目的探讨三七总皂苷对动脉粥样硬化主动脉内皮的保护作用。方法载脂蛋白E基因敲除(apoE-KO)小鼠随机分为模型组、三七总皂苷组和辛伐他汀组,同种属C57BL/6J小鼠作为正常对照组。应用ELISA法测定小鼠血清氧化型低密度脂蛋白(oxLDL)含量,...目的探讨三七总皂苷对动脉粥样硬化主动脉内皮的保护作用。方法载脂蛋白E基因敲除(apoE-KO)小鼠随机分为模型组、三七总皂苷组和辛伐他汀组,同种属C57BL/6J小鼠作为正常对照组。应用ELISA法测定小鼠血清氧化型低密度脂蛋白(oxLDL)含量,透射电镜观察小鼠主动脉内皮细胞的超微结构。实时定量RT-PCR检测小鼠主动脉细胞分化抗原40(CD40)、血管细胞黏附分子-1(VCAM-1)mRNA表达水平。结果 apoE-KO小鼠的主动脉内皮和正常相比明显肿胀,连续性中断,可见细胞凋亡,胞核固缩,核内异染色质明显增多,线粒体空泡化。用药组内皮结构有不同程度的改善。与模型组相比,apoE-KO小鼠血清oxLDL水平显著升高(16.06ng/mL±4.16ng/mL vs 1.43ng/mL±0.84ng/mL,P<0.001),三七总皂苷及辛伐他汀均可显著降低apoE-KO小鼠血清oxLDL水平(4.45ng/mL±1.25ng/mL,3.92ng/mL±1.99ng/mL,P<0.001)。结论三七总皂苷对动脉粥样硬化小鼠血管内皮具有保护作用,其机制与降低血清oxLDL水平,下调主动脉CD40及VCAM-1的基因表达水平有关。展开更多
The tumor-associated antigen Ep-CAM (17-1A antigen), defined by the murine monoclonal antibody (mAb) 17-1A, has been identified as a 42-kD glycoprotein. The mAb 17-1A has been used for immunotherapy of colorectal can...The tumor-associated antigen Ep-CAM (17-1A antigen), defined by the murine monoclonal antibody (mAb) 17-1A, has been identified as a 42-kD glycoprotein. The mAb 17-1A has been used for immunotherapy of colorectal cancer. We obtained mAb 19F4 using a synthetic peptide containing antigen determinants of 17-1A antigen. The mAb 19F4 can bind the corresponding dominants of the 17-1A antigen in ELISA. Western-blot analysis demonstrated that mAb 19F4 recognized a 50-kD protein from cell lysates of MCF-7 (breast cancer cell line). Both mAb 19F4 and 17-1A detected a 42-kD protein in the cell lysates of HT-29 (colorectal cancer cell line). The results suggest that new members of the tumor-associated antigen family 17-1A may exist.展开更多
文摘目的探讨三七总皂苷对动脉粥样硬化主动脉内皮的保护作用。方法载脂蛋白E基因敲除(apoE-KO)小鼠随机分为模型组、三七总皂苷组和辛伐他汀组,同种属C57BL/6J小鼠作为正常对照组。应用ELISA法测定小鼠血清氧化型低密度脂蛋白(oxLDL)含量,透射电镜观察小鼠主动脉内皮细胞的超微结构。实时定量RT-PCR检测小鼠主动脉细胞分化抗原40(CD40)、血管细胞黏附分子-1(VCAM-1)mRNA表达水平。结果 apoE-KO小鼠的主动脉内皮和正常相比明显肿胀,连续性中断,可见细胞凋亡,胞核固缩,核内异染色质明显增多,线粒体空泡化。用药组内皮结构有不同程度的改善。与模型组相比,apoE-KO小鼠血清oxLDL水平显著升高(16.06ng/mL±4.16ng/mL vs 1.43ng/mL±0.84ng/mL,P<0.001),三七总皂苷及辛伐他汀均可显著降低apoE-KO小鼠血清oxLDL水平(4.45ng/mL±1.25ng/mL,3.92ng/mL±1.99ng/mL,P<0.001)。结论三七总皂苷对动脉粥样硬化小鼠血管内皮具有保护作用,其机制与降低血清oxLDL水平,下调主动脉CD40及VCAM-1的基因表达水平有关。
基金Supported by the National Key Basic Research SpecificFunds(No.G19990 75 60 7) and the National ScienceFoundation for Outstanding Young Scientist of China(No.3 0 0 2 5 0 3 8)
文摘The tumor-associated antigen Ep-CAM (17-1A antigen), defined by the murine monoclonal antibody (mAb) 17-1A, has been identified as a 42-kD glycoprotein. The mAb 17-1A has been used for immunotherapy of colorectal cancer. We obtained mAb 19F4 using a synthetic peptide containing antigen determinants of 17-1A antigen. The mAb 19F4 can bind the corresponding dominants of the 17-1A antigen in ELISA. Western-blot analysis demonstrated that mAb 19F4 recognized a 50-kD protein from cell lysates of MCF-7 (breast cancer cell line). Both mAb 19F4 and 17-1A detected a 42-kD protein in the cell lysates of HT-29 (colorectal cancer cell line). The results suggest that new members of the tumor-associated antigen family 17-1A may exist.
