[Objectives]To investigate the effects of propolis extract on HeLa cells to provide theoretical guidance for facilitating clinical treatment.[Methods]The effects of propolis on inflammation,and proliferation were anal...[Objectives]To investigate the effects of propolis extract on HeLa cells to provide theoretical guidance for facilitating clinical treatment.[Methods]The effects of propolis on inflammation,and proliferation were analyzed based on the levels of DNA transcriptional regulation and mRNA and protein expression levels.[Results]Propolis water extract(PWE)could inhibit the cell proliferation and production of DNA damage in a dosedependent manner.Moreover,the propolis water extract could significantly downregulate the expression of iNOS-Luc,PTGS-2-Luc,and IL-8-Luc,and that it was related to the expression of the NF-κB family protein.After the induction of HeLa cells by propolis,the expression of the cell cycle inhibitor gene p21 was increased,while that of the cell proliferation gene Ki67 was decreased.[Conclusions]Propolis water extract could significantly inhibit the cell proliferation and production of DNA damage,suggesting propolis as a potential candidate for the development of adjunctive therapy against cervical cancer.展开更多
本文研究了季节和地理来源对中国蜂胶水提物的化学成分和抗氧化性的影响。分别采集了来自山东、湖北和海南三个省份不同季节的蜂胶,采用改进的提取方法,得到蜂胶水提物(Water extract of propolis,WEP)。分光光度法测定了WEP的主要化学...本文研究了季节和地理来源对中国蜂胶水提物的化学成分和抗氧化性的影响。分别采集了来自山东、湖北和海南三个省份不同季节的蜂胶,采用改进的提取方法,得到蜂胶水提物(Water extract of propolis,WEP)。分光光度法测定了WEP的主要化学组成,普鲁士兰法和清除DPPH自由基活性法评价了WEP的抗氧化性。研究结果表明,中国WEP含有总酚和总黄酮等化学成分,具有较好的抗氧化性。季节对中国WEP化学成分和抗氧化性有显著影响。山东和湖北WEP化学成分比较接近,但同海南WEP有显著差异,抗氧化活性也显著强于海南WEP。不同产地和不同采收季节的中国WEP存在化学组成及生物活性多样性,在生产实践中需注意这一特性。展开更多
为明确中国蜂胶水提物的抑菌活性,本文采用琼脂扩散法和微量液体稀释法,分别测定了来自中国26个地区的蜂胶水提物(Water Extraction of Propolis,WEP)对金黄色葡萄球菌、大肠杆菌、变形杆菌、产气杆菌和毛霉的抑菌圈和最小抑菌浓度。结...为明确中国蜂胶水提物的抑菌活性,本文采用琼脂扩散法和微量液体稀释法,分别测定了来自中国26个地区的蜂胶水提物(Water Extraction of Propolis,WEP)对金黄色葡萄球菌、大肠杆菌、变形杆菌、产气杆菌和毛霉的抑菌圈和最小抑菌浓度。结果表明:除云南西双版纳WEP外,其余WEP均对受试菌表现出一定的抑菌性。西南地区WEP对金黄色葡萄球菌抑菌性最强,东北地区WEP对毛霉抑菌性最强,华东地区WEP对大肠杆菌、变形杆菌和产气杆菌抑菌性均最强。中国蜂胶水提物具有一定的抑菌活性,对5种受试菌抑制程度由大到小为:金黄色葡萄球菌(6.71±2.97)mm>变形杆菌(6.48±2.89)mm>产气杆菌(6.3±2.78)mm>毛霉(3.57±3.38)mm>大肠杆菌(2.18±3.36)mm。展开更多
Propolis is a resinous substance collected by honeybees from various plant sources.On account of its chemical composition,propolis possesses several biologi-cal and pharmacological properties.In recent years,many pape...Propolis is a resinous substance collected by honeybees from various plant sources.On account of its chemical composition,propolis possesses several biologi-cal and pharmacological properties.In recent years,many papers have provided information concerning its composi-tion.This review compiles data from most studies of propolis,focusing on the chemical composition of ethanol extracts of propolis(EEP),water extracts of propolis(WEP),and volatile oils from propolis(VOP).The characteristic compounds of EEP are polyphenols includ-ing flavonoids and related phenolic acids,and flavonoids are the most abundant and effective parts.They are considered to contribute more to the antibacterial,antiviral,and antioxidant effects than the other constituents.The main flavonoids in EEP are pinocembrin,galangin,chrysin,quercetin,kaempferol,and naringenin.The constituents reported to be in WEP include phenolic acids,caffeoylquinic acid,3-mono-O-caffeoylquinic acid,caffeic acid,flavonoids,etc.The propolis volatile compounds are benzyl alcohol,benzyl acetate,cinnamic alcohol,vanillin,eudensmol,cyclohexyl benzoate,and benzyl benzoate,which are responsible for several biological properties.As a natural mixture,propolis is widely used in medicine and cosmetics,as well as being a constituent of health foods.Since propolis has been used extensively,information on its composition is not only of interest to the academic field,but also to propolis users.展开更多
基金Supported by the Modern Agricultural Technology Industry System of ShandongProvince(SDAIT-24-05)。
文摘[Objectives]To investigate the effects of propolis extract on HeLa cells to provide theoretical guidance for facilitating clinical treatment.[Methods]The effects of propolis on inflammation,and proliferation were analyzed based on the levels of DNA transcriptional regulation and mRNA and protein expression levels.[Results]Propolis water extract(PWE)could inhibit the cell proliferation and production of DNA damage in a dosedependent manner.Moreover,the propolis water extract could significantly downregulate the expression of iNOS-Luc,PTGS-2-Luc,and IL-8-Luc,and that it was related to the expression of the NF-κB family protein.After the induction of HeLa cells by propolis,the expression of the cell cycle inhibitor gene p21 was increased,while that of the cell proliferation gene Ki67 was decreased.[Conclusions]Propolis water extract could significantly inhibit the cell proliferation and production of DNA damage,suggesting propolis as a potential candidate for the development of adjunctive therapy against cervical cancer.
文摘本文研究了季节和地理来源对中国蜂胶水提物的化学成分和抗氧化性的影响。分别采集了来自山东、湖北和海南三个省份不同季节的蜂胶,采用改进的提取方法,得到蜂胶水提物(Water extract of propolis,WEP)。分光光度法测定了WEP的主要化学组成,普鲁士兰法和清除DPPH自由基活性法评价了WEP的抗氧化性。研究结果表明,中国WEP含有总酚和总黄酮等化学成分,具有较好的抗氧化性。季节对中国WEP化学成分和抗氧化性有显著影响。山东和湖北WEP化学成分比较接近,但同海南WEP有显著差异,抗氧化活性也显著强于海南WEP。不同产地和不同采收季节的中国WEP存在化学组成及生物活性多样性,在生产实践中需注意这一特性。
文摘为明确中国蜂胶水提物的抑菌活性,本文采用琼脂扩散法和微量液体稀释法,分别测定了来自中国26个地区的蜂胶水提物(Water Extraction of Propolis,WEP)对金黄色葡萄球菌、大肠杆菌、变形杆菌、产气杆菌和毛霉的抑菌圈和最小抑菌浓度。结果表明:除云南西双版纳WEP外,其余WEP均对受试菌表现出一定的抑菌性。西南地区WEP对金黄色葡萄球菌抑菌性最强,东北地区WEP对毛霉抑菌性最强,华东地区WEP对大肠杆菌、变形杆菌和产气杆菌抑菌性均最强。中国蜂胶水提物具有一定的抑菌活性,对5种受试菌抑制程度由大到小为:金黄色葡萄球菌(6.71±2.97)mm>变形杆菌(6.48±2.89)mm>产气杆菌(6.3±2.78)mm>毛霉(3.57±3.38)mm>大肠杆菌(2.18±3.36)mm。
基金This study was supported by the National Natural Science Foundation of China(Grant No.30700105)the Culture Program for Young Scientists of Jiangxi Provincial Department of Science and Technology(No.S00590).
文摘Propolis is a resinous substance collected by honeybees from various plant sources.On account of its chemical composition,propolis possesses several biologi-cal and pharmacological properties.In recent years,many papers have provided information concerning its composi-tion.This review compiles data from most studies of propolis,focusing on the chemical composition of ethanol extracts of propolis(EEP),water extracts of propolis(WEP),and volatile oils from propolis(VOP).The characteristic compounds of EEP are polyphenols includ-ing flavonoids and related phenolic acids,and flavonoids are the most abundant and effective parts.They are considered to contribute more to the antibacterial,antiviral,and antioxidant effects than the other constituents.The main flavonoids in EEP are pinocembrin,galangin,chrysin,quercetin,kaempferol,and naringenin.The constituents reported to be in WEP include phenolic acids,caffeoylquinic acid,3-mono-O-caffeoylquinic acid,caffeic acid,flavonoids,etc.The propolis volatile compounds are benzyl alcohol,benzyl acetate,cinnamic alcohol,vanillin,eudensmol,cyclohexyl benzoate,and benzyl benzoate,which are responsible for several biological properties.As a natural mixture,propolis is widely used in medicine and cosmetics,as well as being a constituent of health foods.Since propolis has been used extensively,information on its composition is not only of interest to the academic field,but also to propolis users.
