Mapping and measuring urban-rural inequalities in accessibility to social infrastructures

Equal access to social infrastructures is a fundamental prerequisite for sustainable development,but has long been a great challenge worldwide.Previous studies have primarily focused on the accessibility to social infras-tructures in urban areas across various scales,with less attention to rural areas,where inequality can be more severe.Particularly,few have investigated the disparities of accessibility to social infrastructures between urban and rural areas.Here,using the Changsha-Zhuzhou-Xiangtan urban agglomeration,China,as an example,we investigated the inequality of accessibility in both urban and rural areas,and further compared the urban-rural difference.Accessibility was measured by travel time of residents to infrastructures.We selected four types of social infrastructures including supermarkets,bus stops,primary schools,and health care,which were funda-mentally important to both urban and rural residents.We found large disparities in accessibility between urban and rural areas,ranging from 20 min to 2 h.Rural residents had to spend one to two more hours to bus stops than urban residents,and 20 min more to the other three types of infrastructures.Furthermore,accessibility to multiple infrastructures showed greater urban-rural differences.Rural residents in more than half of the towns had no access to any infrastructure within 15 min,while more than 60%of the urban residents could access to all infrastructures within 15 min.Our results revealed quantitative accessibility gap between urban and rural areas and underscored the necessity of social infrastructures planning to address such disparities.

Anticancer Activity of Rice Callus Suspension Cultures from Aromatic Varieties and Metabolites Regulated in Treated Cancer Cell Lines

Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.

MACS-W:A modified optical clearing agent for imaging 3D cell cultures

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

Spatial Distribution Pattern and Influencing Factors of Bed-and-breakfasts(B&Bs)from the Perspective of Urban-rural Differences:A Case Study of Jiaodong Peninsula,China

There are significant differences between urban and rural bed-and-breakfasts(B&Bs)in terms of customer positioning,economic strength and spatial carrier.Accurately identifying the differences in spatial characteristics and influencing factors of each type,is essential for creating urban and rural B&B agglomeration areas.This study used density-based spatial clustering of applications with noise(DBSCAN)and the multi-scale geographically weighted regression(MGWR)model to explore similarities and differences in the spatial distribution patterns and influencing factors for urban and rural B&Bs on the Jiaodong Peninsula of China from 2010 to 2022.The results showed that:1)both urban and rural B&Bs in Jiaodong Peninsula went through three stages:a slow start from 2010 to 2015,rapid development from 2015 to 2019,and hindered development from 2019 to 2022.However,urban B&Bs demonstrated a higher development speed and agglomeration intensity,leading to an increasingly evident trend of uneven development between the two sectors.2)The clustering scale of both urban and rural B&Bs continued to expand in terms of quantity and volume.Urban B&B clusters characterized by a limited number,but a higher likelihood of transitioning from low-level to high-level clusters.While the number of rural B&B clusters steadily increased over time,their clustering scale was comparatively lower than that of urban B&Bs,and they lacked the presence of high-level clustering.3)In terms of development direction,urban B&B clusters exhibited a relatively stable pattern and evolved into high-level clustering centers within the main urban areas.Conversely,rural B&Bs exhibited a more pronounced spatial diffusion effect,with clusters showing a trend of multi-center development along the coastline.4)Transport emerged as a common influencing factor for both urban and rural B&Bs,with the density of road network having the strongest explanatory power for their spatial distribution.In terms of differences,population agglomeration had a positive impact on the distribution of urban B&Bs and a negative effect on the distribution of rural B&Bs.Rural B&Bs clustering was more influenced by tourism resources compared with urban B&Bs,but increasing tourist stay duration remains an urgent issue to be addressed.The findings of this study could provide a more precise basis for government planning and management of urban and rural B&B agglomeration areas.

Preparation of lactic acid bacteria compound starter cultures based on pasting properties and its improvement of glutinous rice flour and dough

The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.

Spatial pattern of urban-rural integration in China and the impact of geography

Urban-rural integration (URI) is a global challenge that is highly related to inequalities, poverty, economic growth, and other Sustainable Development Goals (SDGs). Existing research has evaluated the extent of URI and explored its influencing factors, but urban-rural linkages are seldom incorporated in evaluation systems, and geographical factors are rarely recognized as the influencing factors. We construct a URI framework including regional economy, rural development, urban-rural linkage, and urban-rural gap. Based on a dataset consisting of 1,669 counties in China in 2020, we reveal the spatial pattern of URI and find a high correlation between the spatial pattern of URI and the relief degree of land surface (RDLS). Using structural equation modeling, we discover that topography has direct ( − 0.18, p < 0.001) and indirect ( − 0.17, p < 0.001) effects on URI. The indirect negative effects are mediated through the infrastructure, and the combination of localized advantages and modern technical conditions could mitigate the negative impact of topography. Finally, we identify 742 counties as lagging regions in URI, which can be clustered into eight types. Our findings could facilitate policy designing for those countries striving for integrated and sustainable development of urban and rural areas.

Spatiotemporal Evolution and Influencing Factors of Urban-rural Construction Land in Rural Industrialized Areas in China:Case Studies in Changyuan City and Xinxiang County of Henan Province

Since China’s reform and opening up in 1978,the acceleration of industrialization and urbanization in China had led to dramatic changes in the pattern of urban-rural land use.In this paper,we focused on the rural industrialized areas in central China(Xinxiang County and Changyuan City of Henan Province).We used the average nearest neighbor index,spatial statistical analysis,and a structural equation model to analyze the spatiotemporal evolution and influencing factors of urban-rural construction land based on multisource spatial data and survey data.The results showed that:1)from 1975 to 2019,the spatial distribution of urban-rural construction land in rural industrialized areas had evolved from homogeneous distribution to local agglomeration.In terms of comparative analysis of cases,the spatial distribution of urban-rural construction land in Changyuan City had shown a trend from diffusion to agglomeration,and Xinxiang County had overall shown a spatial change from homogenization to agglomeration and then to regional integration development.2)The hot spots with increased urban-rural construction land significantly expanded,and they had a high degree of spatial overlap with industrial development.Among them,Xinxiang County was concentrated in central and marginal areas,and Changyuan was mainly concentrated in central urban areas.3)From the evolution of spatial proximity of urban-rural construction land,rural industrialized areas generally decline,showing the characteristics of internal differentiation in the rate of change.4)Industrial development,social economy,the policy environment,and urban development played a positive role in promoting the expansion of urban-rural construction land in rural industrialized areas.To promote the optimal use of regional land and the integrated development of urban-rural areas,we should combine the advantages of regional endowment,formulate development strategies according to local conditions,and adjust the way that land is used in a timely manner.

A new approach towards the sustainability of urban-rural integration:The development strategy for central villages in the Abbasiya District of Iraq using GIS techniques

The development of rural areas usually has a positive impact on the urban-rural integration. This study explores an innovative approach to stimulate rural development by proposing qualified villages as central villages that can provide basic urban services for their residents and residents of neighboring villages. This approach can contribute to overcoming the various problems that rural areas faced at the social, economic, and environmental levels. It seeks to achieve spatial sustainability of rural areas, representing a new approach by integrating urban development methods to revitalize rural villages. The study analyzed 15 villages belonging to Abbasiya District of AL Kufa City in the Najaf Governorate of Iraq. Based on the GIS techniques and the analysis of urban service indicators(village population, percentage of urban building materials used in villages, distance between village and health centers, distance between village and main roads, nature of economic activity, distance between village and educational institutions, distance between village and drinking water sources, number of communication towers, and distance between village and urban administrative center), this study proposed that Abu Gharb and Albu Ghraib can served as central villages in Abbasiya District in the future. This study establishes a new rural spatial structure to achieve rural sustainable development, improves the development status of rural areas, and provides a theoretical basis for the government and relevant institutions to use urban service indicators, achieving rural sustainable development and formulating relevant development policies in Abbasiya District.

Thinking about Value Realization of Ecological Products under the Background of Urban-rural Integration in Chengdu“Western Control”Area

Chengdu's development strategy of“western control”has laid a solid ecological background for 8 western districts,cities and counties and cultivated good ecological resources.The integrated urban-rural development in the new era will realize the balanced development of urban and rural areas and give full play to the value of rural ecological resources through the“powerful combination”of urban and rural areas.To realize the urban-rural integration and high-quality rural development in Chengdu“western control”area,it is necessary to enhance the awareness of ecological resources and ecological products,and carry out unified planning of ecological infrastructure,ecological material products and ecological service products in the whole“western control”area.Meantime,ecological restoration must be carried out scientifically,and scene design for value transformation of ecological products should be implemented innovatively.

Inheritance and Innovation of Regional Cultures in Architectural Designs of Yichang

Against the background of outstanding traditional Chinese culture and the innovation-drivendevelopment strategy, this study analyzed regional cultures of Yichang where ancient Ba and Chu culturesmet and blended, proposed regional architectural designs adapted to modern conditions, fully clarified thedesign concepts of “deeply exploring, highly condensing, making the past serve the present, inheriting andinnovating” through analyzing the design cases in many regional architectural projects. The paper aimedat further inheriting and developing the outstanding traditional Chinese cultures, and provided theoreticalreferences for future designs.

Natural Disaster Risk Monitoring for Immovable Cultural Relics Based on Digital Twin

Natural disaster risk monitoring is an important task for disaster prevention and reduction.In the case of immovable cultural relics,however,the feedback mechanism,risk factors,monitoring logic,and monitoring indicators of natural disaster risk monitoring are complex.How to achieve intelligent perception and monitoring of natural disaster risk for immovable cultural relics has always been a focus and a challenge for researchers.Based on the analysis of the concepts and issues related to the natural disaster risk of immovable cultural relics,this paper proposes a framework for natural disaster risk monitoring for immovable cultural relics based on the digital twin.This framework focuses on risk monitoring,including the physical entities of natural disaster risk for immovable cultural relics,monitoring indicators,and virtual entity construction.A platform for monitoring the natural disaster risk of immovable cultural relics is proposed.Using the Puzhou Ancient City Site as a test bed,the proposed concept can be used for monitoring the natural disaster risk of immovable cultural relics at different scales.

Automatic Extraction Method of 3D Feature Guidelines for Complex Cultural Relic Surfaces Based on Point Cloud

Cultural relics line graphic serves as a crucial form of traditional artifact information documentation,which is a simple and intuitive product with low cost of displaying compared with 3D models.Dimensionality reduction is undoubtedly necessary for line drawings.However,most existing methods for artifact drawing rely on the principles of orthographic projection that always cannot avoid angle occlusion and data overlapping while the surface of cultural relics is complex.Therefore,conformal mapping was introduced as a dimensionality reduction way to compensate for the limitation of orthographic projection.Based on the given criteria for assessing surface complexity,this paper proposed a three-dimensional feature guideline extraction method for complex cultural relic surfaces.A 2D and 3D combined factor that measured the importance of points on describing surface features,vertex weight,was designed.Then the selection threshold for feature guideline extraction was determined based on the differences between vertex weight and shape index distributions.The feasibility and stability were verified through experiments conducted on real cultural relic surface data.Results demonstrated the ability of the method to address the challenges associated with the automatic generation of line drawings for complex surfaces.The extraction method and the obtained results will be useful for line graphic drawing,displaying and propaganda of cultural relics.

Comparison of Apigenin,Quercetin and Kaempferol Accumulation and Total Flavonoid Content in Leaves,Embryogenic Cultures and Cell Suspension Cultures of Parsley(Petroselinum crispum)

In recent years,there has been a growing interest in exploring alternative treatments for bone defects.Bone tissue engineering has turned its attention to plant extracts containing osteogenic flavonoids as potential promoters of bone regeneration.In our study,we specifically investigated the extract of Petroselinum crispum,a plant known for its abundance of osteogenic flavonoids such as apigenin,quercetin,and kaempferol.Our objective was to compare the total flavonoid content(TFC)and their accumulation in different sources.We obtained hydrolyzed aqueous extracts from the leaves of parsley plants(grown for 12 weeks in the field),weekly embryogenic cultures,and suspension cell cultures.The TFC,measured in mg quercetin equivalent per gram of dry weight(QE mg/g dw),was found to be 53.81±0.97 for 52 g dw of leaves(1 kg plant fw),31.10±1.52 for 420 g dw of embryogenic cultures,and 11.80±0.76 for 210 g dw of cell suspensions.Notably,only the leaves and embryogenic cultures showed significant accumulation of osteogenic flavonoids,with apigenin levels of 1.8±0.3 and 0.32±0.02 mg/g dw,and kaempferol levels of 1.0±0.18 and 0.2±0.01 mg/g dw,respectively.Our findings indicate that embryogenic cultures have the potential to produce up to 40%more apigenin in 12 weeks compared to 1 kg of parsley plant,thanks to their superior biomass reproductive competence.These results present a promising biotechnological approach for generating extracts enriched with bioactive compounds,which can be further utilized in in vitro osteogenic testing.

Construction of the "Three-integration" Rolling Development Mode in the Urban-rural Fringe from the Perspective of Thematic Business Engine : A Case Study of "Zhucheng Dinosaur Eco-city" in Shandong Province

Starting from the transformation and upgrading of traditional culture,the optimization and development of traditional industries,and the innovation of traditional models,this paper used cultural entrepreneurship to trigger the thematic business engine,and explored Zhucheng City with its unique dinosaur culture.It proposed"Three-integration"rolling development mode to build Zhucheng into a new highland of cultural entrepreneurship and create new competitiveness under the background of agricultural and rural modernization development.This paper also explored from the perspective of multi-subject collaboration of cultural entrepreneurship.Government needs to establish rolling development pilot projects and promote their application;enterprises need to play the engine role of thematic business through the mode of"getting larger to help smaller ones";the masses need to pay attention to the reaction of culture to the economy,so as to promote the wave of high-level promotion of cultural inheritance and urban-rural integration.

What Culture in European Cities?The Desperate Struggle of European Cultures against the American One

For a long time,American culture has been spreading and replacing other European cultures.This is a significant change.According to the French writer Régis Debray,images are replacing writing,the theme of happiness is replacing drama,and space is replacing time.Additionally,according to the German sociologist Max Weber,Protestantism has made Americans"ascetic inside the world,"meaning individuals are solely responsible for their own well-being and success in society.The idea of"collective grace"is suspect.In urban life,there is a void that is filled by tribalism,such as sports,music,and events.Unfortunately,in urban planning,public spaces for people to gather,like parks or public squares,are often neglected.Water and green spaces could be valuable resources in filling this void.However,there is a potential risk of private brands taking over placemaking.

A Comparative Study on the Bathing Cultures and the Spread of Plague Between China and the West-Taking the Spread of the Black Death in China and Europe in the 14th Century as an Example

An unprecedented catastrophe shrouded over the Chinese Empire ruled by the Yuan Dynasty and Medieval Europe-the Black Death during the 14th century with rapid spread,widespread impact,serious damage to property,and substantial deaths and injuries.In the aftermath of the Black Death,lifestyle changes were made and more sensible bathing habits evolved.In feudal China,Bathing Culture had variously adapted to each dynasty;while its existence in the West was heavily influenced by religion and other factors.In the post-pandemic era,this paper is dedicated to exploring the possible relevance of Bathing Cultures to the Black Death,and to conducting a comparative study of the plague spread in Yuan and Europe and its impact on ethnic Bathing Cultures,reckoning to present informative information to regular prevention and control of the following pandemics.

Investigation of the Acceptability of Cultured Meat in University Students

Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative.

Investigating the Creation of Cultural Atmospheres in Physical Bookstores Within the Context of Scene-Based Consumption:A Perspective Based on the Ways of Seeing

In an era of scene-based consumption,the essence of operating a bookstore lies in accepting and enabling the bookstore to play its role as a cultural medium for browsing and appreciating books,which in turn fosters the development of a distinctive bookstore culture.This new type of consumption context has led to an evolution in the book-reading culture in physical bookstores that is characterized by a shift from a paradigm of passive reading to one where the emphasis is on interactive viewing.This transition has laid the foundation for the creation of cultural atmospheres in bookstores,and it highlights the visual interactions that now exist between readers and books as well as with other related cultural industries.The dominant and fundamental logic behind this process is symbiosis,experience,aesthetics,immersion,and creativity.However,when a form of culture that focuses on consumption-oriented browsing begins to overshadow knowledge acquisition during the process of book reading,the cultural essence of bookstores is likely to be diminished.Therefore,the cultural essence within the scene-based consumption context should be enhanced by creating innovative viewing activities that showcase the cultural and emotional implications inherent in the scene itself,and thus help to align the identity of the bookstore with its in-situ cultural space.

Implementation of an international medical student pen-pal club:survey outcomes of perceptions in cultural competency and global health

Background:Pen-pal clubs(PPC)are used worldwide for students to learn about different cultures and other skillsets without the need for travel.Many medical students are interested in global health opportunities abroad but costs,scheduling,and other barriers allow few to participate in such experiences.It is important that medical students have nuanced global medical perspectives and can contribute to the global medical community.Objective:The purpose of this study is to demonstrate that an international medical student PPC improves medical students'perspectives of cultural competency and global health engagement.Methods:In 2021,a novel medical student PPC was established that began between an American and Japanese medical school.Following a shareholders meeting,it was decided that the number of medical schools involved globally be expanded through previous institutional affiliations and online presences.In total,the club connected 50 American medical students and 52 medical students from 17 high-and middle-income countries.The primary form of communication was online;pen-pals were encouraged to communicate monthly using provided topics,although frequency and way of communication was their discretion.In February 2022,American PPC members were emailed a qualitative survey to assess the PPC's impact.Results:The survey was completed by 42%of American PPC members,95%of which were 22-26 years.Participants were preclinical medical students,60%whom were female and the majority either white(47%)or Asian(43%).Overall,the PPC positively influenced American medical students'perception of global medicine,medical education,and their cultural competency after joining the PPC compared to prior(P=0.004).Conclusion:PPCs encourage medical students to think from a global perspective and foster open-mindedness within varying social and cultural contexts.Having a global communication platform for students during medical school education may be an additional way to train aspiring global leaders.

Historiography and Scientific Research of Religious Buildings of the Cucuteni-Trypillian Culture

The purpose of this article is to reveal the historiography and current state of research related to the cult buildings of the Eneolithic period of the Cucuteni-Trypillian cultural community.The article describes the historical path of archaeological discoveries,from the first Trypillian sanctuaries to the discovery of the largest Nebelivka temple complex in Ukraine in 2012.At the same time,the work partially raises the issue of religious beliefs of the ancient farmers of Central Europe in connection with the discovery of their sacred buildings.Since special scientific works have not comprehensively addressed this issue and are still fragmentary in nature,it is important at the present stage of research to make some coverage and systematization of existing materials on this issue.