBDV cav 混合感染
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传染性法氏囊病病料中MDV、CAV、REV的共感染检测 被引量:78
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作者 金文杰 崔治中 +1 位作者 刘岳龙 秦爱建 《中国兽医学报》 CAS CSCD 北大核心 2001年第1期6-9,共4页
从江苏、山东、浙江、河南、上海、广西、云南等地收集根据临床表现及病理变化诊断为传染性法氏囊病( IBD)的病鸡法氏囊样品 66份 ,用相应的核酸探针及斑点杂交法分别检测样品中鸡传染性贫血病病毒( CAV)、马立克氏病病毒 ( MDV)、网状... 从江苏、山东、浙江、河南、上海、广西、云南等地收集根据临床表现及病理变化诊断为传染性法氏囊病( IBD)的病鸡法氏囊样品 66份 ,用相应的核酸探针及斑点杂交法分别检测样品中鸡传染性贫血病病毒( CAV)、马立克氏病病毒 ( MDV)、网状内皮细胞增生病病毒 ( REV)的感染 ;用抗 IBD血清做琼扩检测 IB-DV。结果显示 ,部分地区病料中这些病毒有不同程度的二重感染、三重感染甚至四重感染。提示在研究特定毒株 IBDV的毒力时 ,应考虑多重感染对致病性的影响。 展开更多
关键词 传染性法氏囊病病毒 马立克氏病病毒 REV cav 共感染 鸡群
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CAV与REV共感染SPF鸡对疫苗免疫反应的抑制作用 被引量:23
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作者 李延鹏 崔治中 +1 位作者 姜世金 郭慧君 《中国兽医学报》 CAS CSCD 北大核心 2008年第11期1243-1246,共4页
用1日龄SPF鸡人工感染鸡贫血病毒(CAV)和禽网状内皮增生病病毒(REV),探讨病毒感染对鸡体疫苗免疫反应的影响。结果表明,在用禽流感病毒(AIV,H5和H9)疫苗免疫后,CAV与REV单独感染均显著抑制了鸡体对H5和H9亚型禽流感病毒灭活疫苗的HI抗... 用1日龄SPF鸡人工感染鸡贫血病毒(CAV)和禽网状内皮增生病病毒(REV),探讨病毒感染对鸡体疫苗免疫反应的影响。结果表明,在用禽流感病毒(AIV,H5和H9)疫苗免疫后,CAV与REV单独感染均显著抑制了鸡体对H5和H9亚型禽流感病毒灭活疫苗的HI抗体反应,在CAV与REV共感染后,这种抑制作用更为明显。CAV单独感染后鸡体对新城疫病毒(NDV)和传染性法氏囊病病毒(IBDV)疫苗的免疫反应受到抑制,但与对照组在统计学上的差异不显著,然而,CAV可以显著加重REV感染对鸡体在NDV和IBDV疫苗免疫后抗体反应的抑制作用。从而证实CAV与REV共感染在疫苗免疫抑制上有协同作用。 展开更多
关键词 鸡贫血病毒(cav) 禽网状内皮增生病病毒(REV) 免疫抑制 共感染 协同作用
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体外重组CaV1.2不同蛋白片段纯化及其与CaM相互作用的研究 被引量:4
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作者 何桂林 邵冬雪 +4 位作者 印丹丹 胡慧媛 郭凤 王红梅 郝丽英 《中国医科大学学报》 CAS CSCD 北大核心 2013年第9期773-776,共4页
目的纯化CaV1.2不同蛋白片段并研究其与CaM的相互作用。方法质粒转化、筛选得到转化成功BL21菌株,利用IPTG诱导GST钙通道融合蛋白表达,Glutathione-Sepharose 4B beads纯化钙通道不同蛋白片段;采用GST pull-down assay实验研究钙通道蛋... 目的纯化CaV1.2不同蛋白片段并研究其与CaM的相互作用。方法质粒转化、筛选得到转化成功BL21菌株,利用IPTG诱导GST钙通道融合蛋白表达,Glutathione-Sepharose 4B beads纯化钙通道不同蛋白片段;采用GST pull-down assay实验研究钙通道蛋白不同片段与CaM以及突变体的相互作用。结果 SDS-PAGE结果显示成功纯化获得钙通道不同蛋白片段;GST pull-down assay结果显示CT1可与CaM及突变体结合,且GST-CT1与野生型CaM结合量显著多于与突变体CaM结合量。结论成功纯化CaV1.2钙通道蛋白片段并顺利完成GST pull-down assay实验,为深入研究蛋白相互作用或发现新的蛋白相互作用奠定了基础。 展开更多
关键词 GSTpull-downassay cav1.2 CAM 融合蛋白
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累积绝对速度CAV和峰值加速度PGA联合报警在高铁地震监测预警中的应用 被引量:6
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作者 黄俊 姚运生 +1 位作者 陈志高 杨江 《灾害学》 CSCD 北大核心 2014年第2期213-216,共4页
美国电力研究所EPRI在1991年通过研究认为累积绝对速度CAV对低频震动敏感而对高频震动不敏感,能很好避免没有破坏性的高频小震报警。简要介绍CAV的概念和标准化算法,并以汶川地震及部分余震数据为例,分析高铁采用CAV参数报警的可行性,... 美国电力研究所EPRI在1991年通过研究认为累积绝对速度CAV对低频震动敏感而对高频震动不敏感,能很好避免没有破坏性的高频小震报警。简要介绍CAV的概念和标准化算法,并以汶川地震及部分余震数据为例,分析高铁采用CAV参数报警的可行性,结果表明高铁地震监测预警中CAV参数可能会对破坏性小的远震大震报警,但CAV参数和峰值加速度PGA参数联合报警能有效地排除近震小震和远震大震对高铁地震报警的干扰。 展开更多
关键词 高铁地震监测预警 报警参数 标准化cav算法 峰值加速度
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传统CAV方案与金喜素联合顺铂治疗小细胞肺癌疗效回顾对比分析 被引量:2
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作者 傅恩清 金发光 +5 位作者 孙亚妮 刘春丽 刘静莉 楚东岭 刘同刚 谢永宏 《现代生物医学进展》 CAS 2006年第11期58-59,共2页
目的:分组比较传统CAV方案(环磷酰胺+阿霉素+长春新硷)和金喜素和顺铂方案对小细胞肺癌患者的治疗效果。方法:通过分组对比分析164例小细胞肺癌患者连续三次应用传统CAV(环磷酰胺、阿霉素、长春新硷)方案和连续三次应用金喜素联合顺铂... 目的:分组比较传统CAV方案(环磷酰胺+阿霉素+长春新硷)和金喜素和顺铂方案对小细胞肺癌患者的治疗效果。方法:通过分组对比分析164例小细胞肺癌患者连续三次应用传统CAV(环磷酰胺、阿霉素、长春新硷)方案和连续三次应用金喜素联合顺铂方案化疗,检测肿块横径与直径缩小程度,比较两个方案对小细胞肺癌的治疗作用。结果:金喜素联合顺铂方案时小细胞肺癌总有效率迭56.5%,传统CAV方案总有效率41.8%,两组比较有明显统计学意义(x2=18.4>16.27,v=3,p<0.001),两组毒副反应比较无统计学意义(x2=5.62<9.49,v=4,p>0.05)。结论:金喜素联合顺铂方案时小细胞肺癌较传统CAV方案有更明显的疗效,值得临床应用。 展开更多
关键词 金喜素 顺铂 化疗 疗效 cav方案
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