The anti-inflammatory effect and mechanism of Usnic acid （UA） were explored on lipopoly-saccharide （LPS）-stimulated RAW264.7 cell line.The effects of UA on pro-inflammatory cytokines including tumor necrosis facto...The anti-inflammatory effect and mechanism of Usnic acid （UA） were explored on lipopoly-saccharide （LPS）-stimulated RAW264.7 cell line.The effects of UA on pro-inflammatory cytokines including tumor necrosis factor-alfa （TNF-α）,interleukin-6 （IL-6） and interleukin-1 beta （IL-1β）,pro-inflammatory mediators such as nitric oxide （NO）,inducible nitric oxide synthase （iNOS） and cyclooxygenase-2 （COX-2） were studied by sandwich ELISA,real-time PCR and western blot analyses.Similarly,the effect of UA on anti-inflammatory cytokine interleukin-10 （IL-10） and anti-inflammatory mediator heme oxygenase-1 （HO-1） were also studied following the same methods.Furthermore,nuclear factor-κB （NF-κB） was assayed by immunocytochemistry.The results showed that UA has anti-inflammatory effect by down-regulatinng iNOS,COX-2,IL-1β,IL-6 and TNF-α,COX-2 gene expression through the suppression of NF-κB activation and increasing anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1 production.展开更多
Objective To investigate the molecular mechanisms that are responsible for anti-inflammatory effect of usnic acid (UA), the effects of UA from usnea longissm on tumor necrosis factor-α(TNF-α) and nitric oxide (NO) p...Objective To investigate the molecular mechanisms that are responsible for anti-inflammatory effect of usnic acid (UA), the effects of UA from usnea longissm on tumor necrosis factor-α(TNF-α) and nitric oxide (NO) production in peritoneal macrophages has been examined. Methods The different concentrations of UA were added to peritoneal macrophages. The TNF-α and NO production in peritoneal macrophages were examined with mouse TNF-α ELISA kit and NO content by measuring the amount of nitrite (NO-_2μmol/L) formed in the medium using Griess reaction. The activity of inducible nitric oxide synthase (i-NOS) was determined using i-NOS detection kit and the TNF-α mRNA expression was tested by reverse transcriptase polymerase chain reaction (RT-PCR). Results UA decreased the TNF-α and NO level in LPS-stimulated peritoneal macrophages in dose-dependent manner, the IC_ 50 values were 12.8μmol/L and 5.7μmol/L respectively. RT-PCR analysis indicated that UA could inhibit TNF-α mRNA expression; the activity analysis of i-NOS indicated that UA could inhibit the activity of i-NOS. Conclusion UA could inhibit the TNF-α and NO production in peritoneal macrophages, it may be associated with the anti-inflammatory activity of UA.展开更多
The normal fine structures of toxoplasma gondii tachyzoite and the erfect of usnic acid on the ultrastructure of the tachyzoites were observed by transmission electron microscope (TEM).The studies indicate that the pa...The normal fine structures of toxoplasma gondii tachyzoite and the erfect of usnic acid on the ultrastructure of the tachyzoites were observed by transmission electron microscope (TEM).The studies indicate that the pathological changes or the ultrastructure of the parasite took place under the effect of the 10 mg/L usnic acid for 30 min.These changes can be illustrated as folio'vs; 1)The posterior portion of the rhopties were destroyed.2)The membrane of the daughter cell fractured. 3)The membranate organellae or the organisms were demaged.展开更多
This paper reports the ultrastructural changes of trichomonas vaginaiis (T. vag. ) under the action of s-(-)usnic acid sodium in vitro. These changes can be shown by the following results:At first, the degranulation o...This paper reports the ultrastructural changes of trichomonas vaginaiis (T. vag. ) under the action of s-(-)usnic acid sodium in vitro. These changes can be shown by the following results:At first, the degranulation of the rough endoplasmic reticulum (RER) took place, the polyribosomes disaggregated. And then, the Golgi complex and the endoplasmic reticulum dilated. The cytoplasmic matrix presented and inhomogeneous apperance. Finally, the biomembrane loosed and fractured.The cell nuclei presented the karyorrhexis.展开更多
对近30年发表的松萝酸研究文献进行文献计量分析,探讨其当前研究现状、热点和发展趋势。检索Web of Science中1990—2021年发表的松萝酸相关研究文献,运用科学计量学工具VOSviewer1.6.17和CiteSpace 5.8.R3绘制松萝酸网络知识图谱,进行...对近30年发表的松萝酸研究文献进行文献计量分析,探讨其当前研究现状、热点和发展趋势。检索Web of Science中1990—2021年发表的松萝酸相关研究文献,运用科学计量学工具VOSviewer1.6.17和CiteSpace 5.8.R3绘制松萝酸网络知识图谱,进行国家、作者、关键词共现以及聚类分析,最终得到998篇外文文献纳入计量分析。松萝酸相关研究领域年度发文量逐年上升,表现为动态发展走高趋势,尤其是近15年一直维持较高的研究热度。全球76个国家的研究作者间形成多个紧密联系团队,中国在1990—2021年期间发表的外文文献数量排在第5位,且与15个国家在松萝酸研究中有合作。30年来,各国学者在天然来源、提取分离、药理活性及毒性方面较全面地进行了松萝酸的各项研究。近5年,相较于早期和中期研究阶段,松萝酸的研究热点发生了迁移,从来源、提取分离、抗炎抗菌、抗氧化活性等方面逐渐向抗肿瘤活性评价及机制研究、新型递送系统研究转变,较好地预测了松萝酸研究的热点和发展趋势,可为后续研究提供参考。展开更多
文摘The anti-inflammatory effect and mechanism of Usnic acid （UA） were explored on lipopoly-saccharide （LPS）-stimulated RAW264.7 cell line.The effects of UA on pro-inflammatory cytokines including tumor necrosis factor-alfa （TNF-α）,interleukin-6 （IL-6） and interleukin-1 beta （IL-1β）,pro-inflammatory mediators such as nitric oxide （NO）,inducible nitric oxide synthase （iNOS） and cyclooxygenase-2 （COX-2） were studied by sandwich ELISA,real-time PCR and western blot analyses.Similarly,the effect of UA on anti-inflammatory cytokine interleukin-10 （IL-10） and anti-inflammatory mediator heme oxygenase-1 （HO-1） were also studied following the same methods.Furthermore,nuclear factor-κB （NF-κB） was assayed by immunocytochemistry.The results showed that UA has anti-inflammatory effect by down-regulatinng iNOS,COX-2,IL-1β,IL-6 and TNF-α,COX-2 gene expression through the suppression of NF-κB activation and increasing anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1 production.
基金This work was supported by the Natural Science Foundation of Shaanxi Province ( No.2004G240) .
文摘Objective To investigate the molecular mechanisms that are responsible for anti-inflammatory effect of usnic acid (UA), the effects of UA from usnea longissm on tumor necrosis factor-α(TNF-α) and nitric oxide (NO) production in peritoneal macrophages has been examined. Methods The different concentrations of UA were added to peritoneal macrophages. The TNF-α and NO production in peritoneal macrophages were examined with mouse TNF-α ELISA kit and NO content by measuring the amount of nitrite (NO-_2μmol/L) formed in the medium using Griess reaction. The activity of inducible nitric oxide synthase (i-NOS) was determined using i-NOS detection kit and the TNF-α mRNA expression was tested by reverse transcriptase polymerase chain reaction (RT-PCR). Results UA decreased the TNF-α and NO level in LPS-stimulated peritoneal macrophages in dose-dependent manner, the IC_ 50 values were 12.8μmol/L and 5.7μmol/L respectively. RT-PCR analysis indicated that UA could inhibit TNF-α mRNA expression; the activity analysis of i-NOS indicated that UA could inhibit the activity of i-NOS. Conclusion UA could inhibit the TNF-α and NO production in peritoneal macrophages, it may be associated with the anti-inflammatory activity of UA.
文摘The normal fine structures of toxoplasma gondii tachyzoite and the erfect of usnic acid on the ultrastructure of the tachyzoites were observed by transmission electron microscope (TEM).The studies indicate that the pathological changes or the ultrastructure of the parasite took place under the effect of the 10 mg/L usnic acid for 30 min.These changes can be illustrated as folio'vs; 1)The posterior portion of the rhopties were destroyed.2)The membrane of the daughter cell fractured. 3)The membranate organellae or the organisms were demaged.
文摘This paper reports the ultrastructural changes of trichomonas vaginaiis (T. vag. ) under the action of s-(-)usnic acid sodium in vitro. These changes can be shown by the following results:At first, the degranulation of the rough endoplasmic reticulum (RER) took place, the polyribosomes disaggregated. And then, the Golgi complex and the endoplasmic reticulum dilated. The cytoplasmic matrix presented and inhomogeneous apperance. Finally, the biomembrane loosed and fractured.The cell nuclei presented the karyorrhexis.
文摘对近30年发表的松萝酸研究文献进行文献计量分析,探讨其当前研究现状、热点和发展趋势。检索Web of Science中1990—2021年发表的松萝酸相关研究文献,运用科学计量学工具VOSviewer1.6.17和CiteSpace 5.8.R3绘制松萝酸网络知识图谱,进行国家、作者、关键词共现以及聚类分析,最终得到998篇外文文献纳入计量分析。松萝酸相关研究领域年度发文量逐年上升,表现为动态发展走高趋势,尤其是近15年一直维持较高的研究热度。全球76个国家的研究作者间形成多个紧密联系团队,中国在1990—2021年期间发表的外文文献数量排在第5位,且与15个国家在松萝酸研究中有合作。30年来,各国学者在天然来源、提取分离、药理活性及毒性方面较全面地进行了松萝酸的各项研究。近5年,相较于早期和中期研究阶段,松萝酸的研究热点发生了迁移,从来源、提取分离、抗炎抗菌、抗氧化活性等方面逐渐向抗肿瘤活性评价及机制研究、新型递送系统研究转变,较好地预测了松萝酸研究的热点和发展趋势,可为后续研究提供参考。
