Underwater exploration has been an attractive topic for understanding the very nature of the lakes and even deep oceans.In recent years,extensive efforts have been devoted to developing functional materials and their ...Underwater exploration has been an attractive topic for understanding the very nature of the lakes and even deep oceans.In recent years,extensive efforts have been devoted to developing functional materials and their integrated devices for underwater information capturing.However,there still remains a great challenge for water depth detection and vibration monitoring in a high-efficient,controllable,and scalable way.Inspired by the lateral line of fish that can sensitively sense the water depth and environmental stimuli,an ultrathin,elastic,and adaptive underwater sensor based on Ecoflex matrix with embedded assembled graphene sheets is fabricated.The graphene structured thin film is endowed with favourable adaptive and morphable features,which can conformally adhere to the structural surface and transform to a bulged state driven by water pressure.Owing to the introduction of the graphene-based layer,the integrated sensing system can actively detect the water depth with a wide range of 0.3-1.8 m.Furthermore,similar to the fish,the mechanical stimuli from land(e.g.knocking,stomping)and water(e.g.wind blowing,raining,fishing)can also be sensitively captured in real time.This graphene structured thin-film system is expected to demonstrate significant potentials in underwater monitoring,communication,and risk avoidance.展开更多
To address the challenges of missed detections in water surface target detection using solely visual algorithms in unmanned surface vehicle(USV)perception,this paper proposes a method based on the fusion of visual and...To address the challenges of missed detections in water surface target detection using solely visual algorithms in unmanned surface vehicle(USV)perception,this paper proposes a method based on the fusion of visual and LiDAR point-cloud projection for water surface target detection.Firstly,the visual recognition component employs an improved YOLOv7 algorithmbased on a self-built dataset for the detection of water surface targets.This algorithm modifies the original YOLOv7 architecture to a Slim-Neck structure,addressing the problemof excessive redundant information during feature extraction in the original YOLOv7 network model.Simultaneously,this modification simplifies the computational burden of the detector,reduces inference time,and maintains accuracy.Secondly,to tackle the issue of sample imbalance in the self-built dataset,slide loss function is introduced.Finally,this paper replaces the original Complete Intersection over Union(CIoU)loss function with the Minimum Point Distance Intersection over Union(MPDIoU)loss function in the YOLOv7 algorithm,which accelerates model learning and enhances robustness.To mitigate the problem of missed recognitions caused by complex water surface conditions in purely visual algorithms,this paper further adopts the fusion of LiDAR and camera data,projecting the threedimensional point-cloud data from LiDAR onto a two-dimensional pixel plane.This significantly reduces the rate of missed detections for water surface targets.展开更多
A novel luminescent coordination compound Eu(TTA)3(DEDAF)(1, TTA = 1,1,1-trifluoro-3-(2-thenoyl)acetone, DEDAF = 9,9-diethyl-4,5-diazafluoren) has been synthesized and fully characterized by infrared spectrum,...A novel luminescent coordination compound Eu(TTA)3(DEDAF)(1, TTA = 1,1,1-trifluoro-3-(2-thenoyl)acetone, DEDAF = 9,9-diethyl-4,5-diazafluoren) has been synthesized and fully characterized by infrared spectrum, elemental analysis, UV-vis spectrum, etc. X-ray single-crystal diffraction analysis reveals that compound 1 shows a mononuclear structure with the europium atom in coordinating to one DAF and three TTA ligands. The mononuclear structure units are assembled into a 3-D polymer by hydrogen bonds and π-π interactions. Photoluminescent property of 1 was investigated in detail at room temperature. Complex 1 emits strong red luminescence. However, it could be quenched even by small amount of water. The fluorescence intensity at 614 nm decreases linearly with the water content increasing(vol% in acetonitrile) in the range of 0.025~0.2% under 278 nm excitation. Thermogravimetric analysis has also been studied, which demonstrates good thermal stability of 1.展开更多
Understanding the dynamics of surface water area and their drivers is crucial for human survival and ecosystem stability in inland arid and semi-arid areas.This study took Gansu Province,China,a typical area with comp...Understanding the dynamics of surface water area and their drivers is crucial for human survival and ecosystem stability in inland arid and semi-arid areas.This study took Gansu Province,China,a typical area with complex terrain and variable climate,as the research subject.Based on Google Earth Engine,we used Landsat data and the Open-surface Water Detection Method with Enhanced Impurity Control method to monitor the spatiotemporal dynamics of surface water area in Gansu Province from 1985 to 2022,and quantitatively analyzed the main causes of regional differences in surface water area.The findings revealed that surface water area in Gansu Province expanded by 406.88 km2 from 1985 to 2022.Seasonal surface water area exhibited significant fluctuations,while permanent surface water area showed a steady increase.Notably,terrestrial water storage exhibited a trend of first decreasing and then increasing,correlated with the dynamics of surface water area.Climate change and human activities jointly affected surface hydrological processes,with the impact of climate change being slightly higher than that of human activities.Spatially,climate change affected the'source'of surface water to a greater extent,while human activities tended to affect the'destination'of surface water.Challenges of surface water resources faced by inland arid and semi-arid areas like Gansu Province are multifaceted.Therefore,we summarized the surface hydrology patterns typical in inland arid and semi-arid areas and tailored surface water'supply-demand'balance strategies.The study not only sheds light on the dynamics of surface water area in Gansu Province,but also offers valuable insights for ecological protection and surface water resource management in inland arid and semi-arid areas facing water scarcity.展开更多
In a karst tunnel, fissures or cracks that are filled with weathered materials are a type of potential water outlet as they are easily triggered and converted into groundwater outlets under the influence of high groun...In a karst tunnel, fissures or cracks that are filled with weathered materials are a type of potential water outlet as they are easily triggered and converted into groundwater outlets under the influence of high groundwater pressure. A terrible water inrush caused by potential water outlets can seriously hinder the project construction. Potential water outlets and water sources that surrounding the tunnel must be detected before water inflow can be treated. This paper provides a successful case of the detection and treatment of water inflow in a karst tunnel and proposes a potential water outlet detection(PWOD) method in which heavy rainfall(>50 mm/d) is considered a trigger for a potential water outlet. The Daba tunnel located in Hunan province, China, has been constructed in a karst stratum where the rock mass has been weathered intensely by the influence of two faults. Heavy rain triggered some potential water outlets, causing a serious water inrush. The PWOD method was applied in this project for the treatment of water inflow, and six potential water outlets in total were identified through three heavy rains. Meanwhile, a geophysical prospecting technique was also used to detect water sources. The connections between water outlets and water sources were identified with a 3-D graphic that included all of them. According to the distribution of water outlets and water sources, the detection area was divided into three sections and separately treated by curtain grouting.展开更多
The leakage control is an important task, because it is associated with some problems such as economic loss, safety concerns, and environmental damages. The pervious methods which have already been devised for leakage...The leakage control is an important task, because it is associated with some problems such as economic loss, safety concerns, and environmental damages. The pervious methods which have already been devised for leakage detection are not only expensive and time consuming, but also have a low efficient. As a result, the global leakage detection methods such as leak detection based on simulation and calibration of the network have been considered recently. In this research, leak detection based on calibration in two hypothetical and a laboratorial networks is considered. Additionally a novel optimization method called step-by-step elimination method (SSEM) combining with a genetic algorithm (GA) is introduced to calibration and leakage detection in networks. This method step-by-step detects and eliminates the nodes that provide no contribution in leakage among uncertain parameters of calibration of a network. The proposed method initiates with an ordinary calibration for a studied network, follow by elimination of suspicious nodes among adjusted parameters, then, the network is re-calibrated. Finally the process is repeated until the numbers of unknown demands are equal to the desired numbers or the exact leakage locations and values are determined. These investigations illustrate the capability of this method for detecting the locations and sizes of leakages.展开更多
Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not mee...Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.展开更多
The breaking of wind-generated waves is an important phenomenon in the ocean, having close relation to many aspects of the ocean, such as air-sea interaction, ocean wave dynamics, oceanic remote sensing and ocean engi...The breaking of wind-generated waves is an important phenomenon in the ocean, having close relation to many aspects of the ocean, such as air-sea interaction, ocean wave dynamics, oceanic remote sensing and ocean engineering. The first problem encountered in both its theoretical study and practical measurement is how to detect the breaking of waves.展开更多
Objective:To analyse molecular detection of coliforms and shorten the time of PCR.Methods:Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in ...Objective:To analyse molecular detection of coliforms and shorten the time of PCR.Methods:Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably number(MPN)method for 16 artificial and 101 field samples.The molecular method was also conducted on isolated coliforms from positive MPN samples;standard sample for verification of microbial method certificated reference material;isolated strains from certificated reference material and standard bacteria.The PCR and electrophoresis parameters were changed for reducing the operation time.Results:Results of PCR for lacZ and uidA genes were similar in all of standard,operational and artificial samples and showed the 876 bp and 147 bp bands of lacZ and uidA genes by multiplex PCR.PCR results were confirmed by MPN culture method by sensitivity 86%(95%CI:0.71-0.93).Also the total execution time,with a successful change of factors,was reduced to less than two and a half hour.Conclusions:Multiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak city.It's recommended to be used at least as an initial screening test,and then the positive samples could be randomly tested by MPN.展开更多
<i>Entamoeba histolytica</i> is an anaerobic parasitic protozoan and well known as a human pathogen, while its close relative, <i>Entamoeba dispar</i>, also possesses similar characteristics as...<i>Entamoeba histolytica</i> is an anaerobic parasitic protozoan and well known as a human pathogen, while its close relative, <i>Entamoeba dispar</i>, also possesses similar characteristics as an infectious agent. These microorganisms are generally transmitted in fecal-contaminated water. However, <i>E. dispar</i> present in industrial wastewater is also capable of creating biofilms that can cause adverse impacts in piping networks. Therefore, it is important to detect both of these protozoan species in water and to find a cost-effective technique for inactivation or management control. This review article summarizes the available detection methods in water and wastewater matrices along with feasible disinfection techniques.展开更多
A simple, economical, and sensitive capillary electrophoresis (CE) method integrated with capacitively coupled contactless conductivity detection was developed for the determination of metal ions such as K<sup>+...A simple, economical, and sensitive capillary electrophoresis (CE) method integrated with capacitively coupled contactless conductivity detection was developed for the determination of metal ions such as K<sup>+</sup>, Na<sup>+</sup>, Mg<sup>2+</sup>, Sr<sup>2+</sup>, Ca<sup>2+</sup> in drinking water. 18-Crown-6 ether and Hexadecyltrimethylammonium Bromide (CTAB) were employed as complexing reagents. The effects of electrolyte additives, citric acid buffer solution, and other separation conditions of CE were comprehensively investigated and carefully optimized. The best results were obtained in a running buffer solution composed of citric acid (12 mM), 18-crown-6 ether (0.2 mM), and CTAB (0.015 mM) at pH 3.5. Under these conditions, a complete separation of five metal ions was successfully achieved in less than 12 min. The limits of detection for the optimal procedure were determined to be in the range of 0.02 - 0.2 mg·L<sup>-1</sup>. The repeatability with respect to migration times and peak areas, expressed as relative standard deviations, was better than 2.3% and 5.1%, respectively. Evaluation of the efficiency of the methodology indicated that it was reliable for the determination of metal ions in six different brands of drinking water samples.展开更多
Taking 91105 working face as the research object, the observation method of water flowing fracture<span style="font-family:Verdana;">d</span><span style="font-family:Verdana;"> zo...Taking 91105 working face as the research object, the observation method of water flowing fracture<span style="font-family:Verdana;">d</span><span style="font-family:Verdana;"> zone and the layout of mining holes were determined by analyzing the field geological structure</span><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">It was shown that the fractured zone height and the ratio given by the measured method were 52.33 and 12.46, respectively. By the numerical simulation method with the software of UDEC, the fractured zone height and the ratio were 42.5 and 10.12. By comparison of measured height data and UDEC numerical simulation, there were some differences between the measured height and the calculated results of UDEC numerical simulation method. The method of simulation can be used as the technical basis for the design of waterproof coal pillar in the future.</span>展开更多
Algae blooms pose a threat to water quality by depleting oxygen during decomposition and also cause other issues with water quality and water use. Algae biomass is traditional monitored through field samples analyzed ...Algae blooms pose a threat to water quality by depleting oxygen during decomposition and also cause other issues with water quality and water use. Algae biomass is traditional monitored through field samples analyzed for chlorophyll-a, a pigment present in all algae. Field sampling can be time- and cost-intensive, especially in areas that are difficult to access and provides only limited spatial coverage. Estimations of algal biomass based on remote sensing data have been explored over the past two decades as a supplement to information obtained from limited field samples. We use Landsat data to develop and demonstrate seasonal remote sensing models, a relatively recent method, to evaluate spatial and temporal algae distributions for the Jordanelle Reservoir, located in north-central Utah. Remote sensing of chlorophyll as a monitoring and analysis method can provide a more spatially complete representation of algae distribution and biomass;information that is difficult to obtain using point samples.展开更多
New methods of analysis for water quality monitoring to detect inorganic substances are required to meet the demands of determining concentration, particularly at low detection limits, analysing speciation and even id...New methods of analysis for water quality monitoring to detect inorganic substances are required to meet the demands of determining concentration, particularly at low detection limits, analysing speciation and even identifying the pollution source. Such information is essential to inform public health decisions and to comply with more stringent legislation. This paper concentrates on two case studies, reviewing the development in monitoring methods, and predicting future trends. Arsenic and nitrates detection was selected as these pollutants are particularly problematic from a human health perspective. Additionally, the challenges faced in developing monitoring methods for these chemicals are relevant to a wide range of other inorganics. The current state of the art in detection approaches for these chemicals are discussed along with recommendations for future research to further improve the methods.展开更多
基金supported by the Natural Science Foundation of China(51803226,52073295)the Sino-German Mobility Program(M-0424)+3 种基金Key Research Program of Frontier Sciences,Chinese Academy of Sciences(QYZDB-SSWSLH036)Bureau of International Cooperation,Chinese Academy of Sciences(174433KYSB20170061)Ningbo Science and Technology Bureau(2021Z127)K.C.Wong Education Foundation(GJTD-2019-13).
文摘Underwater exploration has been an attractive topic for understanding the very nature of the lakes and even deep oceans.In recent years,extensive efforts have been devoted to developing functional materials and their integrated devices for underwater information capturing.However,there still remains a great challenge for water depth detection and vibration monitoring in a high-efficient,controllable,and scalable way.Inspired by the lateral line of fish that can sensitively sense the water depth and environmental stimuli,an ultrathin,elastic,and adaptive underwater sensor based on Ecoflex matrix with embedded assembled graphene sheets is fabricated.The graphene structured thin film is endowed with favourable adaptive and morphable features,which can conformally adhere to the structural surface and transform to a bulged state driven by water pressure.Owing to the introduction of the graphene-based layer,the integrated sensing system can actively detect the water depth with a wide range of 0.3-1.8 m.Furthermore,similar to the fish,the mechanical stimuli from land(e.g.knocking,stomping)and water(e.g.wind blowing,raining,fishing)can also be sensitively captured in real time.This graphene structured thin-film system is expected to demonstrate significant potentials in underwater monitoring,communication,and risk avoidance.
基金supported by the National Natural Science Foundation of China(No.51876114)the Shanghai Engineering Research Center of Marine Renewable Energy(Grant No.19DZ2254800).
文摘To address the challenges of missed detections in water surface target detection using solely visual algorithms in unmanned surface vehicle(USV)perception,this paper proposes a method based on the fusion of visual and LiDAR point-cloud projection for water surface target detection.Firstly,the visual recognition component employs an improved YOLOv7 algorithmbased on a self-built dataset for the detection of water surface targets.This algorithm modifies the original YOLOv7 architecture to a Slim-Neck structure,addressing the problemof excessive redundant information during feature extraction in the original YOLOv7 network model.Simultaneously,this modification simplifies the computational burden of the detector,reduces inference time,and maintains accuracy.Secondly,to tackle the issue of sample imbalance in the self-built dataset,slide loss function is introduced.Finally,this paper replaces the original Complete Intersection over Union(CIoU)loss function with the Minimum Point Distance Intersection over Union(MPDIoU)loss function in the YOLOv7 algorithm,which accelerates model learning and enhances robustness.To mitigate the problem of missed recognitions caused by complex water surface conditions in purely visual algorithms,this paper further adopts the fusion of LiDAR and camera data,projecting the threedimensional point-cloud data from LiDAR onto a two-dimensional pixel plane.This significantly reduces the rate of missed detections for water surface targets.
基金supported by the Natural Science Foundation of Zhejiang Province(No.LY16B030009)National Natural Science Foundation of China(No.61205184)521 Talent Cultivation of Zhejiang Sci-Tech University(521 talent project of ZSTU)
文摘A novel luminescent coordination compound Eu(TTA)3(DEDAF)(1, TTA = 1,1,1-trifluoro-3-(2-thenoyl)acetone, DEDAF = 9,9-diethyl-4,5-diazafluoren) has been synthesized and fully characterized by infrared spectrum, elemental analysis, UV-vis spectrum, etc. X-ray single-crystal diffraction analysis reveals that compound 1 shows a mononuclear structure with the europium atom in coordinating to one DAF and three TTA ligands. The mononuclear structure units are assembled into a 3-D polymer by hydrogen bonds and π-π interactions. Photoluminescent property of 1 was investigated in detail at room temperature. Complex 1 emits strong red luminescence. However, it could be quenched even by small amount of water. The fluorescence intensity at 614 nm decreases linearly with the water content increasing(vol% in acetonitrile) in the range of 0.025~0.2% under 278 nm excitation. Thermogravimetric analysis has also been studied, which demonstrates good thermal stability of 1.
基金This research was supported by the Third Xinjiang Scientific Expedition Program(2021xjkk010102)the National Natural Science Foundation of China(41261047,41761043)+1 种基金the Science and Technology Plan of Gansu Province,China(20YF3FA042)the Youth Teacher Scientific Capability Promoting Project of Northwest Normal University,Gansu Province,China(NWNU-LKQN-17-7).
文摘Understanding the dynamics of surface water area and their drivers is crucial for human survival and ecosystem stability in inland arid and semi-arid areas.This study took Gansu Province,China,a typical area with complex terrain and variable climate,as the research subject.Based on Google Earth Engine,we used Landsat data and the Open-surface Water Detection Method with Enhanced Impurity Control method to monitor the spatiotemporal dynamics of surface water area in Gansu Province from 1985 to 2022,and quantitatively analyzed the main causes of regional differences in surface water area.The findings revealed that surface water area in Gansu Province expanded by 406.88 km2 from 1985 to 2022.Seasonal surface water area exhibited significant fluctuations,while permanent surface water area showed a steady increase.Notably,terrestrial water storage exhibited a trend of first decreasing and then increasing,correlated with the dynamics of surface water area.Climate change and human activities jointly affected surface hydrological processes,with the impact of climate change being slightly higher than that of human activities.Spatially,climate change affected the'source'of surface water to a greater extent,while human activities tended to affect the'destination'of surface water.Challenges of surface water resources faced by inland arid and semi-arid areas like Gansu Province are multifaceted.Therefore,we summarized the surface hydrology patterns typical in inland arid and semi-arid areas and tailored surface water'supply-demand'balance strategies.The study not only sheds light on the dynamics of surface water area in Gansu Province,but also offers valuable insights for ecological protection and surface water resource management in inland arid and semi-arid areas facing water scarcity.
基金supported by the National Key Research and Development Project (Grant No.2016YFC0801604)Natural Science Foundation of Shandong Province (Grant No.ZR2017MEE070)
文摘In a karst tunnel, fissures or cracks that are filled with weathered materials are a type of potential water outlet as they are easily triggered and converted into groundwater outlets under the influence of high groundwater pressure. A terrible water inrush caused by potential water outlets can seriously hinder the project construction. Potential water outlets and water sources that surrounding the tunnel must be detected before water inflow can be treated. This paper provides a successful case of the detection and treatment of water inflow in a karst tunnel and proposes a potential water outlet detection(PWOD) method in which heavy rainfall(>50 mm/d) is considered a trigger for a potential water outlet. The Daba tunnel located in Hunan province, China, has been constructed in a karst stratum where the rock mass has been weathered intensely by the influence of two faults. Heavy rain triggered some potential water outlets, causing a serious water inrush. The PWOD method was applied in this project for the treatment of water inflow, and six potential water outlets in total were identified through three heavy rains. Meanwhile, a geophysical prospecting technique was also used to detect water sources. The connections between water outlets and water sources were identified with a 3-D graphic that included all of them. According to the distribution of water outlets and water sources, the detection area was divided into three sections and separately treated by curtain grouting.
文摘The leakage control is an important task, because it is associated with some problems such as economic loss, safety concerns, and environmental damages. The pervious methods which have already been devised for leakage detection are not only expensive and time consuming, but also have a low efficient. As a result, the global leakage detection methods such as leak detection based on simulation and calibration of the network have been considered recently. In this research, leak detection based on calibration in two hypothetical and a laboratorial networks is considered. Additionally a novel optimization method called step-by-step elimination method (SSEM) combining with a genetic algorithm (GA) is introduced to calibration and leakage detection in networks. This method step-by-step detects and eliminates the nodes that provide no contribution in leakage among uncertain parameters of calibration of a network. The proposed method initiates with an ordinary calibration for a studied network, follow by elimination of suspicious nodes among adjusted parameters, then, the network is re-calibrated. Finally the process is repeated until the numbers of unknown demands are equal to the desired numbers or the exact leakage locations and values are determined. These investigations illustrate the capability of this method for detecting the locations and sizes of leakages.
基金Supported by the Basic Scientific Fund for National Public Research Institutes of China(Nos.GY02-2011T10,2015P07)the Qingdao Talent Program(No.13-CX-20)+1 种基金the National Natural Science Foundation of China(Nos.31100567,41176061)the National Natural Science Foundation for Creative Groups(No.41521064)
文摘Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.
文摘The breaking of wind-generated waves is an important phenomenon in the ocean, having close relation to many aspects of the ocean, such as air-sea interaction, ocean wave dynamics, oceanic remote sensing and ocean engineering. The first problem encountered in both its theoretical study and practical measurement is how to detect the breaking of waves.
基金Supported by the Ministry of Power of I.R.Iran(Grant No.201)
文摘Objective:To analyse molecular detection of coliforms and shorten the time of PCR.Methods:Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably number(MPN)method for 16 artificial and 101 field samples.The molecular method was also conducted on isolated coliforms from positive MPN samples;standard sample for verification of microbial method certificated reference material;isolated strains from certificated reference material and standard bacteria.The PCR and electrophoresis parameters were changed for reducing the operation time.Results:Results of PCR for lacZ and uidA genes were similar in all of standard,operational and artificial samples and showed the 876 bp and 147 bp bands of lacZ and uidA genes by multiplex PCR.PCR results were confirmed by MPN culture method by sensitivity 86%(95%CI:0.71-0.93).Also the total execution time,with a successful change of factors,was reduced to less than two and a half hour.Conclusions:Multiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak city.It's recommended to be used at least as an initial screening test,and then the positive samples could be randomly tested by MPN.
文摘<i>Entamoeba histolytica</i> is an anaerobic parasitic protozoan and well known as a human pathogen, while its close relative, <i>Entamoeba dispar</i>, also possesses similar characteristics as an infectious agent. These microorganisms are generally transmitted in fecal-contaminated water. However, <i>E. dispar</i> present in industrial wastewater is also capable of creating biofilms that can cause adverse impacts in piping networks. Therefore, it is important to detect both of these protozoan species in water and to find a cost-effective technique for inactivation or management control. This review article summarizes the available detection methods in water and wastewater matrices along with feasible disinfection techniques.
文摘A simple, economical, and sensitive capillary electrophoresis (CE) method integrated with capacitively coupled contactless conductivity detection was developed for the determination of metal ions such as K<sup>+</sup>, Na<sup>+</sup>, Mg<sup>2+</sup>, Sr<sup>2+</sup>, Ca<sup>2+</sup> in drinking water. 18-Crown-6 ether and Hexadecyltrimethylammonium Bromide (CTAB) were employed as complexing reagents. The effects of electrolyte additives, citric acid buffer solution, and other separation conditions of CE were comprehensively investigated and carefully optimized. The best results were obtained in a running buffer solution composed of citric acid (12 mM), 18-crown-6 ether (0.2 mM), and CTAB (0.015 mM) at pH 3.5. Under these conditions, a complete separation of five metal ions was successfully achieved in less than 12 min. The limits of detection for the optimal procedure were determined to be in the range of 0.02 - 0.2 mg·L<sup>-1</sup>. The repeatability with respect to migration times and peak areas, expressed as relative standard deviations, was better than 2.3% and 5.1%, respectively. Evaluation of the efficiency of the methodology indicated that it was reliable for the determination of metal ions in six different brands of drinking water samples.
文摘Taking 91105 working face as the research object, the observation method of water flowing fracture<span style="font-family:Verdana;">d</span><span style="font-family:Verdana;"> zone and the layout of mining holes were determined by analyzing the field geological structure</span><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">It was shown that the fractured zone height and the ratio given by the measured method were 52.33 and 12.46, respectively. By the numerical simulation method with the software of UDEC, the fractured zone height and the ratio were 42.5 and 10.12. By comparison of measured height data and UDEC numerical simulation, there were some differences between the measured height and the calculated results of UDEC numerical simulation method. The method of simulation can be used as the technical basis for the design of waterproof coal pillar in the future.</span>
文摘Algae blooms pose a threat to water quality by depleting oxygen during decomposition and also cause other issues with water quality and water use. Algae biomass is traditional monitored through field samples analyzed for chlorophyll-a, a pigment present in all algae. Field sampling can be time- and cost-intensive, especially in areas that are difficult to access and provides only limited spatial coverage. Estimations of algal biomass based on remote sensing data have been explored over the past two decades as a supplement to information obtained from limited field samples. We use Landsat data to develop and demonstrate seasonal remote sensing models, a relatively recent method, to evaluate spatial and temporal algae distributions for the Jordanelle Reservoir, located in north-central Utah. Remote sensing of chlorophyll as a monitoring and analysis method can provide a more spatially complete representation of algae distribution and biomass;information that is difficult to obtain using point samples.
文摘New methods of analysis for water quality monitoring to detect inorganic substances are required to meet the demands of determining concentration, particularly at low detection limits, analysing speciation and even identifying the pollution source. Such information is essential to inform public health decisions and to comply with more stringent legislation. This paper concentrates on two case studies, reviewing the development in monitoring methods, and predicting future trends. Arsenic and nitrates detection was selected as these pollutants are particularly problematic from a human health perspective. Additionally, the challenges faced in developing monitoring methods for these chemicals are relevant to a wide range of other inorganics. The current state of the art in detection approaches for these chemicals are discussed along with recommendations for future research to further improve the methods.