The endogenous fluorophores such as reduced nicotinarmide adenine dinucleotide(phosphate)(NAD(P)H)and favin adenine dinucleotide(FAD)in cells and tissues can be imaged by fuo-rescence lifetime imaging microscopy(FLIM)...The endogenous fluorophores such as reduced nicotinarmide adenine dinucleotide(phosphate)(NAD(P)H)and favin adenine dinucleotide(FAD)in cells and tissues can be imaged by fuo-rescence lifetime imaging microscopy(FLIM)to show the tisute morphology features,as well as the biomolecular changes in microenvironment.The two important coenzymes in cellular metabolism,NAD(P)H and FAD,can be used to monitor the cellular metabolic status.This work proposed a novel method to study the uterine metabolism at the adjacent site of healthy cervix.It was found that the benign uterine tumors such as leiomyomas and adenomyosis with abnormal cell growth can be detected by measuring the fluorescence lifetime of NAD(P)H and FAD in adjacent healthy cervical tisues.This method opened a novel strategy for alicted women to undergo the cervical biopsies instead of hysterectomies for detecting tumors,which can preserve the fertility of patients.The FLIM studying on NAD(P)H and FAD indicated the correlation between metabolism and some diseases,including diabetes,hyperthyroidism and obesity.It was also suggested that the metabolic level might be quite different for a patient with a malignant tumnor history.展开更多
基金supported by the National Natural Science Foundation of China(NSFC)(Grant Nos.11574056,61575046 and 11804350)Ministry of science and technology of the People's Republic of China(China-Serbia bilateral project SINO-SERBIA2018002)+2 种基金the Shanghai Sailing Program(Grant No.17YF1421300)Fudan University-CIOMP Joint Fund(FC2017-007,FC2018-001)Pioneering Project of Academy for Engineering and Technology,Fudan University(gyy2018-001,gyy2018-002).
文摘The endogenous fluorophores such as reduced nicotinarmide adenine dinucleotide(phosphate)(NAD(P)H)and favin adenine dinucleotide(FAD)in cells and tissues can be imaged by fuo-rescence lifetime imaging microscopy(FLIM)to show the tisute morphology features,as well as the biomolecular changes in microenvironment.The two important coenzymes in cellular metabolism,NAD(P)H and FAD,can be used to monitor the cellular metabolic status.This work proposed a novel method to study the uterine metabolism at the adjacent site of healthy cervix.It was found that the benign uterine tumors such as leiomyomas and adenomyosis with abnormal cell growth can be detected by measuring the fluorescence lifetime of NAD(P)H and FAD in adjacent healthy cervical tisues.This method opened a novel strategy for alicted women to undergo the cervical biopsies instead of hysterectomies for detecting tumors,which can preserve the fertility of patients.The FLIM studying on NAD(P)H and FAD indicated the correlation between metabolism and some diseases,including diabetes,hyperthyroidism and obesity.It was also suggested that the metabolic level might be quite different for a patient with a malignant tumnor history.
