GC-MS/MS Method for Determination and Pharmacokinetics of Main Components of Cang-Ai Volatile Oil in Rat Plasma after Intravenous Administration

[Objectives]To establish a gas chromatography-triple quadrupole mass spectrometry(GC-MS/MS)method based on multiple reaction monitoring(MRM)mode for the analysis of the major components in Cang-ai volatile oil(CAVO).[Methods]An ultrasensitive gas chromatography-tandem mass spectrometry(GC-MS/MS)method was developed and validated for the determination of three highly abundant components in rat plasma samples.Paeonol was used as an internal standard.A multiple reaction monitoring(MRM)model was employed for the quantification of the three major components of CAVO.[Results]The method demonstrated linearity over the range of 0.25 to 50μg/mL with a correlation coefficient(R 2)greater than 0.9998.The lower limit of quantification was 0.25μg/mL.Intra-day and inter-day accuracy and precision were within 15%.Extraction recovery and matrix effect values ranged from 90.1%to 110.6%and 0.1%to 2.1%,respectively.[Conclusions]This method was successfully applied to the simultaneous determination of the three components in high-level CAVO plasma samples,providing a basis for subsequent studies of CAVO.

Chemical component analysis of volatile oil in drug pair Herba Ephedrae-Ramulus Cinnamomi by GC-MS and CRM

Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volume integration.By means of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.In total,97,62,and 78 volatile chemical components in volatile oil of HE,RC,and DP HE-RC,were respectively determined qualitatively and quantitatively,accounting for 90.08%,91.62%,and 89.76% total contents of volatile oil of HE,RC,and DP HE-RC respectively.It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo[4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol and(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.

GC-MS Analysis of Chemical Components of Volatile Oil in Different Parts of Fennel( Foenicuzu vulgare Mill. )

[Objectives] This study was conducted to analyze the composition of volatile oil in different parts of fennel(Foenicuzu vulgare Mill.) and to compare the differences in the composition of volatile oil in different parts of fennel.[Methods]The steam distillation method was applied to extract volatile oil from different parts of fennel,and the components of volatile oil from different parts of fennel were separated and identified by GC-MS.The relative content of each component was determined by the peak area normalization method.[Results]37,33,18,and 44 chemical components were separated from the volatile oil of fennel roots,stems,young leaves and fruit,respectively,accounting for 98.64%,99.34%,99.59% and 95.99% of the total volatile oil of corresponding parts.A total of 77 chemical components were identified in the four parts,of which 5 were common components.The main component of the volatile oil in the stems and young leaves was trans-anethole.The main components of the volatile oil in fruit were estragole and trans-anethole.And the main component of the volatile oil in the roots was dill apiol.The components in the volatile oil of fennel roots,stems,young leaves and fruit were different in type and content.[Conclusions]This study provides a theoretical reference for the further effective development and utilization of fennel resources.

GC-MS Analysis of Volatile Oils in Mango Leaves from Different Producing Areas

[Objectives]To analyze the composition of volatile oils from mango leaves from different producing areas.[Methods]The volatile oils in mango leaves from different areas was extracted by steam distillation,and the GC-MS method combined with retention index and peak area normalization method was used for qualitative and quantitative analysis.[Results]The volatile oils of mango leaves in southern provinces contained high content of terpenes,such asα-gurjunene(18.39%-34.13%),(+)-viridiflorene(8.95%-16.30%)and(E)-β-ocimene(3.49%-7.63%).The volatile oils components of mango leaves were significantly different in different provinces,for example,mango leaves produced in Hainan Province contained a large amount ofβ-selinene(23.00%),but not in other provinces.[Conclusions]The origin had a great influence on the composition and contents of volatile oils in mango leaves.

Analysis of Volatile Constituents in Lonicera japonica Thunb. from Different Origins by GC-MS

[Objective] This study aimed to analyze the volatile constituents in Lonicera japonica Thunb. from different origins. [Method] HP-5MS capillary columns were used and column temperature was controlled by a program. MS analysis was performed with EI and quadruple mass analyzer. The volatile constituents in L. japonica Thunb. were identified by NIST02 and Wiley275 libraries, and their relative contents were determined with chromatographic peak area normalization method. [Result] According to GC-MS total ion-current chromatograms, 35 volatile constituents were identified in L. japonica Thunb. from Guangxi Zhuang Autonomous Region, mainly including methyl linolenate, n-hexadecanoic acid and ζ-muurolene; 18 volatile constituents were identified in L. japonica Thunb. from Hunan Province, mainly including n-hexadecanoic acid, linoleic acid and α-curcumene. [Conclusion] Main volatile constituents in L. japonica Thunb. from two different origins varied significantly.

GC-MS Analysis of Chemical Constituents of Volatile Oil from Euonymus fortune Produced in Different Areas of Guangxi

The chemical constituents of the essential oil of Euonymus fortune from different areas were compared and analyzed by GC-MS.The results showed that 25 chromatographic peaks were separated and 13 chemical constituents were identified in Binyang County,Nanning City,accounting for 97.25%of the total volatile oil.Twenty nine chromatographic peaks were separated from Rong’an County,Liuzhou City,and 14 chemical constituents were identified,accounting for 89.91%of the total volatile oil.Thirty two chromatographic peaks were separated from Mengshan County,Wuzhou City,and 12 chemical constituents were identified,accounting for 92.16%of the total volatile oil.Twenty eight chromatographic peaks were separated from Rong County,Yulin City,and 11 chemical constituents were identified,accounting for 84.79%of the total volatile oil.Five of the chemical constituents of the volatile oil from the three places of origin are common to the four.They are phytol,palmitic acid,n-nonane,cumene,hexahydrofarnesyl acetone.It can be seen that the main components of volatile oil from different producing areas are different.

GC-MS Analysis and Antibacterial Activity Study of Volatile Oil from 11 Kinds of Aurantii Fructus Immaturus Processed Products

[Objectives]To study the volatile components and antibacterial effects of 11 kinds of Aurantii Fructus Immaturus processed products.[Methods]11 kinds of Aurantii Fructus Immaturus processed products were obtained according to the traditional processing method,the volatile oil was extracted by steam distillation,and the composition of volatile oil in the 11 kinds of processed products was analyzed by gas chromatography-mass spectrometry(GC-MS).the relative percentage content of each component in these 11 kinds of processed products was determined using the peak area normalization.The drug sensitivity activity of the volatile oil of these 11 kinds of processed products was tested using the K-B paper diffusion method and the minimum inhibition volume fraction of volatile oil of these 11 kinds of processed products was tested using the microdilution method.[Results]The highest yield of volatile oil of 11 kinds of these processed products was baking(5.193%),and the lowest was stir-bake to scorch(1.998%).A total of 36 chemical components were identified from the volatile oils of these 11 kinds of processed products.The components with the most volatile oil were stir-bake to scorch(24 kinds),and the components with the least volatile oil were the method of processing with rice-washed water(15 kinds).They contain 8 kinds of common chemical components,such as limonene,linalool,myrcene,α-pinene.The highest content of limonene came from processing with honey(60.93%),the lowest came from processing with rice-washed water(55.25%);the highest content of linalool came from processing with rice-washed water(7.139%),the lowest came from processing with honey(5.436%);the highest content of myrcene came from processing with honey(1.899%),the lowest came from stir-bake to scorch(1.632%);the highest content ofα-pinene came from raw Aurantii Fructus Immaturus(2.355%),and the lowest came from stir-bake to scorch(1.618%).The volatile oil of these 11 kinds of Aurantii Fructus Immaturus processed products has good antibacterial effect on Escherichia coli and Staphylococcus aureus.[Conclusions]The oil yields of volatile oils of 11 kinds of Aurantii Fructus Immaturus processed products are different,the content of limonene is significantly different,and the changes of other chemical components and their contents are not significantly different.The volatile oil of 11 kinds of Aurantii Fructus Immaturus processed products has certain antibacterial effect.

GC-MS Analysis of Chemical Constituents of Volatile Oils of the Yao Medicine Thunbergia grandiflora from Different Habitats in Guangxi

[Objectives]This study was conducted to compare and analyze the chemical constituents of the volatile oils of the Yao medicine Thunbergia grandiflora from different habitats.[Methods]A quartz capillary column DB-1MS,an EI ion source and a quadrupole mass analyzer were used for analysis.The chromatographic and mass spectrum information obtained was automatically retrieved and analyzed by data processing system and its memory spectrum library(Nist.08).The relative content of each chemical component in the volatile oil was determined by the peak area normalization method.[Results]Forty nine chromatographic peaks were isolated from the sample produced in Shitun,Bailongtan Town,Mashan County,and 24 chemical constituents were identified,accounting for 88.78% of the total volatile oil.Forty nine chromatographic peaks were isolated from the sample produced in Hongdu Village,Chengjiang Town,Duan County,and 30 chemical constituents were identified,accounting for 88.38% of the total volatile oil.Forty eight chromatographic peaks were isolated from the sample produced in Longwan Township,Du'an County,and 25 chemical constituents were identified,accounting for 80.01% of the total volatile oil.Nine chemical constituents were common to the volatile oils of the samples from the three habitats.[Conclusions]It could be seen that the main components of volatile oils from T.grandiflora produced in different areas are different.

上海白玉兰花香和鲜花精油挥发性成分的GC-MS分析研究

为了比较上海白玉兰花香和白玉兰鲜花精油的成分差异,采用顶空气质联用技术(HS-GC-MS)或气相色谱-质谱联用技术(GC-MS)进行挥发性成分的分析。结果表明,从白玉兰花香和白玉兰鲜花精油中共鉴定出44种成分,相同成分10种;白玉兰花香中共检测出26种成分,相对含量占其总量的96.29%,其主要成分为桉叶油醇(26.73%)、桧烯(23.29%)、β-蒎烯(18.77%)、β-月桂烯(12.22%)和α-蒎烯(6.83%);白玉兰鲜花精油中共检测出28种化学成分,相对含量占其总量的79.46%,主要成分包括苯乙醇(35.48%)、桉叶油醇(10.09%)、α-松油醇(9.12%)、柳杉二醇(5.69%)和4-松油醇(5.52%)。白玉兰花香和白玉兰鲜花精油成分在类别及相对含量上均有所差异,这为白玉兰资源的合理开发利用提供了理论依据。

Pharmacological effects of volatile oil from chrysanthemum and its associated mechanisms:a review

Volatile oil(VO)is the main chemical component of common plants in Chrysanthemum genus,and it possesses several beneficial pharmacological properties,including bacteriostatic,antioxidant,anti-tumor,anti-inflammatory,antipyretic,analgesic,antiosteoporotic,antihypertensive,sedative,and hypnotic effects.To date,research on the effective components of Chrysanthemum extract has mainly focused on flavonoids,whereas limited data are available on the chemical constituents and underlying mechanisms of action of the VO components.In this review,the pharmacological activities and mechanisms of VO are comprehensively reviewed with the aim of providing a foundation for further development for medicinal,aromatherapy,and diet therapy applications.

Study on the interaction between volatile oil components and skin lipids based on molecular docking techniques

Objective To analyze the interactions between different structural types of volatile oil compo-nents(VOCs)and skin lipid molecules;and investigate the mechanism of volatile oil in Chi-nese materia medica(VOCMM)as penetration enhancers.Methods In this study;210 different structural types of VOCs were selected from the VOCMM penetration enhancer database;and the molecular docking experiments were conducted with three main lipid molecules of skin:ceramide 2(CER2);cholesterol(CHL);and free fatty acid(FFA).Each VOC was docked individually with each lipid molecule.Cluster analysis was used to explore the relationship between the binding energy of VOCs and their molecular struc-tures.Nine specific pathogen-free(SPF)Sprague Dawley(SD)rats were randomly divided in-to Control;Nootkatone;and 3-Butylidenephthalide groups for in vitro percutaneous experi-ments;with three rats in each group.The donor pool solutions were 3%gastrodin;3%gas-trodin+3%nootkatone;and 3%gastrodin+3%3-butylidenephthalide;respectively.The pen-etration enhancing effects of VOCs with higher binding energy were evaluated by comparing the 12-hour cumulative percutaneous absorption of gastrodin(Q12;µg/cm²).Results(i)Most of the VOCs were non-hydrogen bonded to the hydrophobic parts of CHL and FFA;and hydrogen bonded to the head group of CER2.Among them;sesquiterpene ox-ides showed the most pronounced binding affinity to CER2.The VOCs with 2-4 rings(in-cluding carbon rings;benzene rings;and heterocycles)demonstrated stronger binding affini-ty for three skin lipid molecules compared with the VOCs without intramolecular rings(P<0.01).(ii)According to the cluster analysis;most of the VOCs that bond well to CER2 had 2-3 intramolecular rings.The non-oxygenated VOCs were bonded to CER2 in a hydrophobic manner.The oxygenated VOCs were mostly bonded to CER2 by hydrogen bonding.(iii)The results of Franz diffusion cell experiment showed that the Q12 of Control group was 260.60±25.09µg/cm2;and the transdermal absorption of gastrodin was significantly increased in Nootkatone group(Q12=5503.00±1080.00µg/cm²;P<0.01).The transdermal absorption of gastrodin was also increased in 3-Butylidenephthalide group(Q12=495.40±56.98µg/cm²;P>0.05).(iv)The type of oxygen-containing functional groups in VOCs was also an influencing factor of binding affinity to CER2.Conclusion The interactions between different types of VOCs with different structures in the VOCMM and three skin lipid molecules in the stratum corneum were investigated at the molecular level in this paper.This research provided theoretical guidance and data support for the screening of volatile oil-based penetration enhancers;and a simple and rapid method for studying the penetration-enhancing mechanism of volatile oils.

Effect of Centipeda Herba Volatile Oil on Allergic Rhinitis by Nasal Sniffing

[Objectives] To explore the efficacy of different concentrations of Centipeda Herba volatile oil in the treatment of allergic rhinitis (AR) by nasal sniffing and the optimal drug concentration.[Methods] Forty-eight mice were randomly divided into control group, blank group, alcohol treatment group, low concentration group, medium concentration group and high concentration group, with 8 mice in each group. Except for the blank group, each mouse in the other groups was intraperitoneally injected with 1 mL of a mixture of 50 μgOVA+5 mg [Al(OH)_(3)] +1 mL of normal saline for 14 d. The allergic rhinitis mouse model was successfully established by intranasal instillation of 5% OVA solution on both sides (20 μL per side, once a day) from the 15 th day after stimulation for 7 d. The blank group was treated with the same amount of saline as above. The volatile oil of Centipeda Herba was obtained by steam distillation and petroleum ether extraction, and then was made into 1.25%, 2.5% and 5% volatile oil of Centipeda Herba with 75% alcohol. The control group was stimulated once every other day with reagent 2 after 7 d of stimulation (maintenance) until the end of treatment. The blank group was treated with 5 mL saline by nasal sniffing for 30 d, twice in the morning and evening, 30 min each time. The alcohol treatment group was treated with 5 mL of 75% alcohol, and the low concentration group, the medium concentration group and the high concentration group were treated with 5 mL of 1.25%, 2.5% and 5% Centipeda Herba volatile oil, respectively. The treatment time was the same as that of the blank group, and the treatment process was carried out in their respective closed contamination boxes. Before and after the treatment, the frequency of sneezing, the frequency of scratching nose, the amount of nasal discharge, activity and other general characteristics of the mice were observed, and the allergic behavior score was carried out. Besides, the IgE content in the serum of the mice was determined, and the eosinophils in the nasal discharge were counted.[Results] The scores of mice before and after treatment showed that there was no significant difference in the alcohol treatment group before and after treatment ( P >0.05), and there was significant difference in the low, medium and high concentration groups before and after treatment ( P <0.05), except that there was no significant difference between the control group and the alcohol treatment group ( P >0.05), and there was significant difference among the other groups ( P <0.05). The levels of IgE and the number of eosinophils in peripheral serum of mice in the control group, alcohol treatment group, low concentration group, medium concentration group and high concentration group were higher than those in the blank group ( P <0.05), and there was no significant difference between the two groups ( P >0.05).[Conclusions] Volatile oil of Centipeda Herba can be used to treat allergic rhinitis by nasal sniffing, and 5% volatile oil of Centipeda Herba has the best effect. During the treatment, sneezing and runny nose in mice were reduced. The results showed that nasal sniffing was less irritating to the nasal cavity and not easy to produce discomfort, the utilization rate of drugs was higher than that of traditional therapy, and the volatile oil could be preserved longer than that of traditional Chinese medicine.

Exploration of the efficacy and mechanism of treating head wind disease with the combination change of ginger volatile oil and gingerol by using content-weighted network pharmacology technology

Background:Exploring the efficacy,potential components,and mechanism of the combination of ginger essential oil and gingerols in the treatment of head wind disease based on network pharmacology technology with content weight.Methods:The experimental groups were divided into:0:10,1:4,1:2,1:1,2:1,4:1,10:0.The relative content(Ri)of the chemical constituents of ginger's volatile oil was determined using gas chromatography-mass spectrometry(GC-MS).Additionally,the physicochemical and biological property parameters(LogP,MDCK,PPB,MW)of the components were considered.To assess the quantitative effect of the components,a grading score was performed,and the quantitative effect index(Ki)was calculated.Subsequently,the target effect index(Ti)was calculated by combining the component-target matching score(Fit score).Using these calculations,the target effect score A was determined under the influence of multiple components targeting different targets.Key targets with A≥1000 were identified.To predict the targets related to head wind disease,the Comparative Toxicogenomics Database(https://ctdbase.org/),Gene Cards(https://www.genecards.org/),and Disgenet database(https://www.disgenet.org/)were utilized.The key targets,obtained from different proportions of ginger's volatile oil and gingerol,were intersected with the predicted targets.This facilitated network pharmacological analysis and verification of the efficacy.Results:The content of volatile oil in ginger demonstrated an impact on key targets associated with the volatile oil group.Each specific combination of volatile oil consistently activated distinct pathways,with variations stemming from changes in content.Experimental testing revealed that different combinations of ginger's volatile oil and gingerol effectively alleviated migraine symptoms in rats.Conclusion:Through the application of content-weighted network pharmacology technology and pharmacodynamic verification,it was determined that altering the ratio between ginger's volatile oil and gingerol leads to variations in potential targets and pathways,consequently impacting its efficacy.

Identification of Volatile Compounds in Codfish(Gadus) by a Combination of Two Extraction Methods Coupled with GC-MS Analysis

Codfish is a kind of abyssal fish species with a great value in food industry. However, the flavor of codfish, especially the unpleasant odor, has caused serious problems in its processing. To accurately identify the volatile compounds in codfish, a combination of solid phase micro-extraction(SPME) method and simultaneous distillation extraction(SDE) method was used to extract the volatiles. Gas chromatography-mass spectrometry(GC-MS) along with Kovats indices(KI) and authentic standard compounds were used to identify the volatiles. The results showed that a total of 86 volatile compounds were identified in codfish, of them 24 were extracted by SDE, 69 compounds by SPME, and 10 compounds by both SDE and SPME. Seventy volatile compounds were found to have specific odors, of them 7 typical compounds contributed significantly to the flavor of codfish. Alcohols(i.e.,(E)-2-penten-1-ol and 2-octanol), esters(i.e., ethyl butyrate and methyl geranate), aldehydes(i.e., 2-dodecenal and pentadecanal) contributed the most to fresh flavor while nitrogen compounds, sulphur compounds, furans, as well as some ketones(i.e., 2-hydroxy-3-pentanone) brought unpleasant odor, such as fishy and earthy odor. It was indicated that the combination of multiple extraction methods and GC-MS analysis can enhance the accuracy of identification, and provide a reference for the further study on flavor of aquatic products.

Analysis of Volatile Compounds and Identification of Characteristic Aroma Components of <i>Toona sinensis</i>(A. Juss.) Roem. Using GC-MS and GC-O

In this study, volatile compounds present in Toona sinensis (A. Juss.) Roem (TS) were investigated and their characteristic aromatic components were identified using Headspace Solid-phase Microextraction (HS-SPME) followed by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Olfactometry (GC-O). The optimum conditions for extracting the volatiles from TS were achieved with the experimental parameters including the use of a 65 μm polydimethylsiloxane/divinyl benzene (PDMS/DVB) fibre, an extraction temperature of 40℃ and an extraction time of 30 min. Under these conditions, 56 volatile compounds were separated and 53 were identified by GC-MS. Among them, 21 sulfide compounds (42.146%) and 27 terpenes(55.984%) were found to be the major components. The sample was analyzed by GC-O and 26 elutes were sniffed and their sensory descriptions evaluated by an odor panelists. Analysis of the data indicated, two compounds cis and trans isomers of 2-Mercapto-3,4-dimethyl-2,3-dihydrothiophene were major contributors to the characteristic aroma of TS.

Low polar volatile constituents from Isodon excisa by GC-MS

An attempt had been made to isolate and identify the low polar volatile constituents from Isodon excisa. The dried plants were heat extracted with ethanol and then extracted with petroleum ether. The constituents were isolated and analyzed by GC-MS and then we retrieved them on NIST08 mass spectrometry database to determine their structures. Finally, successful results were achieved, 111 compounds were obtained and identified. 105 of them were reported for the first time in lsodon excisa and 22 of them were first found in natural products. Thus, the main constituents of it were found to be Sitosterol （33.28%）, 3-Amino-4-pyrazolecarbonitrile （9.55%）

GC-MS Analysis of Volatile Components in ‘Changping 8’ Apple Fruit

Solid-phase and micro-extraction combined with GC/MS(SPME-GC-MS) were employed to detect main aroma components in ‘Changping 8’ apple fruit. The results showed that, 29 kinds of compounds were identified from the aroma components in ‘Changping 8’ apple fruit, and they were aldehydes, alcohols, esters, ketones, etc. The main aroma components of apple fruits were acetic acid, hexyl ester, acetic acid, butyl ester,(E)-2-hexenal, 1-butanol, hexanal, and 1-hexanol.

HS-SPME-GC-MS Analysis on Volatile Components in Different Cultivars and Different Parts of Artemisia argyi

[Objectives] To analyze and compare the difference in volatile components of different cultivars and different parts of Artemisia argyi by using headspace solid phase microextraction( HS-SPME) and provide scientific basis for comprehensive development and rational utilization of Artemisia argyi. [Methods]Firstly,HS-SPME was used to extract the volatile components from the leaves,seeds,and stems of seven-tip Artemisia argyi,five-tip Artemisia argyi,and golden Artemisia argyi. Their chemical composition was analyzed by gas chromatographymass spectrometry( GC-MS),and the relative mass fraction of each component was determined by area normalization method. [Results] For the same variety of Artemisia argyi,the content of volatile components in leaves was higher than in seeds and stems except camphor,and the content of volatile components in seven-tip Artemisia argyi leaves was higher than in five-tip Artemisia argyi and golden Artemisia argyi leaves.HS-SPME-GC-MS analysis showed that eucalyptol,camphor,thujone,α-pinene,1-caryophyllene and caryophyllene oxide with higher content were detected in different parts of the different cultivars of Artemisia argyi. [Conclusions]Seven-tip Artemisia argyi leaves were the best medicinal parts of the three cultivars,followed by seven-tip Artemisia argyi seeds.

Detection of Volatile Components in Leaves and Bark of Picrasma quassioides by GC-MS

[Objectives]This study aimed to analyze the volatile oil components of the leaves and bark of Picrasma quassioides.[Methods]The volatile oils in the leaves and bark of P.quassioides were extracted using steam distillation(SD)method and identified with gas chromatography-mass spectrometry(GC-MS).Based on the mass spectrum database(NiST08),combined with artificial spectrum analysis,referring to relevant literature,the base peak and relative abundance were compared to identify the volatile components of the leaves and bark.The relative content of each component was determined by peak area normalization method.[Results]A total of 24 and 23 chemical components were identified in the leaves and bark of P.quassioides,respectively,including 11 common components.[Conclusions]Leaf and bark are different tissues.They contain different volatile chemical components,of which the contents vary greatly.They should be treated differently in the further research and development of P.quassioides resources.

Analysis of Volatile Chemical Constituents in Callistemon rigidus Flowers by GC-MS

Volatile components of Callistemon rigidus were analyzed by headspace solid phase microextraction-gas chromatography/mass spectrometry.The results showed that 34 volatile components,including alkenes,esters,alcohols,and a small amount of acids,heterocycles and alkanes,were identified from C.rigidus flowers.The main components were eucalyptol,aspidinol,terpilenol,vanillylacetone,α-phellandrene,4-pyran-4-1,2,3-dihydro-3,5-dihydroxy-6-methyl,pinene,3-methylcatechol,naphthalene,(+)-limonene,7-methylxanthine and (+)-aromadendrene,the contents of which were 35.39%,10.09%,8.86%,4.51%,3.25%,2.81%,2.75%,2.43%,2.29%,1.3%,1.12% and 1.07%,respectively,all of which accounted for 75.69% of the total relative content.Monoterpenoids are the main components of various types of essential oils.This study provides a theoretical basis for the deep processing of this variety.