[Objective] The paper was to establish a HPLC method for quantitative determination of 80% pymetrozine·nitenpyram WG. [Method] With the mixture of acetonitrile, water and phosphate buffer （volume ratio 10：80...[Objective] The paper was to establish a HPLC method for quantitative determination of 80% pymetrozine·nitenpyram WG. [Method] With the mixture of acetonitrile, water and phosphate buffer （volume ratio 10：80：10） as the mobile phase, pymetrozine and nitenpyram in samples were separated by ODS-2 reversed phase column, and detected by UV detector at 250 nm. In addition, the results were quantified by external standard method. [Result] The linear correlation coefficients of pymetrozine and nitenpyram were 0.9995 and 0.9998; the standard deviations were 0.29 and 0.22; the coefficients of variation were 0.51% and 1.21%; and the average recoveries were 100.7% and 99.1%, respectively. [Conclusion] The method is fast and simple, and can effectively separate pymetrozine and nitenpyram, with high precision and accuracy and good linear relation.展开更多
文摘[Objective] The paper was to establish a HPLC method for quantitative determination of 80% pymetrozine·nitenpyram WG. [Method] With the mixture of acetonitrile, water and phosphate buffer （volume ratio 10：80：10） as the mobile phase, pymetrozine and nitenpyram in samples were separated by ODS-2 reversed phase column, and detected by UV detector at 250 nm. In addition, the results were quantified by external standard method. [Result] The linear correlation coefficients of pymetrozine and nitenpyram were 0.9995 and 0.9998; the standard deviations were 0.29 and 0.22; the coefficients of variation were 0.51% and 1.21%; and the average recoveries were 100.7% and 99.1%, respectively. [Conclusion] The method is fast and simple, and can effectively separate pymetrozine and nitenpyram, with high precision and accuracy and good linear relation.
