Microbes thrive and,in turn,influence the earth's environment,but most are poorly understood because of our limited capacity to reveal their natural diversity and function.Developing novel tools and effective stra...Microbes thrive and,in turn,influence the earth's environment,but most are poorly understood because of our limited capacity to reveal their natural diversity and function.Developing novel tools and effective strategies are critical to ease this dilemma and will help to understand their roles in ecology and human health.Recently,droplet microfluidics is emerging as a promising technology for microbial studies with value in microbial cultivating,screening,and sequencing.This review aims to provide an overview of droplet micro flu idics techniques for microbial research.First,some critical points or steps in the microfluidic system are introduced,such as droplet stabilization,manipulation,and detection.We then highlight the recent progress of droplet-based methods for microbiological applications,from high-throughput single-cell cultivation,screening to the targeted or whole-genome sequencing of single cells.展开更多
Microbial communities in marine habitats are regarded as underexplored reservoirs for discovering new natural products with potential application.However,only a few microbes in nature can be cultivated in the laborato...Microbial communities in marine habitats are regarded as underexplored reservoirs for discovering new natural products with potential application.However,only a few microbes in nature can be cultivated in the laboratory.This has led to the development of a variety of isolation and cultivation methods,and in situ cultivation is one of the most popular.Diverse in situ cultivation methods,with the same basic principle,have been applied to a variety of environmental samples.Compared with conventional approaches,these new methods are able to cultivate previously uncultured and phylogenetically novel microbes,many with biotechnological potential.This review introduces the various in situ cultivation methods for the isolation of previously uncultured microbial species and their potential for marine microbial resource mining.Furthermore,studies that investigated the key and previously unidentified mechanisms of growing uncultivated microorganisms by in situ cultivation,which will shed light on the understanding of microbial uncultivability,were also reviewed.展开更多
基金This work was financially supported by National Key Research and Development Program of China(2018YFC0310703)China Ocean Mineral Resources R&D Association(DY135-B-02)+2 种基金National Natural Science Foundation of China(91951103,91951105,2182240&31970091)Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDB-SSW-SMC008)Center for Ocean Mega-Science,Chinese Academy of Sciences(KEXUE2019GZ05).
文摘Microbes thrive and,in turn,influence the earth's environment,but most are poorly understood because of our limited capacity to reveal their natural diversity and function.Developing novel tools and effective strategies are critical to ease this dilemma and will help to understand their roles in ecology and human health.Recently,droplet microfluidics is emerging as a promising technology for microbial studies with value in microbial cultivating,screening,and sequencing.This review aims to provide an overview of droplet micro flu idics techniques for microbial research.First,some critical points or steps in the microfluidic system are introduced,such as droplet stabilization,manipulation,and detection.We then highlight the recent progress of droplet-based methods for microbiological applications,from high-throughput single-cell cultivation,screening to the targeted or whole-genome sequencing of single cells.
基金This work was funded by the National Natural Science Foundation of China(41776168,31600016)the National 111 Project of China(D16013)Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Development Fund.
文摘Microbial communities in marine habitats are regarded as underexplored reservoirs for discovering new natural products with potential application.However,only a few microbes in nature can be cultivated in the laboratory.This has led to the development of a variety of isolation and cultivation methods,and in situ cultivation is one of the most popular.Diverse in situ cultivation methods,with the same basic principle,have been applied to a variety of environmental samples.Compared with conventional approaches,these new methods are able to cultivate previously uncultured and phylogenetically novel microbes,many with biotechnological potential.This review introduces the various in situ cultivation methods for the isolation of previously uncultured microbial species and their potential for marine microbial resource mining.Furthermore,studies that investigated the key and previously unidentified mechanisms of growing uncultivated microorganisms by in situ cultivation,which will shed light on the understanding of microbial uncultivability,were also reviewed.
