Separation of shelled walnut particles was studied on two varieties of Persian walnut,Poost-Kaghazi and Poost-Sangi using pneumatic method.The moisture contents of the samples were determined.The particles were consid...Separation of shelled walnut particles was studied on two varieties of Persian walnut,Poost-Kaghazi and Poost-Sangi using pneumatic method.The moisture contents of the samples were determined.The particles were considered in three categories of shell,kernel and shell-kernel together.Each category was manually classified based on their size,in three portions of 1/8,1/4,and 1/2,as well as the whole kernel and whole walnut.The terminal velocity of each group was determined.The shelled walnuts were sieved and classified in three groups of small,medium and large.The effects of separation time(5,10 and 15 seconds),feeding value(50 to 80 gr)and air velocity on separation of the kernels and shells were studied for both varieties.The interaction effects were also studied for three walnut sizes(small,medium and large).The terminal velocity was the highest for the whole walnut and the whole kernel while it was lowest for 1/4 and 1/8 of the shell.The best separation was performed at air velocities of 9.20,10.04 and 10.94 m/s with 98.2%,98.9%and 98.2%,respectively.展开更多
通过吸附、解吸试验,筛选适合分离纯化核桃青皮多酚的大孔树脂,并对分离纯化条件进行优化。以没食子酸、原儿茶素等9种标准品绘制标准曲线,采用HPLC对所得核桃青皮多酚物质进行定性和定量分析。结果表明:NKA-9树脂是分离纯化核桃青皮多...通过吸附、解吸试验,筛选适合分离纯化核桃青皮多酚的大孔树脂,并对分离纯化条件进行优化。以没食子酸、原儿茶素等9种标准品绘制标准曲线,采用HPLC对所得核桃青皮多酚物质进行定性和定量分析。结果表明:NKA-9树脂是分离纯化核桃青皮多酚的最佳树脂,吸附条件为上样液多酚浓度0.574 mg/m L,p H 1.0,上样速率1.3 m L/min,吸附率可达90.1%;洗脱条件为体积分数50%的乙醇洗脱剂,p H 3.0,洗脱速率0.43 m L/min,解吸率达87.9%。经HPLC鉴定和分析,核桃青皮酚类物质主要由原儿茶素、绿原酸、咖啡酸、表儿茶素、阿魏酸、芦丁等单体组成,其中含量较高的为表儿茶素、原儿茶素和芦丁。展开更多
比较6种不同极性大孔树脂对核桃青皮提取物中胡桃醌的静态吸附和解吸性能,筛选出最佳的大孔树脂。通过与胡桃醌标准品HPLC保留时间相比较的方法对胡桃醌含量进行分析。结果表明:D101树脂为纯化核桃青皮胡桃醌的最佳树脂,较佳的动态吸附...比较6种不同极性大孔树脂对核桃青皮提取物中胡桃醌的静态吸附和解吸性能,筛选出最佳的大孔树脂。通过与胡桃醌标准品HPLC保留时间相比较的方法对胡桃醌含量进行分析。结果表明:D101树脂为纯化核桃青皮胡桃醌的最佳树脂,较佳的动态吸附条件为上样液胡桃醌浓度0.114 mg/m L,p H 2.0,上样速率为1.0 m L/min,吸附率可达81.85%;较佳的洗脱条件为体积分数95%的丙酮,p H 4.0,洗脱速率为0.33 m L/min,解吸率为76.5%。经HPLC鉴定和分析,D101分离纯化核桃青皮胡桃醌不会造成组成变化和较大损失。D101大孔树脂对核桃青皮胡桃醌有较好的分离纯化效果。展开更多
文摘Separation of shelled walnut particles was studied on two varieties of Persian walnut,Poost-Kaghazi and Poost-Sangi using pneumatic method.The moisture contents of the samples were determined.The particles were considered in three categories of shell,kernel and shell-kernel together.Each category was manually classified based on their size,in three portions of 1/8,1/4,and 1/2,as well as the whole kernel and whole walnut.The terminal velocity of each group was determined.The shelled walnuts were sieved and classified in three groups of small,medium and large.The effects of separation time(5,10 and 15 seconds),feeding value(50 to 80 gr)and air velocity on separation of the kernels and shells were studied for both varieties.The interaction effects were also studied for three walnut sizes(small,medium and large).The terminal velocity was the highest for the whole walnut and the whole kernel while it was lowest for 1/4 and 1/8 of the shell.The best separation was performed at air velocities of 9.20,10.04 and 10.94 m/s with 98.2%,98.9%and 98.2%,respectively.
文摘为提高核桃的附加值,以核桃粕为原料,将核桃谷蛋白多肽与锌进行螯合,利用扫描电子显微镜、傅里叶变换红外光谱、X射线衍射光谱等分析手段表征多肽与锌离子的螯合特征,并通过超滤、凝胶柱层析、液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)技术筛选出与锌结合能力较高的肽段,结合分子对接技术探究二者的具体结合位点和结合能力。结果表明,核桃谷蛋白多肽与锌离子发生了螯合反应,螯合前后多肽的表面微观结构和结晶程度均有显著差异;经超滤、凝胶柱层析分离后,F41组分螯合能力最强,为(117.43±1.99)mg/g;LC-MS/MS结合虚拟筛选得到5条无毒无潜在致敏性且具有潜在生物活性的肽段;利用分子对接技术模拟分析肽锌螯合物的结合过程,发现五肽Phe-Asp-Ala-Asp-Phe(FDADF)可与锌离子形成结构稳定的复合物,通过配位键、氢键和疏水相互作用结合,结合能最低,为-7.27 kcal/mol,结合位点主要为天冬氨酸侧链的羧基氧原子(Asp4:O-Zn)。本研究可为肽锌螯合物的开发应用提供理论依据。
文摘通过吸附、解吸试验,筛选适合分离纯化核桃青皮多酚的大孔树脂,并对分离纯化条件进行优化。以没食子酸、原儿茶素等9种标准品绘制标准曲线,采用HPLC对所得核桃青皮多酚物质进行定性和定量分析。结果表明:NKA-9树脂是分离纯化核桃青皮多酚的最佳树脂,吸附条件为上样液多酚浓度0.574 mg/m L,p H 1.0,上样速率1.3 m L/min,吸附率可达90.1%;洗脱条件为体积分数50%的乙醇洗脱剂,p H 3.0,洗脱速率0.43 m L/min,解吸率达87.9%。经HPLC鉴定和分析,核桃青皮酚类物质主要由原儿茶素、绿原酸、咖啡酸、表儿茶素、阿魏酸、芦丁等单体组成,其中含量较高的为表儿茶素、原儿茶素和芦丁。
文摘比较6种不同极性大孔树脂对核桃青皮提取物中胡桃醌的静态吸附和解吸性能,筛选出最佳的大孔树脂。通过与胡桃醌标准品HPLC保留时间相比较的方法对胡桃醌含量进行分析。结果表明:D101树脂为纯化核桃青皮胡桃醌的最佳树脂,较佳的动态吸附条件为上样液胡桃醌浓度0.114 mg/m L,p H 2.0,上样速率为1.0 m L/min,吸附率可达81.85%;较佳的洗脱条件为体积分数95%的丙酮,p H 4.0,洗脱速率为0.33 m L/min,解吸率为76.5%。经HPLC鉴定和分析,D101分离纯化核桃青皮胡桃醌不会造成组成变化和较大损失。D101大孔树脂对核桃青皮胡桃醌有较好的分离纯化效果。
