AIM:To elucidate the role of vascular endothelial growth factor-165b(VEGF-165b)in blood-retinal barrier(BRB)injury in the rat acute glaucoma model.METHODS:In this study,the rat acute high intraocular pressure(HIOP)mod...AIM:To elucidate the role of vascular endothelial growth factor-165b(VEGF-165b)in blood-retinal barrier(BRB)injury in the rat acute glaucoma model.METHODS:In this study,the rat acute high intraocular pressure(HIOP)model was established before and after intravitreous injection of anti-VEGF-165b antibody.The expression of VEGF-165b and zonula occludens-1(ZO-1)in rat retina was detected by double immunofluorescence staining and Western blotting,and the breakdown of BRB was detected by Evans blue(EB)dye.RESULTS:The intact retina of rats expressed VEGF-165b and ZO-1 protein,which were mainly located in the retinal ganglion cell layer and the inner nuclear layer and were both co-expressed with vascular endothelial cell markers CD31.After acute HIOP,the expression of VEGF-165b was up-regulated;the expression of ZO-1 was down-regulated at 12h and then recovered at 3d;EB leakage increased,peaking at 12h.After intravitreous injection of anti-VEGF-165b antibody,the expression of VEGF-165b protein was no significantly changed;and the down-regulation of the expression of ZO-1 was more obvious;EB leakage became more serious,peaking at 3d.EB analysis also showed that EB leakage in the peripheral retina was greater than that in the central retina.CONCLUSION:The endogenous VEGF-165b protein may protect the BRB from acute HIOP by regulating the expression of ZO-1.The differential destruction of BRB after acute HIOP may be related to the selective loss of retinal ganglion cells.展开更多
Objective:To investigate the role of vascular endothelial growth factor(VEGF)165a,VEGF165b,and VEGF receptor(VEGFR)in the development of bovine follicles.Methods:We cultured follicular cells that were collected from s...Objective:To investigate the role of vascular endothelial growth factor(VEGF)165a,VEGF165b,and VEGF receptor(VEGFR)in the development of bovine follicles.Methods:We cultured follicular cells that were collected from small,medium,and large sized bovine follicles with estrogen and measured the expression of VEGF,VEGFR2 and VEGF165b by Western blot analysis and immunofluorescence.Results:The expression of VEGF165 increased in all follicle sizes and the expression of VEGF165b was increased in the small and large follicles after culturing in an estrogen containing medium.The expression of VEGFR2 was increased in the medium and large follicles after culturing with estrogen for 96 h.VEGF165 was activated at 100 ng/mL estrogen in the large follicles for 96 h.In addition,VEGFR2 was upregulated in the medium and large follicles after treated with 100 ng/mL estrogen for 96 h.Conclusions:This evidence suggests that the expression of VEGF165 and VEGFR is associated with estrogen stimulation during the development of bovine follicles and in an autocrine or paracrine manner.This reveals an advantage during oocyte maturation in vitro.展开更多
目的探讨先天性心脏病伴肺动脉高压患者血清B型尿钠肽(BNP)、血管内皮生长因子(VEGF)及转化生长因子-β_1(TGF-β_1)水平的表达及其临床意义。方法选择2014年1月至2017年6月深圳市人民医院收治的左向右分流型先天性心脏病患者86例作为...目的探讨先天性心脏病伴肺动脉高压患者血清B型尿钠肽(BNP)、血管内皮生长因子(VEGF)及转化生长因子-β_1(TGF-β_1)水平的表达及其临床意义。方法选择2014年1月至2017年6月深圳市人民医院收治的左向右分流型先天性心脏病患者86例作为研究组,按照肺动脉收缩压(PASP)分为A组(PASP≤30 mm Hg,n=20)、B组(30 mm Hg≤PASP<50 mm Hg,n=42)和C组(PASP≥50 mm Hg,n=24),另选取同期医院体检的健康人80例作为对照组。比较研究组和对照组、不同PASP分组患者的血清BNP、VEGF及TGF-β_1水平,并采用Pearson相关性分析分析其与PASP的相关性。结果研究组患者的血清BNP、VEGF及TGF-β_1水平分别为(236.76±75.43)pg/m L、(403.32±89.87)pg/m L、(837.53±102.25)pg/m L,均明显高于对照组的(88.36±26.73)pg/m L、(86.73±22.45)pg/m L、(128.81±32.13)pg/m L,差异均有统计学意义(P<0.05);患者血清BNP、VEGF及TGF-β_1水平随PASP升高而升高,不同PASP分组患者血清BNP、VEGF及TGF-β_1水平两两比较差异均有统计学意义(P<0.05);Pearson相关性分析结果显示,先天性心脏病伴肺动脉高压患者血清BNP、VEGF及TGF-β_1水平与PASP呈正相关(r=0.684、0.572、0.521,P<0.05),血清BNP、VEGF与TGF-β_1呈正相关(r=0.512、0.554,P<0.05)。结论左向右分流型先天性心脏病存在血清BNP、VEGF及TGF-β_1异常升高,三者与PASP呈正相关。展开更多
目的探讨弥漫性大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)Bcl-2蛋白的表达,血管内皮生长因子(vascular endothelial growth factor,VEGF)和微血管密度(microvessel density,MVD)的相关性,研究弥漫性大B细胞淋巴瘤Bcl-2蛋白...目的探讨弥漫性大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)Bcl-2蛋白的表达,血管内皮生长因子(vascular endothelial growth factor,VEGF)和微血管密度(microvessel density,MVD)的相关性,研究弥漫性大B细胞淋巴瘤Bcl-2蛋白与血管新生的关系。方法采用免疫组织化学染色方法(En Vison)检测弥漫性大B细胞淋巴瘤患者和对照组淋巴结组织中VEGF、Bcl-2、MVD表达情况。结果弥漫性大B细胞淋巴瘤患者Bcl-2蛋白、MVD、VEGF阳性表达均明显高于对照组,差异有统计学意义(P<0.01)。对三者进行两两相关性分析,结果显示MVD与VEGF表达呈正相关,Bcl-2蛋白阳性表达与VEGF表达呈正相关,Bcl-2蛋白阳性表达与MVD表达呈正相关。结论 VEGF的高表达可以促进肿瘤细胞增殖能力及侵袭能力,可促进Bcl-2蛋白高表达,同时加上本身促血管生成的作用,可降低弥漫性大B细胞淋巴瘤细胞凋亡,从而增加弥漫性大B细胞淋巴瘤的恶性程度,既而增加了弥漫性大B细胞淋巴瘤的治疗难度。展开更多
基金Supported by the National Natural Science Foundation of China(No.81660217)Youth Foundation of the First Affiliated Hospital of Hainan Medical University(No.HYYFYPY201922)。
文摘AIM:To elucidate the role of vascular endothelial growth factor-165b(VEGF-165b)in blood-retinal barrier(BRB)injury in the rat acute glaucoma model.METHODS:In this study,the rat acute high intraocular pressure(HIOP)model was established before and after intravitreous injection of anti-VEGF-165b antibody.The expression of VEGF-165b and zonula occludens-1(ZO-1)in rat retina was detected by double immunofluorescence staining and Western blotting,and the breakdown of BRB was detected by Evans blue(EB)dye.RESULTS:The intact retina of rats expressed VEGF-165b and ZO-1 protein,which were mainly located in the retinal ganglion cell layer and the inner nuclear layer and were both co-expressed with vascular endothelial cell markers CD31.After acute HIOP,the expression of VEGF-165b was up-regulated;the expression of ZO-1 was down-regulated at 12h and then recovered at 3d;EB leakage increased,peaking at 12h.After intravitreous injection of anti-VEGF-165b antibody,the expression of VEGF-165b protein was no significantly changed;and the down-regulation of the expression of ZO-1 was more obvious;EB leakage became more serious,peaking at 3d.EB analysis also showed that EB leakage in the peripheral retina was greater than that in the central retina.CONCLUSION:The endogenous VEGF-165b protein may protect the BRB from acute HIOP by regulating the expression of ZO-1.The differential destruction of BRB after acute HIOP may be related to the selective loss of retinal ganglion cells.
文摘Objective:To investigate the role of vascular endothelial growth factor(VEGF)165a,VEGF165b,and VEGF receptor(VEGFR)in the development of bovine follicles.Methods:We cultured follicular cells that were collected from small,medium,and large sized bovine follicles with estrogen and measured the expression of VEGF,VEGFR2 and VEGF165b by Western blot analysis and immunofluorescence.Results:The expression of VEGF165 increased in all follicle sizes and the expression of VEGF165b was increased in the small and large follicles after culturing in an estrogen containing medium.The expression of VEGFR2 was increased in the medium and large follicles after culturing with estrogen for 96 h.VEGF165 was activated at 100 ng/mL estrogen in the large follicles for 96 h.In addition,VEGFR2 was upregulated in the medium and large follicles after treated with 100 ng/mL estrogen for 96 h.Conclusions:This evidence suggests that the expression of VEGF165 and VEGFR is associated with estrogen stimulation during the development of bovine follicles and in an autocrine or paracrine manner.This reveals an advantage during oocyte maturation in vitro.
文摘目的探讨先天性心脏病伴肺动脉高压患者血清B型尿钠肽(BNP)、血管内皮生长因子(VEGF)及转化生长因子-β_1(TGF-β_1)水平的表达及其临床意义。方法选择2014年1月至2017年6月深圳市人民医院收治的左向右分流型先天性心脏病患者86例作为研究组,按照肺动脉收缩压(PASP)分为A组(PASP≤30 mm Hg,n=20)、B组(30 mm Hg≤PASP<50 mm Hg,n=42)和C组(PASP≥50 mm Hg,n=24),另选取同期医院体检的健康人80例作为对照组。比较研究组和对照组、不同PASP分组患者的血清BNP、VEGF及TGF-β_1水平,并采用Pearson相关性分析分析其与PASP的相关性。结果研究组患者的血清BNP、VEGF及TGF-β_1水平分别为(236.76±75.43)pg/m L、(403.32±89.87)pg/m L、(837.53±102.25)pg/m L,均明显高于对照组的(88.36±26.73)pg/m L、(86.73±22.45)pg/m L、(128.81±32.13)pg/m L,差异均有统计学意义(P<0.05);患者血清BNP、VEGF及TGF-β_1水平随PASP升高而升高,不同PASP分组患者血清BNP、VEGF及TGF-β_1水平两两比较差异均有统计学意义(P<0.05);Pearson相关性分析结果显示,先天性心脏病伴肺动脉高压患者血清BNP、VEGF及TGF-β_1水平与PASP呈正相关(r=0.684、0.572、0.521,P<0.05),血清BNP、VEGF与TGF-β_1呈正相关(r=0.512、0.554,P<0.05)。结论左向右分流型先天性心脏病存在血清BNP、VEGF及TGF-β_1异常升高,三者与PASP呈正相关。
