目的评估尿液中性粒细胞明胶酶相关脂质运载蛋白（urinary neutrophil gelatinase—associated lipocalin，uNGAL）对危重病患者急性肾损伤（AKI）的早期诊断作用。方法选择上海市宝山区中西医结合医院ICU162例危重病患者，收集患者一...目的评估尿液中性粒细胞明胶酶相关脂质运载蛋白（urinary neutrophil gelatinase—associated lipocalin，uNGAL）对危重病患者急性肾损伤（AKI）的早期诊断作用。方法选择上海市宝山区中西医结合医院ICU162例危重病患者，收集患者一般情况、急性生理与慢性健康状况评分Ⅱ（acute physiology and chronic health evaluation，APACHEⅡ）及序贯器官衰竭估计评分（sequential organ failure assessment，SOFA），以及检测0、24、48h血肌酐（SCr）和uNGAL。比较分析AKI与非AKI患者指标水平，应用ROC曲线评价uNGAL对危重病患者AKI诊断的敏感度和特异度。结果AKI患者与非AKI患者uNGAL、SCr水平比较差异有统计学意义（P〈0．05）。参考2012改善全球肾脏病预后组织指南，将AKI患者分为三期，随着分期的升高，患者uNGAL与SOFA评分均呈上升趋势。uNGAL的ROC曲线下面积为0．838，敏感度与特异度均优于SCr。结论uNGAL有助于早期诊断AKI，可能对AKI严重程度提供帮助。展开更多
Objective: To study the value of serum NGAL and HE4 content change for diagnosing non-small cell lung cancer (NSCLC) and evaluating disease progression. Methods: Patients who were diagnosed with NSCLC and underwent su...Objective: To study the value of serum NGAL and HE4 content change for diagnosing non-small cell lung cancer (NSCLC) and evaluating disease progression. Methods: Patients who were diagnosed with NSCLC and underwent surgical resection in the First Affiliated Hospital of Chengdu Medical College between February 2015 and March 2017 were selected as the NSCLC group, and healthy volunteers who received physical examination over the same period were selected as control group. The NGAL and HE4 contents in serum of NSCLC group and control group as well as the expression of proliferation, apoptosis and invasion genes in NSCLC lesion of NSCLC group were detected. Results: Serum NGAL and HE4 levels of NSCLC group were significantly higher than those of control group, serum NGAL and HE4 levels of patients with TNM II and TNM III NSCLC were significantly higher than those of patients with TNM I NSCLC, and serum NGAL and HE4 levels of patients with TNM III NSCLC were significantly higher than those of patients with TNM II NSCLC;c-myc, Survivin, CatL, Vimentin, Slug and Snail mRNA expression in NSCLC lesion were significantly higher than those in adjacent lesion and positively correlated with serum NGAL and HE4 levels while PTEN, LATS1 and E-cadherin mRNA expression were significantly lower than those in adjacent lesion and negatively correlated with serum NGAL and HE4 levels. Conclusion: Abnormally elevated NGAL and HE4 in the serum of NSCLC patients can evaluate the proliferation and invasion of cancer cells to a certain extent.展开更多
文摘目的评估尿液中性粒细胞明胶酶相关脂质运载蛋白（urinary neutrophil gelatinase—associated lipocalin，uNGAL）对危重病患者急性肾损伤（AKI）的早期诊断作用。方法选择上海市宝山区中西医结合医院ICU162例危重病患者，收集患者一般情况、急性生理与慢性健康状况评分Ⅱ（acute physiology and chronic health evaluation，APACHEⅡ）及序贯器官衰竭估计评分（sequential organ failure assessment，SOFA），以及检测0、24、48h血肌酐（SCr）和uNGAL。比较分析AKI与非AKI患者指标水平，应用ROC曲线评价uNGAL对危重病患者AKI诊断的敏感度和特异度。结果AKI患者与非AKI患者uNGAL、SCr水平比较差异有统计学意义（P〈0．05）。参考2012改善全球肾脏病预后组织指南，将AKI患者分为三期，随着分期的升高，患者uNGAL与SOFA评分均呈上升趋势。uNGAL的ROC曲线下面积为0．838，敏感度与特异度均优于SCr。结论uNGAL有助于早期诊断AKI，可能对AKI严重程度提供帮助。
基金Youth Project of Natural Science Foundation of China(:81001345)Project of Science&Technology Department of Sichuan Province(:2014JY0039).
文摘Objective: To study the value of serum NGAL and HE4 content change for diagnosing non-small cell lung cancer (NSCLC) and evaluating disease progression. Methods: Patients who were diagnosed with NSCLC and underwent surgical resection in the First Affiliated Hospital of Chengdu Medical College between February 2015 and March 2017 were selected as the NSCLC group, and healthy volunteers who received physical examination over the same period were selected as control group. The NGAL and HE4 contents in serum of NSCLC group and control group as well as the expression of proliferation, apoptosis and invasion genes in NSCLC lesion of NSCLC group were detected. Results: Serum NGAL and HE4 levels of NSCLC group were significantly higher than those of control group, serum NGAL and HE4 levels of patients with TNM II and TNM III NSCLC were significantly higher than those of patients with TNM I NSCLC, and serum NGAL and HE4 levels of patients with TNM III NSCLC were significantly higher than those of patients with TNM II NSCLC;c-myc, Survivin, CatL, Vimentin, Slug and Snail mRNA expression in NSCLC lesion were significantly higher than those in adjacent lesion and positively correlated with serum NGAL and HE4 levels while PTEN, LATS1 and E-cadherin mRNA expression were significantly lower than those in adjacent lesion and negatively correlated with serum NGAL and HE4 levels. Conclusion: Abnormally elevated NGAL and HE4 in the serum of NSCLC patients can evaluate the proliferation and invasion of cancer cells to a certain extent.
