A method to identify complex Young＇s modulus of viscoelastic materials using forced longitudinal vibration of slender rods is proposed. The method differs from the beam one. Experimental tests were carried out at roo...A method to identify complex Young＇s modulus of viscoelastic materials using forced longitudinal vibration of slender rods is proposed. The method differs from the beam one. Experimental tests were carried out at room temperature with different lengths in 108 mm, 100 mm, 90 ram, 83.5 mm, 80 ram, 74.5 mm, 70 mm for the polycarbonate bars, and the curves of ratios A2/A1 between two ends of a viscoelastic bar versus frequencies are obtained, furthermore, the corresponding 3 dB bandwidth and the storage and loss modulus can be calculated. Sufficient number of obtained complex Young＇s modulus at different frequency allows us to calculate other ones using the least square method. If the step of the tested frequency is 5 Hz, the maximum error of results can be less than 6%. By comparison with the measurement methods which the previous literature mentioned, this new method simplifies the calculation, and the physical meaning appears apparently and clearly.展开更多
In this work, a method based on atomic force microscopy (AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic ...In this work, a method based on atomic force microscopy (AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic and viscoelastic properties of normal breast cells and cancerous breast cells were measured, showing significant differences in Young’s modulus and relaxation times between normal and cancerous breast cells. Remarkable differences in cellular topography between normal and cancerous breast cells were also revealed by AFM imaging. Next, the elastic and viscoelasitc properties of three other types of cell lines and primary normal B lymphocytes were measured; results demonstrated the potential of cellular viscoelastic properties in complementing cellular Young’s modulus for discerning different states of cells. This research provides a novel way to quantify the mechanical properties of cells by AFM, which allows investigation of the biomechanical behaviors of single cells from multiple aspects.展开更多
基金supported by the Fundamental Research Funds of China for the Central Universities(GK201001008)
文摘A method to identify complex Young＇s modulus of viscoelastic materials using forced longitudinal vibration of slender rods is proposed. The method differs from the beam one. Experimental tests were carried out at room temperature with different lengths in 108 mm, 100 mm, 90 ram, 83.5 mm, 80 ram, 74.5 mm, 70 mm for the polycarbonate bars, and the curves of ratios A2/A1 between two ends of a viscoelastic bar versus frequencies are obtained, furthermore, the corresponding 3 dB bandwidth and the storage and loss modulus can be calculated. Sufficient number of obtained complex Young＇s modulus at different frequency allows us to calculate other ones using the least square method. If the step of the tested frequency is 5 Hz, the maximum error of results can be less than 6%. By comparison with the measurement methods which the previous literature mentioned, this new method simplifies the calculation, and the physical meaning appears apparently and clearly.
基金supported by the National Natural Science Foundation of China (61503372, 61522312, U1613220, 61327014,61433017)the Youth Innovation Promotion Association CAS (2017243)the CAS FEA International Partnership Program for Creative Research Teams
文摘In this work, a method based on atomic force microscopy (AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic and viscoelastic properties of normal breast cells and cancerous breast cells were measured, showing significant differences in Young’s modulus and relaxation times between normal and cancerous breast cells. Remarkable differences in cellular topography between normal and cancerous breast cells were also revealed by AFM imaging. Next, the elastic and viscoelasitc properties of three other types of cell lines and primary normal B lymphocytes were measured; results demonstrated the potential of cellular viscoelastic properties in complementing cellular Young’s modulus for discerning different states of cells. This research provides a novel way to quantify the mechanical properties of cells by AFM, which allows investigation of the biomechanical behaviors of single cells from multiple aspects.
