Objective： To assess the clinical efficacy of electroacupuncture for treatment of unstable bladder. Methods： Patients were randomly divided into electroacupuncture and control groups and the curative effects were ev...Objective： To assess the clinical efficacy of electroacupuncture for treatment of unstable bladder. Methods： Patients were randomly divided into electroacupuncture and control groups and the curative effects were evaluated according to improvement in clinical symptom and quality of life scores after treatment. Results： The observation indices were significantly lower after than before treatment in both groups （P〈0.05）. There was no significant difference in clinical symptom score between the two groups （P〉0.05）. Quality of life score was significantly decreased in the electroacupuncture group as compared with that in the manipulation group （P〈0.05）. Conclusion： Both treatments improve voiding difficulty. The degree of satisfaction to quality of life is larger in the electroacupuncture group than in the manipulation group. Clinical symptom score tends to be lower in the electroacupuncture group than in the manipulation group.展开更多
Background Unstable bladder is one of the common clinical dysfunctions of the lower urinary tract. Gap junctions （GJs） are the plaques of aqueous channels that facilitate electrical and metabolic communication betwe...Background Unstable bladder is one of the common clinical dysfunctions of the lower urinary tract. Gap junctions （GJs） are the plaques of aqueous channels that facilitate electrical and metabolic communication between the intracellular compartments of adjacent cells, exchange of nutrients and ions between connected cells and transfer of electrical signals In the present study we investigated the quantitative alterations of the GJ in the rat detrusor muscle and its functional changes related to the developing of unstable bladder （USB）. Methods Thirteen female Wistar rats （study group） with obstructive unstable bladder as determined by urodynamic study and 10 sham-operated rats （control group） were sacrificed at 6 weeks after surgery. Cystometric investigation, and the content and distribution of the GJ protein connexin 43 （Cx43） in the detrusors which were taken from the bladder of the rats were studied by Western blot and laser confocal microscopy with a double label immunohistochemistry technique. Results The expression of Cx43 was found adjacent to the detrusor with the laser confocal microscopy. The Cx43 expression increased markedly in the study group （pixel density 29.5±13.9, staining size （17.9±8.8） μm2） compared with the control group （pixel density 14.2±2.2, staining size （5.7±3.1） pm2, P 〈0.05）. Western blot analysis demonstrated that Cx43 in the study group （the average gray level was 31.066） was significantly higher than in the control group （the average gray level was 11.701, P 〈0.01 ）. Conclusion The increase of GJ leading to a intercellular excitatory communication is one of the important mechanisms related to developing unstable bladder.展开更多
文摘Objective： To assess the clinical efficacy of electroacupuncture for treatment of unstable bladder. Methods： Patients were randomly divided into electroacupuncture and control groups and the curative effects were evaluated according to improvement in clinical symptom and quality of life scores after treatment. Results： The observation indices were significantly lower after than before treatment in both groups （P〈0.05）. There was no significant difference in clinical symptom score between the two groups （P〉0.05）. Quality of life score was significantly decreased in the electroacupuncture group as compared with that in the manipulation group （P〈0.05）. Conclusion： Both treatments improve voiding difficulty. The degree of satisfaction to quality of life is larger in the electroacupuncture group than in the manipulation group. Clinical symptom score tends to be lower in the electroacupuncture group than in the manipulation group.
文摘Background Unstable bladder is one of the common clinical dysfunctions of the lower urinary tract. Gap junctions （GJs） are the plaques of aqueous channels that facilitate electrical and metabolic communication between the intracellular compartments of adjacent cells, exchange of nutrients and ions between connected cells and transfer of electrical signals In the present study we investigated the quantitative alterations of the GJ in the rat detrusor muscle and its functional changes related to the developing of unstable bladder （USB）. Methods Thirteen female Wistar rats （study group） with obstructive unstable bladder as determined by urodynamic study and 10 sham-operated rats （control group） were sacrificed at 6 weeks after surgery. Cystometric investigation, and the content and distribution of the GJ protein connexin 43 （Cx43） in the detrusors which were taken from the bladder of the rats were studied by Western blot and laser confocal microscopy with a double label immunohistochemistry technique. Results The expression of Cx43 was found adjacent to the detrusor with the laser confocal microscopy. The Cx43 expression increased markedly in the study group （pixel density 29.5±13.9, staining size （17.9±8.8） μm2） compared with the control group （pixel density 14.2±2.2, staining size （5.7±3.1） pm2, P 〈0.05）. Western blot analysis demonstrated that Cx43 in the study group （the average gray level was 31.066） was significantly higher than in the control group （the average gray level was 11.701, P 〈0.01 ）. Conclusion The increase of GJ leading to a intercellular excitatory communication is one of the important mechanisms related to developing unstable bladder.
