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A multilayer network diffusion-based model for reviewer recommendation
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作者 黄羿炜 徐舒琪 +1 位作者 蔡世民 吕琳媛 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第3期700-717,共18页
With the rapid growth of manuscript submissions,finding eligible reviewers for every submission has become a heavy task.Recommender systems are powerful tools developed in computer science and information science to d... With the rapid growth of manuscript submissions,finding eligible reviewers for every submission has become a heavy task.Recommender systems are powerful tools developed in computer science and information science to deal with this problem.However,most existing approaches resort to text mining techniques to match manuscripts with potential reviewers,which require high-quality textual information to perform well.In this paper,we propose a reviewer recommendation algorithm based on a network diffusion process on a scholar-paper multilayer network,with no requirement for textual information.The network incorporates the relationship of scholar-paper pairs,the collaboration among scholars,and the bibliographic coupling among papers.Experimental results show that our proposed algorithm outperforms other state-of-the-art recommendation methods that use graph random walk and matrix factorization and methods that use machine learning and natural language processing,with improvements of over 7.62%in recall,5.66%in hit rate,and 47.53%in ranking score.Our work sheds light on the effectiveness of multilayer network diffusion-based methods in the reviewer recommendation problem,which will help to facilitate the peer-review process and promote information retrieval research in other practical scenes. 展开更多
关键词 reviewer recommendation multilayer network network diffusion model recommender systems complex networks
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Diffusion mechanism simulation of cloud manufacturing complex network based on cooperative game theory 被引量:4
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作者 GENG Chao QU Shiyou +5 位作者 XIAO Yingying WANG Mei SHI Guoqiang LIN Tingyu XUE Junjie JIA Zhengxuan 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2018年第2期321-335,共15页
Cloud manufacturing is a specific implementation form of the "Internet + manufacturing" strategy. Why and how to develop cloud manufacturing platform(CMP), however, remains the key concern of both platform o... Cloud manufacturing is a specific implementation form of the "Internet + manufacturing" strategy. Why and how to develop cloud manufacturing platform(CMP), however, remains the key concern of both platform operators and users. A microscopic model is proposed to investigate advantages and diffusion forces of CMP through exploration of its diffusion process and mechanism. Specifically, a three-stage basic evolution process of CMP is innovatively proposed. Then, based on this basic process, a more complex CMP evolution model has been established in virtue of complex network theory, with five diffusion forces identified. Thereafter, simulations on CMP diffusion have been conducted. The results indicate that, CMP possesses better resource utilization,user satisfaction, and enterprise utility. Results of simulation on impacts of different diffusion forces show that both the time required for CMP to reach an equilibrium state and the final network size are affected simultaneously by the five diffusion forces. All these analyses indicate that CMP could create an open online cooperation environment and turns out to be an effective implementation of the "Internet + manufacturing" strategy. 展开更多
关键词 complex network cloud manufacturing innovation diffusion network effect Gale-Shapley algorithm cooperative game theory
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Simulating Some Complex Phenomena in Hydrothermal Ore-Forming Processes by Reaction-Diffusion CNN 被引量:1
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作者 Xu Deyi Yu Chongwen Bao Zhengyu Geochemistry Institute, China University of Geosciences, Wuhan 430074 《Journal of China University of Geosciences》 SCIE CSCD 2003年第3期215-219,共5页
Complexity phenomena like dynamic and static patterns, order from disorder, chaos and catastrophe were simulated by the application of 2-D reaction-diffusion CNN of two state variables and two diffusion coefficients t... Complexity phenomena like dynamic and static patterns, order from disorder, chaos and catastrophe were simulated by the application of 2-D reaction-diffusion CNN of two state variables and two diffusion coefficients transformed from Zhabotinksii model. They revealed somehow the mechanism of hydrothermal ore-forming processes, and answered several questions about the onset of ore forming. 展开更多
关键词 hydrothermal ore forming complexity reaction-diffusion CNN
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New complex variable meshless method for advection-diffusion problems 被引量:1
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作者 王健菲 程玉民 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第3期92-98,共7页
In this paper,an improved complex variable meshless method(ICVMM) for two-dimensional advection-diffusion problems is developed based on improved complex variable moving least-square(ICVMLS) approximation.The equi... In this paper,an improved complex variable meshless method(ICVMM) for two-dimensional advection-diffusion problems is developed based on improved complex variable moving least-square(ICVMLS) approximation.The equivalent functional of two-dimensional advection-diffusion problems is formed,the variation method is used to obtain the equation system,and the penalty method is employed to impose the essential boundary conditions.The difference method for twopoint boundary value problems is used to obtain the discrete equations.Then the corresponding formulas of the ICVMM for advection-diffusion problems are presented.Two numerical examples with different node distributions are used to validate and investigate the accuracy and efficiency of the new method in this paper.It is shown that ICVMM is very effective for advection-diffusion problems,and has a good convergent character,accuracy,and computational efficiency. 展开更多
关键词 meshless method improved complex variable moving least-square approximation improved complex variable meshless method advection-diffusion problem
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Relation between the complex Ginzburg-Landau equation and reaction-diffusion system
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作者 邵昕 任毅 欧阳颀 《Chinese Physics B》 SCIE EI CAS CSCD 2006年第3期513-517,共5页
The complex Ginzburg-Landau equation (CGLE) has been used to describe the travelling wave behaviour in reaction-diffusion (RD) systems. We argue that this description is valid only when the RD system is close to t... The complex Ginzburg-Landau equation (CGLE) has been used to describe the travelling wave behaviour in reaction-diffusion (RD) systems. We argue that this description is valid only when the RD system is close to the Hopf bifurcation, and is not valid when a RD system is away from the onset. To test this, we study spirals and anti-spirals in the chlorite-iodide-malonic acid (CIMA) reaction and the corresponding OGLE. Numerical simulations confirm that the OGLE can only be applied to the CIMA reaction when it is very near the Hopf onset. 展开更多
关键词 complex Ginzburg-Landau equation reaction-diffusion system chlorite-iodide-malonic acid
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Analysis of variable coefficient advection-diffusion problems via complex variable reproducing kernel particle method
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作者 翁云杰 程玉民 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第9期197-202,共6页
The complex variable reproducing kernel particle method (CVRKPM) of solving two-dimensional variable coefficient advection-diffusion problems is presented in this paper. The advantage of the CVRKPM is that the shape... The complex variable reproducing kernel particle method (CVRKPM) of solving two-dimensional variable coefficient advection-diffusion problems is presented in this paper. The advantage of the CVRKPM is that the shape function of a two-dimensional problem is formed with a one-dimensional basis function. The Galerkin weak form is employed to obtain the discretized system equation, and the penalty method is used to apply the essential boundary conditions. Then the corresponding formulae of the CVRKPM for two-dimensional variable coefficient advection-diffusion problems are obtained. Two numerical examples are given to show that the method in this paper has greater accuracy and computational efficiency than the conventional meshless method such as reproducing the kernel particle method (RKPM) and the element- free Galerkin (EFG) method. 展开更多
关键词 meshless method reproducing kernel particle method (RKPM) complex variable reproducingkernel particle method (CVRKPM) advection-diffusion problem
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Subtle role of latency for information diffusion in online social networks 被引量:4
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作者 熊菲 王夕萌 程军军 《Chinese Physics B》 SCIE EI CAS CSCD 2016年第10期587-595,共9页
Information diffusion in online social networks is induced by the event of forwarding information for users, and latency exists widely in user spreading behaviors. Little work has been done to reveal the effect of lat... Information diffusion in online social networks is induced by the event of forwarding information for users, and latency exists widely in user spreading behaviors. Little work has been done to reveal the effect of latency on the diffusion process. In this paper, we propose a propagation model in which nodes may suspend their spreading actions for a waiting period of stochastic length. These latent nodes may recover their activity again. Meanwhile, the mechanism of forwarding information is also introduced into the diffusion model. Mean-field analysis and numerical simulations indicate that our model has three nontrivial results. First, the spreading threshold does not correlate with latency in neither homogeneous nor heterogeneous networks, but depends on the spreading and refractory parameter. Furthermore, latency affects the diffusion process and changes the infection scale. A large or small latency parameter leads to a larger final diffusion extent, but the intrinsic dynamics is different. Large latency implies forwarding information rapidly, while small latency prevents nodes from dropping out of interactions. In addition, the betweenness is a better descriptor to identify influential nodes in the model with latency, compared with the coreness and degree. These results are helpful in understanding some collective phenomena of the diffusion process and taking measures to restrain a rumor in social networks. 展开更多
关键词 information diffusion node latency user behavior complex networks
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Temporal Analysis of the Diffusion of Knowledge in Networks of Software Maintenance and Development Project Team 被引量:3
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作者 Jorge Luiz dos Santos Renelson Ribeiro Sampaio 《Social Networking》 2019年第3期122-146,共25页
Different approaches have been established for applications of social and complex networks involving biological systems, passing through collaborative systems in knowledge networks and organizational systems. In this ... Different approaches have been established for applications of social and complex networks involving biological systems, passing through collaborative systems in knowledge networks and organizational systems. In this latter application, we highlight the studies focused on the diffusion of information and knowledge in networks. However, most of the time, the propagation of information in these networks and the resulting process of creation and diffusion of knowledge, have been studied from static perspectives. Additionally, the very concept of diffusion inevitably implies the inclusion of the temporal dimension, due to that it is an essentially dynamic process. Although static analysis provides an important perspective in structural terms, the behavioral view that reflects the evolution of the relationships of the members of these networks over time is best described by temporal networks. Thus, it is possible to analyze both the information flow and the structural changes that occur over time, which influences the dynamics of the creation and diffusion of knowledge. This article describes the computational modeling used to elucidate the creation and diffusion of knowledge in temporal networks formed to execute software maintenance and construction projects, for the period between 2007 and 2013, in the SERVI&#199;O FEDERAL DE PROCESSAMENTO DE DADOS (FEDERAL DATA PROCESSING SERVICE-SERPRO)—a public organization that provides information and communication technology services. The methodological approach adopted for the study was based on techniques for analyzing social and complex networks and on the complementary extensions that address temporal modeling of these networks. We present an exploratory longitudinal study that enabled a dynamic and structural analysis of the knowledge networks formed by members of software maintenance and development project teams between 2007 and 2013. The study enabled identification of knowledge categories throughout this period, in addition to the determination that the networks have a structure with small-world and scale-free models. Finally, we concluded that, in general, the topologies of the networks studies had characteristics for facilitating the flow of knowledge within the organization. 展开更多
关键词 KNOWLEDGE diffusion complex NETWORKS SOCIAL NETWORKS TEMPORAL NETWORKS
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Triple hit to the kidney-dual pathological crescentic glomerulonephritis and diffuse proliferative immune complexmediated glomerulonephritis: A case report
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作者 Dalia Ibrahim Sergey V Brodsky +2 位作者 Anjali A Satoskar Laura Biederman Natallia Maroz 《World Journal of Clinical Cases》 SCIE 2022年第32期11869-11876,共8页
BACKGROUND Anti-glomerular basement membrane(GBM)disease is a rare rapidly progressive glomerulonephritis,frequently associated with alveolar hemorrhage in the lungs and involving the kidney by crescentic glomerulonep... BACKGROUND Anti-glomerular basement membrane(GBM)disease is a rare rapidly progressive glomerulonephritis,frequently associated with alveolar hemorrhage in the lungs and involving the kidney by crescentic glomerulonephritis.It has been described in association with other glomerulonephritides[such as anti-neutrophilic antibody(ANCA)-glomerulonephritis,membranous nephropathy,and immunoglobulin(Ig)A nephropathy].CASE SUMMARY Herein we present an unusual case of concurrent anti-GBM disease,ANCAassociated crescentic glomerulonephritis and diffuse proliferative immune complex mediated glomerulonephritis with predominant staining for IgA and C3 by immunofluorescence.The patient is a 46-year-old Caucasian male who presented to the emergency department with acute onset of flank pain and was found to have high serum creatinine levels of 15 mg/dL,proteinuria,and hematuria.He rapidly deteriorated and became anuric.He was found to have high anti-GBM antibodies titers(151 units)and high anti-neutrophil cytoplasmic-ANCA.Despite prompt and early treatment,the patient’s condition worsened,and he succumbed to his illness.CONCLUSION Our case emphasizes the importance of a renal biopsy in anti-GBM disease,even in the presence of positive serum anti-GBM antibodies,to identify other potential causes of rapidly progressive glomerulonephritis.The challenge in treating such cases lies in the different therapy modalities. 展开更多
关键词 Anti-glomerular basement membrane disease Anti-neutrophilic antibody-associated glomerulonephritis diffuse proliferative glomerulone-phritis Immune complex mediated glomerulonephritis Case report
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Effect of humic acid contact time on the diffusion of Re(Ⅶ) in GMZ bentonite
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作者 王海 伍涛 +5 位作者 陈江 富宝锋 赵翔海 罗勇 赵耀林 贺朝会 《Nuclear Science and Techniques》 SCIE CAS CSCD 2015年第1期135-139,共5页
The effect of humic acid(HA) on the diffusion of Re(Ⅶ) was investigated in compacted Gaomiaozi(GMZ)bentonite by the through- and out- diffusion method. The effective diffusion coefficient, D e, and accessible porosit... The effect of humic acid(HA) on the diffusion of Re(Ⅶ) was investigated in compacted Gaomiaozi(GMZ)bentonite by the through- and out- diffusion method. The effective diffusion coefficient, D e, and accessible porosity, εacc, were measured in order to evaluate the impact of humic acid on Re(Ⅶ) diffusion. The D e value was in the range of(5.2–8.3)×10-12m2/s. The diffusion of Re(Ⅶ) was enhanced in the presence of HA, and the D e value increased with the increase of ageing time, indicating that the formation of HA-Re complexes was slow. Moreover, the εacc was in the range of(0.06– 0.16), which is less than the total porosity. It implies that the HA-Re complexes are anions. 展开更多
关键词 扩散法 膨润土 接触时间 腐植酸 有效扩散系数 总孔隙率 老化时间 腐殖酸
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A thermal flux-diffusing model for complex networks and its applications in community structure detection
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作者 沈毅 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第5期637-643,共7页
We introduce a thermal flux-diffusing model for complex networks. Based on this model, we propose a physical method to detect the communities in the complex networks. The method allows us to obtain the temperature dis... We introduce a thermal flux-diffusing model for complex networks. Based on this model, we propose a physical method to detect the communities in the complex networks. The method allows us to obtain the temperature distribution of nodes in time that scales linearly with the network size. Then, the local community enclosing a given node can be easily detected for the reason that the dense connections in the local communities lead to the temperatures of nodes in the same community being close to each other. The community structure of a network can be recursively detected by randomly choosing the nodes outside the detected local communities. In the experiments, we apply our method to a set of benchmarking networks with known pre-determined community structures. The experiment results show that our method has higher accuracy and precision than most existing globe methods and is better than the other existing local methods in the selection of the initial node. Finally. several real-world networks are investigated. 展开更多
关键词 complex networks community structure thermal flux-diffusing model
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住宅部品生产商质量认证行为扩散研究
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作者 苏义坤 于海楠 郑志哲 《土木工程与管理学报》 2024年第1期75-81,共7页
认证是提升住宅部品质量的有效手段,其扩散推广有利于住宅产业供给侧转型升级。为探究质量认证行为在住宅部品生产商间的扩散机理,本文引入复杂网络演化博弈理论构建了住宅部品质量认证扩散模型。通过数值仿真分析了市场导向和政府激励... 认证是提升住宅部品质量的有效手段,其扩散推广有利于住宅产业供给侧转型升级。为探究质量认证行为在住宅部品生产商间的扩散机理,本文引入复杂网络演化博弈理论构建了住宅部品质量认证扩散模型。通过数值仿真分析了市场导向和政府激励影响下质量认证行为在住宅部品生产商间的扩散过程。结果表明:消费者溢价、认证影响力与政府激励在一定范围内均可有效促进质量认证行为在住宅部品生产商间的正向扩散,其中生产商对消费者溢价的变化最为敏感。最后,通过分析住宅部品生产商质量认证行为扩散的演化特征,对质量认证在住宅部品生产领域的推广提出实质性对策与建议。研究从市场和政府两方面拓展了住宅部品生产商质量认证行为扩散的理论研究体系,促进了住宅高质量发展的理论实践。 展开更多
关键词 住宅部品 认证 复杂网络 演化博弈 扩散
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基于复杂网络演化博弈的可再生能源技术扩散仿真分析
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作者 张益岗 王小君 +3 位作者 孙庆凯 窦嘉铭 王英 刘曌 《电网技术》 EI CSCD 北大核心 2024年第4期1573-1582,I0051,I0052-I0054,共14页
“双碳”目标下,风光等可再生能源逐步替代煤等传统化石能源是实现电力系统能源转型的必然趋势,开展可再生能源技术扩散研究是模拟分析这一趋势的重要手段。然而,现有可再生能源技术扩散研究多从总体层面关注宏观扩散模型,忽略了发电商... “双碳”目标下,风光等可再生能源逐步替代煤等传统化石能源是实现电力系统能源转型的必然趋势,开展可再生能源技术扩散研究是模拟分析这一趋势的重要手段。然而,现有可再生能源技术扩散研究多从总体层面关注宏观扩散模型,忽略了发电商群体内部微观复杂博弈过程及相互作用对宏观扩散影响的复杂性。针对该问题,该文基于系统科学与博弈论视角,以发电商群体社会关系构建复杂网络,以不完全信息有限理性特征开展演化博弈。从发电商个体发电策略更新到发电商群体关系演化的逻辑,由点到面地分析可再生能源技术扩散的动态演化过程。基于两种典型复杂网络场景开展仿真,剖析发电商群体关系、政策标准以及市场价格等众多因素对可再生能源技术扩散演化的影响。算例结果既可为政府引导可再生能源技术在发电企业扩散方面提供理论支撑,亦可为演化博弈理论在能源行业中的广泛应用提供有益的借鉴。 展开更多
关键词 可再生能源 技术扩散 复杂网络 演化博弈
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基于复杂网络的电动汽车创新扩散博弈研究
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作者 孙威威 张峥 《复杂系统与复杂性科学》 CAS CSCD 北大核心 2024年第2期45-51,共7页
为了支持电动汽车企业通过协调复杂的多主体博弈关系推进创新扩散,构建了复杂网络的多主体博弈模型,阐明了影响创新扩散的关键变量。研究发现:制造商采纳不同技术的单位利润、专利费、技术优势系数是影响创新扩散的关键变量。节点度和... 为了支持电动汽车企业通过协调复杂的多主体博弈关系推进创新扩散,构建了复杂网络的多主体博弈模型,阐明了影响创新扩散的关键变量。研究发现:制造商采纳不同技术的单位利润、专利费、技术优势系数是影响创新扩散的关键变量。节点度和网络重连概率影响扩散速度,节点度越低则重连概率对扩散速度的影响越显著,且网络规模越高节点度和网络重连概率对扩散速度的影响越显著。最后,提出了推进电动汽车创新扩散的管理和政策启示。 展开更多
关键词 复杂网络 电动汽车 创新扩散 演化博弈
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基于FLEXPART模型的复杂地形大气扩散能力研究——以山西省孝义市和古县为例 被引量:1
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作者 岳江 马丽 +3 位作者 师莉红 王志伟 李雁宇 王小兰 《河南科学》 2024年第2期272-280,共9页
基于2012—2021年地面气象观测资料,采用无人机观测气温廓线,计算分别位于开阔盆地和狭长山谷地形的山西省孝义市和古县大气混合层厚度、大气自净能力指数、逆温层厚度;利用FLEXPART模型模拟两地不同时期(清洁期、重污染期)大气中颗粒... 基于2012—2021年地面气象观测资料,采用无人机观测气温廓线,计算分别位于开阔盆地和狭长山谷地形的山西省孝义市和古县大气混合层厚度、大气自净能力指数、逆温层厚度;利用FLEXPART模型模拟两地不同时期(清洁期、重污染期)大气中颗粒物传输路径及高度.结果表明:孝义市和古县大气自净能力指数分别为13.4和6.2,大气自净能力等级分别为二级和四级,春季大气自净能力最强,冬季最弱.冬季古县逆温强度大于孝义市,不利于大气污染物的扩散.清洁时期,两地污染物均沿西北方向传输,孝义市大气中粒子传输高度较高,传输距离更远;重污染期,两地空气中粒子传输高度均偏低,传输距离受地形影响,易造成当地大气污染.因此,开阔盆地相较于狭长山谷地区,大气污染物更易清除;开阔盆地大气污染物传输高度高于狭长山谷地区,且传输距离也更远. 展开更多
关键词 FLEXPART模型 复杂地形 大气扩散能力 大气自净能力 逆温
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中美知识融合网络与知识扩散网络对比
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作者 王雪莹 刘珊 +1 位作者 陈洪侃 步一 《情报杂志》 北大核心 2024年第5期167-175,166,共10页
[研究目的]当前借助引文关系开展知识流动规律分析的研究已经十分常见,但对于知识的融合与扩散过程的研究还不够深入。从复杂网络视角分析中美学者所创造知识的流动规律有助于厘清两地的科学生产力及知识流动的差异。[研究方法]通过引... [研究目的]当前借助引文关系开展知识流动规律分析的研究已经十分常见,但对于知识的融合与扩散过程的研究还不够深入。从复杂网络视角分析中美学者所创造知识的流动规律有助于厘清两地的科学生产力及知识流动的差异。[研究方法]通过引用关系构建知识融合级联网络和知识扩散级联网络,采用复杂网络分析法从网络数量特征、网络结构特征和级联网络国别等特征维度对中美知识融合网络和知识扩散网络进行对比分析。[研究结论]研究发现,纯美国学者组发表文献不论是知识融合级联网络还是知识扩散级联网络规模均更大,形成的知识融合级联网络密度、接近中心性和PageRank平均数更大。物理学相比于社会学,其文献的知识融合级联网络和知识扩散级联网络呈现更加稠密、节点联系更加紧密、中心性更强的结构。本研究有助于深化对知识融合与知识扩散的认识,加深对中美两国科研生产力差异的理解。 展开更多
关键词 科学文献 引文级联网络 复杂网络 知识融合 知识扩散 科学生产力 中国 美国
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社会互动视角下应急信息扩散的复杂网络演化研究
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作者 董昌其 刘纪达 张凯航 《中国安全生产科学技术》 CAS CSCD 北大核心 2024年第7期28-34,共7页
为揭示数字时代变革带来的灾害应急情景下,信息扩散呈现出社会主体交互的复杂规则特征,基于社会互动理论并运用复杂网络演化博弈方法,分别构建小世界和无标度2种属性特点的应急信息扩散网络结构;运用数值仿真建模,分析扩散收益、政府引... 为揭示数字时代变革带来的灾害应急情景下,信息扩散呈现出社会主体交互的复杂规则特征,基于社会互动理论并运用复杂网络演化博弈方法,分别构建小世界和无标度2种属性特点的应急信息扩散网络结构;运用数值仿真建模,分析扩散收益、政府引导、信息传播等因素对于不同社会互动规则网络的影响。研究结果表明:正面扩散以及互动溢出效应下的协同收益对主体积极行为激励明显,无标度互动结构的平均路径长与聚类系数低等特征会导致其在政府引导和收益激励下收敛速度均弱于小世界互动网络结构,但其幂律分布特性导致其在意见领袖舆论爆发时迅速影响微观主体行为,相关政府需要针对不同社会互动网络结构扩散特征采取相应引导策略加以调控。研究结果可为相关政府分类施策引导应急信息,使其正向有效扩散提供理论参考。 展开更多
关键词 社会互动 应急信息 复杂网络 演化动力学 信息扩散
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AP2M1抑制弥漫性大B细胞淋巴瘤细胞增殖和侵袭
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作者 李瑗春 严学倩 +3 位作者 范丹 及月茹 肖方 高静 《基础医学与临床》 2024年第3期308-316,共9页
目的探究接头相关蛋白质复合体2亚基μ1(AP2M1)对弥漫性大B细胞淋巴瘤(DLBCL)细胞增殖和侵袭的调控作用。方法将人弥漫性大B细胞淋巴瘤细胞系OCI-LY8分为对照组、NC-LV组、AP2M1-LV组。用Lipofectamine 2000进行细胞转染。四甲基偶氮唑... 目的探究接头相关蛋白质复合体2亚基μ1(AP2M1)对弥漫性大B细胞淋巴瘤(DLBCL)细胞增殖和侵袭的调控作用。方法将人弥漫性大B细胞淋巴瘤细胞系OCI-LY8分为对照组、NC-LV组、AP2M1-LV组。用Lipofectamine 2000进行细胞转染。四甲基偶氮唑盐(MTT)法检测细胞增殖,流式细胞仪检测细胞凋亡,Transwell小室法检测细胞迁移和侵袭。Western blot检测AP2M1、表皮生长因子受体(EGFR)、p-磷脂酰肌醇3激酶(PI3K)和p-蛋白质激酶B(AKT)蛋白表达。结果与对照组相比,AP2M1-shRNA组细胞中AP2M1的mRNA和蛋白相对表达量均降低(P<0.05),相对细胞活力升高(P<0.05),细胞凋亡率降低(P<0.05),迁移和侵袭细胞数量均升高(P<0.05);EGFR的蛋白相对表达量及PI3K和AKT的磷酸化水平均升高(P<0.05)。与对照组相比,AP2M1-LV组细胞中AP2M1的mRNA和蛋白相对表达量均升高(P<0.05),相对细胞活力降低(P<0.05),细胞凋亡率升高(P<0.05),迁移和侵袭细胞数量均降低(P<0.05),EGFR的蛋白相对表达量及PI3K和AKT的磷酸化水平均降低(P<0.05)。结论AP2M1的过表达部分通过抑制EGFR/PI3K/AKT信号通路来抑制DLBCL细胞的增殖和侵袭。 展开更多
关键词 弥漫性大B细胞淋巴瘤 接头相关蛋白质复合体2亚基μ1(AP2M1) 表皮生长因子受体 磷脂酰肌醇3激酶 蛋白质激酶B
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复杂地形下高危气体扩散后果影响的风洞实验研究
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作者 张杰东 《安全、健康和环境》 2024年第6期38-42,共5页
复杂地形对有毒有害气体扩散后果影响显著。研究了山区地形下的某集输站场发生事故泄漏时高含硫气体的运动规律,分别开展风速为1 m/s和2 m/s、8个风向条件下气体扩散分布规律环境风洞模拟实验,得到了不同位置的气体浓度等值线。结果表明... 复杂地形对有毒有害气体扩散后果影响显著。研究了山区地形下的某集输站场发生事故泄漏时高含硫气体的运动规律,分别开展风速为1 m/s和2 m/s、8个风向条件下气体扩散分布规律环境风洞模拟实验,得到了不同位置的气体浓度等值线。结果表明,高含硫气体在复杂地形下的近场扩散行为主要是由风场主导,而远场扩散后果则受地形影响明显。风洞试验可为石化行业高危气体泄漏扩散风险评估和应急响应提供可靠数据支撑。 展开更多
关键词 泄漏扩散 复杂地形 高危气体 风洞实验 浓度分布
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长链非编码RNA人类白细胞抗原复合体18通过微RNA-497-5p/细胞周期蛋白E1轴调节弥漫性大B细胞淋巴瘤的增殖、凋亡和侵袭
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作者 廖子龙 向国强 陈绘迦 《安徽医药》 CAS 2024年第4期794-799,I0006,共7页
目的 探讨长链非编码RNA人类白细胞抗原复合体18(lncRNA HCG18)调控微RNA-497-5p(miR-497-5p)/细胞周期蛋白E1(CCNE1)轴对弥漫性大B细胞淋巴瘤(DLBCL)细胞增殖、凋亡和侵袭的影响。方法 实时荧光定量PCR(qRT-PCR)、蛋白质印迹法分别检测... 目的 探讨长链非编码RNA人类白细胞抗原复合体18(lncRNA HCG18)调控微RNA-497-5p(miR-497-5p)/细胞周期蛋白E1(CCNE1)轴对弥漫性大B细胞淋巴瘤(DLBCL)细胞增殖、凋亡和侵袭的影响。方法 实时荧光定量PCR(qRT-PCR)、蛋白质印迹法分别检测2018年5月至2021年5月收集的恩施土家族苗族自治州中心医院DLBCL病人淋巴组织、良性淋巴结增生病人的淋巴组织、人正常B细胞永生化细胞HMy2.CIR、DLBCL细胞系SU-DHL-1、OCI-LY8、U2932中HCG18、miR-497-5p表达及CCNE1蛋白表达,将OCI-LY8细胞分为Ct组(正常培养的OCI-LY8细胞)、pcDNA组(细胞转染过表达物阴性对照)、pcDNA-HCG18组(细胞转染HCG18过表达物)、si-NC组(细胞转染小干扰RNA阴性对照)、si-HCG18组(细胞转染HCG18小干扰RNA)、si-HCG18+inhibitorNC组(细胞转染HCG18小干扰RNA和抑制物阴性对照)、si-HCG18+miR-497-5p inhibitor组(细胞转染HCG18小干扰RNA和miR-497-5p抑制物),CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡,Transwell检测细胞侵袭,蛋白质印迹法检测CCNE1、增殖细胞核抗原(PCNA)、Bcl-2相关X蛋白(Bax)、基质金属蛋白酶9(MMP-9)蛋白表达,双萤光素酶验证HCG18与miR-497-5p、miR-497-5p与CCNE1的关系。结果 在DLBCL淋巴组织和细胞中,HCG18、CCNE1蛋白高表达,miR-497-5p低表达,且在OCI-LY8细胞中HCG18、CCNE1蛋白表达上调最高,miR-497-5p表达下调最多(P<0.05),因此,以OCI-LY8细胞进行后续研究,与si-NC组比较,si-HCG18组HCG18(0.26±0.03比1.01±0.01)、CCNE1蛋白(0.45±0.03比1.44±0.19)表达降低,miR-497-5p(1.95±0.14比1.03±0.02)表达升高(P<0.05),与pcDNA组比较,pcDNA-HCG18组HCG18(1.96±0.23比1.02±0.01)、CCNE1蛋白(2.33±0.21比1.42±0.18)表达升高,miR-497-5p(0.28±0.02比1.02±0.02)表达降低(P<0.05),与siHCG18组、si-HCG18+inhibitor NC组比较,miR-497-5p表达降低(1.21±0.09比1.95±0.14、1.94±0.13),CCNE1蛋白(0.87±0.08比0.45±0.03、0.44±0.04)表达上调(P<0.05),沉默HCG18可抑制OCI-LY8细胞增殖、侵袭行为及PCNA、MMP-9蛋白表达,诱导细胞凋亡及Bax蛋白表达,而上调HCG18则呈相反趋势,下调miR-497-5p逆转了沉默HCG18对OCI-LY8细胞增殖、侵袭、凋亡的影响,HCG18靶向调控miR-497-5p/CCNE1。结论 沉默HCG18可能通过调控miR-497-5p/CCNE1抑制OCI-LY8细胞增殖、侵袭,诱导细胞凋亡。 展开更多
关键词 人类白细胞抗原复合体 细胞周期蛋白E 淋巴瘤 大B细胞 弥漫性 增殖细胞核抗原 BCL-2相关X蛋白质 基质金属蛋白酶9 微RNA-497-5p 增殖 侵袭
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