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Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain
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作者 Gao GAO Xing LI +7 位作者 Kangxiang QIN Yun HU Xiaosong JIANG Chenxi CHE Yuntao LI Changkao MU Chunlin WANG Huan WANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第4期1336-1347,共12页
The farming of Scylla paramamosain with specific flavors has a higher commercial value,and the flavors are related to the integrated farming environment and non-volatile flavor substances,while the survival environmen... The farming of Scylla paramamosain with specific flavors has a higher commercial value,and the flavors are related to the integrated farming environment and non-volatile flavor substances,while the survival environment is one of the important ways to source gut microorganisms in the organism.In this study,the levels of dominant taxa in the gut flora of S.paramamosain from Mong Cai,Vietnam(VN),Taishan City,Guangdong Province(TS)of China,and Ninghai County,Ningbo City(NB)Zhejiang Province of China converged with those of S.paramamosain from Sanmen County,Ningbo City(CK 1,CK 2,and CK 3)at 28 d of domestication.The top 15 genera with the highest abundance of VN,TS,and NB gut flora were the same as CK 1,CK 2,and CK 3,but with different percentages,and gradually converged to CK 1,CK 2,and CK 3,respectively,at 28 d of domestication.Correlation between intestinal flora and non-volatile flavor substances in the hepatopancreas at the percentage level of relative abundance of bacterial genera found that above 28 d of domestication,Muribaculaceae,Psychrilyobacter,Clostridia_vadinBB 60_group,Halarcobacter Carboxylicivirga,Sediminispirochaeta may be the most important genera affecting flavor amino acids of VN.Sediminispirochaeta,Carboxylicivirga,Halarcobacter,Photobacterium,ZOR 0006,Psychrilyobacter,and Pseudomonas may be the most important genera affecting flavor amino acids of NB.Sediminispirochaeta,Carboxylicivirga,Halarcobacter,Photobacterium,ZOR 0006,Vibrio,and Sphingomonas may be the most important genera affecting flavor amino acids of TS.These results show that the intestinal flora structure of crabs from different areas were domesticated in the same area for at least 28 d before they converged to that of the domesticated crab,and the most important genera affecting the flavor amino acids of TS,VN,and NB were also identified.The results of this study provide a reference and basis for the technique of directional cultivation of the flavor quality of the crab. 展开更多
关键词 survival environment Scylla paramamosain intestinal flora structure non-volatile flavor substance directional breeding
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Occurrence,Spatial Distribution,Sources and Risk Assessment of Per-and Polyfluoroalkyl Substances in Surface Sediments of the Yellow River Delta Wetland
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作者 SUN Yu SHEN Nan +5 位作者 ZHANG Dahai CHEN Junhui HE Xiuping JI Yinli WANG Haiyang LI Xianguo 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第5期1263-1274,共12页
Per-and polyfluoroalkyl substances(PFASs)are emerging persistent organic pollutants(POPs).In this study,47 surface sediment samples were collected from the Yellow River Delta wetland(YRDW)to investigate the occurrence... Per-and polyfluoroalkyl substances(PFASs)are emerging persistent organic pollutants(POPs).In this study,47 surface sediment samples were collected from the Yellow River Delta wetland(YRDW)to investigate the occurrence,spatial distribution,potential sources,and ecological risks of PFASs.Twenty-three out of 26 targeted PFASs were detected in surface sediment samples from the YRDW,with totalΣ23PFASs concentrations ranging from 0.23 to 16.30 ng g^(-1) dw and a median value of 2.27 ng g^(-1) dw.Perfluorooctanoic acid(PFOA),perfluorobutanoic acid(PFBA)and perfluorooctanesulfonic acid(PFOS)were the main contaminants.The detection frequency and concentration of perfluoroalkyl carboxylic acids(PFCAs)were higher than those of perfluoroal-kanesulfonic acids(PFSAs),while those of long-chain PFASs were higher than those of short-chain PFASs.The emerging PFASs substitutes were dominated by 6:2 chlorinated polyfluoroalkyl ether sulfonic acid(6:2 Cl-PFESA).The distribution of PFASs is significantly influenced by the total organic carbon content in the sediments.The concentration of PFASs seems to be related to human activities,with high concentration levels of PFASs near locations such as beaches and villages.By using a positive matrix factorization model,the potential sources of PFASs in the region were identified as metal plating mist inhibitor and fluoropolymer manufacturing sources,metal plating industry and firefighting foam and textile treatment sources,and food packaging material sources.The risk assessment indicated that PFASs in YRDW sediments do not pose a significant ecological risk to benthic organisms in the region overall,but PFOA and PFOS exert a low to moderate risk at individual stations. 展开更多
关键词 per-and polyfluoroalkyl substances Yellow River Delta wetland sediment source identification risk assessment
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Research Progress of Volatile Substances Analysis and Sensory Evaluation of Soymilk
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作者 Xu Qiongyuan Li Ka +7 位作者 Huang Meina Liang Yuxin Tao Bingjie Zhang Tingting Song Nannan Ji Yazhou Zhang Huajiang Xia Ning 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第1期74-82,共9页
Soybean is one of the important crops in China. Soymilk, a traditional neutral plant-based protein drink, is rich in high quality proteins. Although soybean milk is rich in nutrients, its marketing among consumers, es... Soybean is one of the important crops in China. Soymilk, a traditional neutral plant-based protein drink, is rich in high quality proteins. Although soybean milk is rich in nutrients, its marketing among consumers, especially those in Western countries who are used to peaceful flavor, has been limited due to the adverse flavor impact brought by its special composition. In recent years, with the increasing attention to the nutritional value of soymilk, the flavor of soymilk has become a popular research object for scholars at home and abroad. The flavor components of soymilk are mainly volatile small molecular compounds produced by enzymatic reactions catalyzed by lipoxygenase(LOX). After formation, they interact with protein macromolecules to form the overall flavor of soymilk. At present, there are many methods to control the off-odor of soymilk at home and abroad, including physical heating methods, chemical methods, biological enzymatic digestion methods, mask methods, and a variety of breeding methods. These methods effectively reduce the off-odor of soymilk, but all of them have shortcomings. Currently, the sensory characteristics of the beany odor in soymilk are evaluated mainly by traditional human sensory scoring along with the assistance of modern instrument analysis of volatile flavor substances using headspace solid phase microextraction(SPME) gas chromatography coupled with-mass spectrometry(GC-MS). This paper summarized the research results of volatile flavor substances in soymilk in recent years and the sensory evaluation methods of soymilk at home and abroad, and looked forward to the future development direction, hoping to provide some theoretical bases and reference detection methods for solving the problem of soymilk flavor in the future. 展开更多
关键词 SOYMILK off-odor volatile substance FLAVOR sensory evaluation
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Evaluation of Rhizobium tropici-Derived Extracellular Polymeric Substances on Selected Soil Properties, Seed Germination, and Growth of Black-Eyed Peas (Vigna unguiculata)
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作者 Jonathan Alunge Metuge Erneste Havugimana +2 位作者 Jean Rugandirababisha Zachary N. Senwo Marie Chantal Mutimawurugo 《Agricultural Sciences》 2024年第5期548-564,共17页
Rhizobium tropici-derived extracellular polymeric substances (EPS) have been used in soils to enhance soil structures and mitigate soil erosions. However, information on their use to improve soil health and fertility ... Rhizobium tropici-derived extracellular polymeric substances (EPS) have been used in soils to enhance soil structures and mitigate soil erosions. However, information on their use to improve soil health and fertility indicators, and plant growth is limited. In a greenhouse study, we investigated their effects on some soil health, soil fertility indices, and the growth of black-eyed peas (Vigna unguiculate). Results showed that soils incubated with EPS significantly increased basal soil respiration, soil microbial biomass, permanganate oxidizable carbon (POC), and potentially mineralizable nitrogen (PMN). The EPS shifted microbial populations from bacteria to fungi and Gram (−ve) to Gram ( ve) bacteria. However, it had little or no effects on soil pH, soil organic matter (SOM), and cation exchange capacity (CEC). The EPS decreased soil moisture loss, increased soil aggregate stability, but delayed blacked-eyed peas germinations in the soils. At 0.1% (w/w) concentrations in soils, there was increase in plant root nodulations and vegetative growth. This study was carried out within 40 days of incubating soils with EPS or growing the black-eyed peas in a greenhouse study. The plant growth parameters were taken before flowering and fruiting. Further studies of the effects of incubating soils with the extracellular polymeric substances on plant growth. Soil microbial biomass, microbial diversities, and other soil fertility indices are deemed necessary. 展开更多
关键词 Rhizobium tropici Extracellular Polymeric substances Soil Respiration Soil Microbial Biomass Black-Eyed Peas
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Contemporary strategies and approaches for characterizing composition and enhancing biofilm penetration targeting bacterial extracellular polymeric substances
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作者 Lan Lu Yuting Zhao +4 位作者 Mingxing Li Xiaobo Wang Jie Zhu Li Liao Jingya Wang 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2024年第4期506-524,共19页
Extracellular polymeric substances(EPS)constitutes crucial elements within bacterial biofilms,facili-tating accelerated antimicrobial resistance and conferring defense against the host's immune cells.Developing pr... Extracellular polymeric substances(EPS)constitutes crucial elements within bacterial biofilms,facili-tating accelerated antimicrobial resistance and conferring defense against the host's immune cells.Developing precise and effective antibiofilm approaches and strategies,tailored to the specific charac-teristics of EPS composition,can offer valuable insights for the creation of novel antimicrobial drugs.This,in turn,holds the potential to mitigate the alarming issue of bacterial drug resistance.Current analysis of EPS compositions relies heavily on colorimetric approaches with a significant bias,which is likely due to the selection of a standard compound and the cross-interference of various EPS compounds.Considering the pivotal role of EPS in biofilm functionality,it is imperative for EPS research to delve deeper into the analysis of intricate compositions,moving beyond the current focus on polymeric materials.This ne-cessitates a shift from heavy reliance on colorimetric analytic methods to more comprehensive and nuanced analytical approaches.In this study,we have provided a comprehensive summary of existing analytical methods utilized in the characterization of EPS compositions.Additionally,novel strategies aimed at targeting EPS to enhance biofilm penetration were explored,with a specific focus on high-lighting the limitations associated with colorimetric methods.Furthermore,we have outlined the challenges faced in identifying additional components of EPS and propose a prospective research plan to address these challenges.This review has the potential to guide future researchers in the search for novel compounds capable of suppressing EPS,thereby inhibiting biofilm formation.This insight opens up a new avenue for exploration within this research domain. 展开更多
关键词 Analytic strategies and approaches Composition characterization Extracellular polymeric substances(EPS) Promoting biofilm penetration
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Preserving the Ozone Layers: Battling Illegal Trade in Ozone-Depleting Substances
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作者 Ibrahim Badawi 《Atmospheric and Climate Sciences》 2024年第3期287-298,共12页
The depletion of the ozone layer, a vital shield protecting the Earth from harmful ultraviolet (UV) radiation, is now a worldwide environmental concern. Human activities, particularly the release of ozone depleting su... The depletion of the ozone layer, a vital shield protecting the Earth from harmful ultraviolet (UV) radiation, is now a worldwide environmental concern. Human activities, particularly the release of ozone depleting substances (ODS), have led to the thinning of this protective layer over recent decades. Simultaneously, illegal trade has emerged as a global challenge, giving rise to economic issues, losses of tax revenue, heightened criminal activities, health risks, and environmental hazards. The depletion of the ozone layer, a critical shield protecting the Earth from harmful ultraviolet (UV) radiation, has become a global environmental concern. This paper delves into the legal dimensions surrounding ozone-depleting substances (ODS), their impact on the ozone layer, and the subsequent risk of skin cancer. As countries navigate international agreements, domestic regulations, and enforcement mechanisms, the intricate interplay between legal frameworks and the health implications of ozone layer depletion comes to the forefront. The paper highlights particular instances of illegal trade in ozone depleting substances, drawing from data reported by the parties to the Montreal Protocol. Notably, China stands out as a significant source of contraband ODS, with other countries such as Bulgaria, Lithuania, Poland, and France reporting numerous cases. Analyzing these case instances offers insights into the efficacy of legal frameworks and enforcement measures. The paper offers a comprehensive set of recommendations to strengthen global control and enforcement against the illegal trade of ozone depleting substances. These recommendations span diverse aspects such as production monitoring, customs collaboration, mutual verification, cross-border agreements, public-private partnerships, international cooperation, detection equipment, global regulatory standards, resource allocation, public awareness campaigns, alternative substance development, and controlling the trade at its source. By applying these recommendations and enhancing enforcement measures, we aim to protect the ozone layer and create a healthier and safer world for future generations and achieve sustainable development goals. 展开更多
关键词 Ozone-Depleting substances Illegal Trade Montreal Protocol Environmental Protection Ozone Layers Climate Change
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The Role of Jasmonates as Antibulbing Substances in the Bulb Formation of Onion
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作者 Noboru Takada Atsushi Saito +4 位作者 Yuuki Matsuzuka Tatsushi Mochiduki Eriko Wakita Meng WANG Yasunori Koda 《Agricultural Biotechnology》 2024年第1期1-4,8,共5页
Onion plants form spherical bulbs under long-day conditions.Substances regulating bulb formation remain unknown.In the course of chemical studies on the bulb formation,α-linolenic acid was isolated from onion extract... Onion plants form spherical bulbs under long-day conditions.Substances regulating bulb formation remain unknown.In the course of chemical studies on the bulb formation,α-linolenic acid was isolated from onion extracts as an antibulbing substance,the amount of which was synchronized with the bulb formation.Since allene oxide synthase inhibitor canceled the antibulbing activity ofα-linolenic acid,it was disclosed that jasmonic acid concerns this regulation.Structure-activity-relationship study revealed that its(3R,7S)stereochemistry is necessary for showing its antibulbing activity.It is concluded that(3R,7S)-jasmonate derived fromα-linolenic acid actually participates in the regulation of bulb formation. 展开更多
关键词 Onion(Allim cepa L.cv.Higuma) ISOLATION Bulb formation Antibulbing substance α-Linolenic acid Methyl jasmonate
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Analyzing the pharmacological substances and targets of Xuefu Zhuyu decoction in hypertensive vascular endothelial cells
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作者 Rui-Xue Chen Jing Li +3 位作者 Guo-Zhen Dong Sheng-Yan Qiao Xiao Hu Li-Guo Chen 《Clinical Research Communications》 2024年第1期3-10,共8页
Background:Xuefu Zhuyu decoction(XFZY)could significantly improve the function of hypertensive vascular endothelial cells,but the targets and mechanism are not clear.This study is to analyze the pharmacological substa... Background:Xuefu Zhuyu decoction(XFZY)could significantly improve the function of hypertensive vascular endothelial cells,but the targets and mechanism are not clear.This study is to analyze the pharmacological substances and targets of Xuefu Zhuyu decoction in hypertensive vascular endothelial cells.Methods:This study used Xuefu Zhuyu decoction to intervene human umbilical vein endothelial cells incubated by hypertensive patients’serum,then detected the function of vascular endothelial cells.The aqueous extract of XFZY was analyzed and validated by liquid chromatography-mass spectrometry technology;Finally,macromolecular docking technology was used to analyze the potential active substances and targets of XFZY in the prevention and treatment of hypertension.Results:Compared with the model group,the XFZY group showed a significant increase in NO expression(P<0.01)and a significant decrease in ET-1 expression(P<0.001);and the expression of BIP,P-JNK,CHOP,and BAX in XFZY group cells was significantly decreased(P<0.001),while the expression of JNK and BCL2 was significantly increased(P<0.001).19 main compounds were identified in XFZY and there were 3 pairs of molecular complexes with high affinity for markers of the endoplasmic reticulum stress,including BIP-Hesperidin complex,BIP-HSYA complex and JNK-Naringin complex.Conclusion:This study analyzed the potential pharmacodynamic substance and targets of Xuefu Zhuyu decoction in improving the function of hypertensive vascular endothelial cells,which could provide a scientific basis for the future molecular mechanism of XFZY in treating hypertension. 展开更多
关键词 Xuefu Zhuyu decoction HYPERTENSION vascular endothelial cells pharmacological substances and targets
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Advances in Research Methods of Pharmacodynamic Substances in Traditional Chinese Medicines and Their Application in the Teaching of Traditional Chinese Medicine Analysi
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作者 Jiangwei Tian 《Journal of Contemporary Educational Research》 2024年第5期97-102,共6页
With the continuous progress of science and technology,the research methods of pharmacodynamic substances in traditional Chinese medicine are developing,and the application of these methods in teaching is becoming mor... With the continuous progress of science and technology,the research methods of pharmacodynamic substances in traditional Chinese medicine are developing,and the application of these methods in teaching is becoming more and more extensive.By introducing these research methods into the classroom,teachers can help students to deeply understand the nature and mechanism of action of pharmacodynamic substances in traditional Chinese medicine,and improve their interest in and knowledge of traditional Chinese medicine.This paper introduces the definition of pharmacodynamic substances in traditional Chinese medicine,research methods,and their application in the teaching of traditional Chinese medicine analysis. 展开更多
关键词 Traditional Chinese medicine Pharmacodynamic substances Teaching of traditional Chinese medicine analysis Teaching application
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Study on Water-Soluble Organic Reducing Substances: II. Organic Reducing Substances Produced from Anaerobic Decomposition of Milk Vetch (Astragalus sinicus L.) 被引量:1
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作者 WUYOU-XIAN DINGCHANG-PU 《Pedosphere》 SCIE CAS CSCD 1992年第4期355-362,共8页
The characteristics of electric charge and molecular weight distribution,oxidation-reduction regimes,e.g.Eh and amounts of organic reducing substances produced by milk vetch during anaerobic decomposition process,were... The characteristics of electric charge and molecular weight distribution,oxidation-reduction regimes,e.g.Eh and amounts of organic reducing substances produced by milk vetch during anaerobic decomposition process,were studied by using electrochemical methods.Interaction between soils and organic reducing substances was also observed.The results indicate that the organic reducing substances were mainly the organic compounds with negative and amphoteric charges,which were distributed in two groups at anodic peak potentials of 0.25 and 0.69 volt in differential pulse voltammograms,respectively.Their apparent molecular weights are all less than 700 daltons,in which those active in oxidation-reducion reaction were distributed in the fraction with apparent molecular weight less than 200 daltons.The organic reduction substances can be oxidized by manganese oxides in their interaction with soils. 展开更多
关键词 electric charge milk vetch molecular weight organic reducing substances oxidation-reduction regime
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Study on Water-Soluble Organic Reducing Substances. I. Determination of Organic Reducing Substances by Differential Pulse Voltammetry
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作者 WUYOU-XIAN DINGCHANG-PU 《Pedosphere》 SCIE CAS CSCD 1991年第2期157-167,共11页
A new method was proposed for study of organic reducing substances in soils. According to the theoretical relationship between the voltammetric behaviors and reduction-oxidation reaction of reducing substances, the wo... A new method was proposed for study of organic reducing substances in soils. According to the theoretical relationship between the voltammetric behaviors and reduction-oxidation reaction of reducing substances, the working conditions of differential pulse voltammetry (d.p. v.) for determining the organic reducing substances produced during the processes of the anaerobic decomposition of plant materials were established with a glass carbon electrode as working electrode, 1 M Ag-AgCl electrode with large area as reference electrode, 0.2 M NH4AC as supporting electrolyte and pH buffer solution, pulse amplitude (AE) of 25 mV, scan rate at 2 mV·S-1and scan potential ranging from -0.5 to +1.2 voltage(vs. M Ag-AgCl). The peak current proportional to the concentration of reducing substances, and the characteristic peak potential of each organic reducing substance were regarded as the quantitative and qualitative base, respectively. These results obtained under the conditions mentioned above directly reflect both the reducing intensity and capacity of the organic reducing system in soils. 展开更多
关键词 differential pulse voltammetry organic reducing substances peak current peak potential
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Comparative Evaluation of Humic Substances: Effect at Cell Level and Chlorophyll Retention during Accelerated Senescence
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作者 Tirichy Ganesh Prasad Joz Liza +2 位作者 Bargur Jayaram Manjushree Ramarao Nagalakshmi Kumar Devendra 《American Journal of Plant Sciences》 2023年第6期638-652,共15页
The influence of humic substances (HS) formulations derived from sedimentary and compost sources was studied on plant growth at cell level and chlorophyll retention during accelerated senescence of leaf tissue. The di... The influence of humic substances (HS) formulations derived from sedimentary and compost sources was studied on plant growth at cell level and chlorophyll retention during accelerated senescence of leaf tissue. The direct effect of HS formulations was studied on cell expansion using cucumber and radish cotyledon expansion test. The cucumber hypocotyl elongation test was used to study the effect on cell elongation. Chlorophyll pigment retention in excised leaf tissue incubated in dark with high temperature was assessed to study the effect on leaf senescence. Explant tissues were incubated directly in the solutions of the formulations at the concentration recommended for foliar application to the crop plants. HS formulations showed significant variations in their direct bio-stimulatory effects. Formulations derived from compost sources were found superior in terms of inducing a direct stimulatory effect on cell expansion and cell elongation and in maintaining chlorophyll pigment retention during accelerated senescence. HS from sedimentary sources stimulated cell expansion and delayed chlorophyll degradation to a lesser extent compared to HS from compost. However, HS formulations derived from sedimentary sources used in this study were not effective in inducing cell elongation in the cucumber hypocotyl elongation test. The direct bio-stimulatory effect of HS formulations differed significantly between the formulations that were evaluated. 展开更多
关键词 Biostimulants CHLOROPHYLL COMPOST COTYLEDON Humic substances HYPOCOTYL SEDIMENTARY
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Effect of extracellular polymeric substances on Dolichospermum aggregation during temperature rise
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作者 Dailan DENG Han MENG +6 位作者 You MA Yongqi GUO Zixuan WANG Huan HE Khan WAQAS Jin’e LIU Limin ZHANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第6期2208-2218,共11页
Dolichospermum,a typical model filamentous of cyanobacteria,has the potential to cause severely bloom.Extracellular polymeric substances(EPSs)are considered to influence the aggregation of the algae,and temperature is... Dolichospermum,a typical model filamentous of cyanobacteria,has the potential to cause severely bloom.Extracellular polymeric substances(EPSs)are considered to influence the aggregation of the algae,and temperature is a significant factor affecting EPSs secretion.However,the mechanism of how EPSs affects the aggregation of Dolichospermum is still unclear because the structure and composition of EPSs are complex.In this study,the effects of EPSs on the aggregation of Dolichospermum during the rise of temperature(7-37℃)were determined.The results showed that the concentration of extracellular polysaccharides and proteins changed significantly with increasing temperature(P<0.01).Firstly,during the increasing temperature,the polysaccharide content of EPSs increased from 20.34 to 54.64 mg/L,and the polysaccharides in the soluble EPS(S-EPS)layer changed significantly.The protein content reached maximum value at 21℃(14.52 mg/L)and varied significantly in S-EPS and loosely bound EPS(LB-EPS).In the EPSs matrix,humus substances and protein were main components of S-EPS and LB-EPS,and protein was the main component of tightly bound EPS(TB-EPS).Secondly,the cell density of Dolichospermum increased during the temperature rise while the aggregation ratio decreased.Moreover,zeta potential and surface thermodynamic analysis of Dolichospermum revealed that the interfacial free energy and electrostatic repulsion increased gradually with increasing temperature,which further reduced the aggregation of Dolichospermum.Finally,principal component analysis(PCA)analysis showed the aggregation of Dolichospermum was directly related to the changes of protein in EPSs(especially S-EPS and LB-EPS)and zeta potential,and polysaccharides in EPSs inhibited the aggregation of Dolichospermum.Based on these results,it was illustrated that the composition and concentration of EPSs affected the cell surface properties of Dolichospermum with the change of temperature and thus affected the aggregation of Dolichospermum. 展开更多
关键词 TEMPERATURE Dolichospermum extracellular polymeric substances AGGREGATION
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Role of extracellular polymeric substances in resistance to allelochemical stress on Microcystis aeruginsosa and its mechanism
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作者 Li YIN Ying XU +7 位作者 Desheng KONG Juan WANG Kaipian SHI Yong ZHANG Huan HE Shaogui YANG Lixiao NI Shiyin LI 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第6期2219-2231,共13页
Using allelochemicals to suppress cyanobacteria growth is a prospective method for its high efficiency and ecological safety.However,the suppression efficiency is affected inevitably by the extracellular polymeric sub... Using allelochemicals to suppress cyanobacteria growth is a prospective method for its high efficiency and ecological safety.However,the suppression efficiency is affected inevitably by the extracellular polymeric substances(EPS)produced by cyanobacteria,and the knowledge about the roles of EPS in resistance to allelochemical stress is scarce.For the study,two typical anti-cyanobacterial allelochemicals were adopted to investigate the role of EPS in resistance to allelochemical stress on Microcystis aeruginosa.Results show that EPS was crucial in alleviating the toxicity of allelochemicals to algae,especially in stabilizing the metabolism and photosynthetic activity of algal cells.The aggregation rate of algal cells increased with the increase of EPS secretion,which alleviated the stress of allelopathy.Tryptophan proteins and humic acids in EPS provided a binding site for allelochemicals,and the EPS-allelochemicals complex were formed by chemical bonding.This study improved our comprehension of the role of EPS in algal inhibition by allelochemicals. 展开更多
关键词 ALLELOCHEMICALS extracellular polymeric substances CYANOBACTERIA Microcystis aeruginosa ALLELOPATHY
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Chemical substances present in discharge water generated by laundry industry:Analytical monitoring
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作者 Dario Lacalamita Chiara Mongioví Grégorio Crini 《Water Science and Engineering》 EI CAS CSCD 2023年第4期324-332,共9页
To our knowledge,precise data concerning the pollution in terms of qualitative and quantitative fluctuations in discharge water from the laundry sector have seldom been reported.This study investigated the chemical co... To our knowledge,precise data concerning the pollution in terms of qualitative and quantitative fluctuations in discharge water from the laundry sector have seldom been reported.This study investigated the chemical composition of the discharge water from a laundry industry.Over 160 chemical substances and 15 standard water parameters were monitored.The results showed that the discharge water presented both inorganic and organic polycontamination with a high degree of qualitative and quantitative variability.However,of all monitored substances,only five metals(Al,Cu,Fe,Sr,and Zn),five minerals(P,Ca,K,Na,and S),and alkylphenols were systematically present and quantifiable.For a daily average water flow of 129 m^(3),the released metal flux was 356 g/d.Substances,such as trichloromethane,brominated diphenyl ether(BDE)47,and fluorides,were occasionally found and quantified.Other substances,such as chlorophenols,organo-tins,and pesticides were never identified.All the samples had quantifiable levels in the chemical oxygen demand(COD),biological oxygen demand(BOD),and hydrocarbons.Only the concentrations of Zn(8.3 g/d),Cu(21.4 g/d),and BOD(57.4 g/d)were close to or above the regulatory values:74.0 g/d for Zn,9.0 g/d for Cu,and 57.0 kg/d for BOD.The data obtained from this study are useful to the choice of additional treatments for the reduction of pollutant fluxes. 展开更多
关键词 LAUNDRY Discharge water Chemical analysis Water parameter Chemical substance
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A sutureless technique for securing leaking sclerotomies with viscoelastic substances in 23-gauge microincision vitrectomy surgery
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作者 Meng Li Quan-Yong Yi +4 位作者 Jing-Hai Mao Yan-Hong Liao Yan-Yan Wang Qin-Kang Lu Yan Gong 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第5期730-735,共6页
AIM:To introduce and evaluate the clinical efficacy of a new technique,the use of viscoelastic substances(VS)to close leaking sclerotomy in 23G microincision vitrectomy,and to observe its effect on the visual acuity a... AIM:To introduce and evaluate the clinical efficacy of a new technique,the use of viscoelastic substances(VS)to close leaking sclerotomy in 23G microincision vitrectomy,and to observe its effect on the visual acuity and intraocular pressure(IOP)of patients.METHODS:Patients who underwent 23G vitrectomy in Ningbo Eye Hospital before the use of VS technique(June 2019 to September 2020)and after the use of VS technique(October 2020 to December 2021)were selected as the subjects of this study.The above cases underwent operation by the same surgeon and were retrospectively analyzed.VS technique was used as the alternative to suturing,in which a small amount of VS was injected at the leaking sclerotomy and then gently massaged to confirm leaking sclerotomy closure.RESULTS:A total of 174 eyes were covered in the study,including 84 eyes in the control group(before the use of VS technique)and 90 eyes in the VS technique group.The number of eyes that needed to be sutured decreased considerably from 42.9%in the control group to 3.3%in the VS technique group,and the proportion of subconjunctival hemorrhage at 1-2d after surgery decreased remarkably from 35.7%in the control group to 2.2%in the VS technique group.No substantial differences in the incidence of mean IOP and low IOP were found between 1-2 and 3-20d after surgery in the VS technique group.No major complications associated with VS technique were identified during the study.CONCLUSION:In 23G microincision vitrectomy,VS technique is a safe,simple,and effective method to close leaking sclerotomy. 展开更多
关键词 vitrectomy surgery leaking sclerotomy 23-gauge sutureless technique viscoelastic substances intraocular pressure
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Could school programs based on social-emotional learning prevent substance abuse among adolescents?
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作者 João Mauricio Castaldelli-Maia Nicolas Kohatsu Matakas 《World Journal of Psychiatry》 SCIE 2024年第8期1143-1147,共5页
In this editorial,we comment on the article Adolescent suicide risk factors and the integration of social-emotional skills in school-based prevention programs by Liu et al.While the article focused on the issue of sui... In this editorial,we comment on the article Adolescent suicide risk factors and the integration of social-emotional skills in school-based prevention programs by Liu et al.While the article focused on the issue of suicide and social-emotional learning programs as a possible intervention,we here discuss evidence of other reported outcomes and if it could be an effective way to prevent substance abuse among adolescents. 展开更多
关键词 Social-emotional learning substance use ADOLESCENT Student Prevention Mental health
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Relationship and effect of redox potential,jarosites and extracellular polymeric substances in bioleaching chalcopyrite by acidithiobacillus ferrooxidans 被引量:13
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作者 余润兰 钟代立 +3 位作者 苗雷 吴发登 邱冠周 顾国华 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2011年第7期1634-1640,共7页
The changes of pH,redox potential,concentrations of soluble iron ions and Cu^2+ with the time of bioleaching chalcopyrite concentrates by acidithiobacillus ferrooxidans were investigated under the different condition... The changes of pH,redox potential,concentrations of soluble iron ions and Cu^2+ with the time of bioleaching chalcopyrite concentrates by acidithiobacillus ferrooxidans were investigated under the different conditions of initial total-iron amount as well as mole ratio of Fe(III) to Fe(II) in the solutions containing synthetic extracellular polymeric substances (EPS).When the solution potential is lower than 650 mV (vs SHE),the inhibition of jarosites to bioleaching chalcopyrite is not vital as EPS produced by bacteria can retard the contamination through flocculating jarosites even if concentration of Fe(III) ions is up to 20 g/L but increases with increasing the concentration of Fe(III) ions;jarosites formed by bio-oxidized Fe3+ ions are more easy to adhere to outside surface of EPS space on chalcopyrite;the EPS layer with jarosites acts as a weak diffusion barrier to further rapidly create a high redox potential of more than 650 mV by bio-oxidizing Fe^2+ ions inside and outside EPS space into Fe^3+ ions,resulting in a rapid deterioration of ion diffusion performance of the EPS layer to inhibit bioleaching chalcopyrite severely and irreversibly. 展开更多
关键词 extracellular polymeric substances CHALCOPYRITE BIOLEACHING jarosites redox potential
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Interaction mechanism of Cu^(2+),Fe^(3+) ions and extracellular polymeric substances during bioleaching chalcopyrite by Acidithiobacillus ferrooxidans ATCC2370 被引量:11
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作者 余润兰 刘晶 +5 位作者 陈安 钟代立 李乾 覃文庆 邱冠周 顾帼华 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第1期231-236,共6页
The extracellular polymeric substances(EPS) of Acidithiobacillus ferrooxidans ATCC 23270,and iron and copper enclosed in EPS were extracted by ultrasonication and centrifugation methods to determine the interaction ... The extracellular polymeric substances(EPS) of Acidithiobacillus ferrooxidans ATCC 23270,and iron and copper enclosed in EPS were extracted by ultrasonication and centrifugation methods to determine the interaction mechanism of Cu2+,Fe3+ and EPS during bioleaching chalcopyrite.Generally,Cu2+ ions can stimulate bacteria to produce more EPS than Fe3+ ions.The mass ratio of Fe3+/Cu2+ enclosed in EPS decreased gradually from about 4:1 to about 2:1 when the concentration of Cu2+ ions increased from 0.01 to 0.04 mol/L.The amount of iron and copper bound together by EPS in ferrous-free 9K medium containing 1% chalcopyrite was about 2 times of that in 9K medium containing 0.04 mol/L Cu2+ ions.It was inferred that the EPS with jarosites on the surface of chalcopyrite gradually acted as a weak diffusion barrier for Cu2+,Fe3+ ions transference during bioleaching chalcopyrite. 展开更多
关键词 extracellular polymeric substances iron ions copper ions BIOLEACHING CHALCOPYRITE
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Study on the Effect of Osmosis-regulating Substances and Organic Appendices on Somatic Embryogenesis in Wheat 被引量:4
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作者 孙朝霞 侯思宇 王玉国 《Agricultural Science & Technology》 CAS 2008年第3期46-48,共3页
[Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat. [Method] The suitable concentration combination of appendices was optimized b... [Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat. [Method] The suitable concentration combination of appendices was optimized by adding different concentrations of osmosis-regulating substances including mannitol, sorbitol and organic appendices such as Gln, CH and LH, into the somatic embryogenesis in wheat. [Result] The mannitol or sorbitol lower than 40 g/L was helpful for improving somatic embryogenesis; there was no significant difference in the induction rate of somatic embryogenesis when 300-500 mg/L Gln、CH or LH was respectively added into the induced medium, while somatic embryogenesis could be enhanced dramatically in the presence of 500 mg/L Gln together with 300 mg/L CH. [Conclusion] Somatic embryogenesis could be improved to some extent by different concentrations of osmosis-regulating substances and organic appendices, which laid foundation for establishing a more perfect system of somatic embryogenesis in wheat. 展开更多
关键词 WHEAT SOMATIC EMBRYOGENESIS ORGANIC appendix Osmosis-regulating substance
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