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Optimization of Extraction Process for Total Flavonoids from Penthorum chinense Pursh and Comparison of Their Contents from Different Parts
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作者 Hongsheng LIU Jiqiang YAO +2 位作者 Xue BAI Jianying KANG Yefei YUAN 《Medicinal Plant》 2024年第2期10-14,共5页
[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experimen... [Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh. 展开更多
关键词 Penthorum chinense Pursh Total flavonoids Orthogonal experiments extraction process content determination
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Optimization of Extraction Process and Content Determination of Total Flavonoids from Sanguisorbae Radix and Carbonized Sanguisorba Root by Orthogonal Experimental Design 被引量:1
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作者 Fenglin WANG Yunzhang XU +1 位作者 Jingxia WANG Yuan LIU 《Medicinal Plant》 2017年第6期5-9,共5页
[Objectives] To optimize the extraction process of total flavonoids from Sanguisorbae Radix and carbonized Sanguisorba root,compare quality of different batches of Sanguisorbae Radix,study the effects of processing on... [Objectives] To optimize the extraction process of total flavonoids from Sanguisorbae Radix and carbonized Sanguisorba root,compare quality of different batches of Sanguisorbae Radix,study the effects of processing on the content of flavonoids,and provide scientific basis for reasonable utilization of Sanguisorbae Radix. [Methods] Test samples were prepared by heating,refluxing,and extraction,the extraction process was optimized by orthogonal experiment design,color was developed by NaNO_2-Al( NO_3)3-NaOH,and total flavonoids were measured by UV method at the wavelength of 510 nm. [Results] The linear relationship of rutin was excellent in the concentration range of 0. 1248 mg/mL-0. 5712 mg/mL,R^2= 0. 9997; the average recovery was 99. 67% and the RSD was 0. 70%. The optimum extraction conditions were as follows: the volume fraction of ethanol was 50%,the extraction temperature was 90℃,the extraction time was 90 min,and the solid-to-liquid ratio was 1∶ 20( g/mL). [Conclusions] After optimization of the extraction process,the extraction rate of total flavonoids in samples of Sanguisorbae Radix was significantly increased; there was certain difference in the content of total flavonoids between different batches of Sanguisorbae Radix and processed products; the total flavonoids significantly declines in carbonized sanguisorba root,and the influence of processing on its curative effect was to be further studied. 展开更多
关键词 Sanguisorbae RADIX Carbonized sanguisorba ROOT TOTAL FLAVONOIDS extraction process content determination
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TLC Identification and Extraction Process of Rubiasin-1-methyl Ether from Yao Medicine Chuanlianzhu
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作者 Jingrong LU Jiangcun WEI +3 位作者 Xiumei MA Bing QING Meiyan QIU Wen ZHONG 《Medicinal Plant》 2024年第3期35-38,共4页
[Objectives]To establish a thin-layer chromatography(TLC)method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu(Damnacanthus giganteus).[Methods]A silica gel G thin-layer plate was adopte... [Objectives]To establish a thin-layer chromatography(TLC)method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu(Damnacanthus giganteus).[Methods]A silica gel G thin-layer plate was adopted for TLC.Petroleum ether(60-90℃)-chloroform-methanol-water(7:15:3:1)was used as the developing solvent and inspected under ultraviolet lamp(365 nm).The content was determined by Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm),mobile phase:acetonitrile-0.2%phosphoric acid gradient elution,detection wavelength 277 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The spots of 10 Chuanlianzhu samples from different origins showed the same color at the same position as the control,and the spots were clear and specific.The injection volume of rubiadin-1-methyl ether showed a good linear relationship in the range of 2.90-145μg(R=0.9996).The average recovery rate of rubiadin-1-methyl ether in the low,medium and high dose groups of Yao Medicine Chuanlianzhu was 98.72%,and RSD=1.78%.[Conclusions]This method can effectively identify Yao Medicine Chuanlianzhu medicinal materials and accurately determine the content of rubiadin-1-methyl ether in the medicinal materials.It provides a scientific basis for the development and utilization of Yao Medicine Chuanlianzhu medicinal resources. 展开更多
关键词 Chuanlianzhu THIN-LAYER chromatography (TLC) extraction process Rubiadin-1-methyl ETHER content determination
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Extraction Process and Content Determination of Total Flavonoids in Ginkgo( Ginkgo biloba L. ) Leaves 被引量:2
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作者 Dengfeng ZOU Hua ZHU +1 位作者 Binghua QIN Xiaohua WANG 《Agricultural Biotechnology》 CAS 2019年第1期171-175,共5页
[Objectives]This study was conducted to study the extraction process and the content determination of flavonoids in ginkgo( Ginkgo biloba L.) leaves.[Methods]Ethanol extraction and methanol extraction of total flavono... [Objectives]This study was conducted to study the extraction process and the content determination of flavonoids in ginkgo( Ginkgo biloba L.) leaves.[Methods]Ethanol extraction and methanol extraction of total flavonoids in ginkgo leaves were studied,and the optimal extraction conditions for flavonoids were determined by orthogonal test; and with quercetin as reference substance,total flavonoid content in ginkgo leaves was determined by UV spectrophotometry.[Results]The optimal extraction process was 4 h of Soxhlet extraction with methanol; and the total flavonoid contents had a good linear relation in the range of 0. 006 5-0. 039 mg/ml( R^2= 0. 999 9),the average content was stabilized at 1. 135%,and the average recovery of the method was 102. 0%. [Conclusions]This study selected the optimal extraction process for total flavonoids in ginkgo leaves. The test method is simple with high accuracy and precision,and is suitable for the extraction and determination of total flavonoids in ginkgo leaves. 展开更多
关键词 GINKGO biloba L. TOTAL FLAVONOIDS extraction process content determination
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Extraction Process and Content Determination of Caffeic Acid in Laggera alata from Different Production Areas of Guangxi 被引量:1
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作者 Jiangcun WEI Yong CHEN +5 位作者 Zujie QIN Jiabao MA Shengbin CHEN Zuliang QUE Jinzhou LI Zijun CHEN 《Medicinal Plant》 CAS 2018年第4期73-76,共4页
[Objectives] To establish a method for determining the content of Laggera alata( D. Don) Sch. Bip. Ex Oliv. using caffeic acid the target component,and to compare the content of caffeic acid in the medicinal materials... [Objectives] To establish a method for determining the content of Laggera alata( D. Don) Sch. Bip. Ex Oliv. using caffeic acid the target component,and to compare the content of caffeic acid in the medicinal materials of L. alata in different production areas of Guangxi.[Methods]The content was determined by Inertsil~ODS-3 chromatographic column C_(18)( 4. 60 mm × 250 mm,5 μm,mobile phase: acetonitrile-0. 1% phosphoric acid( 22∶ 78),detection wavelength: 320 nm,flow rate: 1. 0 m L/min,column temperature: 30℃,and injection volume: 10 μL. [Results] The caffeic acid showed a good linear relationship in the range of injection volume of 0. 025 92-0. 259 2 μg( R =0. 999 5). The average recovery rate was 98. 33%( RSD = 1. 85%). L. alata in different production areas of Guangxi contained the caffeic acid,and there was a great difference in the caffeic acid. L. alata in Baise had the highest content of caffeic acid,while that in Guilin had the lowest content of caffeic acid. [Conclusions]This method can accurately determine the content of caffeic acid and is expected provide a scientific basis for the development and utilization of herbal medicine L. alata. 展开更多
关键词 Laggera alata(D.Don) Sch.Bip.Ex Oliv. HPLC Caffeic acid extraction process content determination
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Extraction Process and Content Determination of Caffeic Acid in Zhuang Medicine Cryptolepis buchananii
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作者 Chenyan LIANG Lu ZHANG +2 位作者 Yunli TANG Liuyan HE Ganneng HUANG 《Medicinal Plant》 CAS 2019年第6期15-19,共5页
[Objectives]To establish a method for determining the content of caffeic acid in Zhuang Medicine Cryptolepis buchananii.[Methods]The content of caffeic acid in C.buchananii leaves was determined by high performance li... [Objectives]To establish a method for determining the content of caffeic acid in Zhuang Medicine Cryptolepis buchananii.[Methods]The content of caffeic acid in C.buchananii leaves was determined by high performance liquid chromatography(HPLC).[Results]The results of HPLC determination showed that the RSD values were less than 3%,and the content of caffeic acid in 10 batches of C.buchananii leaves was in the range of 0.0965%-0.4772%.[Conclusions]This method is accurate and reliable,has good linear relationship and high precision,and can be used for quality evaluation of C.buchananii. 展开更多
关键词 Cryptolepis buchananii Caffeic ACID High performance LIQUID chromatography(HPLC) extraction process content determination
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Thin-layer Identification,Extraction Process and Content Determination of Chlorogenic Acid in Laggera alata(D.Don)Sch.Bip.ex Oliv.
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作者 Qiaofeng QIN Xue JIANG +5 位作者 Jiangcun WEI Jiabao MA Jingrong LU Lifu WEI Xiumei MA Xuanyang ZENG 《Medicinal Plant》 CAS 2020年第6期72-76,共5页
[Objectives]This paper aims to establish thin-layer identification and content determination method for Laggera alata(D.Don)Sch.Bip.ex Oliv.with chlorogenic acid as the index component and compare the content of chlor... [Objectives]This paper aims to establish thin-layer identification and content determination method for Laggera alata(D.Don)Sch.Bip.ex Oliv.with chlorogenic acid as the index component and compare the content of chlorogenic acid in L.alata from different places in Guangxi.[Methods]Silica gel GF254 thin-layer plate was used for identification under an ultraviolet lamp(365 nm),with butyl acetate-formic acid-water(V∶V∶V=7∶2.5∶2.5)as a developing agent.The content of chlorogenic acid was determined under the following chromatographic conditions:column,Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase,methanol-0.1%phosphoric acid(28∶72);detection wavelength,329 nm;flow rate,1.0 mL/min;column temperature,25℃;and injection volume,10μL.[Results]Chlorogenic acid can be detected by thin layer chromatography with clear spot and good specificity.Chlorogenic acid showed a good linear relationship in the injection amount range of 0.099-0.99μg(R^(2)=0.9999).The content of chlorogenic acid in L.alata varied greatly among the 10 different producing areas in Guangxi.L.alata produced in Dee Township,Longlin,Baise,Guangxi showed the highest chlorogenic acid content,and that produced in Shangsi County and Pingle County showed the lowest chlorogenic acid content.[Conclusions]This method can effectively identify L.alata and accurately determine the content of chlorogenic acid,thereby providing a scientific basis for the development and utilization of L.alata resources. 展开更多
关键词 Laggera alata Chlorogenic acid content determination Thin-layer identification extraction process
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Extraction Process and Quality Standard of Danshen Jianxin Capsules 被引量:1
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作者 Chengtong LIU Xinying MO +5 位作者 Silu HE Donglian CHEN Guilin YANG Hongsheng TAN Peng LIANG Haisheng ZENG 《Medicinal Plant》 CAS 2023年第1期25-28,共4页
[Objectives]This study was conducted to establish the quality standard of Danshen Jianxin Capsules.[Methods]Orthogonal design was adopted to optimize the water extraction process by taking the amount of water added,ex... [Objectives]This study was conducted to establish the quality standard of Danshen Jianxin Capsules.[Methods]Orthogonal design was adopted to optimize the water extraction process by taking the amount of water added,extraction time and extraction times as factors,and the content of tanshinone I as the index.Salviae Miltiorrhizae,Notoginseng Radix Et Rhizoma and Radix Astragali in the capsules were qualitatively identified by TLC,and the content of tanshinone I in the capsules was determined by HPLC.[Results]The best water extraction process included the steps of adding 12 times of water each time,extracting the materials twice,for 1 h each time.In TLC identification,the test samples showed spots of the same color at the positions corresponding to tanshinone II A,ginsenoside Rg1,notoginsenoside R1 and astragaloside IV reference samples.[Conclusions]Danshen Jianxin Capsules was prepared by the water extraction method,which is characterized by high efficiency and being suitable for mass production.The quality control method is reliable,fast and accurate,which can effectively control the quality of the product. 展开更多
关键词 Danshen Jianxin Capsules extraction process content determination TLC identification Quality standard
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Extraction Process of Zhuang Medicine Fumigation Lotion
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作者 Jiangcun WEI Meiyan QIU +4 位作者 Bing QING Xianyi SHI Yinghong HUANG Xian PENG Wen ZHONG 《Medicinal Plant》 CAS 2023年第5期29-31,41,共4页
[Objectives]To establish the extraction process and quality standard method of Zhuang medicine fumigation lotion.[Methods]The orthogonal design method was employed to optimize the water extraction process with the amo... [Objectives]To establish the extraction process and quality standard method of Zhuang medicine fumigation lotion.[Methods]The orthogonal design method was employed to optimize the water extraction process with the amount of water added,decocting time and extraction times as factors,and syringin content and dry extract yield as indexes.The content of syringin was determined by high performance liquid chromatography.[Results]The best water extraction process was:soaking in water for 1 h,decocting twice,added 10 times the amount of water each time,decocting for 1 h.The average content of syringin in 3 batches was 0.98 mg/g,and the average dry extract yield was 26.07%.[Conclusions]The project adopts water extraction method to prepare Zhuang medicine fumigation lotion,which has the characteristics of high efficiency and suitable for large-scale production.The quality control method is reliable,rapid and accurate,and can effectively control the quality of the lotion. 展开更多
关键词 Zhuang medicine fumigation lotion extraction process content determination
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TLC Identification of Laggerae Alatae Herba and Extraction Process of Chlorogenic Acid
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作者 Huimin ZHOU Xiumei MA +5 位作者 Ting PENG Chunmei LIU Xue JIANG Jiangcun WEI Wen ZHONG Chunli TANG 《Medicinal Plant》 CAS 2022年第3期32-35,共4页
[Objectives]Taking chlorogenic acid as index component,TLC and content determination method of Laggerae Alatae Herba were established.[Methods]TLC identification used silica gel G thin-layer plate,and butyl acetate∶f... [Objectives]Taking chlorogenic acid as index component,TLC and content determination method of Laggerae Alatae Herba were established.[Methods]TLC identification used silica gel G thin-layer plate,and butyl acetate∶formic acid∶water(7∶2.5∶2.5)was taken as developing agent,and it was inspected under ultraviolet lamp(365 nm).The content was determined by chromatographic column Inertsil ODS-3 C_(18)(4.60 mm×250 mm,5μm).Mobile phase:methanol-0.1%phosphoric acid(28∶72);detection wavelength:329 nm;flow speed:1.0 mL/min;column temperature:25℃;injection volume:10μL.[Results]Chlorogenic acid can be detected by TLC,with clear spots and good specificity.When injection volume of chlorogenic acid was between 0.099 and 0.990μg(R^(2)=0.9999),there was good linear relationship.In low,medium and high sample adding groups of Laggerae Alatae Herba,average recovery rates of chlorogenic acids were 98.80%(RSD=2.09%),98.24%(RSD=1.96%)and 99.65%(RSD=2.15%).[Conclusions]The method could effectively identify medicinal material Laggerae Alatae Herba,and accurately measure the content of chlorogenic acid in Laggerae Alatae Herba,thereby providing a scientific basis for developing and using medicinal resources of Laggerae Alatae Herba. 展开更多
关键词 Laggera alata Chlorogenic acid content determination TLC identification extraction process
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金花葵花黄酮超声辅助提取工艺优化及性能
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作者 王欣 刘雨默 +3 位作者 郝静 赵海宁 李萌 王昌涛 《精细化工》 EI CAS CSCD 北大核心 2024年第6期1310-1317,共8页
以金花葵花为原料,采用超声辅助提取法提取了金花葵花黄酮,通过单因素实验和正交实验优化了提取工艺,评估了最佳工艺条件下提取的金花葵花黄酮的抗氧化活性及安全性。结果表明,金花葵花黄酮最佳提取工艺为:料液比(g∶m L)1∶15、体积分... 以金花葵花为原料,采用超声辅助提取法提取了金花葵花黄酮,通过单因素实验和正交实验优化了提取工艺,评估了最佳工艺条件下提取的金花葵花黄酮的抗氧化活性及安全性。结果表明,金花葵花黄酮最佳提取工艺为:料液比(g∶m L)1∶15、体积分数80%的乙醇水溶液为溶剂、超声功率300 W、超声时间35 min、提取温度75℃,在该条件下,金花葵花黄酮提取率为89.97%。质量浓度为1.00 g/L金花葵花黄酮对1,1-二苯基-2-苦基苯肼自由基、羟基自由基和2,2’-联氮-双(3-乙基-苯并噻唑-6-磺酸)自由基的清除率最高,分别为94.81%、95.24%、82.80%;对三者的半数抑制质量浓度(IC_(50))分别为106.00、70.06和213.80μg/m L。金花葵花黄酮质量浓度≤0.4 g/L时,其对红细胞溶血率均<10%,属于轻度刺激;质量浓度为1.00 g/L的金花葵花黄酮对鸡胚绒毛尿囊膜无溶血。 展开更多
关键词 金花葵花 黄酮 超声辅助提取法 工艺优化 抗氧化活性 安全性 医药原料
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响应面优化超声辅助乙醇提取银杏叶莽草酸工艺
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作者 郁万文 杨森 +1 位作者 赵丹 吴项乾 《北方园艺》 CAS 北大核心 2024年第19期99-107,共9页
以银杏干叶为试材,采用超声辅助70%乙醇提取莽草酸,通过单因素试验和响应面试验优化提取工艺,研究了料液比、提取温度、提取时间、提取次数对莽草酸提取得率的影响,优化了银杏叶莽草酸的最佳提取工艺,以期为银杏叶莽草酸的提取提供参考... 以银杏干叶为试材,采用超声辅助70%乙醇提取莽草酸,通过单因素试验和响应面试验优化提取工艺,研究了料液比、提取温度、提取时间、提取次数对莽草酸提取得率的影响,优化了银杏叶莽草酸的最佳提取工艺,以期为银杏叶莽草酸的提取提供参考依据。结果表明:最佳工艺条件为料液比1∶8 g·mL^(-1),提取温度66℃,提取时间65 min(每提取1次),提取次数2次。该条件下测得银杏叶莽草酸提取得率量为1.805%,与理论得率(1.883%)接近。 展开更多
关键词 银杏叶 莽草酸 超声辅助提取 工艺优化
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超声波微波协同浸提工艺优化及速溶红茶产品品质分析
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作者 付静 沈小萌 +3 位作者 杨晨曦 刘宁宁 赵锦 李新生 《食品研究与开发》 CAS 2024年第16期113-121,共9页
为提高速溶红茶品质,研究超声波微波协同浸提工艺,考察不同因素(超声时间、超声功率、微波时间、微波功率、微波温度)对茶黄素总量及其单体组分含量的影响,并通过响应面优化条件。在此基础上对速溶红茶产品进行感官审评和主要理化指标... 为提高速溶红茶品质,研究超声波微波协同浸提工艺,考察不同因素(超声时间、超声功率、微波时间、微波功率、微波温度)对茶黄素总量及其单体组分含量的影响,并通过响应面优化条件。在此基础上对速溶红茶产品进行感官审评和主要理化指标的测定。结果表明:最优工艺条件为超声时间31 min、超声功率360 W、微波时间9 min、微波温度52℃、微波功率203 W。在该工艺条件下,速溶红茶的汤色红亮,香气醇厚,感官审评总分为91.73,其中香气90.00、汤色91.80、组织形态92.40、滋味94.20;茶黄素总含量高达3.74%,茶多酚含量为24.11%。 展开更多
关键词 速溶红茶 超声波微波协同浸提 茶黄素 工艺优化 品质分析
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蚕丝含油率微波萃取试验方法探讨
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作者 吴盼 薛文良 +1 位作者 钱竞芳 周小进 《国际纺织导报》 2024年第5期21-25,共5页
针对目前蚕丝含油率检测效率低、耗时长、资源浪费大的问题,选用微波萃取工艺,设计4因素3水平的正交试验,分析比较微波萃取法与索式萃取法在蚕丝含油率检测方面的应用,探讨微波萃取法的测试条件。试验结果表明:微波萃取法相比索式萃取... 针对目前蚕丝含油率检测效率低、耗时长、资源浪费大的问题,选用微波萃取工艺,设计4因素3水平的正交试验,分析比较微波萃取法与索式萃取法在蚕丝含油率检测方面的应用,探讨微波萃取法的测试条件。试验结果表明:微波萃取法相比索式萃取法检测准确度更高,过程更环保,且检测时间和试剂用量更少;微波萃取温度、萃取时间、萃取功率及乙酸乙酯用量各因素对样品含油率测试结果的影响从大到小依次为萃取温度、乙酸乙酯用量、萃取时间和萃取功率。最优检测条件为:萃取温度67℃,乙酸乙酯用量40 mL,萃取时间15 min,萃取功率800 W。 展开更多
关键词 蚕丝 含油率 微波萃取 索式萃取 工艺优化
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舒心解郁颗粒制备工艺及关键成分含量测定研究 被引量:1
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作者 谢先娇 彭进城 +7 位作者 左池靖 张静 梁万徽 汪杰 訾苗苗 韩双 方玲 彭灿 《海南医学院学报》 CAS 北大核心 2024年第6期413-420,共8页
目的:对舒心解郁颗粒的制备工艺进行研究,采用高效液相色谱法同时测定舒心解郁颗粒中7种成分的方法,以期为舒心解郁颗粒质量评价与临床应用提供科学依据。方法:采用正交试验优选处方中药材的煎煮工艺,以浸出率为指标,优选提取工艺。对... 目的:对舒心解郁颗粒的制备工艺进行研究,采用高效液相色谱法同时测定舒心解郁颗粒中7种成分的方法,以期为舒心解郁颗粒质量评价与临床应用提供科学依据。方法:采用正交试验优选处方中药材的煎煮工艺,以浸出率为指标,优选提取工艺。对辅料、药辅比、润湿剂及干燥温度进行考察,优选成型工艺。同时采用HPLC法测定制剂中洋川芎内酯Ⅰ、阿魏酸、斯皮诺素、芍药苷、3,5-O-二咖啡酰基奎宁酸、绿原酸、没食子酸的含量。结果:确定最优提取工艺:加入12倍量水,每次40 min,提取3次。成型工艺:用糊精作辅料,药辅比1∶1.8,80%乙醇作为润湿剂,65℃干燥。采用优选的制备工艺生产6批舒心解郁颗粒,建立成品中洋川芎内酯Ⅰ、阿魏酸、斯皮诺素、芍药苷、3,5-O-二咖啡酰基奎宁酸、绿原酸、没食子酸含量测定方法,测定舒心解郁颗粒成分含量。结论:该研究建立的舒心解郁颗粒制备工艺稳定合理、成型率高,为大批量生产舒心解郁颗粒提供了切实可行的方法,且本研究建立的含量测定方法,具有操作简便,重复性和稳定性好等特点,可为舒心解郁颗粒及相似的中药复方制剂的质量控制研究提供借鉴。 展开更多
关键词 舒心解郁颗粒 工艺优化 指标成分 含量测定 正交实验
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Box-Behnken响应面法优化“五味子-吴茱萸”药对的提取工艺及含量测定 被引量:1
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作者 庞成国 韩华 董培良 《中医药导报》 2024年第6期39-43,78,共6页
目的:优化“五味子-吴茱萸”药对提取工艺并测定其不同配伍的化学成分含量。方法:利用Box-Behnken设计在单因素考察的基础上考察“五味子-吴茱萸”药对的提取工艺,确定最佳提取工艺,并通过高效液相色谱法测定“五味子-吴茱萸”的化学含... 目的:优化“五味子-吴茱萸”药对提取工艺并测定其不同配伍的化学成分含量。方法:利用Box-Behnken设计在单因素考察的基础上考察“五味子-吴茱萸”药对的提取工艺,确定最佳提取工艺,并通过高效液相色谱法测定“五味子-吴茱萸”的化学含量,确定药对的最佳比例。结果:“五味子-吴茱萸”药对最优提取工艺为乙醇体积分数70%,料液比1∶15(g/mL),提取时间60 min,提取次数3次。该提取工艺科学可靠。结论:本研究方法简单可行,可为“五味子-吴茱萸”药对的开发利用及相关研究提供参考。 展开更多
关键词 五味子 吴茱萸 药对 提取工艺 单因素试验 Box-Behnken响应面试验 含量测定
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正交试验优选壮药龙路通颗粒提取工艺研究 被引量:1
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作者 王博 杨梅 +2 位作者 岳桂华 黄秋洁 孙宗喜 《广东化工》 CAS 2024年第8期41-43,13,共4页
目的:优选壮药龙路通颗粒提取工艺。方法:用高效液相色谱法测定三七皂苷R_(1),人参皂苷Rg_(1)和Rb_(1),并以出膏率、三七皂苷R_(1),人参皂苷Rg_(1)和Rb_(1)含量为评价指标。采用单因素法考察加水量、提取时间和提取次数,并通过正交试验... 目的:优选壮药龙路通颗粒提取工艺。方法:用高效液相色谱法测定三七皂苷R_(1),人参皂苷Rg_(1)和Rb_(1),并以出膏率、三七皂苷R_(1),人参皂苷Rg_(1)和Rb_(1)含量为评价指标。采用单因素法考察加水量、提取时间和提取次数,并通过正交试验优选壮药龙路通颗粒最佳提取工艺。结果:以三七皂苷R_(1),人参皂苷Rg_(1)和Rb_(1)的含量作为龙路通颗粒质控标准,测得龙路通颗粒中3种指标性成分的质量分数分别为三七皂苷R_(1)45.6~60.6 mg/g,人参皂苷Rg_(1)159.3~234.4 mg/g,和人参皂苷Rb_(1)143.8~246.0 mg/g;龙路通颗粒最佳提取工艺为加水煎煮提取3次,第一次加12倍量水,第二、三次分别加10倍量水,每次1.5 h。结论:优选的提取工艺合理可行,为壮药龙路通颗粒的生产及进一步开发提供了实验依据。 展开更多
关键词 壮药龙路通颗粒 正交试验 提取工艺 出膏率 含量测定
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运脾消食方提取工艺放大影响因素研究
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作者 林欣荣 高紫薇 +2 位作者 束雅春 赵霞 吴磊 《中成药》 CAS CSCD 北大核心 2024年第2期391-396,共6页
目的考察运脾消食方提取工艺放大影响因素。方法HPLC法测定儿茶素、阿魏酸、花旗松素、异牡荆素、芸香柚皮苷、白术内酯Ⅱ、柚皮苷、桑色素、橙皮苷、木犀草素、常春藤皂苷元、白术内酯I、柚皮素、橙皮素的含量,建立指纹图谱,评价容器... 目的考察运脾消食方提取工艺放大影响因素。方法HPLC法测定儿茶素、阿魏酸、花旗松素、异牡荆素、芸香柚皮苷、白术内酯Ⅱ、柚皮苷、桑色素、橙皮苷、木犀草素、常春藤皂苷元、白术内酯I、柚皮素、橙皮素的含量,建立指纹图谱,评价容器体积、火候、投料量对各成分含量的影响。结果15批样品指纹图谱相似度均大于0.995。14种成分在各自范围内线性关系良好(r>0.9990),平均加样回收率96.4%~103.3%,RSD 0.5%~2.7%。火候的影响程度大于提取容器体积、投料量。结论多成分含量测定结合指纹图谱可为运脾消食方提取工艺放大后一致性评价的共性技术提供数据基础和理论参考。 展开更多
关键词 运脾消食方 提取工艺放大 影响因素 含量测定 指纹图谱 HPLC
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不同工艺对养肝降草合剂整体质量的影响
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作者 刘玉鑫 朱亚苹 +1 位作者 田立元 吴磊 《中成药》 CAS CSCD 北大核心 2024年第7期2157-2163,共7页
目的考察提取、浓缩、离心、灭菌工艺对养肝降草合剂整体质量的影响。方法分析采用菲罗门LunaC_(18)色谱柱(250mm×4.6mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1mL/min;柱温20℃。建立HPLC指纹图谱,测定红景天苷、栀子苷、... 目的考察提取、浓缩、离心、灭菌工艺对养肝降草合剂整体质量的影响。方法分析采用菲罗门LunaC_(18)色谱柱(250mm×4.6mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1mL/min;柱温20℃。建立HPLC指纹图谱,测定红景天苷、栀子苷、连翘酯苷A、特女贞苷、甘草酸、白术内酯Ⅲ的含量,并计算其转移率。结果15批不同工艺下样品指纹图谱中有20个共有峰,相似度大于0.950。6种成分在各自范围内线性关系良好(r≥0.9990),平均加样回收率96.96%~106.69%,RSD0.97%~2.87%,转移率大于80%,总转移率大于90%。结论养肝降草合剂在不同工艺下均具有较好的稳定性。 展开更多
关键词 养肝降草合剂 提取工艺 浓缩工艺 离心工艺 灭菌工艺 HPLC指纹图谱 含量测定
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西洋参花多糖的提取工艺优化及其含量测定
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作者 徐清华 王国明 +6 位作者 谢丽娟 宋莹莹 郭畅冰 冯惠柳 李健豪 于晶 徐芳菲 《数理医药学杂志》 CAS 2024年第11期806-814,共9页
目的探究西洋参花多糖最佳水提工艺,并建立西洋参花多糖含量的检测方法。方法利用水提法提取西洋参花多糖,通过单因素试验优化西洋参花多糖提取温度,正交试验以西洋参花多糖提取率为指标优化工艺参数。采用苯酚-硫酸法测定西洋参花多糖... 目的探究西洋参花多糖最佳水提工艺,并建立西洋参花多糖含量的检测方法。方法利用水提法提取西洋参花多糖,通过单因素试验优化西洋参花多糖提取温度,正交试验以西洋参花多糖提取率为指标优化工艺参数。采用苯酚-硫酸法测定西洋参花多糖含量,并进行方法学研究。结果最佳提取工艺为95℃提取2.5 h,提取3次,料液比为1∶20(倍数),西洋参花多糖提取率为7.01%,人参花多糖提取率为6.54%,西洋参花多糖含量高于人参花。方法学试验显示,D-无水葡萄糖在65~325μg范围内与吸光度呈现较好的线性关系,平均加样回收率可达99.07%。结论该工艺有较高的提取效率,检测方法稳定可行,可为进一步分析西洋参花多糖组分及生理学评价提供试验依据。 展开更多
关键词 西洋参花 多糖 正交试验 工艺优化 含量测定
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