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UPLC-MS/MS法测定生菜中吗啉胍含量及不确定度评价 被引量:1
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作者 李彤辉 袁利杰 +1 位作者 尚伟 张培毅 《粮油食品科技》 CAS CSCD 2023年第4期123-129,共7页
建立超高效液相色谱串联质谱法(ultra performance liquid chromatography-tandem massspectrometry,UPLC-MS/MS)测定生菜中吗啉胍残留量的分析方法并进行不确定度评价。样品经提取净化后,通过电喷雾离子源正离子扫描、多反应监测模式(M... 建立超高效液相色谱串联质谱法(ultra performance liquid chromatography-tandem massspectrometry,UPLC-MS/MS)测定生菜中吗啉胍残留量的分析方法并进行不确定度评价。样品经提取净化后,通过电喷雾离子源正离子扫描、多反应监测模式(MRM)对吗啉胍进行定量分析。通过建立数学模型,分析测量不确定度来源,并量化各不确定度分量,合成不确定度。吗啉胍在0.1~20 mg/L范围内呈良好线性,R^(2)为0.9993,在0.5、5.0和10.0μg/kg这3个水平的加标回收率为84.08%~98.06%(n=6),相对标准偏差为3.58%~5.26%(n=6)。样品中吗啉胍含量测定结果为17.5μg/kg时,在95%置信区间下,扩展不确定度为2.84μg/kg(k=2)。该方法前处理快速高效、准确度高、重现性好,回收率及精密度均能达到实验要求,不确定度主要由标准溶液、标准曲线的建立及样品加标回收引起。实验结果可为UPLC-MS/MS法测定生菜中吗啉胍残留量及不确定度评价提供参考。 展开更多
关键词 超高效液相色谱串联质谱法 不确定度 吗啉胍 生菜
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Sensitive and Rapid Quantification of Felodipine by High-performance Liquid Chromatography-tandem Mass Spectrometry(HPLC-MS/MS) and Its Pharmacokinetics in Healthy Chinese Volunteers 被引量:5
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作者 LI Yan-yan YIN Yi-zi +4 位作者 SUN Zhi-hui LI Xin HU Li-gang LI Peng-fei ZHONG Da-fang 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2006年第4期479-483,共5页
To investigate the pharmacokinetics of felodipine in the plasma of healthy Chinese volunteers, 30 healthy volunteers received a single oral dose of 5 mg of extended release felodipine tablets. The felodipine was extra... To investigate the pharmacokinetics of felodipine in the plasma of healthy Chinese volunteers, 30 healthy volunteers received a single oral dose of 5 mg of extended release felodipine tablets. The felodipine was extracted from the matrix with a liquid-liquid extract procedure and analyzed by high-performance liquid chromatography-tandem mass spectrometry in the multiple reaction monitoring(MRM) mode using an electrospray ion source with positive ion detection. The method was validated over a felodipine concentration range of 0. 05-10.00 ng/mL in human plasma. Its main pharmacokinetic parameters values were: ρmax = ( 1.67 ± 0. 84 ) ng/mL, occurring at ( 3.93 3± 2. 49 ) h; the plasma elimination half-life: (23. 08 3± 9. 48) h and the area under the plasma concentration versus time curve: (29. 94 ± 14. 39) ng · h/mL. The validation results demonstrated that this method showed a satisfactory precision and accuracy across the calibration range. The procedure involved minimal drug administration, sample preparation, and a 2. 5-min chromatographic run time. It was well suited to clinical studies of the drug involving large numbers of samples. 展开更多
关键词 FELODIPINE liquid chromatography-tandem massspectrometry PHARMACOKINETICS
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Liquid chromatography–tandem mass spectrometry method for simultaneous determination of valproic acid and its ene-metabolites in epilepsy patient plasma 被引量:2
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作者 Huan Lu Chong Su +3 位作者 Lei Yin Liqiang Gu Jingkai Gu Xiaohui Chen 《Journal of Pharmaceutical Analysis》 SCIE CAS 2016年第2期112-116,共5页
A simple and high throughput method was developed and validated for simultaneous determination of valproic acid and its two toxicant ene-metabolites, 2-enevalproic acid and 4-enevalproic acid in epilepsy patient plasm... A simple and high throughput method was developed and validated for simultaneous determination of valproic acid and its two toxicant ene-metabolites, 2-enevalproic acid and 4-enevalproic acid in epilepsy patient plasma using liquid chromatography-tandem mass spectrometry. Probenecid was used as in- ternal standard and solid-phase extraction was selected for sample preparation. A chromatographic separation was performed on an Agilent Poroshell SB-C18 column (50 mm × 4.6 mm i.d., 2.7μm) by an optimized gradient elution at a flow rate of 0.9 mL/min. The total run time was 7 rain. Electrospray ionization was used in negative ion mode by multiple reaction monitoring of the precursor-to-product ion transitions at m/z 143.0→143.0 for valproic acid, m/z 140.9 →140.9 for 2-enevalproic acid and 4-enevalproic acid for their poor fragments, and m/z 283.9→239,9 for probenecid. The results showed good linearity ofvalproic acid, 2-enevalproic acid and 4-enevalproic acid in their respective linear ranges. The correlation coefficients were more than 0.998, The intra- and inter-day precision of the assay was less than 11.0% and the accuracy ranged from 2% to 12%. This analytical method was successfully applied to assay plasma concentrations of valproic acid and its two ene-metabolites in epilepsy patient plasma and used for therapeutic drug monitoring. 展开更多
关键词 liquid chromatography-tandem massspectrometry Valproic acid 2-enevalproic acid 4-enevalproic acid
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Liquid chromatography-tandem mass spectrometry method for the estimation of adefovir in human plasma:Application to a pharmacokinetic study
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作者 Dipanjan Goswami Sanjay Gurule +3 位作者 Arabinda Saha Poonam Vats Arshad Khuroo Tausif Monif 《Journal of Pharmaceutical Analysis》 SCIE CAS 2015年第3期190-199,共10页
An analytical method based on solid phase extraction was developed and validated for analysis of adefovir in human plasma. Adefovir-d4 was used as an internal standard and Synergi MAX RP80A (150rnmx4.6mm. 41am) colu... An analytical method based on solid phase extraction was developed and validated for analysis of adefovir in human plasma. Adefovir-d4 was used as an internal standard and Synergi MAX RP80A (150rnmx4.6mm. 41am) column provided the desired chromatographic separation of compounds followed by detection with mass spectrometry. The method used simple isocratic chromato- graphic condition and mass spectrometric detection in the positive ionization mode. The calibration cuives were linear over the range of 0.50-42.47 ng/mL with the lower limit of quantitation validated at 0.50 ng/mL. Matrix effect was assessed by post-column infusion experiment to monitor phospholipids and post- extraction addition experiment was performed. The degree of matrix effect for adefovir was determined as 7.5% and ion-enhancement in five different lots of human plasma was 7.1% and had no impact on study samples analysis with 4.5 rain run time. The intra- and inter-day precision values were within 7.7% and 7.8%, respectively, for adefovir at the lower limit of quantification level. Validated bioanalytical method was successfully applied to clinical sample analysis. 展开更多
关键词 liquid chromatography-tandem massspectrometry Solid phase extraction Pharmacokinetic study
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Simultaneous Determination of Six Compounds in Rat Plasma by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry: Application in the Pharmacokinetic Study of Qing Gan-Shu Yu-Fang 被引量:1
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作者 Hai Jiang A-Jiao Houa +10 位作者 Yan-Yan Zhang Wen-Jing Man Liu Yang Yong-Hai Meng Xin-Yue Guo Song Wang Jia-Xu Zhang Bing-You Yang Qiu-Hong Wang Kelvin Chan Hai-Xue Kuang 《World Journal of Traditional Chinese Medicine》 2019年第4期250-259,共10页
A rapid and high selective ultra-performance liquid chromatography(UPLC)with tandem mass spectrometry method for simultaneous determination of six compounds including albiflorin,paeoniflorin,picroside I,picroside II,s... A rapid and high selective ultra-performance liquid chromatography(UPLC)with tandem mass spectrometry method for simultaneous determination of six compounds including albiflorin,paeoniflorin,picroside I,picroside II,saikosaponin A,and saikosaponin D in rat plasma was developed and validated using butyl p-hydroxybenzoate as an internal standard.One-step direct protein precipitation with acetonitrile was used to extract the compounds from the rat plasma samples.Chromatographic separation was achieved using an ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7μm)at a flow rate of 0.4 m L/min,using gradient mode containing 0.1%formic acid in water and acetonitrile were used as the Mobile phase A and B.Electrospray ionization in negative ion mode and multiple reaction monitoring were used to identify and quantify active components.Calibration curves showed good linearity(R^2>0.9908)over a wide concentration range for all compounds.The intra-and interday precision(relative standard deviation)ranged 2.4%–7.0%and 2.6%–8.0%,respectively.The accuracy(relative error)was from-13.0%to 13.2%at all quality control levels.The recovery ranged from 81.1%to 92.5%.The validated method was successfully applied to pharmacokinetic study in rats after oral administration of Qing Gan-Shu Yu-Fang.The results show that one can draw a conclusion that these six active ingredients can be quickly absorbed and play a pharmacodynamic role rapidly in vivo. 展开更多
关键词 PHARMACOKINETICS traditional Chinese medicine prescriptions ultra-performance liquid chromatography with tandem mass spectrometry
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An Automatic Solid Phase Extraction and Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry for Determination of Seven Microcystins at Ultra-Trace Levels in Surface Water
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作者 SHEN Fei XU Yanjuan +1 位作者 WANG Ye CHEN Jing 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2019年第1期30-36,共7页
A method was developed for the detection of seven microcystins(microcystin-LR, RR, YR, LA, LY, LW and LF) in surface water using automatic solid-phase extraction(A-SPE) coupled with ultra-performance liquid chromatogr... A method was developed for the detection of seven microcystins(microcystin-LR, RR, YR, LA, LY, LW and LF) in surface water using automatic solid-phase extraction(A-SPE) coupled with ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS). The automated solid-phase extraction system was used to extract microcystins(MCs) from water samples. UPLC-MS/MS was used to determine MCs concentrations in just 5 min. Method detection limits were from 0.3 to 0.9 ng/L, microcystin recoveries ranged from 83.8% to 114%, and the relative standard deviation(RSD) varied from 5.6% to 12.5%. This analytical approach was found to be simple, highly sensitive, accurate, which required little manual operation. Additionally, to validate this analytical method, A-SPE+UPLC-MS/MS was applied to characterize the concentration of MCs in Taihu Lake, Wuxi, China. 展开更多
关键词 MICROCYSTINS AUTOMATIC SOLID-PHASE extraction ultra-performance liquid chromatography tandem mass spectrometry(uplc-ms/ms)
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Banxia xiexin decoction prevents the development of gastric cancer
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作者 Guo-Xiu Zu Ke-Yun Sun +3 位作者 Xi-Jian Liu Ji-Qin Tang Hai-Liang Huang Tao Han 《World Journal of Clinical Oncology》 2024年第10期1293-1308,共16页
BACKGROUND In China banxia xiexin decoction(BXD)has been used in treating gastric cancer(GC)for thousands of years and BXD has a good role in reversing GC histopathology,but its chemical composition and action mechani... BACKGROUND In China banxia xiexin decoction(BXD)has been used in treating gastric cancer(GC)for thousands of years and BXD has a good role in reversing GC histopathology,but its chemical composition and action mechanism are still unknown.AIM To investigate the mechanism of action of BXD against GC based on transcriptomics,network pharmacology,in vivo and in vitro experiments.METHODS The transplanted tumor model was prepared,and the nude mouse were pathologically examined after administration,and hematoxylin-eosin staining was performed.The active ingredients of BXD were quality controlled and identified using ultra-performance liquid chromatography tandem quadrupole electrostatic field orbitrap mass spectrometry(UPLC-Q-Orbitrap MS/MS),and traditional Chinese medicines systems pharmacology platform,drug bank and the Swiss target prediction platform to predict the relevant targets,the differentially expressed genes(DEGs)of GC were screened by RNA-seq sequencing,and the overlapping targets were analyzed to obtain the key targets and pathways.Cell Counting Kit-8,apoptosis assay,cell migration and Realtime fluorescence quantitative polymerase chain reaction were used for in vitro experiments.RESULTS All dosing groups inhibited the growth of transplanted tumors in laboratory-bred strain nude,with the capecitabine group and the BXD medium-dose group being the best.A total of 29 compounds and 859 potential targets in BXD were identified by UPLC-Q-Orbitrap MS/MS and network pharmacology,RNA-seq sequencing found 4767 GC DEGs,which were combined with network pharmacology and analyzed 246 potential therapeutic targets were obtained and pathway results showed that BXD may against GC through the Phosphoinositide 3-kinase(PI3K)/protein kinase B(AKt)signaling pathway.In vitro cellular experiments confirmed that BXDcontaining serum and LY294002 could inhibit the proliferation of GC cells,promote apoptosis,and inhibit the migration of GC cells by decreasing the expression of EGFR,PIK3CA,IL6,BCL2 and AKT1 in the PI3K-Akt pathway in MGC-803 expression.CONCLUSION BXD has the effect of inhibiting tumor growth rate and delaying the development of GC.Its mechanism of action may be related to the regulation of PI3K-Akt signaling pathway. 展开更多
关键词 Banxia xiexin decoction Gastric cancer ultra-performance liquid chromatography tandem quadrupole elec-trostatic field orbitrap mass spectrometry Network pharmacology Whole transcriptomic sequencing Phosphoinositide 3-kinase/protein kinase B signaling pathway
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Determination of L-ergothioneine in food by UPLC-MS/MS method
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作者 Hongwen Zhai Lei Sun +5 位作者 Sufang Fan Jing Wang Meirong Cao Hongyu Ma Qiang Li Yan Zhang 《Journal of Future Foods》 2023年第2期163-168,共6页
L-Ergothioneine(L-EGT)possesses excellent antioxidant activity and has been used in the food,pharmaceuticals and cosmetics industries.In this study,a new efficient and sensitive ultra-performance liquid chromatography... L-Ergothioneine(L-EGT)possesses excellent antioxidant activity and has been used in the food,pharmaceuticals and cosmetics industries.In this study,a new efficient and sensitive ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method was established for the quantitative determination of L-EGT in food.The sample was extracted with methanol-water(70:30,V/V),separated by hydrophilic interaction liquid chromatography(HILIC)and detected by triple-quadrupole mass spectrometry.Validation studies were carried out on different product and the limit of quantitation was 20μg/kg(milk,alcohol-free beverages,dairy products)and 40µg/kg(cereal bars,chocolate).Excellent linearity(correlation coefficient(R2)≥0.999)was achieved for L-EGT quantification in the range of 5–200 ng/mL.The recoveries of the method(83.7%−107.5%)and the relative standard deviation(RSD,0.88%−6.84%(n=6))meet the performance criteria required for the determination of L-EGT in food.Finally,the applicability of the method was tested by analysing actual samples.In general,the method developed is simple,reliable,accurate,and stable and could be useful for routine analyses of L-EGT in food. 展开更多
关键词 L-Ergothioneine ultra-performance liquid chromatography tandem mass spectrometry Hydrophilic interaction liquid chromatography FOOD DETERMINATION
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Low temperature purification method for the determination of abamectin and ivermectin in edible oils by liquid chromatographytandem mass spectrometry 被引量:3
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作者 Jian-Xiang Huang Da-Hai Lu +1 位作者 Kai Wan Fu-Hua Wang 《Chinese Chemical Letters》 SCIE CAS CSCD 2014年第4期635-639,共5页
In this study, a method based on low temperature purification (LTP) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of abamectin (ABA) and ivermectin (... In this study, a method based on low temperature purification (LTP) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of abamectin (ABA) and ivermectin (IVR) in edible oils. ABA and IVR were extracted using conventional liquid-liquid extraction followed by purification via precipitation of interfering fatty components at low temperature without an additional cleanup step. LTP is simple, easy to use, labour-saving and cost effective, and requires reduced amounts of organic solvent. The linear ranges of ABA and IVR were 5- 1000 t^g/L using matrix-matched standards. Limits of detection (LOD) and limits of quantification (LOQ) were in the range of 0.1-0.4 i^g/kg and 0.3-1.3 p^g/kg, respectively. The LOQs were below the strictest maximum residue limits established by Codex Alimentarius Commission. Recoveries at three spiked levels of 10, 20 and 100 i^g/kg in peanut oil, corn oil, olive oil, soybean oil and lard ranged from 71.1% to 119.3% with relative standard deviations of 3.2%-10.3%, which were in agreement with those obtained by the solid phase extraction method. The proposed method was utilized in the analysis of 10 edible oil samples from local market and neither ABA nor IVR was detected. As far as we know, this is the first time that LTP is applied to the determination of avermectins in edible oils. 展开更多
关键词 Low temperature purification Abamectin Ivermectin Edible oil liquid chromatography-tandem massspectrometry
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超高效液相色谱-串联质谱测定动物源性食品和尿液中4种β-受体激动剂残留 被引量:46
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作者 王凤美 张鸿伟 +3 位作者 庞士平 汤志旭 牛增元 罗忻 《分析化学》 SCIE EI CAS CSCD 北大核心 2008年第12期1629-1635,共7页
建立了动物源性食品和尿液中4种β-受体激动剂克伦特罗、莱克多巴胺、沙丁胺醇和特布他林的超高效液相色谱-串联质谱测定方法。样品均质后,加入乙酸铵水溶液,采用β-葡萄糖醛酸酶/芳基硫酸酯酶酶解处理,过滤后,用Oasis MCX固相萃... 建立了动物源性食品和尿液中4种β-受体激动剂克伦特罗、莱克多巴胺、沙丁胺醇和特布他林的超高效液相色谱-串联质谱测定方法。样品均质后,加入乙酸铵水溶液,采用β-葡萄糖醛酸酶/芳基硫酸酯酶酶解处理,过滤后,用Oasis MCX固相萃取柱净化,采用UPLC-MS/MS多反应监测(MRM)模式检测,内标法定量。4种β-受体激动剂的检出限均为0.1μg/kg,定量下限为0.5μg/kg;在0.5、0.75和1.0μg/kg3个浓度添加水平,总体平均回收率为86.7%~114.3%,总体相对标准偏差均在10%以内。本方法分析速度快,灵敏度高,重现性好,各项技术指标均满足国内外相关法规要求,可用于各种动物源性食品及尿液中4种β-受体激动剂残留的快速检测。 展开更多
关键词 β-受体激动剂 动物源性食品 超高效液相色谱-串联质谱
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超高效液相色谱-串联质谱法同时测定饲料中3种氯丙那林异构体和巴氯芬 被引量:9
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作者 李阳 苏晓鸥 +3 位作者 王培龙 樊霞 王瑞国 王骁 《分析化学》 SCIE EI CAS CSCD 北大核心 2014年第4期525-530,共6页
以溶剂提取-固相萃取为前处理方式,建立了超高效液相色谱-串联质谱法检测饲料中3种氯丙那林异构体和巴氯芬的新方法.试样用0.1 mol/L HCl-甲醇(4∶1,V/V)溶剂振荡提取20 min,离心去杂质后由MCX柱净化、浓缩,经Waters BEH C18色谱柱梯... 以溶剂提取-固相萃取为前处理方式,建立了超高效液相色谱-串联质谱法检测饲料中3种氯丙那林异构体和巴氯芬的新方法.试样用0.1 mol/L HCl-甲醇(4∶1,V/V)溶剂振荡提取20 min,离心去杂质后由MCX柱净化、浓缩,经Waters BEH C18色谱柱梯度洗脱分离,串联质谱法测定,正离子扫描,并以外标法计算结果.方法检出限为20 μg/kg,定量限为50 μg/kg.4种目标化合物在3个添加水平上进行回收实验,回收率均在80%~110%之间;相对标准偏差均小于或等于10.2%. 展开更多
关键词 饲料 氯丙那林 异构体 巴氯芬 超高效液相色谱-串联质谱
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蟾毒灵白蛋白纳米粒与蟾毒灵在大鼠体内的药代动力学比较研究 被引量:7
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作者 张慧卿 殷子斐 +3 位作者 盛佳钰 姜珠倩 吴宝玉 苏永华 《中西医结合学报》 CAS 2012年第6期674-680,共7页
目的:建立高效液相-串联质谱法测定大鼠血浆中蟾毒灵的浓度,评价和比较蟾毒灵白蛋白纳米粒和蟾毒灵在大鼠体内的药代动力学特征。方法:30只大鼠随机分为6组,每组5只,尾静脉单次分别注射蟾毒灵纳米粒和蟾毒灵,均设高、中、低剂量组。分... 目的:建立高效液相-串联质谱法测定大鼠血浆中蟾毒灵的浓度,评价和比较蟾毒灵白蛋白纳米粒和蟾毒灵在大鼠体内的药代动力学特征。方法:30只大鼠随机分为6组,每组5只,尾静脉单次分别注射蟾毒灵纳米粒和蟾毒灵,均设高、中、低剂量组。分别于给药后1、5、8、10、15、20、30、45、60、120、180、300和480min从大鼠眼眶静脉丛取血,高效液相-串联质谱法测定不同时间点血浆中药物浓度,并测定药代动力学参数。结果:本实验所建立的高效液相-串联质谱法具有良好的线性、精密度和准确度。蟾毒灵纳米粒组的血浆药-时曲线下面积和平均滞留时间、半衰期分别是蟾毒灵组的1.19~1.81倍、2.12~3.61倍和2.17~2.94倍。结论:蟾毒灵白蛋白纳米粒可延长蟾毒灵在血中的存留时间,具有缓释作用。 展开更多
关键词 蟾毒灵 纳米粒子 药代动力学 色谱法 高压液相 串联质谱法 大鼠
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超高效液相色谱-四极杆串联飞行时间质谱分析黄酮类化合物及小毛茛茎叶的成分 被引量:18
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作者 池玉梅 李瑶 +2 位作者 张瑜 王琴霞 崔小兵 《色谱》 CAS CSCD 北大核心 2013年第9期838-844,共7页
为了探索液相色谱-质谱联用(LC-MS)技术在快速识别中药及天然产物成分中的应用,以黄酮对照品为研究前体,药用植物小毛茛为研究对象,采用超高效液相色谱/二极管阵列检测器-电喷雾四极杆串联飞行时间质谱(UPLC/DAD-ESI/Q-TOF MS)分析了黄... 为了探索液相色谱-质谱联用(LC-MS)技术在快速识别中药及天然产物成分中的应用,以黄酮对照品为研究前体,药用植物小毛茛为研究对象,采用超高效液相色谱/二极管阵列检测器-电喷雾四极杆串联飞行时间质谱(UPLC/DAD-ESI/Q-TOF MS)分析了黄酮类化合物同系物及同分异构体的色谱、质谱特性。结果显示:黄酮氧苷和黄酮碳苷的紫外吸收光谱及二级质谱具有显著性差异,糖苷化位置同保留时间、二级质谱碎片及相对丰度具有相关性。将该方法应用于小毛茛茎叶醇提液的分析,结合其酸水解液的分析,解析了22个黄酮醇糖苷和3个苷元。方法简便,具有可操作性。 展开更多
关键词 超高效液相色谱-四极杆串联飞行时间质谱 黄酮类化合物 小毛茛
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HPLC-MS/MS法测定烟叶中7种植物内源激素 被引量:8
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作者 陈千思 卢紫舒 +14 位作者 沈少君 曾文龙 张月明 罗朝鹏 郑庆霞 王晨 张慧 翟妞 徐国云 金立峰 陈霞 于永杰 曹培健 刘萍萍 周会娜 《烟草科技》 EI CAS CSCD 北大核心 2018年第11期51-57,共7页
为获得烟草激素的质量分数数据,并分析其在抗性、生长发育等过程中的作用,采用高效液相色谱-三重四极杆质谱(HPLC-MS/MS)仪建立了同时分析烟叶中吲哚乙酸(IAA)、吲哚丁酸(IBA)、水杨酸(SA)、赤霉素(GA)、茉莉酸(JA)、脱落酸(ABA)和油菜... 为获得烟草激素的质量分数数据,并分析其在抗性、生长发育等过程中的作用,采用高效液相色谱-三重四极杆质谱(HPLC-MS/MS)仪建立了同时分析烟叶中吲哚乙酸(IAA)、吲哚丁酸(IBA)、水杨酸(SA)、赤霉素(GA)、茉莉酸(JA)、脱落酸(ABA)和油菜素内酯(BR)等7种植物激素质量分数的方法。采用多离子反应检测模式,获得7种植物激素的特征碎片离子峰,并测定了烟草花叶病毒(TMV)感染的烟叶样品。结果表明:(1)7种植物激素在线性范围内的相关系数(r)为0.998 6~0.999 5,方法的检出限为0.07~3.00μg/L,日内日间相对标准偏差(RSD)为0.44%~9.76%,回收率为77.82%~117.63%;(2)烟草花叶病毒感染极显著(p<0.01)地增加了烟叶中SA、IBA、JA、ABA的质量分数,显著(p<0.05)增加了烟叶中IAA的质量分数,同时显著(p<0.05)降低了烟叶中GA的质量分数;TMV病毒感染对BR的影响不大。该方法具有良好的灵敏度、精密度和回收率,可用于植物相关激素的同时分析。 展开更多
关键词 烟草 植物内源激素 高效液相色谱-串联质谱(HPLC-MS/MS) 烟草花叶病毒(TMV)
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液相色谱-串联质谱法测定双壳类水产品中原多甲藻酸类贝类毒素 被引量:6
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作者 孙兴权 郑秋月 +4 位作者 庞艳华 李一尘 肖珊珊 徐静 曹际娟 《分析化学》 SCIE EI CAS CSCD 北大核心 2013年第9期1423-1427,共5页
建立了双壳类水产品中原多甲藻酸贝类毒素Azaspiracid 1(AZA1)、Azaspiracid 2(AZA2)和Azaspir-acid 3(AZA3)的液相色谱-电喷雾串联质谱检测方法。选用具有基质代表性的扇贝、牡蛎和杂色蛤为研究对象,样品经80%甲醇-水混合溶液提取,正... 建立了双壳类水产品中原多甲藻酸贝类毒素Azaspiracid 1(AZA1)、Azaspiracid 2(AZA2)和Azaspir-acid 3(AZA3)的液相色谱-电喷雾串联质谱检测方法。选用具有基质代表性的扇贝、牡蛎和杂色蛤为研究对象,样品经80%甲醇-水混合溶液提取,正己烷脱脂、氯仿反提萃取并旋转蒸发浓缩后,MAX混合型阴离子交换反相吸附固相萃取柱净化,Luna C18色谱柱(150 mm×2.0 mm,5μm,Phenomenex公司)反相液相色谱法分离,乙腈和0.1%甲酸溶液组成的流动相梯度洗脱,正离子扫描,多反应监测(MRM)模式下电喷雾串联质谱法测定和确证,外标法定量。结果表明,3种原多甲藻酸类贝类毒素均在4 min内出峰,检出限均为1.5μg/kg,在0.3~30μg/L范围内线性良好,平均回收率大于80%,相对标准偏差(RSD)小于12%(n=6)。 展开更多
关键词 贝类毒素 原多甲藻酸 双壳类水产品 液相色谱-串联质谱
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液相色谱-串联四极杆飞行时间质谱和超高效液相色谱-串联三重四极杆质谱用于检测牛肉中的刚果红 被引量:9
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作者 林慧 徐春祥 +2 位作者 颜春荣 张征 王岁楼 《色谱》 CAS CSCD 北大核心 2013年第9期914-919,共6页
建立了牛肉中刚果红的检测方法。定性方法采用液相色谱-串联四极杆飞行时间质谱对未知物进行质谱谱图库匹配,定量分析采用超高效液相色谱-串联三重四极杆质谱。牛肉样品中的刚果红经液液萃取净化后,采用Agilent ZORBAX Eclipse Plus C18... 建立了牛肉中刚果红的检测方法。定性方法采用液相色谱-串联四极杆飞行时间质谱对未知物进行质谱谱图库匹配,定量分析采用超高效液相色谱-串联三重四极杆质谱。牛肉样品中的刚果红经液液萃取净化后,采用Agilent ZORBAX Eclipse Plus C18Rapid Resolution HD色谱柱(50mm×2.1mm,1.8μm)进行分离,流动相为95%(体积分数)甲醇,流速为0.2mL/min。AB 4000+三重四极杆质谱仪在电喷雾负离子化(ESI)及MRM模式下定量。结果显示,刚果红在0.03~1mg/L浓度范围内,线性关系良好(相关系数为0.999 8),精密度良好(RSD小于5%),回收率为88%~91%,检出限约为0.01mg/L。本方法快速简便,重现性好,可以为牛肉及其他肉制品中刚果红的定量提供良好的解决方案。 展开更多
关键词 高效液相色谱-串联四极杆飞行时间质谱 超高效液相色谱-串联三重四极杆质谱 刚果红 牛肉
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固相萃取-液相色谱串联质谱法同时测定养殖海水中17种喹诺酮类药物 被引量:9
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作者 董晓 李兆新 +3 位作者 孙晓杰 邢丽红 彭吉星 宋才湖 《渔业科学进展》 CSCD 北大核心 2017年第6期127-138,共12页
建立了固相萃取-液相色谱串联质谱法(SPE-LC-MS/MS)同时检测养殖海水中17种喹诺酮类药物残留的分析方法。海水经酸化处理后,采用HLB固相萃取柱富集、净化目标化合物,通过对比水样在不同上样p H、淋洗液与洗脱液等条件下的回收率,以此对... 建立了固相萃取-液相色谱串联质谱法(SPE-LC-MS/MS)同时检测养殖海水中17种喹诺酮类药物残留的分析方法。海水经酸化处理后,采用HLB固相萃取柱富集、净化目标化合物,通过对比水样在不同上样p H、淋洗液与洗脱液等条件下的回收率,以此对前处理方法进行优化。收集到的洗脱液经氮气吹干后用流动相定容至1 ml,待测。色谱流动相A相为0.1%甲酸水溶液,B相为乙腈,所有药物经梯度洗脱进行分离,在LC-MS/MS正离子模式的多反应监测模式下进行定性定量分析。17种化合物可以在10 min中内得到较好的分离,线性范围为1–200 ng/ml,线性相关系数均大于0.999,检出限均在2–10 ng/L范围内,定量限均在5–20 ng/L范围内。以空白海水为基质,在20、100、200 ng/L三个不同添加水平下采用内标法定量的加标回收率均在71.3%–125.0%范围内,相对标准偏差(RSD)为2.44%–12.27%(n=5)。采用该方法对黄海灵山湾近岸4个养殖场进行海水采集并检测,共检测出4种喹诺酮类药物,分别是恩诺沙星、氧氟沙星、诺氟沙星和环丙沙星,其中,恩诺沙星浓度最高。研究表明,该方法快速、可靠,适用于养殖海水中喹诺酮类药物的检测。 展开更多
关键词 喹诺酮类药物 固相萃取 液相色谱-串联质谱 养殖海水
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土壤中苯肽胺酸的测定及淋溶特性研究 被引量:5
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作者 赵欢欢 徐军 +5 位作者 吴艳兵 董丰收 刘新刚 吴小虎 鞠超 郑永权 《农药学学报》 CAS CSCD 北大核心 2015年第3期366-370,共5页
建立了反相条件下超高效液相色谱-串联质谱(UPLC-MS/MS)检测土壤中苯肽胺酸残留量的分析方法,并结合土壤薄层层析试验研究了苯肽胺酸在3种典型土壤(黑土、水稻土和红土)中的淋溶特性。结果表明:添加水平为0.1、1和10 mg/kg时,苯肽... 建立了反相条件下超高效液相色谱-串联质谱(UPLC-MS/MS)检测土壤中苯肽胺酸残留量的分析方法,并结合土壤薄层层析试验研究了苯肽胺酸在3种典型土壤(黑土、水稻土和红土)中的淋溶特性。结果表明:添加水平为0.1、1和10 mg/kg时,苯肽胺酸在土壤中的添加回收率为77.3%~99.4%,相对标准偏差(RSD)为1.3%~11.4%;其在3种土壤中的检出限(LOD)均低于1.0μg/kg。苯肽胺酸在吉林黑土中的比移值(Rf)为0.83,其移动性为可移动;在江苏水稻土中的Rf值为0.61,在湖南红土中的Rf值为0.62,移动性均为中等。 展开更多
关键词 苯肽胺酸 土壤 超高效液相色谱-串联质谱 淋溶 残留
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液相色谱-离子阱-飞行时间质谱法定性分析未知着色剂 被引量:15
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作者 胡莉 雷绍荣 +1 位作者 郭灵安 冀峰 《分析化学》 SCIE EI CAS CSCD 北大核心 2013年第1期110-114,共5页
使用理化性质鉴别、紫外可见分光光度计、超快速液相色谱仪-二极管阵列检测器-离子阱-飞行时间串联质谱仪(UFLC-PDA-MS-IT-TOF)共同对用于"染色橙"的未知着色剂进行定性分析,并用超高效液相色谱-串联质谱(UPLC-MS/MS)进行确... 使用理化性质鉴别、紫外可见分光光度计、超快速液相色谱仪-二极管阵列检测器-离子阱-飞行时间串联质谱仪(UFLC-PDA-MS-IT-TOF)共同对用于"染色橙"的未知着色剂进行定性分析,并用超高效液相色谱-串联质谱(UPLC-MS/MS)进行确证。通过紫外光谱确定未知着色剂特征吸收波长,再用液相色谱分离,用PDA检测器可知其出峰时间,用质谱检测可知与其它干扰物质是否分离。在确定干扰物质与未知着色剂进行分离后,可以得到未知着色剂的分子离子峰和五级质谱碎片,根据碎片离子,推导出了裂解途径,并使用分子式软件预测了未知着色剂分子式。综合实验结果,最后定性了用于"染色橙"的未知着色剂为"橘红2号",其分子式为C18H16N2O3(m/z 309.1932)。用超高效液相色谱-串联质谱将未知着色剂与橘红2号标准品的保留时间、离子对m/z 309.3>153.3和m/z 309.3>278.3及离子比率进行比对,确证定性结果是准确的。本实验为未知物定性提供了一个有效的思路和方法。 展开更多
关键词 橘红2号 未知着色剂 液相色谱-二极管阵列检测器-离子阱-飞行时间串联质谱 超高效液相色谱-串联质谱
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QuEChERS结合柱净化的超高效液相色谱-串联质谱法测定有机肥中46种抗生素 被引量:9
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作者 汪建妹 刘艳平 +4 位作者 陈静 吴慧珍 夏魏 李祖光 钱鸣蓉 《分析测试学报》 CAS CSCD 北大核心 2017年第2期218-223,共6页
建立了超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定有机肥中磺胺类、喹诺酮类、大环内酯类46种抗生素的分析方法。样品用乙腈-EDTA缓冲溶液(p H 10.0)提取,盐析后离心分层,乙腈层按Qu ECh ERS法,采用吸附剂净化;缓冲溶液层经HLB柱净化... 建立了超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定有机肥中磺胺类、喹诺酮类、大环内酯类46种抗生素的分析方法。样品用乙腈-EDTA缓冲溶液(p H 10.0)提取,盐析后离心分层,乙腈层按Qu ECh ERS法,采用吸附剂净化;缓冲溶液层经HLB柱净化。ACQUITY UPLC BEH C18柱用作色谱分离,以2mmol/L甲酸铵水溶液(含0.1%甲酸)-甲醇为流动相进行梯度洗脱;电喷雾电离源正离子(ESI+)多反应监测(MRM)模式检测,基质外标法定量。46种抗生素在1~200μg/L范围内线性关系良好,相关系数(r2)为0.996 6~0.999 9。在25,100,400μg/kg加标浓度下,3类抗生素的回收率分别为67.8%~95.6%,65.6%~89.4%和66.6%~107.8%,相对标准偏差为0.4%~11.9%;方法检出限(S/N=3)为0.6~4.6μg/kg,定量下限(S/N=10)为2.1~15.4μg/kg。 展开更多
关键词 超高效液相色谱-串联质谱法(uplc-ms/ms) 抗生素残留 有机肥 QuEChERS结合柱净化
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