[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the B.mori varieties suitable for the foreign gene expressi...[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the B.mori varieties suitable for the foreign gene expression.[Method] The recombinant B.mori baculovirus rBmNPV（P1-2A3C） are injected into pupae of original species and hybrids of B.mori respectively.Then,the expression of antigen is detected by ELISA method and compared.[Result] The expression level of P1-2A3C significantly differed in different B.mori varieties,in which,the hybrids of Qiufeng×TQ78 and Qiufeng×Sijiaojian could be considered as the effective bioreactors for high-level expression of foreign genes.[Conclusion] This study provided the basis for breeding special B.mori varieties which can highly express target protein of Asia I FMDV.展开更多
This study was carried out to find out how dependent are two local rice <span style="font-family:Verdana;">varieties (Magitolngar and Tox-728-1) to inoculation with selected</span><span style=...This study was carried out to find out how dependent are two local rice <span style="font-family:Verdana;">varieties (Magitolngar and Tox-728-1) to inoculation with selected</span><span style="font-family:Verdana;"> endogenous arbuscular mycorrhizal fungi (AMF) in a field where they were isolated. The multi-indigenous endomycorrhiza spores previously isolated and identified were the active ingredient in the production of bioinoculants used for this purpose. Spores massively multiplied from the rhizosphere of each rice variety in each of the four locally collected soils substrates were harvested to constitute 08 AMF inoculants (Kema = T1;Lama = T2;Latox = T3;Ndjatox = T4;Koloma = T5;Kolotox = T6;Ndjama = T7;Ketox = T8). These inoculants </span><span style="font-family:Verdana;">were field tested on the two rice varieties at Kelo, under a complete</span><span style="font-family:Verdana;"> randomized block design, comprising 10 treatments (8 inoculants, 01 positive control = T9, 01 negative control = T10), each of which was repeated thrice. The analysis of data indicates that AMF-inoculated plants were taller, developed more tillers/plant, and produced more rice grains/panicle than non-AMF-inoculated plants for both studied rice varieties. The rice variety Madjitolngar </span><span style="font-family:Verdana;">yielded more grains (7.5 t/ha) than the Tox-728-1 variety (5.8 t/ha).</span><span style="font-family:Verdana;"> Moreover, inoculants Koloma (T1), Latox (T3) and Kolotox (T6) on the one hand, Koloma (T1) and Ketox (T8) on the other hand, were best suited for the improvement of growth and yield of the rice varieties Madjitolngar and Tox-728-1 respectively, tested under field conditions at Kelo. In this study, </span><span style="font-family:Verdana;">the two rice varieties have shown a dependency to endomycorrhizal</span><span style="font-family:Verdana;"> symbiosis at Kelo, and therefore, an industrial-scale production of efficient endomycorrhal inoculants is necessary to sustainably boost the productivity of this important crop in Chad.</span>展开更多
The early season indica rice variety Zhong83-49 was developed in 1981 as one of theFbreeding lines by selections from IR24/Yuanfengzao(F)//Zhuke 2.After various tri-als and tests,Zhong 83-49 has been proved to be a
基金Supported by863Project for Development and Production of New FMDV accine for Livestock(2011AA10A211)Fund for Agricultural Since and Technology Achievements Transformation(2009GB23260437)~~
文摘[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the B.mori varieties suitable for the foreign gene expression.[Method] The recombinant B.mori baculovirus rBmNPV（P1-2A3C） are injected into pupae of original species and hybrids of B.mori respectively.Then,the expression of antigen is detected by ELISA method and compared.[Result] The expression level of P1-2A3C significantly differed in different B.mori varieties,in which,the hybrids of Qiufeng×TQ78 and Qiufeng×Sijiaojian could be considered as the effective bioreactors for high-level expression of foreign genes.[Conclusion] This study provided the basis for breeding special B.mori varieties which can highly express target protein of Asia I FMDV.
文摘This study was carried out to find out how dependent are two local rice <span style="font-family:Verdana;">varieties (Magitolngar and Tox-728-1) to inoculation with selected</span><span style="font-family:Verdana;"> endogenous arbuscular mycorrhizal fungi (AMF) in a field where they were isolated. The multi-indigenous endomycorrhiza spores previously isolated and identified were the active ingredient in the production of bioinoculants used for this purpose. Spores massively multiplied from the rhizosphere of each rice variety in each of the four locally collected soils substrates were harvested to constitute 08 AMF inoculants (Kema = T1;Lama = T2;Latox = T3;Ndjatox = T4;Koloma = T5;Kolotox = T6;Ndjama = T7;Ketox = T8). These inoculants </span><span style="font-family:Verdana;">were field tested on the two rice varieties at Kelo, under a complete</span><span style="font-family:Verdana;"> randomized block design, comprising 10 treatments (8 inoculants, 01 positive control = T9, 01 negative control = T10), each of which was repeated thrice. The analysis of data indicates that AMF-inoculated plants were taller, developed more tillers/plant, and produced more rice grains/panicle than non-AMF-inoculated plants for both studied rice varieties. The rice variety Madjitolngar </span><span style="font-family:Verdana;">yielded more grains (7.5 t/ha) than the Tox-728-1 variety (5.8 t/ha).</span><span style="font-family:Verdana;"> Moreover, inoculants Koloma (T1), Latox (T3) and Kolotox (T6) on the one hand, Koloma (T1) and Ketox (T8) on the other hand, were best suited for the improvement of growth and yield of the rice varieties Madjitolngar and Tox-728-1 respectively, tested under field conditions at Kelo. In this study, </span><span style="font-family:Verdana;">the two rice varieties have shown a dependency to endomycorrhizal</span><span style="font-family:Verdana;"> symbiosis at Kelo, and therefore, an industrial-scale production of efficient endomycorrhal inoculants is necessary to sustainably boost the productivity of this important crop in Chad.</span>
文摘The early season indica rice variety Zhong83-49 was developed in 1981 as one of theFbreeding lines by selections from IR24/Yuanfengzao(F)//Zhuke 2.After various tri-als and tests,Zhong 83-49 has been proved to be a
