The Effect of the Direct Anti-Human Globulin Test on the Clinical Outcome of Patients Receiving Blood Transfusion

Objective:To study the effect of the direct anti-human globulin test on the clinical efficacy of blood transfusion patients.Methods:52 transfused patients were selected for this study,of which 26 cases with positive direct anti-human globulin tests were included in the positive group,and another 26 cases with negative direct anti-human globulin tests were included in the negative group.The apparent efficacy of the patients in the two groups after blood transfusion was compared.Results:After blood transfusion,the apparent efficacy of the negative group was significantly higher,P<0.05;in the positive group,the proportion of the predominantly multi-antibody group was the highest;after blood transfusion,the post-transfusion apparent efficacy of the simple IgG group was higher than that of the multi-antibody group,P<0.05;comparing the intensity of the different antibodies resulted in the 1+group,and the 3+to 4+groups were significantly lower after blood transfusion,P<0.05.Conclusion:The use of the direct antiglobulin test in transfused patients showed that patients with positive results would have better clinical efficacy.Direct anti-human globulin tests will have an impact on the clinical efficacy of blood transfusion in patients with positive results,so it is very important to carry out a direct anti-human globulin test on blood transfusion patients.

微柱凝胶法在检测孕妇IgG效价中的应用研究

目的比较微柱凝胶法与试管抗人球蛋白试验法检测O型Rh D阳性孕妇血清中Ig G抗-A、抗-B效价的差异,并界定微柱凝胶法检测孕妇Ig G效价的阳性临界值。方法选择2012年8月～2014年9月血型为O型Rh D阳性孕妇血清标本360例,均进行微柱凝胶法与试管抗人球蛋白试验法检测Ig G抗-A、抗-B效价,比较两种检测方法的结果差异,确定微柱凝胶法检测孕妇Ig G效价的阳性临界值。结果微柱凝胶法测定的Ig G抗-A和Ig G抗-B效价几何均数为试管抗人球蛋白试验法测定的2倍;微柱凝胶法检测Ig G抗-A和Ig G抗-B的阳性率为72.8%和67.8%,显著高于试管抗人球蛋白试验法检测的阳性率（45.6%和34.4%）（P〈0.05）。微柱凝胶法与试管抗人球蛋白试验法测定Ig G抗-A和Ig G抗-B效价结果均呈高度正相关（rA=0.869,rB=0.912,P〈0.05）,根据回归方程计算微柱凝胶法具有临床意义的Ig G抗-A和Ig G抗-B效价的阳性临界值分别为128和121。结论微柱凝胶法检测孕妇Ig G效价较试管抗人球蛋白试验法更简便、快捷,灵敏度高。对其阳性临界值的界定,可为临床诊断提供可靠的依据。