[ Objective] This study aimed to optimize the extraction conditions of high-activity phytase from triticale. [ Method] Food and forage triticale 209 was used as an experimental material to investigate the optimal extr...[ Objective] This study aimed to optimize the extraction conditions of high-activity phytase from triticale. [ Method] Food and forage triticale 209 was used as an experimental material to investigate the optimal extraction conditions, including pH, solid-liquid ratio, extraction duration and active agent concentra- tion. The extracted phytase was purified with salting-out concentration method for SDS-PAGE eIectrophoresis Total protein content was measured using Bradford method; phytase activity was measured using vanadium ammonium molybdate method in accordance with the national standard GB/T 18634 -2009. [ Result] Phytase activity reached the highest under extraction conditions of pH 5.0, solid-liquid ratio 10, room temperature, shaking speed 200 r/min and shaking duration 1 h, without addition of active agems. [ Condusion] This study improved the extraction technology of phytase from wheat plants and was suitable for practical ap- plication.展开更多
基金Supported by Doctoral Scientific Research Start-up Fund of Harbin Normal University "Molecular Genetic Research of Black-grain Wheat Pigment"(08XBSK87)
文摘[ Objective] This study aimed to optimize the extraction conditions of high-activity phytase from triticale. [ Method] Food and forage triticale 209 was used as an experimental material to investigate the optimal extraction conditions, including pH, solid-liquid ratio, extraction duration and active agent concentra- tion. The extracted phytase was purified with salting-out concentration method for SDS-PAGE eIectrophoresis Total protein content was measured using Bradford method; phytase activity was measured using vanadium ammonium molybdate method in accordance with the national standard GB/T 18634 -2009. [ Result] Phytase activity reached the highest under extraction conditions of pH 5.0, solid-liquid ratio 10, room temperature, shaking speed 200 r/min and shaking duration 1 h, without addition of active agems. [ Condusion] This study improved the extraction technology of phytase from wheat plants and was suitable for practical ap- plication.
