Water-soluble polysaccharide was isolated from grapefruit with the method of water-dissolving and ethanol-precipitating. By single factor test and orthogonal experimental method, the extraction conditions of the water...Water-soluble polysaccharide was isolated from grapefruit with the method of water-dissolving and ethanol-precipitating. By single factor test and orthogonal experimental method, the extraction conditions of the water-soluble polysaccharide were investigated, which contained liquid-solid ratio, temperature, time and pH. The results showed that pH was the most important factor of polysaccharide extraction, followed by temperature. The optimized extraction technology conditions were obtained. The liquid-solid ratio was 1:40, temperature was 90?C, time was 2.0 h, and pH was 2. The highest extraction rate of crude polysaccharide was 8.30%.展开更多
The incidence rate of colon cancer ranks the third among malignant tumors worldwide.Gloeostereum incarnatum(GI),a fungus with homology of medicine and food,has multiple pharmacological activities.It was investigated i...The incidence rate of colon cancer ranks the third among malignant tumors worldwide.Gloeostereum incarnatum(GI),a fungus with homology of medicine and food,has multiple pharmacological activities.It was investigated in this study that the anti-colon cancer effect of polysaccharides purifi ed from GI(GIPS)in ApcMinC/Gpt mice(an in situ colon cancer mouse model).Eight-week administration with GIPS at doses of 30 or 90 mg/kg strongly inhibited tumor growth including the reduction on numbers and the suppression of the size without infl uencing the animals’body weight and organ functions.According to the proteomics performing by antibody array,among 308 detected cytokines,GIPS significantly regulated 89 of them.Compared with vehicle-treated mice,GIPS effectively reduced the levels of interleukin(IL)-1β,IL-4,IL-6,IL-17,IL-22,tumor necrosis factor(TNF)-α,matrix metalloproteinase(MMP)-2,and enhanced the levels of IL-15 and IL-18 in serum and/or colon tissues,which suggested its anti-inflammation of GIPS.GIPS suppressed nuclear aggregation of β-catenin,affected the expression of WNT1 and related proteins,thereby regulated the activation of the Wnt signaling.Altogether,GIPS can inhibit the growth of colon cancer,at least partially,via inhibiting the Wnt/β-catenin signaling pathway.展开更多
The gel properties of soybean isolate protein(SPI)gels are susceptible to the influence of other components in food products,particularly edible polysaccharides.Therefore,six common edible polysaccharides were selecte...The gel properties of soybean isolate protein(SPI)gels are susceptible to the influence of other components in food products,particularly edible polysaccharides.Therefore,six common edible polysaccharides were selected in this study to investigate their effects on the rheological properties,thermal properties,and microstructure of acid-induced soybean isolate protein self-assembled gels(SPIASG).The experimental results showed that the support capacity and creep recovery of SPIASG with added polysaccharides were higher than those of the control group without added polysaccharides,and the gel with Xanthan gum(XG)was the most effective.The hybrid gel,with added Condensed resin(CR),Xanthan gum(XG),Carrageenan(CA),and CMC formed a stronger network structure.Additionally,compared with SPIASG,the hybrid gel with added edible polysaccharides the water-holding capacity of SPI hybrid gels was relatively improved.In this study,a simple and easy method was obtained to significantly improve the gel properties of SPIASG,analyzed and compared the effectiveness of various polysaccharides to enhance its gel properties,and provided some ideas to improve the gel properties of SPI protein gels.展开更多
Encapsulation of water-soluble nitrogen fertilizers by membranes can be used to control the release of nutrients to maximize the fertilization ef fect and reduce environmental pollution.In this research,we formulated ...Encapsulation of water-soluble nitrogen fertilizers by membranes can be used to control the release of nutrients to maximize the fertilization ef fect and reduce environmental pollution.In this research,we formulated a new double-coated controlled-release fertilizer(CRF)by using food-grade microcrystalline wax(MW)and marine polysaccharide derivatives(calcium alginate and chitosan-glutaraldehyde copolymer).The pellets of water-soluble nitrogen fertilizer were coated with the marine polysaccharide derivatives and MW.A convenient and eco-friendly method was used to prepare the CRF.Scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FTIR)were used to characterize the morphology and composition of the products.The nitrogen-release properties were determined in water using UV-Vis spectrophotometry.The controlled-release properties of the fertilizer were improved dramatically after coating with MW and the marine polysaccharide derivatives.The results show that the double-coated CRFs can release nitrogen in a controlled manner,have excellent controlled-release features,and meet the European Standard for CRFs.展开更多
乳液凝胶可用于替代部分脂肪,降低蛋黄酱等食品中饱和脂肪酸的含量,并作为递送生物活性物质的载体。采用热处理的大豆分离蛋白(soy protein isolate,SPI)和大豆种皮多糖(soybean hull polysaccharide,SHP)制备乳液凝胶,利用流变仪、傅...乳液凝胶可用于替代部分脂肪,降低蛋黄酱等食品中饱和脂肪酸的含量,并作为递送生物活性物质的载体。采用热处理的大豆分离蛋白(soy protein isolate,SPI)和大豆种皮多糖(soybean hull polysaccharide,SHP)制备乳液凝胶,利用流变仪、傅里叶变换红外光谱仪、X射线衍射仪、扫描电镜和质构仪分析不同SHP质量分数(0、0.15%、0.30%、0.45%、0.60%)对乳液凝胶结构、流变特性、凝胶性和乳化稳定性的影响。结果表明:随着SHP质量分数的增加,乳液凝胶的微观结构更加致密,储存稳定性、G′和G″值、相对回收率逐渐增加。随着SHP质量分数的增加,乳液凝胶的表观黏度增加,并在SHP质量分数为0.45%时,达到最大。红外光谱和扫描电子显微镜观察结果显示,SHP和SPI之间存在分子间相互作用和氢键作用。储藏实验结果证实,储存7 d后乳液凝胶的储藏稳定性随着SHP添加量的增加而增强,当SHP质量分数为0.45%时,乳液凝胶稳定性较高。研究旨在为SHP在食品乳液凝胶中的开发和应用提供理论依据。展开更多
文摘Water-soluble polysaccharide was isolated from grapefruit with the method of water-dissolving and ethanol-precipitating. By single factor test and orthogonal experimental method, the extraction conditions of the water-soluble polysaccharide were investigated, which contained liquid-solid ratio, temperature, time and pH. The results showed that pH was the most important factor of polysaccharide extraction, followed by temperature. The optimized extraction technology conditions were obtained. The liquid-solid ratio was 1:40, temperature was 90?C, time was 2.0 h, and pH was 2. The highest extraction rate of crude polysaccharide was 8.30%.
基金This work was supported by the Ministry of Science and Technology of the People’s Republic of China(Grant No.2018YFE0107800)the“13th Five-year”Science and Technology Projects from Education Department in Jilin Province of P.R.China(Grant No.JJKH20190108KJ)Industrial Technology Research and Development Projects from Development and Reform Commission of Jilin Province(Grant No.2019C050-8).
文摘The incidence rate of colon cancer ranks the third among malignant tumors worldwide.Gloeostereum incarnatum(GI),a fungus with homology of medicine and food,has multiple pharmacological activities.It was investigated in this study that the anti-colon cancer effect of polysaccharides purifi ed from GI(GIPS)in ApcMinC/Gpt mice(an in situ colon cancer mouse model).Eight-week administration with GIPS at doses of 30 or 90 mg/kg strongly inhibited tumor growth including the reduction on numbers and the suppression of the size without infl uencing the animals’body weight and organ functions.According to the proteomics performing by antibody array,among 308 detected cytokines,GIPS significantly regulated 89 of them.Compared with vehicle-treated mice,GIPS effectively reduced the levels of interleukin(IL)-1β,IL-4,IL-6,IL-17,IL-22,tumor necrosis factor(TNF)-α,matrix metalloproteinase(MMP)-2,and enhanced the levels of IL-15 and IL-18 in serum and/or colon tissues,which suggested its anti-inflammation of GIPS.GIPS suppressed nuclear aggregation of β-catenin,affected the expression of WNT1 and related proteins,thereby regulated the activation of the Wnt signaling.Altogether,GIPS can inhibit the growth of colon cancer,at least partially,via inhibiting the Wnt/β-catenin signaling pathway.
基金financially supported by the 2021 Postgraduate Research Ability Improvement Program BTBU,the Joint Program of Beijing Natural Science Foundation Committee and Beijing Education Committee(Grant No.KZ201810011017)Beijing Excellent Talent Training Project(Grant No.2017000020124G100).
文摘The gel properties of soybean isolate protein(SPI)gels are susceptible to the influence of other components in food products,particularly edible polysaccharides.Therefore,six common edible polysaccharides were selected in this study to investigate their effects on the rheological properties,thermal properties,and microstructure of acid-induced soybean isolate protein self-assembled gels(SPIASG).The experimental results showed that the support capacity and creep recovery of SPIASG with added polysaccharides were higher than those of the control group without added polysaccharides,and the gel with Xanthan gum(XG)was the most effective.The hybrid gel,with added Condensed resin(CR),Xanthan gum(XG),Carrageenan(CA),and CMC formed a stronger network structure.Additionally,compared with SPIASG,the hybrid gel with added edible polysaccharides the water-holding capacity of SPI hybrid gels was relatively improved.In this study,a simple and easy method was obtained to significantly improve the gel properties of SPIASG,analyzed and compared the effectiveness of various polysaccharides to enhance its gel properties,and provided some ideas to improve the gel properties of SPI protein gels.
基金Supported by the National Natural Science Foundation of China(No.41306071)the CAS STS Program(No.KFJ-SW-STS-143)+1 种基金the NSFC-Shandong Union Project(No.U1406402-5)the Public Science and Technology Research Funds Projects of Ocean(Nos.201405038-2,201305016-2)
文摘Encapsulation of water-soluble nitrogen fertilizers by membranes can be used to control the release of nutrients to maximize the fertilization ef fect and reduce environmental pollution.In this research,we formulated a new double-coated controlled-release fertilizer(CRF)by using food-grade microcrystalline wax(MW)and marine polysaccharide derivatives(calcium alginate and chitosan-glutaraldehyde copolymer).The pellets of water-soluble nitrogen fertilizer were coated with the marine polysaccharide derivatives and MW.A convenient and eco-friendly method was used to prepare the CRF.Scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FTIR)were used to characterize the morphology and composition of the products.The nitrogen-release properties were determined in water using UV-Vis spectrophotometry.The controlled-release properties of the fertilizer were improved dramatically after coating with MW and the marine polysaccharide derivatives.The results show that the double-coated CRFs can release nitrogen in a controlled manner,have excellent controlled-release features,and meet the European Standard for CRFs.
