Volatile components from Rhizoma Alpiniae Officinarum were respectively extracted by three methods including hydrodistillation, headspace solid-phase microextraction (HS-SPME) and diethyl ether extraction. A total o...Volatile components from Rhizoma Alpiniae Officinarum were respectively extracted by three methods including hydrodistillation, headspace solid-phase microextraction (HS-SPME) and diethyl ether extraction. A total of 40 (hydrodistillation), 32 (HS-SPME) and 37 (diethyl ether extraction) compounds were respectively identified by gas chromatography-mass spectrometry (GC/MS) and 22 compounds were overlapped, including β-farnesene, 7-muurolene, 2,6-dimethyl-6- (4-methyl-3-pentenyl)bicyclo[3.1.1]hept-2-ene, eucalyptol and cadina-1(10), 4-diene and so forth, varying in relative contents. HS-SPME is fast, sample saving and solvent-free and it also can achieve similar profiles as those from hydrodistillation and solvent extraction. Therefore, it can be the priority for extracting volatile components from medicinal plants.展开更多
This study evaluated the effects of worm infection on the volatile components,total phenolic compounds,and antioxidant capacities of Gomphidius rutilus.G.rutilus without worms(GW),G.rutilus infected by a small amount ...This study evaluated the effects of worm infection on the volatile components,total phenolic compounds,and antioxidant capacities of Gomphidius rutilus.G.rutilus without worms(GW),G.rutilus infected by a small amount of worms(GS;infected area<50%),and G.rutilus infected by a large amount of worms(GL;infected area>50%)were investigated.The volatile components of G.rutilus were analyzed by simultaneous distillation–extraction(SDE)and headspace solid-phase microextraction(HS-SPME)using gas chromatography–mass spectrometry(GC–MS).A total of 17 and 19 types of volatile compounds were detected,including ketones,alcohols,benzene,alkenes,aldehydes,esters,acids,and alkanes.Alcohols comprised the most abundant compound in GL,GS,and GW.The relative content of 1-octen-3-ol was the highest in all mushrooms.The concentration of eight-carbon(C8)compounds relative to the total volatile compounds varied widely,ranging from 40%(GW)to 64.34%(GS)and 84.42%(GS)and to 91.59%(GL),respectively,among the three samples.The antioxidant capability and the total phenolic contents of G.rutilus were evaluated in this study.The highest total phenolic content(TPC)of 192.23 mg GAE/g was found in GL,which differed significantly(P<0.05)from the latter two samples,whereas the lowest value of 156.11 mg GAE/g was found in GW.ABTS radical cation scavenging activity,FRAP ferric reducing antioxidant capacity(FRAP)radical scavenging activity,and oxygen radical absorbance capacity(ORAC)were investigated to screen the antioxidant properties of extracts.The contents of total phenolic compounds and their antioxidant capacities in vitro showed significant correlations(P<0.01).Among the three types of samples,the phenolic compounds of GL exhibited the highest antioxidant capacity,showing the values of 0.089 mM TE/g for ABTS,0.949 mM Fe^2+E/g for FRAP,and 1.952 M TE/g for ORAC.However,regarding the total antioxidant capacity,GS exhibited the highest antioxidant capacity,showing the values of 0.002648 mM TE/g for ABTS,0.004437 mM Fe^2+E/g for FRAP,and 0.256μM TE/g for ORAC.In conclusion,HS-SPME was more suitable for the extraction of volatile aroma components from G.rutilus.GL had the most abundant aroma components.GL had the highest TPC and antioxidant capacity compared with those of GS and GW,whereas GS showed the opposite results.Interestingly,GS was found to have the highest total antioxidant capacity in vitro.Based on these measured indicators,worm infection had no negative effect on the quality of G.rutilus.Therefore,worm-infected G.rutilus can also be consumed by humans.展开更多
The ultrasonic nebulization extraction(UNE) was developed and applied to the extraction of volatile components from traditional Chinese patent medicine Xiaoyao Pills. Several parameters of ultrasonic nebulization ex...The ultrasonic nebulization extraction(UNE) was developed and applied to the extraction of volatile components from traditional Chinese patent medicine Xiaoyao Pills. Several parameters of ultrasonic nebulization extraction including the sample particle size, solvent volume, extraction time and ultrasonic power were studied and selected. As a result, 2.4 g of sample with particle size of 80 mesh was extracted with 15 mL of n-hexane for 20 min at an ultrasonic power of 35 W. The volatile components were analyzed by gas chromatography-mass spectrometry (GC-MS) under the optimal conditions and 57 compounds were identified. The precision, repeatability and stability of the proposed method were also studied. Compared with ultrasonic-assisted extraction(UAE) and hydrodistillation(HD) extraction, the proposed method is more efficient, faster and easier to be operated at room temperature with smaller sample and energy consumption. It is suggested that the ultrasonic nebulization extraction can be used as a novel alternative method for the extraction of volatile components from traditional Chinese patent medicine.展开更多
Quality control of ginseng currently is mainly based on ginsenoside analysis,but rarely focuses on the volatile organic components.In the current work,an untargeted metabolomics approach,by headspace solid-phase micro...Quality control of ginseng currently is mainly based on ginsenoside analysis,but rarely focuses on the volatile organic components.In the current work,an untargeted metabolomics approach,by headspace solid-phase micro-extraction gas chromatography/mass spectrometry(HS-SPME-GC/MS),was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines,which included P.ginseng(PG),P.quinquefolius(PQ),P.notoginseng(PN),red ginseng(PGR),P.ginseng leaf(PGL),P.quinquefolius leaf(PQL),P.notoginseng leaf(PNL),P.ginseng flower(PGF),P.quinquefolius flower(PQF),P.notoginseng flower(PNF),P.japonicus(PJ),and P.japonicus var.major(PJvm).Chromatographic separation was performed on an HP-5MS elastic quartz capillary column using helium as the carrier gas,enabling good resolution within 1 h.We were able to characterize totally 259 volatile compounds,including 82 terpenes(T),46 alcohols(Alc),29 ketones(K),25 aldehydes(Ald),21 esters(E),and the others.By analyzing 90 batches of ginseng samples based on the untargeted metabolomics workflows,236 differential ions were unveiled,and accordingly 36 differential volatile components were discovered.It is the first report that simultaneously compares the compositional difference of volatile components among 12 Panax herbal medicines,and useful information is provided for the quality control of ginseng aside from the well-known ginsenosides.展开更多
[Objectives] To analyze volatile components of flowers and leaves of Thymus mongolicus. [Methods]Volatile components of collected T. mongolicus flower and leaves were separated by headspace solid-phase microextraction...[Objectives] To analyze volatile components of flowers and leaves of Thymus mongolicus. [Methods]Volatile components of collected T. mongolicus flower and leaves were separated by headspace solid-phase microextraction( SPME) and identified by gas chromatography/mass spectrometry( GC/MS) and normallized by peak area. [Results] A total of 24 and 14 compounds were identified from flowers and leaves of T. mongolicus in the total ion chromatogram,accounting for 99. 573% and 97. 187% of the total peak area,respectively. Main components of flowers and leaves of T. mongolicus include phenols and terpenes,and thymol accounts for 35. 38% and 49. 13% of flowers and leaves of T. mongolicus,respectively. [Conclusions] SPME-GC/MS can be applied in analyzing volatile components of flowers and leaves of T. mongolicus,to provide basis for further development and utilization of T. mongolicus.展开更多
<i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:""><span style="font-family:Verdana;"> Pall. var. <...<i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:""><span style="font-family:Verdana;"> Pall. var. </span><i><span style="font-family:Verdana;">trichocarpa </span></i><span style="font-family:Verdana;">is a variety of </span><i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:Verdana;"> Pall., and is currently the peony herb</span></span><span style="font-family:Verdana;">’</span><span style="font-family:""><span style="font-family:Verdana;">s principal cultivar group. Here, we study the differences in aromatic components and flowers of different varieties between two groups of cultivars, providing a reference for applying natural fragrance substances of peonies, breeding fragrant flower types, and developing and </span><span style="font-family:Verdana;">using improved varieties. Headspace solid-phase microextraction (HS-SPME),</span> <span style="font-family:Verdana;">gas chromatography-mass spectrometry (GC-MS), peak area normalization for</span><span style="font-family:Verdana;"> each component relative to content, component library (NIST14/NIST14S) retrieval, and a literature review were used to analyze the volatile compounds in flowers of eight peony varieties, such as </span></span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Gaoganhong</span><span style="font-family:Verdana;">”</span><span style="font-family:Verdana;">, and ten comospore peony varieties, such as </span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Jinshanhong</span><span style="font-family:Verdana;">”</span><span style="font-family:""><span style="font-family:Verdana;">. Results showed that the main volatile compound constituents in flowers of the two groups were terpenes and alcohols. Additionally, the content of eucalyptol, caryophyllene, α-Pinene, citronellol, and 3-Hexen-1-ol, acetate, (Z) was high. Peony cultivars contained linalool,</span><span style="font-family:Verdana;"> (1R)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene, and 1,4-dimethoxybenzene,</span><span style="font-family:Verdana;"> while comospore peony varieties contained 1,3,6-octatriene, 3,7-dimethyl-, (Z)-, phenylethyl alcohol, and geraniol. In this study, the differences between the volatile components of flowers of different peony varieties were clarified, laying a foundation for further molecular biology research into the floral fragrance of peonies and the cultivation of new varieties of aromatic peonies. At the same time, it also provides a theoretical basis for the development and application of peony flower by-products.展开更多
[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japon...[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japonica Houtt. were analyzed by the headspace solid-phase microextraction( HS-SPME) combined with GC-MS,and the relative percentage of each component was determined by peak area normalization. [Results] Thirty kinds of volatile components were identified from the leaves and rhizomes of P. japonica Houtt.,mainly including olefins,aromatic hydrocarbons,alkanes and esters. [Conclusions] The volatile components in different parts of P. japonica Houtt. were different,and there may be difference in the medicinal value of volatile components in different parts,thus it is required to take an overall consideration of these differences in the development and utilization of P. japonica Houtt.展开更多
[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether...[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether extract of P.chinense were analyzed and determined by gas chromatography.[Results]The volatile components of the methanol extract from P.chinense were the most,and phenolic acids and ketones accounted for a relatively high proportion,among which pyrogallic acid had the highest content.The ethyl acetate extract contained the second most volatile components,mostly acids and esters,of whichβ-sitosterol had the highest content;the chloroform and petroleum ether extracts had relatively few types of volatile components,most of which were alkanes,and the content ofγ-sitosterol shared by the two was the highest.The common substances of the four extracts were palmitic acid and phytol.[Conclusions]This study provides a theoretical basis for the quality evaluation of P.chinense and a scientific basis for its resource development.展开更多
[Objectives]This study was conducted to clarify the differences of volatile components in fresh fruit of Morinda citrifolia L.from different origins.[Methods]The method of HS-SPME-GC/MS detection was used to determine...[Objectives]This study was conducted to clarify the differences of volatile components in fresh fruit of Morinda citrifolia L.from different origins.[Methods]The method of HS-SPME-GC/MS detection was used to determine the volatile chemical components in fresh fruit of M.citrifolia.[Results]52,52 and 45 volatile components were identified from the fresh fruit of M.citrifolia from Xisha,Wanning and Haikou,respectively.Among them,the number and content of the identified esters were relatively high,mainly methyl caprylate,ethyl caprylate,4-pentenyl caproate,3-methylbuten-2-enyl caprylate,methyl caproate,and ethyl caproate.There were also fatty acids,alcohols,phenols,ketones,aldehydes and other substances.There were 33 common ingredients in the fruit from the three origins,mainly including caprylic acid,caproic acid,capric acid,methyl caprylate,ethyl caprylate,4-pentenyl caproate,3-methylbutene-2-enyl caprylate,methyl caproate,ethyl caproate,methyl caprate and hexyl caproate.[Conclusions]The types and contents of volatile components in fresh fruit of M.citrifolia from different origins were significantly different.展开更多
The amino acids and the volatile substances in grapes and wines play important roles in their quality,and the concentrations of these substances can be changed by how a vineyard is managed,e.g.,irrigation and fertigat...The amino acids and the volatile substances in grapes and wines play important roles in their quality,and the concentrations of these substances can be changed by how a vineyard is managed,e.g.,irrigation and fertigation regimes.This study aimed to evaluate the effect of fertilizer and water management on the distribution of amino acids,the volatile component profiles,and the sensory characteristics of Cabernet Sauvignon grapes and wines.The results showed that the amino acid concentration in grape berries was the highest under the 100%local fertilizer rate(HF)and 100%water irrigation quota(HW)treatment,and the volatile component concentration in wine was the highest under HF and 80%water irrigation quota(MW)treatment.The effect of irrigation on the amino acid content in grapes was greater than that of fertigation.The synergistic effect of fertilizer and water on arginine,serine,and glutamine in grape berries was significant.The interactive effect of fertigation and irrigation on the volatile substance in grapes was greater than that of fertigation and irrigation alone.The influence of irrigation on volatile substances in wines was greater than that of fertigation.In addition,there was also a correlation between the concentrations of multiple amino acids in grapes and volatile components in wines.Principal component analysis showed that the wine from the HFMW treatment had the best quality among all treatments.展开更多
Static headspace GC-MS method coupled with H/D exchange was firstly developed to determine and identify the volatile components in the fresh root and rhizome of Curcuma wenyujin. The TIC chromatograms of 3 batches of ...Static headspace GC-MS method coupled with H/D exchange was firstly developed to determine and identify the volatile components in the fresh root and rhizome of Curcuma wenyujin. The TIC chromatograms of 3 batches of fresh roots harvested at different time showed significant difference in the volatile components: the constitution was the same but the content of them was different. More than 60 volatile components in fresh roots (Root of C. wenyujin) and rhizomes (Rhizome of C. wenyujin) of C. wenyujin were detected, of which 51 and 48 volatile components were identified respectively. The fresh roots and rhizomes of C. wenyujin were found to have the similar volatile components. The contents of these components were calibrated by the response of β-elemene. In addition, the principal active component, β-elemene, was further confirmed and relatively quantified by its standard. γ-terpinene showed obvious allylic hydrogen/deuterium exchange using deuterium oxide which gave a new method to identify some compounds containing allylic hydrogen. At the same time, the active hydrogen compounds were also further confirmed. The results show that HS-GC-MS of volatile components from medical plants. method is a fast, simple and efficient way for the analysis展开更多
The objective was to investigate the effects of volatile components in Jiawei Wendan prescription on autistic behavior in rats,to analyze the volatile constituents and then to explore its effective components based on...The objective was to investigate the effects of volatile components in Jiawei Wendan prescription on autistic behavior in rats,to analyze the volatile constituents and then to explore its effective components based on the treatment of autism.The pregnant mice were divided into two groups according to the method that Schneider used.展开更多
The quality and flavor of scallops changes during the fresh dry rehydration process.In order to control the changes after rehydration,a scallop pretreatment method was adopted.The pretreatment method involved impregna...The quality and flavor of scallops changes during the fresh dry rehydration process.In order to control the changes after rehydration,a scallop pretreatment method was adopted.The pretreatment method involved impregnating fresh scallops with 0.2 g/100 m L konjac glucomannan(KGM),carrageenan(CA),and sodium alginate(SA)before the drying and rehydration process.After rehydration,the KGM treated samples had better water retention and the highest betaine content.The CA treated samples had a deepened yellow color and the highest content of flavoring amino acids(FAA)and inosine monophosphate(IMP).What’s more,a total of 77 volatile compounds were detected,with a maximum of 15 esters.The highest proportion in the four groups was eugenol,followed by n-hexadecanoic acid,methyl hexadecanoate etc.The SA group had no significant contribution in terms of quality and taste.The samples pretreated with KGM had the best quality,and those pretreated by CA had the best flavor.展开更多
[Objectives]This study was conducted to explore the dynamic changes of volatile flavor compounds in prepared pork during storage at different low-temperature conditions.[Methods]Prepared pork was stored at 4,-4 and-18...[Objectives]This study was conducted to explore the dynamic changes of volatile flavor compounds in prepared pork during storage at different low-temperature conditions.[Methods]Prepared pork was stored at 4,-4 and-18℃.The volatile flavor compounds of prepared pork were determined by solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS)at days 0,7,14,21 and 28,and relative odor activity value(OAV),principal component analysis(PCA)and cluster analysis(CA)were combined to analyze changes in volatile flavor compounds of prepared pork during storage.[Results]The total number of volatile flavor compounds gradually decreased with the prolongation of the storage period,and OAV analysis identified 22 key flavor compounds(OAV≥1).The results of PCA and CA showed that 2-methyl-1-butanol,1-octen-3-ol,linalool,cineole,hexanal and nonanal were the main key flavor components,and the degree of flavor degradation was low under both superchilling and freezing conditions.After 28 days of storage,the alcohol content in the chilling group was significantly higher than other two groups,and the overall content of volatile flavor compounds was also significantly higher than other two groups,indicating that the-4℃chilling storage was more favorable for maintaining the overall flavor of prepared pork.[Conclusions]This study provides a theoretical basis for finding a better storage method for prepared meat products.展开更多
To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis. A comprehensive two-dimensional gas chromatography (GCxGC) and high-resolution time-of-flight mass spectro...To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis. A comprehensive two-dimensional gas chromatography (GCxGC) and high-resolution time-of-flight mass spectrometry (HR-TOF/MS) with colorized fuzzy difference (CFD) method was used to investigate the effect of sulfur-fumigation on the volatile components from Radix Angelicae Sinensis. Twenty-five compounds that were found in sun-dried samples disappeared in sulfur-fumigated samples. Seventeen volatile components including two sulfur-containing compounds were newly generated for the first time in volatile oils of sulfur-fumigated Radix Angelicae Sinensis. The strategy can be successfully applied to rapidly and holistically discriminate sun-dried and sulfur-fumigated Radix Angelicae Sinensis. GCxGC-HR-TOF/MS based CFD is a powerful and feasible approach for the global quality evaluation of Radix Angelicae Sinensis as well as other herbal medicines.展开更多
A novel magnetic method using polystyrene modified magnetic nanoparticles to perform thermoheadspace extraction was successfully developed for extraction and preconcentration of volatile organic components in dry Trad...A novel magnetic method using polystyrene modified magnetic nanoparticles to perform thermoheadspace extraction was successfully developed for extraction and preconcentration of volatile organic components in dry Traditional Chinese Medicine(TCM) based on gas chromatography-ion trap/mass spectrometry with a Chromato Probe direct sample introduction device. The dried fruit of Amomum testaceum Ridl. was used as the object TCM. The optimum parameters of headspace magnetic solid-phase extraction were investigated, in which desorption solvent ethyl acetate played a key role in this method,and the headspace extraction temperature of 90℃ and the headspace extraction time of 15 min finally decided. Headspace solid-phase microextraction method was also used to analyze volatile compounds in the TCM to compare with the proposed method. The results show that 60 components were identified totally by two methods; most of the low boiling point chemical compounds are isolated by this new method. In this work, an environmental-friendly and cheap analytical method was established, and a new approach to analyze volatile compounds in dry Traditional Chinese Medicine was also provided.展开更多
In the current research,the use of a micromachined cantilever resonator as a platform for chemical gas sensing was examined.The microcantilever resonator integrates an electrothermal driving unit and a piezoresistive ...In the current research,the use of a micromachined cantilever resonator as a platform for chemical gas sensing was examined.The microcantilever resonator integrates an electrothermal driving unit and a piezoresistive detecting unit,and it is fabricated by direct bonding a silicon-on-insulator(SOI) wafer.With a particular polymer layer coated on the surface of the microcantilever,a gas sensor for volatile organic components(VOCs) detection can be realized.The operation mechanism provides the microcantilever resonator with integrated circuit(IC) compatibility in terms of both the fabrication process and operating voltage.The configuration of the microcantilever resonator can optimize the performance of the gas sensor.The SOI wafer provides a solution for the integrated fabrication of the microstructure,transducers,electronics,and the precise control of the resonator parameters.In this paper,the principles,design,analysis,process,and demonstration of the gas sensor based on the microcantilever resonator are presented.The experimental results provide confirmation that the polymer-coated microcantilever resonator has excellent performance with regard to VOC detection.展开更多
Banana/orange composite wine was brewed in the lab by liquid fermentation using angel yeast and lactic acid bacteria as the fermentation strains,and characterized by chemical analyses.It was found that the best ratio ...Banana/orange composite wine was brewed in the lab by liquid fermentation using angel yeast and lactic acid bacteria as the fermentation strains,and characterized by chemical analyses.It was found that the best ratio of banana juice to navel orange juice was 1:2,and the optimum alcohol fermentation parameters were as follows:28~30℃,22%initial sugar content,6%yeast,and 6 days of fermentation to reach an alcohol concentration of 11.63%v/v.The free amino acids in the composite wines were tested by automatic amino acid analyzer,and the flavor components of the composite wine were determined and analyzed by gas chromatography-mass spectrometry(GC-MS).Seven types of trace elements in the composite wine were measured by atomic absorption spectrometry.Results showed that there were 17 free amino acids,and their total concentration was up to 897.6 mg/L.A total of 16 key compounds were identified in the composite wine,11 of which were ester,4 of which were alcohols and 1 of which was acid.Magnesium,iron,copper and manganese elements were relatively rich in the wine,while lead was extremely low.展开更多
基金supported by the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No:2010B090400533)the International Scientific and Technological Cooperation Program of China (No:2009DFA31230)
文摘Volatile components from Rhizoma Alpiniae Officinarum were respectively extracted by three methods including hydrodistillation, headspace solid-phase microextraction (HS-SPME) and diethyl ether extraction. A total of 40 (hydrodistillation), 32 (HS-SPME) and 37 (diethyl ether extraction) compounds were respectively identified by gas chromatography-mass spectrometry (GC/MS) and 22 compounds were overlapped, including β-farnesene, 7-muurolene, 2,6-dimethyl-6- (4-methyl-3-pentenyl)bicyclo[3.1.1]hept-2-ene, eucalyptol and cadina-1(10), 4-diene and so forth, varying in relative contents. HS-SPME is fast, sample saving and solvent-free and it also can achieve similar profiles as those from hydrodistillation and solvent extraction. Therefore, it can be the priority for extracting volatile components from medicinal plants.
基金This work was supported financially by Edible mushroom resources exploitation and the key technology development in efficient processing,“National Key R&D Program of China”[Project No.2018YFD0400200]Liaoning Province,Shenyang Agricultural University,high-end talent introduction fund project[grant number SYAU20160003].
文摘This study evaluated the effects of worm infection on the volatile components,total phenolic compounds,and antioxidant capacities of Gomphidius rutilus.G.rutilus without worms(GW),G.rutilus infected by a small amount of worms(GS;infected area<50%),and G.rutilus infected by a large amount of worms(GL;infected area>50%)were investigated.The volatile components of G.rutilus were analyzed by simultaneous distillation–extraction(SDE)and headspace solid-phase microextraction(HS-SPME)using gas chromatography–mass spectrometry(GC–MS).A total of 17 and 19 types of volatile compounds were detected,including ketones,alcohols,benzene,alkenes,aldehydes,esters,acids,and alkanes.Alcohols comprised the most abundant compound in GL,GS,and GW.The relative content of 1-octen-3-ol was the highest in all mushrooms.The concentration of eight-carbon(C8)compounds relative to the total volatile compounds varied widely,ranging from 40%(GW)to 64.34%(GS)and 84.42%(GS)and to 91.59%(GL),respectively,among the three samples.The antioxidant capability and the total phenolic contents of G.rutilus were evaluated in this study.The highest total phenolic content(TPC)of 192.23 mg GAE/g was found in GL,which differed significantly(P<0.05)from the latter two samples,whereas the lowest value of 156.11 mg GAE/g was found in GW.ABTS radical cation scavenging activity,FRAP ferric reducing antioxidant capacity(FRAP)radical scavenging activity,and oxygen radical absorbance capacity(ORAC)were investigated to screen the antioxidant properties of extracts.The contents of total phenolic compounds and their antioxidant capacities in vitro showed significant correlations(P<0.01).Among the three types of samples,the phenolic compounds of GL exhibited the highest antioxidant capacity,showing the values of 0.089 mM TE/g for ABTS,0.949 mM Fe^2+E/g for FRAP,and 1.952 M TE/g for ORAC.However,regarding the total antioxidant capacity,GS exhibited the highest antioxidant capacity,showing the values of 0.002648 mM TE/g for ABTS,0.004437 mM Fe^2+E/g for FRAP,and 0.256μM TE/g for ORAC.In conclusion,HS-SPME was more suitable for the extraction of volatile aroma components from G.rutilus.GL had the most abundant aroma components.GL had the highest TPC and antioxidant capacity compared with those of GS and GW,whereas GS showed the opposite results.Interestingly,GS was found to have the highest total antioxidant capacity in vitro.Based on these measured indicators,worm infection had no negative effect on the quality of G.rutilus.Therefore,worm-infected G.rutilus can also be consumed by humans.
基金Supported by the Basic Research Foundation from Jilin University China(No.200903131)
文摘The ultrasonic nebulization extraction(UNE) was developed and applied to the extraction of volatile components from traditional Chinese patent medicine Xiaoyao Pills. Several parameters of ultrasonic nebulization extraction including the sample particle size, solvent volume, extraction time and ultrasonic power were studied and selected. As a result, 2.4 g of sample with particle size of 80 mesh was extracted with 15 mL of n-hexane for 20 min at an ultrasonic power of 35 W. The volatile components were analyzed by gas chromatography-mass spectrometry (GC-MS) under the optimal conditions and 57 compounds were identified. The precision, repeatability and stability of the proposed method were also studied. Compared with ultrasonic-assisted extraction(UAE) and hydrodistillation(HD) extraction, the proposed method is more efficient, faster and easier to be operated at room temperature with smaller sample and energy consumption. It is suggested that the ultrasonic nebulization extraction can be used as a novel alternative method for the extraction of volatile components from traditional Chinese patent medicine.
基金National Natural Science Foundation of China(Grant No.81872996)Natural Science Foundation of Tianjin of China(Grant No.20JCYBJC00060).
文摘Quality control of ginseng currently is mainly based on ginsenoside analysis,but rarely focuses on the volatile organic components.In the current work,an untargeted metabolomics approach,by headspace solid-phase micro-extraction gas chromatography/mass spectrometry(HS-SPME-GC/MS),was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines,which included P.ginseng(PG),P.quinquefolius(PQ),P.notoginseng(PN),red ginseng(PGR),P.ginseng leaf(PGL),P.quinquefolius leaf(PQL),P.notoginseng leaf(PNL),P.ginseng flower(PGF),P.quinquefolius flower(PQF),P.notoginseng flower(PNF),P.japonicus(PJ),and P.japonicus var.major(PJvm).Chromatographic separation was performed on an HP-5MS elastic quartz capillary column using helium as the carrier gas,enabling good resolution within 1 h.We were able to characterize totally 259 volatile compounds,including 82 terpenes(T),46 alcohols(Alc),29 ketones(K),25 aldehydes(Ald),21 esters(E),and the others.By analyzing 90 batches of ginseng samples based on the untargeted metabolomics workflows,236 differential ions were unveiled,and accordingly 36 differential volatile components were discovered.It is the first report that simultaneously compares the compositional difference of volatile components among 12 Panax herbal medicines,and useful information is provided for the quality control of ginseng aside from the well-known ginsenosides.
基金Supported by Natural Science Foundation Project of Inner Mongolia Autonomous Region(2015MS0324)Scientific Research Service Project of Chifeng University(KYFW-16-05)
文摘[Objectives] To analyze volatile components of flowers and leaves of Thymus mongolicus. [Methods]Volatile components of collected T. mongolicus flower and leaves were separated by headspace solid-phase microextraction( SPME) and identified by gas chromatography/mass spectrometry( GC/MS) and normallized by peak area. [Results] A total of 24 and 14 compounds were identified from flowers and leaves of T. mongolicus in the total ion chromatogram,accounting for 99. 573% and 97. 187% of the total peak area,respectively. Main components of flowers and leaves of T. mongolicus include phenols and terpenes,and thymol accounts for 35. 38% and 49. 13% of flowers and leaves of T. mongolicus,respectively. [Conclusions] SPME-GC/MS can be applied in analyzing volatile components of flowers and leaves of T. mongolicus,to provide basis for further development and utilization of T. mongolicus.
文摘<i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:""><span style="font-family:Verdana;"> Pall. var. </span><i><span style="font-family:Verdana;">trichocarpa </span></i><span style="font-family:Verdana;">is a variety of </span><i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:Verdana;"> Pall., and is currently the peony herb</span></span><span style="font-family:Verdana;">’</span><span style="font-family:""><span style="font-family:Verdana;">s principal cultivar group. Here, we study the differences in aromatic components and flowers of different varieties between two groups of cultivars, providing a reference for applying natural fragrance substances of peonies, breeding fragrant flower types, and developing and </span><span style="font-family:Verdana;">using improved varieties. Headspace solid-phase microextraction (HS-SPME),</span> <span style="font-family:Verdana;">gas chromatography-mass spectrometry (GC-MS), peak area normalization for</span><span style="font-family:Verdana;"> each component relative to content, component library (NIST14/NIST14S) retrieval, and a literature review were used to analyze the volatile compounds in flowers of eight peony varieties, such as </span></span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Gaoganhong</span><span style="font-family:Verdana;">”</span><span style="font-family:Verdana;">, and ten comospore peony varieties, such as </span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Jinshanhong</span><span style="font-family:Verdana;">”</span><span style="font-family:""><span style="font-family:Verdana;">. Results showed that the main volatile compound constituents in flowers of the two groups were terpenes and alcohols. Additionally, the content of eucalyptol, caryophyllene, α-Pinene, citronellol, and 3-Hexen-1-ol, acetate, (Z) was high. Peony cultivars contained linalool,</span><span style="font-family:Verdana;"> (1R)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene, and 1,4-dimethoxybenzene,</span><span style="font-family:Verdana;"> while comospore peony varieties contained 1,3,6-octatriene, 3,7-dimethyl-, (Z)-, phenylethyl alcohol, and geraniol. In this study, the differences between the volatile components of flowers of different peony varieties were clarified, laying a foundation for further molecular biology research into the floral fragrance of peonies and the cultivation of new varieties of aromatic peonies. At the same time, it also provides a theoretical basis for the development and application of peony flower by-products.
文摘[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japonica Houtt. were analyzed by the headspace solid-phase microextraction( HS-SPME) combined with GC-MS,and the relative percentage of each component was determined by peak area normalization. [Results] Thirty kinds of volatile components were identified from the leaves and rhizomes of P. japonica Houtt.,mainly including olefins,aromatic hydrocarbons,alkanes and esters. [Conclusions] The volatile components in different parts of P. japonica Houtt. were different,and there may be difference in the medicinal value of volatile components in different parts,thus it is required to take an overall consideration of these differences in the development and utilization of P. japonica Houtt.
基金Supported by Development of Huotanmu Capsules,a New Drug for Hepatitis B Treatment(20183046-1)Collaborative Innovation Center for Zhuang and Yao Medicines(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang and Yao Medicines(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Medicine(GJKY[2013]16)First-class Discipline in Guangxi of Traditional Chinese Pharmacology(GJKY[2018]12)。
文摘[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether extract of P.chinense were analyzed and determined by gas chromatography.[Results]The volatile components of the methanol extract from P.chinense were the most,and phenolic acids and ketones accounted for a relatively high proportion,among which pyrogallic acid had the highest content.The ethyl acetate extract contained the second most volatile components,mostly acids and esters,of whichβ-sitosterol had the highest content;the chloroform and petroleum ether extracts had relatively few types of volatile components,most of which were alkanes,and the content ofγ-sitosterol shared by the two was the highest.The common substances of the four extracts were palmitic acid and phytol.[Conclusions]This study provides a theoretical basis for the quality evaluation of P.chinense and a scientific basis for its resource development.
基金Supported by Hainan Province Basic and Applied Basic Research Program(Natural Science)High-level Talents(No.2019RC318)Nanfeng Special Phase III:Investigation and Protection of Cultivated Land and Fishery Water Resources(NFZX2021)the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(No.1630032022022)。
文摘[Objectives]This study was conducted to clarify the differences of volatile components in fresh fruit of Morinda citrifolia L.from different origins.[Methods]The method of HS-SPME-GC/MS detection was used to determine the volatile chemical components in fresh fruit of M.citrifolia.[Results]52,52 and 45 volatile components were identified from the fresh fruit of M.citrifolia from Xisha,Wanning and Haikou,respectively.Among them,the number and content of the identified esters were relatively high,mainly methyl caprylate,ethyl caprylate,4-pentenyl caproate,3-methylbuten-2-enyl caprylate,methyl caproate,and ethyl caproate.There were also fatty acids,alcohols,phenols,ketones,aldehydes and other substances.There were 33 common ingredients in the fruit from the three origins,mainly including caprylic acid,caproic acid,capric acid,methyl caprylate,ethyl caprylate,4-pentenyl caproate,3-methylbutene-2-enyl caprylate,methyl caproate,ethyl caproate,methyl caprate and hexyl caproate.[Conclusions]The types and contents of volatile components in fresh fruit of M.citrifolia from different origins were significantly different.
基金the S&T Program of Hebei,China(Grant No.20327001D)the leading Talent Project of Science and Technology Innovation in Ningxia Hui Autonomous Region(Grant No.2022GKLRLX07).
文摘The amino acids and the volatile substances in grapes and wines play important roles in their quality,and the concentrations of these substances can be changed by how a vineyard is managed,e.g.,irrigation and fertigation regimes.This study aimed to evaluate the effect of fertilizer and water management on the distribution of amino acids,the volatile component profiles,and the sensory characteristics of Cabernet Sauvignon grapes and wines.The results showed that the amino acid concentration in grape berries was the highest under the 100%local fertilizer rate(HF)and 100%water irrigation quota(HW)treatment,and the volatile component concentration in wine was the highest under HF and 80%water irrigation quota(MW)treatment.The effect of irrigation on the amino acid content in grapes was greater than that of fertigation.The synergistic effect of fertilizer and water on arginine,serine,and glutamine in grape berries was significant.The interactive effect of fertigation and irrigation on the volatile substance in grapes was greater than that of fertigation and irrigation alone.The influence of irrigation on volatile substances in wines was greater than that of fertigation.In addition,there was also a correlation between the concentrations of multiple amino acids in grapes and volatile components in wines.Principal component analysis showed that the wine from the HFMW treatment had the best quality among all treatments.
基金Project supported by the National Natural Science Foundation of China (No. 20475059) and the Chinese Academy of Sciences (No. KGCX2-SW-213-04).
文摘Static headspace GC-MS method coupled with H/D exchange was firstly developed to determine and identify the volatile components in the fresh root and rhizome of Curcuma wenyujin. The TIC chromatograms of 3 batches of fresh roots harvested at different time showed significant difference in the volatile components: the constitution was the same but the content of them was different. More than 60 volatile components in fresh roots (Root of C. wenyujin) and rhizomes (Rhizome of C. wenyujin) of C. wenyujin were detected, of which 51 and 48 volatile components were identified respectively. The fresh roots and rhizomes of C. wenyujin were found to have the similar volatile components. The contents of these components were calibrated by the response of β-elemene. In addition, the principal active component, β-elemene, was further confirmed and relatively quantified by its standard. γ-terpinene showed obvious allylic hydrogen/deuterium exchange using deuterium oxide which gave a new method to identify some compounds containing allylic hydrogen. At the same time, the active hydrogen compounds were also further confirmed. The results show that HS-GC-MS of volatile components from medical plants. method is a fast, simple and efficient way for the analysis
文摘The objective was to investigate the effects of volatile components in Jiawei Wendan prescription on autistic behavior in rats,to analyze the volatile constituents and then to explore its effective components based on the treatment of autism.The pregnant mice were divided into two groups according to the method that Schneider used.
基金funded by the National Key R&D Program of China(2020YFD0900900)。
文摘The quality and flavor of scallops changes during the fresh dry rehydration process.In order to control the changes after rehydration,a scallop pretreatment method was adopted.The pretreatment method involved impregnating fresh scallops with 0.2 g/100 m L konjac glucomannan(KGM),carrageenan(CA),and sodium alginate(SA)before the drying and rehydration process.After rehydration,the KGM treated samples had better water retention and the highest betaine content.The CA treated samples had a deepened yellow color and the highest content of flavoring amino acids(FAA)and inosine monophosphate(IMP).What’s more,a total of 77 volatile compounds were detected,with a maximum of 15 esters.The highest proportion in the four groups was eugenol,followed by n-hexadecanoic acid,methyl hexadecanoate etc.The SA group had no significant contribution in terms of quality and taste.The samples pretreated with KGM had the best quality,and those pretreated by CA had the best flavor.
基金Supported by Science and Technology Achievement Transformation Program of Sichuan Province(2023ZHCG0079)Research and Application of Key Techniques for Industrialization of Frozen Prepared Meat Dishes(GCZX22-35)Sichuan Pig Innovation Team of National Agricultural Industry Technology System(scsztd-2024-08-07).
文摘[Objectives]This study was conducted to explore the dynamic changes of volatile flavor compounds in prepared pork during storage at different low-temperature conditions.[Methods]Prepared pork was stored at 4,-4 and-18℃.The volatile flavor compounds of prepared pork were determined by solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS)at days 0,7,14,21 and 28,and relative odor activity value(OAV),principal component analysis(PCA)and cluster analysis(CA)were combined to analyze changes in volatile flavor compounds of prepared pork during storage.[Results]The total number of volatile flavor compounds gradually decreased with the prolongation of the storage period,and OAV analysis identified 22 key flavor compounds(OAV≥1).The results of PCA and CA showed that 2-methyl-1-butanol,1-octen-3-ol,linalool,cineole,hexanal and nonanal were the main key flavor components,and the degree of flavor degradation was low under both superchilling and freezing conditions.After 28 days of storage,the alcohol content in the chilling group was significantly higher than other two groups,and the overall content of volatile flavor compounds was also significantly higher than other two groups,indicating that the-4℃chilling storage was more favorable for maintaining the overall flavor of prepared pork.[Conclusions]This study provides a theoretical basis for finding a better storage method for prepared meat products.
基金Supported by the National Natural Science Foundation of China(No.81173546 and No.30940093)the Natural Science Foundation of Jiangsu Province,China(No.BK2009495)+4 种基金the International Science and Technology Cooperation Project of Jiangsu Province,China(No.BZ2011053)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD,No.2011ZYX2-001)the Postgraduate Research Innovation Project of Jiangsu Higher Education Institutions(No.KYLX_0975)the Chinese Medicine Research Program of Zhejiang Province,China(No.2014ZQ008)the Science Foundation of Zhejiang Chinese Medical University(No.2013ZZ12)
文摘To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis. A comprehensive two-dimensional gas chromatography (GCxGC) and high-resolution time-of-flight mass spectrometry (HR-TOF/MS) with colorized fuzzy difference (CFD) method was used to investigate the effect of sulfur-fumigation on the volatile components from Radix Angelicae Sinensis. Twenty-five compounds that were found in sun-dried samples disappeared in sulfur-fumigated samples. Seventeen volatile components including two sulfur-containing compounds were newly generated for the first time in volatile oils of sulfur-fumigated Radix Angelicae Sinensis. The strategy can be successfully applied to rapidly and holistically discriminate sun-dried and sulfur-fumigated Radix Angelicae Sinensis. GCxGC-HR-TOF/MS based CFD is a powerful and feasible approach for the global quality evaluation of Radix Angelicae Sinensis as well as other herbal medicines.
基金Support of this work by the Department of Education of Zhejiang Province (No. Pd2013016)Hangzhou Qianjiang Distinguished Experts Project (2014)+2 种基金the Science and Technology Department of Zhejiang Province (No. 2015C32006)Key Laboratory of Detection for Pesticide Residues of Ministry of Agriculture Projectthe Sprout Talented Project Program (No. 2011443)
文摘A novel magnetic method using polystyrene modified magnetic nanoparticles to perform thermoheadspace extraction was successfully developed for extraction and preconcentration of volatile organic components in dry Traditional Chinese Medicine(TCM) based on gas chromatography-ion trap/mass spectrometry with a Chromato Probe direct sample introduction device. The dried fruit of Amomum testaceum Ridl. was used as the object TCM. The optimum parameters of headspace magnetic solid-phase extraction were investigated, in which desorption solvent ethyl acetate played a key role in this method,and the headspace extraction temperature of 90℃ and the headspace extraction time of 15 min finally decided. Headspace solid-phase microextraction method was also used to analyze volatile compounds in the TCM to compare with the proposed method. The results show that 60 components were identified totally by two methods; most of the low boiling point chemical compounds are isolated by this new method. In this work, an environmental-friendly and cheap analytical method was established, and a new approach to analyze volatile compounds in dry Traditional Chinese Medicine was also provided.
基金Supported by the National Natural Science Foundation of China (No 50605040)
文摘In the current research,the use of a micromachined cantilever resonator as a platform for chemical gas sensing was examined.The microcantilever resonator integrates an electrothermal driving unit and a piezoresistive detecting unit,and it is fabricated by direct bonding a silicon-on-insulator(SOI) wafer.With a particular polymer layer coated on the surface of the microcantilever,a gas sensor for volatile organic components(VOCs) detection can be realized.The operation mechanism provides the microcantilever resonator with integrated circuit(IC) compatibility in terms of both the fabrication process and operating voltage.The configuration of the microcantilever resonator can optimize the performance of the gas sensor.The SOI wafer provides a solution for the integrated fabrication of the microstructure,transducers,electronics,and the precise control of the resonator parameters.In this paper,the principles,design,analysis,process,and demonstration of the gas sensor based on the microcantilever resonator are presented.The experimental results provide confirmation that the polymer-coated microcantilever resonator has excellent performance with regard to VOC detection.
基金support from the Open fund of fruit and vegetable processing and storage engineering technology development center of Guangdong College (GrantNo.2015B003).
文摘Banana/orange composite wine was brewed in the lab by liquid fermentation using angel yeast and lactic acid bacteria as the fermentation strains,and characterized by chemical analyses.It was found that the best ratio of banana juice to navel orange juice was 1:2,and the optimum alcohol fermentation parameters were as follows:28~30℃,22%initial sugar content,6%yeast,and 6 days of fermentation to reach an alcohol concentration of 11.63%v/v.The free amino acids in the composite wines were tested by automatic amino acid analyzer,and the flavor components of the composite wine were determined and analyzed by gas chromatography-mass spectrometry(GC-MS).Seven types of trace elements in the composite wine were measured by atomic absorption spectrometry.Results showed that there were 17 free amino acids,and their total concentration was up to 897.6 mg/L.A total of 16 key compounds were identified in the composite wine,11 of which were ester,4 of which were alcohols and 1 of which was acid.Magnesium,iron,copper and manganese elements were relatively rich in the wine,while lead was extremely low.