The study aims at exploring the possibility of using the recovery ability af- ter drought stress-rewatering at vegetative growth stage as the evaluating index in water use efficiency (WUE) of winter wheat varieties....The study aims at exploring the possibility of using the recovery ability af- ter drought stress-rewatering at vegetative growth stage as the evaluating index in water use efficiency (WUE) of winter wheat varieties. 'Jing 411 ', 'Jinmai 47' and their 34 near isogenic lines (NILs) were used as test materials. Semi-automatic rainproof shelter and the percolating pools were used for simulating drought treat- ment. After suffering severe drought stress, winter wheat crops were rewatered at early jointing stage. The biomass accumulation after rewatering was determined as recovery ability index. In the meanwhile, plant height in the end of vegetative growth stage was measured, and WUE of varieties/lines was also determined. Thereafter, the differences in recovery ability, plant height and the population WUE, together with the correlation between recovery ability and population WUE were analyzed, respectively. The results showed that there were significant differences in recovery ability among some varieties/lines. The recovery ability was affected by both geno- type and environment, and the interaction existed in these two factors. Significant differences existed in plant height and population WUE among the 34 NILs along with their parents. There was a significantly positive correlation between recovery ability and plant height of varieties/lines. Recovery ability and plant height were very significantly and positively correlated with population yield WUE respectively. The re- sults indicated that recovery ability after drought stress-rewatering could be used as an evaluating index of population WUE under drought condition.展开更多
As part of a larger project to examine the richness and distribution of wildlife in Kumtag Desert area, we conducted camera trapping surveys during the period 2010–2012 at seven watering sites in an arid region of th...As part of a larger project to examine the richness and distribution of wildlife in Kumtag Desert area, we conducted camera trapping surveys during the period 2010–2012 at seven watering sites in an arid region of the Altun Mountains in western China. Information on activity patterns of the wild bactrian camel (Camelus ferus), kiang (Equus kiang), goitered gazelle (Gazella subgutturosa), argali (Ovis ammon), blue sheep (Pseudois nayaur), red fox (Vulpes vulpes), and wolf (Canis lupus) was obtained. We found that the wild camel, kiang, goitered gazelle, argali, and blue sheep were predominantly diurnal at watering sites, whereas red fox and wolf were nocturnal. Five herbivores partitioned the use of watering sites in a temporal manner to minimize the risk of predation by carnivores. The wild camel was the dominant herbivorous species at the watering sites. The kiang, goitered gazelle, argali, and blue sheep displayed adaptive water use by altering spatial or temporal patterns based on the presence or absence of wild camel, to minimize the risk of interspecific strife. These results are suggestive of the differences in activity patterns that might modulate water partitioning by different species, and provide insights for the development of conservation strategies for integrated species and decisions regarding water development in the Altun Mountains.展开更多
In this study, we developed a computer program for automatic prediction of watering time point by considering the environmental factors such as solar radiation, air temperature and relative humidity based on the multi...In this study, we developed a computer program for automatic prediction of watering time point by considering the environmental factors such as solar radiation, air temperature and relative humidity based on the multiple linear regression equation of leaf area and Penman Method. The experiments were carried out for a year in two watering experimental plots, one of which was controlled by pF value, and the other by the computer program. After comparing the results of the two plots, the following findings were obtained. In the computer program plot, the observed and predicted values of both leaf area and evapotranspiration indicated significant correlation at the 1% level, which suggested that the computer program had high prediction accuracy. In addition, no significant difference was observed between the two experimental plots with respects to the plant height, plant diameter, leaf area, leaf number, fresh weight, and dry weight, which demonstrated that the plants in the computer program plot had normal growth. On the other hand, although the number of flower buds and flowering shoots showed higher values at the end of certain cultivations in the computer program plot than those in pF value plot, we proposed that it was due to the effect of cumulative daily solar radiation in the greenhouse, rather than the watering. Thus, we have reached the conclusion that the computer program for automatic prediction of watering time point developed by this study has high applicability in miniature pot rose production.展开更多
After water stress at various levels and durations at different growth stages, rewatering could greatly stimulate the leaf area development of winter wheat. The results showed that the stimulation effect changed with ...After water stress at various levels and durations at different growth stages, rewatering could greatly stimulate the leaf area development of winter wheat. The results showed that the stimulation effect changed with water stress time, degree and duration. Rewatering under earlier stress had greater stimulation effect on leaf area than that under later stress. Higher stimulation effect was observed under severe water stress than that under moderate stress. Longer duration of stress resulted in low stimulation effect. In spite of the greater stimulation effect under severe and longer stress, the final leaf area in these situations was lower than that under moderate stress and shorter duration. Whenever the stress occurred, the stimulating effect was due to the increase of the leaf area of the tillers. Once the leaf on the main stem emerged during stress period, rewatering had no effect on its size, and consequently its leaf area. The stimulation of rewateirng on leaf area contributed to the final grain yield by 45% under moderate stress, and 67% under severe stress. Although the stimulation partly compensated for the loss during stress, the final leaf area and the grain yield could not reach the level without water stress.展开更多
Market gardening, in the swampy lowlands of the Dschangcity, plays an important role in terms of providing employment, and supply the city with fresh products. However, waterborne diseases, which occur in the city, ar...Market gardening, in the swampy lowlands of the Dschangcity, plays an important role in terms of providing employment, and supply the city with fresh products. However, waterborne diseases, which occur in the city, are both attributed to the bad quality of drinking water and sanitation practices. This work aims to characterize watering waters of crop, to assess the health risks associated with the use of these waters. Water from eleven streams and five wells used to irrigate crops were sampled and analyzed once a week for a month. Analyses focused on physico-chemical parameters (temperature, suspended matter, pH, electrical conductivity, COD, BOD5, NO-3 , Al, Fe, Cu, Ni), parasitological (helminthes eggs) and bacteriological (total coliforms, fecal coliforms, fecal streptococcus). The results show that, the physico-chemical quality of well waters generally approaches the WHO standards of crop watering water. The physico-chemical of streams waters and bacteriological quality of wells and streams waters are bad, according to WHO guide. These bad quality waters could contaminate crops, some of which are eaten raw, which is surely a cause of the outbreak of waterborne diseases in the city. The waters from streams are more affected. The pretreatment of the water before use for irrigation of vegetables is highly recommended.展开更多
The effectiveness of three pre-germination treatments in breaking dormancy of the seeds of Dialum guineense and effects of three watering regimes on the growth performance of the seedlings were investigated. The pre-g...The effectiveness of three pre-germination treatments in breaking dormancy of the seeds of Dialum guineense and effects of three watering regimes on the growth performance of the seedlings were investigated. The pre-germination treatments were: soaking in hot water for 10 seconds and cooling down in cold water (T1), soaking in cold water for 24 hours (T2), soaking in running water for 48 hours (T3) and control (T4), while watering regimes include: watering once daily in the morning (W1), watering once every two days in the morning (W2) and watering once every three days in the morning (W3). The results showed that germination occurred first at 5 days after sowing (5 DAS) among the seeds soaked in cold water for 24 hours before sowing (T2), while the untreated seeds (control) took the longest period of 12 days before germination occurred. T1 had the highest germination value of 49.6% while T3 had the least of 31.2%. The effects of watering regimes were found to be significantly different on stem-collar diameter, leaf area and total dry weight (P < 0.05). The least significant difference (LSD) test showed that W2 and W3 supported the best growth performance. It is concluded from the results that T2 should be adopted for breaking the dormancy of Dialium guineense seeds and watering interval of three days would not dispose the seedlings to water-stress.展开更多
With an increase of mining the upper limits under unconsolidated aquifers, dewatering of the bottom aquifer of the Quaternary system has become a major method to avoid water and sand inrushes.In the 8th District of th...With an increase of mining the upper limits under unconsolidated aquifers, dewatering of the bottom aquifer of the Quaternary system has become a major method to avoid water and sand inrushes.In the 8th District of the Taiping Coal Mine in south-western Shandong province, the bottom aquifer of the Quaternary system is moderate to excellent in water-yielding capacity.The base rock above the coal seam is very thin in the concealed coal field of the Carboniferous and Permian systems.Therefore, a comprehensive dewatering plan from both the ground surface and the panel was proposed to lower the groundwater level in order to ensure mining safety.According to the hydrogeologic conditions of the 8th District, we established a numerical model so that we could simulate the groundwater flow in the dewatering process.We obtained the simulation parameters from previous data using backward modeling, such as the average coefficient of permeability of 12 m/d and the elastic storage coefficient of 0.002.From the same model, we predicted the movement of groundwater and water level variables and obtained the visible effect of the dewatering project.Despite the overburden failure during mining, no water and/or sand inrush occurred because the groundwater level in the bottom aquifer was lowered to a safe water level.展开更多
Among all fruit crops of horticultural importance, grapevines (Vitis vinifera L.) stand out as the most drought tolerant crop species whose tolerance is credited to their proficiency to recover from water stress in bo...Among all fruit crops of horticultural importance, grapevines (Vitis vinifera L.) stand out as the most drought tolerant crop species whose tolerance is credited to their proficiency to recover from water stress in both the natural and vineyard growing conditions. However, information on the recovery responses is relatively scant. Studies were conducted to address this issue using potted vines of the grapevine cultivar, Cabernet Sauvignon, which was subjected to water stress and along with anatomical and ultrastructural characterizations, physiological status was assessed in healthy and water stressed vines, and following recovery via rewatering from the water stressed vines. Water stress induced wilting of leaves, drooping of tendrils, and desiccation followed by abscission of shoot tip leaving behind a brown scar at the shoot apex. The wilted leaves accumulated ABA, which correspondingly reduced stomatal conductance and leaf water potential. Upon re-watering, both these parameters made a recovery with values similar to healthy leaves. Likewise, leaf anatomical features following rewatering resembled to that of healthy leaves. In clusters, water stress caused shriveling of preveraison (unripened) berries, which regained full turgor following water resupply, whereas the postveraison (ripening) berries in the same cluster remained unaffected as evidenced by the presence of viable mesocarp cells and epicuticular wax in the form of platelets. The study revealed that shoot tip with leaf primordia was most sensitive to water stress followed by fully expanded leaves and preveraison berries, whereas the postveraison berries remained unaffected. This information could be valuable to implementing irrigation strategies towards sustaining grape production in existing vineyards experiencing episodic droughts and targeted areas prone to drought.展开更多
[ Objective] To investigate the effects of different water amount on freezing point of raw milk. EMetbodl The freezing point of raw milk added water at different proportions was detected by SWC-LG freezing point detec...[ Objective] To investigate the effects of different water amount on freezing point of raw milk. EMetbodl The freezing point of raw milk added water at different proportions was detected by SWC-LG freezing point detector. The linear regression equation was obtained. The reproduc- ibility was checked. The water content in raw milk was calculated according to the standard regression curve. E Result~ When the water content changed from 0 to 10%, the freezing point of raw milk increased linearly. As evidenced by the reproducibility test, the standard deviation and rela- tive standard deviation were less than 1%. The milk concentration obtained by detecting the freezing point of blind milk samples had an absolute er- ror of no more than 0.5%, compared with actual watering milk concentration. [ ConcluMonl Water content in raw milk can be determined by measur- ing freezing point, and this method is reliable and reproducible.展开更多
Water is an important factor affecting growth, yield and distribution of different species. Plant response to water deficit can be in the form of physiological disorders, such as reduction in transpiration or assimila...Water is an important factor affecting growth, yield and distribution of different species. Plant response to water deficit can be in the form of physiological disorders, such as reduction in transpiration or assimilating partitioning to root growth. Sustainable use of water has become a priority in agriculture and thus innovative irrigation management practices are critical. The study aimed at investigating how watering frequency and terminal water stress influence growth of Pelargonium sidoides, an important medicinal plant in Southern Africa. The trial was a randomized complete block design with three replicates, and treatment factors were watering frequency (everyday, twice and once a week) and terminal water stress (no watering four weeks before harvesting, no watering two weeks before harvesting and no terminal stress). There was an interacting effect of watering frequency and terminal water stress on biomass and fresh root yield. More frequent watering resulted in significantly higher biomass and fresh root yield, compared to other treatments. Watering everyday with terminal or no terminal water stress resulted in higher fresh root yield, compared to other watering treatments with terminal water stress. Plant height and leaf area were significantly affected by watering frequency and terminal water stress, respectively. A significant drop in stomatal conductance of plants watered everyday was observed 240 d after treatment implementation, such that there was no significant difference across all the three watering frequency treatments. In conclusion, farmers can save on irrigation costs by reducing watering frequency, as there was no significant difference on dry root yield.展开更多
In this study, a magnetic micro-particle conditioning–pressurized vertical electro-osmotic dewatering(MPEOD) process with magnetic micro-particle conditioning–drainage under gravity–mechanical compression–electr...In this study, a magnetic micro-particle conditioning–pressurized vertical electro-osmotic dewatering(MPEOD) process with magnetic micro-particle conditioning–drainage under gravity–mechanical compression–electrical compression(MMPC–DG–MC–EC) stages was established to study the distribution and migration of water, extracellular polymeric substances(EPS), and other organic matter in the activated sludge(AS) matrix at each stage.Results showed that the MPEOD process could attain 53.52% water content(WC) in dewatered AS with bound water(BW) and free water(FW) reduction rates of 82.97% and 99.67%,respectively. The coagulation and time-delayed magnetic field effects of magnetic microparticles(MMPs) along the MMPC–DG–MC stages initiated the transformation of partial BW to FW in AS. EC had a coupling driving effect of electro-osmosis and pressure on BW, and the changes in pH and temperature at EC stage induced the aggregation of AS flocs and the release of partial BW. Additionally, MMPs dosing further improved the dewatering performance of AS by acting as skeleton builders to provide water passages. Meanwhile, MMPs could disintegrate sludge cells and EPS fractions, thereby reducing tryptophan-like protein and byproduct-like material concentrations in LB-EPS as well as protein/polysaccharide ratio in AS matrix, which could improve AS filterability. At EC stage, the former four Ex/Em regions of fluorescence regional integration analysis for EPS were obviously reduced, especially the protein-like substances in LB-and TB-EPS, which contributed to improvement of AS dewaterability.展开更多
This paper presents a case study on groundwater control and environmental protection during a deep excavation of the foundation pit for the Liyang Road Station of Metro Line 10 in Shanghai.A three-dimensional finite e...This paper presents a case study on groundwater control and environmental protection during a deep excavation of the foundation pit for the Liyang Road Station of Metro Line 10 in Shanghai.A three-dimensional finite element simulation model was constructed to quantify the effects of a dewatering process on the environment around the excavation pit.To understand the decrease in the groundwater level around the foundation pit,the ground settlement and groundwater level were studied.During the excavation,environmental protection methods were used in the design of the optimal watering system to check its detrimental environmental effects.The effects of the diaphragm wall and horizontal barrier system were analyzed by varying their insertion depths and the resulting changes in the ground settlement and groundwater level were studied.It was found that increasing the insertion depth of the diaphragm wall reduced settlement near the excavation site but increased the construction costs.The diaphragm wall used in combination with a horizontal barrier was found to be effective in arresting the decrease in groundwater level and reducing settlement around the excavation site.展开更多
Due to large-scale dredging operations, a large amount of sludge is inevitably produced. Large areas of land are occupied when the dredged sludge is discarded in the disposal site as waste material. The sludge dewater...Due to large-scale dredging operations, a large amount of sludge is inevitably produced. Large areas of land are occupied when the dredged sludge is discarded in the disposal site as waste material. The sludge dewatering with aeration-vacuum (SDAV) method is suit for treating the sludge with high water content and high clay content in the disposal site. The water in the sludge can be discharged out. The volume of the sludge can be reduced quickly, and the recycling of the land can be accelerated by this method. Most importantly, this technique is an efficient way to deal with clogging problems when pumping water from high water content, high clay content dredged sludge. Vacuum degree range tests, the aeration rate range tests, and the influencing factors of sludge dewatering behavior tests were conducted with a self-developed SDAV model test device. Sludge samples were taken from the South-to-North Water Diversion East Line Project in Huai’an White-Horse Lake disposal site, Jiangsu Province, China. The optimal range of vacuum degree and aeration rate were obtained through the test results, and the mechanisms for how the two factors work and how they affect the sludge dewatering behavior were analyzed. The suitable vacuum degree range in SDAV is below 50 kPa, and the suitable aeration rate is about 1.0 m3/h. The low-vacuum degree contributes to reduce the ad-sorption effect of micro-channels on soil particles in filter material and to maintain the arch structures. Aeration has the effects of expansion, disturbance, changing Reynolds number, and dynamic sieve separating. The pump quantity of water per meter of filter tube (m) has different change rules as the vacuum degree changes under different aeration rates. The reason is that the formed arch structures’ conformation and permeability differ greatly under different combined-conditions of vacuum degree and aeration rate. The optimal combined-condition for dewatering the sludge is 35 kPa with 1.0 m3/h.展开更多
Cu-Mn co-doped CeO_(2) photocatalyst was successfully synthesized by the sol-gel method to assess its capability in degrading tetracycline.XRD and TEM results showed that Cu and Mn were successfully co-doped into CeO_...Cu-Mn co-doped CeO_(2) photocatalyst was successfully synthesized by the sol-gel method to assess its capability in degrading tetracycline.XRD and TEM results showed that Cu and Mn were successfully co-doped into CeO_(2) without forming heterostructure,XPS and photoelectrochemical results revealed that Mn ions doping amplified the generation of photo-induced charge carriers,while Cu ions doping significantly facilitated the interfacial charge transfer process.Notably,the optimized Cu3Mn2CeO_(2) nanoparticles exhibited the highest TC removal efficiency,achieved a rate of 78.18%and maintained a stable cycling performance.展开更多
The available test methods for optimal moisture content of cold recycled mixture(CRM)as well as its bulk specific gravity,and theoretical maximum relative density were analyzed in this work.Some test improvements were...The available test methods for optimal moisture content of cold recycled mixture(CRM)as well as its bulk specific gravity,and theoretical maximum relative density were analyzed in this work.Some test improvements were suggested to improve test control of the CRM road performance based on the discovered flaws.Besides,the properties of reclaimed asphalt pavement(RAP),including the content of old asphalt,penetration index,passing rate of 4.75 mm sieve,and gradation change rate after extraction,were examined.The effects of RAP characteristics on splitting tensile strength,water stability,the high-and low-temperature performance of emulsified asphalt CRM were studied.The results show that the optimum moisture content of CRM should be determined when the compaction work matches the specimen’s molding work.Among the analyzed methods of bulk specific gravity assessment,the dry-surface and CoreLok methods provide more robust and accurate results than the wax-sealing method,while the dry-surface method is the most cost-efficient.The modified theoretical maximum relative density test method is proposed,which can reduce the systematic error of the vacuum test method.The following RAP-CRM trends can be observed.The lower the content of old asphalt and the smaller the change rate of gradation,the smaller the voids and the better the water stability of CRM.The greater the penetration of old asphalt,the higher the fracture work and low-temperature splitting strength.The greater the penetration,the higher the passing rate of 4.75 mm sieve after extraction,and the worse the high-temperature performance of CRM.展开更多
Green hydrogen from water splitting has emerged as a critical energy vector with the potential to spearhead the global transition to a fossil fuel-independent society.The field of catalysis has been revolutionized by ...Green hydrogen from water splitting has emerged as a critical energy vector with the potential to spearhead the global transition to a fossil fuel-independent society.The field of catalysis has been revolutionized by single-atom catalysts(SACs),which exhibit unique and intricate interactions between atomically dispersed metal atoms and their supports.Recently,bimetallic SACs(bimSACs)have garnered significant attention for leveraging the synergistic functions of two metal ions coordinated on appropriately designed supports.BimSACs offer an avenue for rich metal–metal and metal–support cooperativity,potentially addressing current limitations of SACs in effectively furnishing transformations which involve synchronous proton–electron exchanges,substrate activation with reversible redox cycles,simultaneous multi-electron transfer,regulation of spin states,tuning of electronic properties,and cyclic transition states with low activation energies.This review aims to encapsulate the growing advancements in bimSACs,with an emphasis on their pivotal role in hydrogen generation via water splitting.We subsequently delve into advanced experimental methodologies for the elaborate characterization of SACs,elucidate their electronic properties,and discuss their local coordination environment.Overall,we present comprehensive discussion on the deployment of bimSACs in both hydrogen evolution reaction and oxygen evolution reaction,the two half-reactions of the water electrolysis process.展开更多
The effects of liquid-solid ratio and reaction time on the leaching rate of magnesium at room temperature were investigated,as well as the effects of the molar ratio of MgO/MgCl_(2),the amount of water added,and the a...The effects of liquid-solid ratio and reaction time on the leaching rate of magnesium at room temperature were investigated,as well as the effects of the molar ratio of MgO/MgCl_(2),the amount of water added,and the amount of acid-impregnated slag dosed on the compressive strength and water resistance of LR-MOC.The results showed that the magnesium element in the boron mud could be maximally leached under the conditions of 1:1 concentration of hydrochloric acid at room temperature,liquid-solid ratio of 2.5 mL·g^(-1),and reaction time of 5 h,and the main products were amorphous SiO_(2) as well as a small amount of magnesium olivine which had not been completely reacted.The LR-MOC prepared using the acid-soaked mixture could reach a softening coefficient of 0.85 for 28 d of water immersion when the molar ratio of MgO/MgCl_(2) was 2.2,the amount of water added was 0 g,and the acid-soaked slag dosing was 40 wt%,which also led to an appreciable late-strength,with an increase of 19.4%in compressive strength at 28 d compared to that at 7 d.Unlike previous studies,LR-MOC prepared in this way has a final strength phase that is not the more easily hydrolysed 3-phase but the lath-like 5-phase.For this phenomenon,we analyzed the mechanism and found that,during the acid leaching process,a part of amorphous SiO_(2) dissolved in the acid leaching solution formed a silica sol,in which Mg^(2+)played a bridging role to make the silica sol more stable.With the addition and hydrolysis of MgO,the silica sol gel coagulation slows down,providing a capping layer to inhibit the hydrolysis of the 5-phase crystals and providing some strength after coagulation.The amorphous SiO_(2) in the other part of the acid-impregnated slag generated M-S-H gel with Mg^(2+)and OH-,which synergised with the dense structure composed of interlocking crystals to improve the water resistance of LR-MOC.展开更多
The Janus fabrics designed for personal moisture/thermal regulation have garnered significant attention for their potential to enhance human comfort.However,the development of smart and dynamic fabrics capable of mana...The Janus fabrics designed for personal moisture/thermal regulation have garnered significant attention for their potential to enhance human comfort.However,the development of smart and dynamic fabrics capable of managing personal moisture/thermal comfort in response to changing external environments remains a challenge.Herein,a smart cellulose-based Janus fabric was designed to dynamically manage personal moisture/heat.The cotton fabric was grafted with N-isopropylacrylamide to construct a temperature-stimulated transport channel.Subsequently,hydrophobic ethyl cellulose and hydrophilic cellulose nanofiber were sprayed on the bottom and top sides of the fabric to obtain wettability gradient.The fabric exhibits anti-gravity directional liquid transportation from hydrophobic side to hydrophilic side,and can dynamically and continuously control the transportation time in a wide range of 3–66 s as the temperature increases from 10 to 40℃.This smart fabric can quickly dissipate heat at high temperatures,while at low temperatures,it can slow down the heat dissipation rate and prevent the human from becoming too cold.In addition,the fabric has UV shielding and photodynamic antibacterial properties through depositing graphitic carbon nitride nanosheets on the hydrophilic side.This smart fabric offers an innovative approach to maximizing personal comfort in environments with significant temperature variations.展开更多
In order to prevent the inrushing caused by deep excavations, dewatering measure has to be adopted to decrease the confined water level. In this study, the responses of the strata and supporting system to dewatering i...In order to prevent the inrushing caused by deep excavations, dewatering measure has to be adopted to decrease the confined water level. In this study, the responses of the strata and supporting system to dewatering in deep excavations are investigated through numerical simulations and case studies. Coupled fluid-mechanical analyses are performed by the use of the numerical software, FLAC3 D. The responses of the ground settlement,base heave and interior columns to the excavation and dewatering are analyzed. Numerical results indicate that the dewatering measure can effectively reduce the uplift of the subsurface soil in the excavation, and decrease the vertical displacement of the supporting system. In addition, field data of two case histories show the similar responses and confirm the validation of the numerical results. Based on the analyses, dewatering in the confined aquifer is recommended as a construction method for controlling the vertical displacement of the strata and supporting system in deep excavations.展开更多
Background:Previously,a chronic social defeat stress(CSDS)model has been widely-adopted for assessing depressive-like behaviors in animals.However,there is still room for improvement in the CSDS model to safeguard stu...Background:Previously,a chronic social defeat stress(CSDS)model has been widely-adopted for assessing depressive-like behaviors in animals.However,there is still room for improvement in the CSDS model to safeguard study accuracy and the wel-fare of lab rodents.Our study team developed a novel,standardized apparatus to induce CSDS in rodents and assessed the model's practical adaptability.Methods:An innovative CSDS cage apparatus and water bottle was designed.To evaluate the effectiveness of the newly developed tools,a variety of animal models,including the tail suspension test(TST),sucrose preference test,forced swimming test(FST),novelty-suppressed feeding test,female urine sniffing test,and open field test(OFT),were adopted to assess depressive-like behaviors in mice.Fluoxetine treat-ment was also administered to observe the reversal effect,as part of the validation.Results:The CSDS cage apparatus resulted in the manifestation of depressive-like behaviors in the model mice.Significant reductions in sucrose preference and urine sniffing time were observed,while the OFT revealed decreased central zone total distance,residence time,and frequency of entry.Moreover,increased immobility was found in the FST and TST.Fluoxetine treatment was found to successfully reverse the modeling effect.Conclusion:The CSDS cage apparatus was validated for enhanced usability and ad-dressed the previous challenges of water bottle leakage and lab rodent welfare is-sues.The consistent results from multiple behavioral tests also supported real-world application of the apparatus,offering researchers a promising alternative to conven-tional rodent cages.展开更多
文摘The study aims at exploring the possibility of using the recovery ability af- ter drought stress-rewatering at vegetative growth stage as the evaluating index in water use efficiency (WUE) of winter wheat varieties. 'Jing 411 ', 'Jinmai 47' and their 34 near isogenic lines (NILs) were used as test materials. Semi-automatic rainproof shelter and the percolating pools were used for simulating drought treat- ment. After suffering severe drought stress, winter wheat crops were rewatered at early jointing stage. The biomass accumulation after rewatering was determined as recovery ability index. In the meanwhile, plant height in the end of vegetative growth stage was measured, and WUE of varieties/lines was also determined. Thereafter, the differences in recovery ability, plant height and the population WUE, together with the correlation between recovery ability and population WUE were analyzed, respectively. The results showed that there were significant differences in recovery ability among some varieties/lines. The recovery ability was affected by both geno- type and environment, and the interaction existed in these two factors. Significant differences existed in plant height and population WUE among the 34 NILs along with their parents. There was a significantly positive correlation between recovery ability and plant height of varieties/lines. Recovery ability and plant height were very significantly and positively correlated with population yield WUE respectively. The re- sults indicated that recovery ability after drought stress-rewatering could be used as an evaluating index of population WUE under drought condition.
基金funded by National Nonprofit Institute Research Grant of Chinese Academy of Forestry (CAFYBB2011003)the Administrative Bureau of Xinjiang Lop Nur Wild Camel National Nature Reservethe Administrative Bureau of Gansu Annanba Wild Camel National Nature Reserve for their support of this study
文摘As part of a larger project to examine the richness and distribution of wildlife in Kumtag Desert area, we conducted camera trapping surveys during the period 2010–2012 at seven watering sites in an arid region of the Altun Mountains in western China. Information on activity patterns of the wild bactrian camel (Camelus ferus), kiang (Equus kiang), goitered gazelle (Gazella subgutturosa), argali (Ovis ammon), blue sheep (Pseudois nayaur), red fox (Vulpes vulpes), and wolf (Canis lupus) was obtained. We found that the wild camel, kiang, goitered gazelle, argali, and blue sheep were predominantly diurnal at watering sites, whereas red fox and wolf were nocturnal. Five herbivores partitioned the use of watering sites in a temporal manner to minimize the risk of predation by carnivores. The wild camel was the dominant herbivorous species at the watering sites. The kiang, goitered gazelle, argali, and blue sheep displayed adaptive water use by altering spatial or temporal patterns based on the presence or absence of wild camel, to minimize the risk of interspecific strife. These results are suggestive of the differences in activity patterns that might modulate water partitioning by different species, and provide insights for the development of conservation strategies for integrated species and decisions regarding water development in the Altun Mountains.
基金supported by the Science Founda-tion of Guangxi Zhuang Autonomous Region, China(0832002)
文摘In this study, we developed a computer program for automatic prediction of watering time point by considering the environmental factors such as solar radiation, air temperature and relative humidity based on the multiple linear regression equation of leaf area and Penman Method. The experiments were carried out for a year in two watering experimental plots, one of which was controlled by pF value, and the other by the computer program. After comparing the results of the two plots, the following findings were obtained. In the computer program plot, the observed and predicted values of both leaf area and evapotranspiration indicated significant correlation at the 1% level, which suggested that the computer program had high prediction accuracy. In addition, no significant difference was observed between the two experimental plots with respects to the plant height, plant diameter, leaf area, leaf number, fresh weight, and dry weight, which demonstrated that the plants in the computer program plot had normal growth. On the other hand, although the number of flower buds and flowering shoots showed higher values at the end of certain cultivations in the computer program plot than those in pF value plot, we proposed that it was due to the effect of cumulative daily solar radiation in the greenhouse, rather than the watering. Thus, we have reached the conclusion that the computer program for automatic prediction of watering time point developed by this study has high applicability in miniature pot rose production.
基金National Fundamental Research and Development (No. G1999011709 ) the National Natural Science Foundation (No.49971042).
文摘After water stress at various levels and durations at different growth stages, rewatering could greatly stimulate the leaf area development of winter wheat. The results showed that the stimulation effect changed with water stress time, degree and duration. Rewatering under earlier stress had greater stimulation effect on leaf area than that under later stress. Higher stimulation effect was observed under severe water stress than that under moderate stress. Longer duration of stress resulted in low stimulation effect. In spite of the greater stimulation effect under severe and longer stress, the final leaf area in these situations was lower than that under moderate stress and shorter duration. Whenever the stress occurred, the stimulating effect was due to the increase of the leaf area of the tillers. Once the leaf on the main stem emerged during stress period, rewatering had no effect on its size, and consequently its leaf area. The stimulation of rewateirng on leaf area contributed to the final grain yield by 45% under moderate stress, and 67% under severe stress. Although the stimulation partly compensated for the loss during stress, the final leaf area and the grain yield could not reach the level without water stress.
文摘Market gardening, in the swampy lowlands of the Dschangcity, plays an important role in terms of providing employment, and supply the city with fresh products. However, waterborne diseases, which occur in the city, are both attributed to the bad quality of drinking water and sanitation practices. This work aims to characterize watering waters of crop, to assess the health risks associated with the use of these waters. Water from eleven streams and five wells used to irrigate crops were sampled and analyzed once a week for a month. Analyses focused on physico-chemical parameters (temperature, suspended matter, pH, electrical conductivity, COD, BOD5, NO-3 , Al, Fe, Cu, Ni), parasitological (helminthes eggs) and bacteriological (total coliforms, fecal coliforms, fecal streptococcus). The results show that, the physico-chemical quality of well waters generally approaches the WHO standards of crop watering water. The physico-chemical of streams waters and bacteriological quality of wells and streams waters are bad, according to WHO guide. These bad quality waters could contaminate crops, some of which are eaten raw, which is surely a cause of the outbreak of waterborne diseases in the city. The waters from streams are more affected. The pretreatment of the water before use for irrigation of vegetables is highly recommended.
文摘The effectiveness of three pre-germination treatments in breaking dormancy of the seeds of Dialum guineense and effects of three watering regimes on the growth performance of the seedlings were investigated. The pre-germination treatments were: soaking in hot water for 10 seconds and cooling down in cold water (T1), soaking in cold water for 24 hours (T2), soaking in running water for 48 hours (T3) and control (T4), while watering regimes include: watering once daily in the morning (W1), watering once every two days in the morning (W2) and watering once every three days in the morning (W3). The results showed that germination occurred first at 5 days after sowing (5 DAS) among the seeds soaked in cold water for 24 hours before sowing (T2), while the untreated seeds (control) took the longest period of 12 days before germination occurred. T1 had the highest germination value of 49.6% while T3 had the least of 31.2%. The effects of watering regimes were found to be significantly different on stem-collar diameter, leaf area and total dry weight (P < 0.05). The least significant difference (LSD) test showed that W2 and W3 supported the best growth performance. It is concluded from the results that T2 should be adopted for breaking the dormancy of Dialium guineense seeds and watering interval of three days would not dispose the seedlings to water-stress.
基金Projects 40372123, 40772192 supported by the National Natural Science Foundation of ChinaNCET-04-0486 by the Program for New Century Excellent Talents in University of China2007CB209400 by the National Basic Research Program of China
文摘With an increase of mining the upper limits under unconsolidated aquifers, dewatering of the bottom aquifer of the Quaternary system has become a major method to avoid water and sand inrushes.In the 8th District of the Taiping Coal Mine in south-western Shandong province, the bottom aquifer of the Quaternary system is moderate to excellent in water-yielding capacity.The base rock above the coal seam is very thin in the concealed coal field of the Carboniferous and Permian systems.Therefore, a comprehensive dewatering plan from both the ground surface and the panel was proposed to lower the groundwater level in order to ensure mining safety.According to the hydrogeologic conditions of the 8th District, we established a numerical model so that we could simulate the groundwater flow in the dewatering process.We obtained the simulation parameters from previous data using backward modeling, such as the average coefficient of permeability of 12 m/d and the elastic storage coefficient of 0.002.From the same model, we predicted the movement of groundwater and water level variables and obtained the visible effect of the dewatering project.Despite the overburden failure during mining, no water and/or sand inrush occurred because the groundwater level in the bottom aquifer was lowered to a safe water level.
文摘Among all fruit crops of horticultural importance, grapevines (Vitis vinifera L.) stand out as the most drought tolerant crop species whose tolerance is credited to their proficiency to recover from water stress in both the natural and vineyard growing conditions. However, information on the recovery responses is relatively scant. Studies were conducted to address this issue using potted vines of the grapevine cultivar, Cabernet Sauvignon, which was subjected to water stress and along with anatomical and ultrastructural characterizations, physiological status was assessed in healthy and water stressed vines, and following recovery via rewatering from the water stressed vines. Water stress induced wilting of leaves, drooping of tendrils, and desiccation followed by abscission of shoot tip leaving behind a brown scar at the shoot apex. The wilted leaves accumulated ABA, which correspondingly reduced stomatal conductance and leaf water potential. Upon re-watering, both these parameters made a recovery with values similar to healthy leaves. Likewise, leaf anatomical features following rewatering resembled to that of healthy leaves. In clusters, water stress caused shriveling of preveraison (unripened) berries, which regained full turgor following water resupply, whereas the postveraison (ripening) berries in the same cluster remained unaffected as evidenced by the presence of viable mesocarp cells and epicuticular wax in the form of platelets. The study revealed that shoot tip with leaf primordia was most sensitive to water stress followed by fully expanded leaves and preveraison berries, whereas the postveraison berries remained unaffected. This information could be valuable to implementing irrigation strategies towards sustaining grape production in existing vineyards experiencing episodic droughts and targeted areas prone to drought.
基金supported by the grants from the Research Fund of Sichuan Provincial Education Department ( 08ZC011)
文摘[ Objective] To investigate the effects of different water amount on freezing point of raw milk. EMetbodl The freezing point of raw milk added water at different proportions was detected by SWC-LG freezing point detector. The linear regression equation was obtained. The reproduc- ibility was checked. The water content in raw milk was calculated according to the standard regression curve. E Result~ When the water content changed from 0 to 10%, the freezing point of raw milk increased linearly. As evidenced by the reproducibility test, the standard deviation and rela- tive standard deviation were less than 1%. The milk concentration obtained by detecting the freezing point of blind milk samples had an absolute er- ror of no more than 0.5%, compared with actual watering milk concentration. [ ConcluMonl Water content in raw milk can be determined by measur- ing freezing point, and this method is reliable and reproducible.
文摘Water is an important factor affecting growth, yield and distribution of different species. Plant response to water deficit can be in the form of physiological disorders, such as reduction in transpiration or assimilating partitioning to root growth. Sustainable use of water has become a priority in agriculture and thus innovative irrigation management practices are critical. The study aimed at investigating how watering frequency and terminal water stress influence growth of Pelargonium sidoides, an important medicinal plant in Southern Africa. The trial was a randomized complete block design with three replicates, and treatment factors were watering frequency (everyday, twice and once a week) and terminal water stress (no watering four weeks before harvesting, no watering two weeks before harvesting and no terminal stress). There was an interacting effect of watering frequency and terminal water stress on biomass and fresh root yield. More frequent watering resulted in significantly higher biomass and fresh root yield, compared to other treatments. Watering everyday with terminal or no terminal water stress resulted in higher fresh root yield, compared to other watering treatments with terminal water stress. Plant height and leaf area were significantly affected by watering frequency and terminal water stress, respectively. A significant drop in stomatal conductance of plants watered everyday was observed 240 d after treatment implementation, such that there was no significant difference across all the three watering frequency treatments. In conclusion, farmers can save on irrigation costs by reducing watering frequency, as there was no significant difference on dry root yield.
基金supported by the National Natural Science Foundation of China(Nos.51478041,51678053)Major Projects on Control and Rectification of Water Body Pollution(Nos.2012ZX07105-002-03,2013ZX07202-010)
文摘In this study, a magnetic micro-particle conditioning–pressurized vertical electro-osmotic dewatering(MPEOD) process with magnetic micro-particle conditioning–drainage under gravity–mechanical compression–electrical compression(MMPC–DG–MC–EC) stages was established to study the distribution and migration of water, extracellular polymeric substances(EPS), and other organic matter in the activated sludge(AS) matrix at each stage.Results showed that the MPEOD process could attain 53.52% water content(WC) in dewatered AS with bound water(BW) and free water(FW) reduction rates of 82.97% and 99.67%,respectively. The coagulation and time-delayed magnetic field effects of magnetic microparticles(MMPs) along the MMPC–DG–MC stages initiated the transformation of partial BW to FW in AS. EC had a coupling driving effect of electro-osmosis and pressure on BW, and the changes in pH and temperature at EC stage induced the aggregation of AS flocs and the release of partial BW. Additionally, MMPs dosing further improved the dewatering performance of AS by acting as skeleton builders to provide water passages. Meanwhile, MMPs could disintegrate sludge cells and EPS fractions, thereby reducing tryptophan-like protein and byproduct-like material concentrations in LB-EPS as well as protein/polysaccharide ratio in AS matrix, which could improve AS filterability. At EC stage, the former four Ex/Em regions of fluorescence regional integration analysis for EPS were obviously reduced, especially the protein-like substances in LB-and TB-EPS, which contributed to improvement of AS dewaterability.
基金This research was funded by the Innovative Research Funding of the Science and Technology Commission of Shanghai Municipality(Grant No.18DZ1201102).This financial support is greatly appreciated.
文摘This paper presents a case study on groundwater control and environmental protection during a deep excavation of the foundation pit for the Liyang Road Station of Metro Line 10 in Shanghai.A three-dimensional finite element simulation model was constructed to quantify the effects of a dewatering process on the environment around the excavation pit.To understand the decrease in the groundwater level around the foundation pit,the ground settlement and groundwater level were studied.During the excavation,environmental protection methods were used in the design of the optimal watering system to check its detrimental environmental effects.The effects of the diaphragm wall and horizontal barrier system were analyzed by varying their insertion depths and the resulting changes in the ground settlement and groundwater level were studied.It was found that increasing the insertion depth of the diaphragm wall reduced settlement near the excavation site but increased the construction costs.The diaphragm wall used in combination with a horizontal barrier was found to be effective in arresting the decrease in groundwater level and reducing settlement around the excavation site.
基金Project supported by the National Natural Science Foundation of China (No. 50879023)the National Hi-Tech Research and Development Program (863) of China (No. 2007AA11Z135)the Min-istry of Water Resources Nonprofit Public Industry Special Foundation of China (No. 200701045)
文摘Due to large-scale dredging operations, a large amount of sludge is inevitably produced. Large areas of land are occupied when the dredged sludge is discarded in the disposal site as waste material. The sludge dewatering with aeration-vacuum (SDAV) method is suit for treating the sludge with high water content and high clay content in the disposal site. The water in the sludge can be discharged out. The volume of the sludge can be reduced quickly, and the recycling of the land can be accelerated by this method. Most importantly, this technique is an efficient way to deal with clogging problems when pumping water from high water content, high clay content dredged sludge. Vacuum degree range tests, the aeration rate range tests, and the influencing factors of sludge dewatering behavior tests were conducted with a self-developed SDAV model test device. Sludge samples were taken from the South-to-North Water Diversion East Line Project in Huai’an White-Horse Lake disposal site, Jiangsu Province, China. The optimal range of vacuum degree and aeration rate were obtained through the test results, and the mechanisms for how the two factors work and how they affect the sludge dewatering behavior were analyzed. The suitable vacuum degree range in SDAV is below 50 kPa, and the suitable aeration rate is about 1.0 m3/h. The low-vacuum degree contributes to reduce the ad-sorption effect of micro-channels on soil particles in filter material and to maintain the arch structures. Aeration has the effects of expansion, disturbance, changing Reynolds number, and dynamic sieve separating. The pump quantity of water per meter of filter tube (m) has different change rules as the vacuum degree changes under different aeration rates. The reason is that the formed arch structures’ conformation and permeability differ greatly under different combined-conditions of vacuum degree and aeration rate. The optimal combined-condition for dewatering the sludge is 35 kPa with 1.0 m3/h.
基金Funded by the Scientific Research Fund of Wuhan Institute of Technology(No.K2023055)the Key Research and Development Project of Hubei Province(No.2020BCA075)the Shccig-Qinling Program(No.2022QL-XM-ZhuLi-HG-006)。
文摘Cu-Mn co-doped CeO_(2) photocatalyst was successfully synthesized by the sol-gel method to assess its capability in degrading tetracycline.XRD and TEM results showed that Cu and Mn were successfully co-doped into CeO_(2) without forming heterostructure,XPS and photoelectrochemical results revealed that Mn ions doping amplified the generation of photo-induced charge carriers,while Cu ions doping significantly facilitated the interfacial charge transfer process.Notably,the optimized Cu3Mn2CeO_(2) nanoparticles exhibited the highest TC removal efficiency,achieved a rate of 78.18%and maintained a stable cycling performance.
文摘The available test methods for optimal moisture content of cold recycled mixture(CRM)as well as its bulk specific gravity,and theoretical maximum relative density were analyzed in this work.Some test improvements were suggested to improve test control of the CRM road performance based on the discovered flaws.Besides,the properties of reclaimed asphalt pavement(RAP),including the content of old asphalt,penetration index,passing rate of 4.75 mm sieve,and gradation change rate after extraction,were examined.The effects of RAP characteristics on splitting tensile strength,water stability,the high-and low-temperature performance of emulsified asphalt CRM were studied.The results show that the optimum moisture content of CRM should be determined when the compaction work matches the specimen’s molding work.Among the analyzed methods of bulk specific gravity assessment,the dry-surface and CoreLok methods provide more robust and accurate results than the wax-sealing method,while the dry-surface method is the most cost-efficient.The modified theoretical maximum relative density test method is proposed,which can reduce the systematic error of the vacuum test method.The following RAP-CRM trends can be observed.The lower the content of old asphalt and the smaller the change rate of gradation,the smaller the voids and the better the water stability of CRM.The greater the penetration of old asphalt,the higher the fracture work and low-temperature splitting strength.The greater the penetration,the higher the passing rate of 4.75 mm sieve after extraction,and the worse the high-temperature performance of CRM.
基金support from the Czech Science Foundation,project EXPRO,No 19-27454Xsupport by the European Union under the REFRESH—Research Excellence For Region Sustainability and High-tech Industries project number CZ.10.03.01/00/22_003/0000048 via the Operational Programme Just Transition from the Ministry of the Environment of the Czech Republic+1 种基金Horizon Europe project EIC Pathfinder Open 2023,“GlaS-A-Fuels”(No.101130717)supported from ERDF/ESF,project TECHSCALE No.CZ.02.01.01/00/22_008/0004587).
文摘Green hydrogen from water splitting has emerged as a critical energy vector with the potential to spearhead the global transition to a fossil fuel-independent society.The field of catalysis has been revolutionized by single-atom catalysts(SACs),which exhibit unique and intricate interactions between atomically dispersed metal atoms and their supports.Recently,bimetallic SACs(bimSACs)have garnered significant attention for leveraging the synergistic functions of two metal ions coordinated on appropriately designed supports.BimSACs offer an avenue for rich metal–metal and metal–support cooperativity,potentially addressing current limitations of SACs in effectively furnishing transformations which involve synchronous proton–electron exchanges,substrate activation with reversible redox cycles,simultaneous multi-electron transfer,regulation of spin states,tuning of electronic properties,and cyclic transition states with low activation energies.This review aims to encapsulate the growing advancements in bimSACs,with an emphasis on their pivotal role in hydrogen generation via water splitting.We subsequently delve into advanced experimental methodologies for the elaborate characterization of SACs,elucidate their electronic properties,and discuss their local coordination environment.Overall,we present comprehensive discussion on the deployment of bimSACs in both hydrogen evolution reaction and oxygen evolution reaction,the two half-reactions of the water electrolysis process.
基金Funded by Provincial Basic Research Projects(No.LJKMZ20220947)。
文摘The effects of liquid-solid ratio and reaction time on the leaching rate of magnesium at room temperature were investigated,as well as the effects of the molar ratio of MgO/MgCl_(2),the amount of water added,and the amount of acid-impregnated slag dosed on the compressive strength and water resistance of LR-MOC.The results showed that the magnesium element in the boron mud could be maximally leached under the conditions of 1:1 concentration of hydrochloric acid at room temperature,liquid-solid ratio of 2.5 mL·g^(-1),and reaction time of 5 h,and the main products were amorphous SiO_(2) as well as a small amount of magnesium olivine which had not been completely reacted.The LR-MOC prepared using the acid-soaked mixture could reach a softening coefficient of 0.85 for 28 d of water immersion when the molar ratio of MgO/MgCl_(2) was 2.2,the amount of water added was 0 g,and the acid-soaked slag dosing was 40 wt%,which also led to an appreciable late-strength,with an increase of 19.4%in compressive strength at 28 d compared to that at 7 d.Unlike previous studies,LR-MOC prepared in this way has a final strength phase that is not the more easily hydrolysed 3-phase but the lath-like 5-phase.For this phenomenon,we analyzed the mechanism and found that,during the acid leaching process,a part of amorphous SiO_(2) dissolved in the acid leaching solution formed a silica sol,in which Mg^(2+)played a bridging role to make the silica sol more stable.With the addition and hydrolysis of MgO,the silica sol gel coagulation slows down,providing a capping layer to inhibit the hydrolysis of the 5-phase crystals and providing some strength after coagulation.The amorphous SiO_(2) in the other part of the acid-impregnated slag generated M-S-H gel with Mg^(2+)and OH-,which synergised with the dense structure composed of interlocking crystals to improve the water resistance of LR-MOC.
基金support of this work by National Key Research and Development Program of China(2019YFC19059003)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(23KJB430024)+1 种基金Jiangsu Funding Program for Excellent Postdoctoral Talent(2023ZB680)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)are gratefully acknowledged.
文摘The Janus fabrics designed for personal moisture/thermal regulation have garnered significant attention for their potential to enhance human comfort.However,the development of smart and dynamic fabrics capable of managing personal moisture/thermal comfort in response to changing external environments remains a challenge.Herein,a smart cellulose-based Janus fabric was designed to dynamically manage personal moisture/heat.The cotton fabric was grafted with N-isopropylacrylamide to construct a temperature-stimulated transport channel.Subsequently,hydrophobic ethyl cellulose and hydrophilic cellulose nanofiber were sprayed on the bottom and top sides of the fabric to obtain wettability gradient.The fabric exhibits anti-gravity directional liquid transportation from hydrophobic side to hydrophilic side,and can dynamically and continuously control the transportation time in a wide range of 3–66 s as the temperature increases from 10 to 40℃.This smart fabric can quickly dissipate heat at high temperatures,while at low temperatures,it can slow down the heat dissipation rate and prevent the human from becoming too cold.In addition,the fabric has UV shielding and photodynamic antibacterial properties through depositing graphitic carbon nitride nanosheets on the hydrophilic side.This smart fabric offers an innovative approach to maximizing personal comfort in environments with significant temperature variations.
基金the National Natural Science Foundation of China(Nos.41602283,41330633 and 41472250)
文摘In order to prevent the inrushing caused by deep excavations, dewatering measure has to be adopted to decrease the confined water level. In this study, the responses of the strata and supporting system to dewatering in deep excavations are investigated through numerical simulations and case studies. Coupled fluid-mechanical analyses are performed by the use of the numerical software, FLAC3 D. The responses of the ground settlement,base heave and interior columns to the excavation and dewatering are analyzed. Numerical results indicate that the dewatering measure can effectively reduce the uplift of the subsurface soil in the excavation, and decrease the vertical displacement of the supporting system. In addition, field data of two case histories show the similar responses and confirm the validation of the numerical results. Based on the analyses, dewatering in the confined aquifer is recommended as a construction method for controlling the vertical displacement of the strata and supporting system in deep excavations.
基金National Natural Science Foundation of China,Grant/Award Number:82204958 and 82305065Natural Science Foundation of Shandong Province,Grant/Award Number:ZR2020ZD17+2 种基金Traditional Chinese Medicine Emotional Disease and Brain Steady State Regulation Innovation Team,Grant/Award Number:2023KJ191Medical and Health Science and Technology Development Plan Project of Shandong Province,Grant/Award Number:202105010467Traditional Chinese Medicine Science and Technology Project of Shandong Province,Grant/Award Number:Q-2022059。
文摘Background:Previously,a chronic social defeat stress(CSDS)model has been widely-adopted for assessing depressive-like behaviors in animals.However,there is still room for improvement in the CSDS model to safeguard study accuracy and the wel-fare of lab rodents.Our study team developed a novel,standardized apparatus to induce CSDS in rodents and assessed the model's practical adaptability.Methods:An innovative CSDS cage apparatus and water bottle was designed.To evaluate the effectiveness of the newly developed tools,a variety of animal models,including the tail suspension test(TST),sucrose preference test,forced swimming test(FST),novelty-suppressed feeding test,female urine sniffing test,and open field test(OFT),were adopted to assess depressive-like behaviors in mice.Fluoxetine treat-ment was also administered to observe the reversal effect,as part of the validation.Results:The CSDS cage apparatus resulted in the manifestation of depressive-like behaviors in the model mice.Significant reductions in sucrose preference and urine sniffing time were observed,while the OFT revealed decreased central zone total distance,residence time,and frequency of entry.Moreover,increased immobility was found in the FST and TST.Fluoxetine treatment was found to successfully reverse the modeling effect.Conclusion:The CSDS cage apparatus was validated for enhanced usability and ad-dressed the previous challenges of water bottle leakage and lab rodent welfare is-sues.The consistent results from multiple behavioral tests also supported real-world application of the apparatus,offering researchers a promising alternative to conven-tional rodent cages.