The study aimed to investigate the effects of ivabradine on the levels of hypoxia-inducible factor-lalpha （HIF-1α） and VEGF in serum of rabbit with acute myocardial infarction （AMI）. Methods AMI model was establi...The study aimed to investigate the effects of ivabradine on the levels of hypoxia-inducible factor-lalpha （HIF-1α） and VEGF in serum of rabbit with acute myocardial infarction （AMI）. Methods AMI model was established by ligating the left anterior descending branch of the coronary artery in New Zealand white rabbits. Twenty five rabbits were randomly divided into 4 groups： sham-operated （S）, myocardial-infarction （M） with bisoprolol treatment （M ＋ B） and ivabradine-treated （I ＋ M）. The medical treatment began immediately after infarction and continued for 3 weeks. Serum of each rabbit was obtained at the following time points （24 h before the operation, 24 h, 3 d, 1 week, 2 weeks and 3 weeks after the operation）. ELISA was used to measure the levels of HIF-1α and VEGF of each sample. ECG and heart rates （before and after treatment） were analyzed. Results Baseline heart rate showed no significant differences between the 3 infarcted groups （M, M ＋ B, M ＋ I）. Three weeks later the heart rates were significantly lower in group M ＋ B and group M ＋ I than in group M. However, there was no statistic difference between the two drug-treated groups （P = 0.848）. The levels of HIF-1α and VEGF in groups M, M ＋ B and M ＋ I） increased significantly compared with group S （P 〈 0.01）. The productions of HIF-1α and VEGF were lower in group M ＋ B and group M ＋ I compared with group M （P 〈 0.01）. There was no statistical difference between the group M ＋ B and group M ＋ I （P 〉 0.05）, and the correlative analysis revealed that the production of HIF-1α was positively correlated with that of VEGF （r = 0.732, P 〈 0.01）. Conclusion Ivabradine can reduce heart rate and meanwhile decrease the serum levels of HIF-1α and VEGF after AMI.展开更多
文摘The study aimed to investigate the effects of ivabradine on the levels of hypoxia-inducible factor-lalpha （HIF-1α） and VEGF in serum of rabbit with acute myocardial infarction （AMI）. Methods AMI model was established by ligating the left anterior descending branch of the coronary artery in New Zealand white rabbits. Twenty five rabbits were randomly divided into 4 groups： sham-operated （S）, myocardial-infarction （M） with bisoprolol treatment （M ＋ B） and ivabradine-treated （I ＋ M）. The medical treatment began immediately after infarction and continued for 3 weeks. Serum of each rabbit was obtained at the following time points （24 h before the operation, 24 h, 3 d, 1 week, 2 weeks and 3 weeks after the operation）. ELISA was used to measure the levels of HIF-1α and VEGF of each sample. ECG and heart rates （before and after treatment） were analyzed. Results Baseline heart rate showed no significant differences between the 3 infarcted groups （M, M ＋ B, M ＋ I）. Three weeks later the heart rates were significantly lower in group M ＋ B and group M ＋ I than in group M. However, there was no statistic difference between the two drug-treated groups （P = 0.848）. The levels of HIF-1α and VEGF in groups M, M ＋ B and M ＋ I） increased significantly compared with group S （P 〈 0.01）. The productions of HIF-1α and VEGF were lower in group M ＋ B and group M ＋ I compared with group M （P 〈 0.01）. There was no statistical difference between the group M ＋ B and group M ＋ I （P 〉 0.05）, and the correlative analysis revealed that the production of HIF-1α was positively correlated with that of VEGF （r = 0.732, P 〈 0.01）. Conclusion Ivabradine can reduce heart rate and meanwhile decrease the serum levels of HIF-1α and VEGF after AMI.
