Objective:To evaluate the effects of wedelolactone,a major flavonoid from Vietnamese Eclipta prostrata(L)L.,on inflammation and insulin resistance.Methods:Wedelolactone was extracted from the leaves of Vietnamese Ecli...Objective:To evaluate the effects of wedelolactone,a major flavonoid from Vietnamese Eclipta prostrata(L)L.,on inflammation and insulin resistance.Methods:Wedelolactone was extracted from the leaves of Vietnamese Eclipta prostrata(L.)L.with methanol by Soxhlet.The effects of wedelolactone on lipopolysaccharide(LPS)-induced cytokine production,reactive oxygen species(ROS)generation,and nicotinamide adenine dinucleotide phosphate(NADPH)oxidase activities in Raw 264.7 cells were measured by enzyme-linked immunosorbent assay(ELISA),specific immunofluorescent dyes and luminometric analysis,respectively.In addition,its effects on glucose uptake and the protein expression of insulin receptor substrate 1(IRS1)and glucose transporter 4(GLUT4)were examined in 3T3-L1 cells by immunofluorescent dyes and Western blot.Results:Wedelolactone at 30μg/mL significantly inhibited LPS-induced production of tumor necrosis factor-α,interleukin(IL)-6,and IL-8(P<0.01)with no noticeable effects on IL-10 level.It also reduced ROS generation and NADPH oxidase activities in LPS-stimulated Raw 264.7 cells(P<0.01).Furthermore,wedelolactone showed anti-insulin resistance activity,as evidenced by improved glucose uptake and the upregulated expression of IRS1 and GLUT4 in 3T3-L1 cells(P<0.01).Conclusions:Wedelolactone exhibits anti-inflammation and anti-insulin resistance effects,which may be used for the treatment of diabetes and inflammation-associated diseases.展开更多
Medicinal herbs are well known and studied over the past millennia in most of the developing countries as a rational means of treatment against various diseases and disorders.Wedelolactone(WDL),a major bioactive compo...Medicinal herbs are well known and studied over the past millennia in most of the developing countries as a rational means of treatment against various diseases and disorders.Wedelolactone(WDL),a major bioactive compound in Eclipta prostrata L(Eclipta alba L),has been reported with potential benefits in human health against chronic diseases.However,a comprehensive study on WDL pharmacological benefits in various ailments,to the best of our knowledge,is not yet reported.Thereof,the present review provides the recent therapeutic applications in reference to biological and functional activities against major human chronic diseases,including cardiovascular,cancer,diabetes mellitus,liver disease,Alzheimer’s disease,and androgenetic alopecia.In this study,we collected all the relevant experimental information on WDL from Scientific databases such as PubMed,Web of Science,Science Direct,and Google Scholar.Conclusively,WDL is recognized as a key anti-oxidant with both specific regulator and inhibitor of major drug targetable proteins in human chronic diseases and disorders.Hence,WDL as a novel therapeutic bioactive molecule is advised to explore further for relevant pharmacological activities.展开更多
An efficient microwave-assisted extraction tech- nique was used to extract wedelolactone from Eclipta alba. To optimize the effects of the microwave-assisted extraction （MAE） processing parameters on the yield ofwed...An efficient microwave-assisted extraction tech- nique was used to extract wedelolactone from Eclipta alba. To optimize the effects of the microwave-assisted extraction （MAE） processing parameters on the yield ofwedelolactone, a response surface methodology with a central composite rotatable design was employed. Four independent variables were investigated： microwave power, ethanol concentration, extraction time and the solvent-to-solid ratio. The optimum conditions were： microwave power, 208 W; ethanol con- centration, 90%; extraction time, 26.5 min; and solvent-to- solid ratio, 33 mL.g-~. Under the optimal conditions, the extraction yield of wedelolactone was （82.67±0.16）%, which is in close agreement with the value predicted by the statistical model. MAE was also compared to other conventional methods, including ultrasonic assisted extrac- tion, extraction at room temperature and heat reflux extraction. MAE has distinct advantages for the extraction of wedelolactone in terms of both time and efficiency. Therefore, MAE is a reliable method for the extraction of wedelolactone from Eclipta alba.展开更多
基金support from the Fund of The Key Laboratory of Enzyme and Protein Technology,VNU University of Science(KLEPT:22.02).
文摘Objective:To evaluate the effects of wedelolactone,a major flavonoid from Vietnamese Eclipta prostrata(L)L.,on inflammation and insulin resistance.Methods:Wedelolactone was extracted from the leaves of Vietnamese Eclipta prostrata(L.)L.with methanol by Soxhlet.The effects of wedelolactone on lipopolysaccharide(LPS)-induced cytokine production,reactive oxygen species(ROS)generation,and nicotinamide adenine dinucleotide phosphate(NADPH)oxidase activities in Raw 264.7 cells were measured by enzyme-linked immunosorbent assay(ELISA),specific immunofluorescent dyes and luminometric analysis,respectively.In addition,its effects on glucose uptake and the protein expression of insulin receptor substrate 1(IRS1)and glucose transporter 4(GLUT4)were examined in 3T3-L1 cells by immunofluorescent dyes and Western blot.Results:Wedelolactone at 30μg/mL significantly inhibited LPS-induced production of tumor necrosis factor-α,interleukin(IL)-6,and IL-8(P<0.01)with no noticeable effects on IL-10 level.It also reduced ROS generation and NADPH oxidase activities in LPS-stimulated Raw 264.7 cells(P<0.01).Furthermore,wedelolactone showed anti-insulin resistance activity,as evidenced by improved glucose uptake and the upregulated expression of IRS1 and GLUT4 in 3T3-L1 cells(P<0.01).Conclusions:Wedelolactone exhibits anti-inflammation and anti-insulin resistance effects,which may be used for the treatment of diabetes and inflammation-associated diseases.
文摘Medicinal herbs are well known and studied over the past millennia in most of the developing countries as a rational means of treatment against various diseases and disorders.Wedelolactone(WDL),a major bioactive compound in Eclipta prostrata L(Eclipta alba L),has been reported with potential benefits in human health against chronic diseases.However,a comprehensive study on WDL pharmacological benefits in various ailments,to the best of our knowledge,is not yet reported.Thereof,the present review provides the recent therapeutic applications in reference to biological and functional activities against major human chronic diseases,including cardiovascular,cancer,diabetes mellitus,liver disease,Alzheimer’s disease,and androgenetic alopecia.In this study,we collected all the relevant experimental information on WDL from Scientific databases such as PubMed,Web of Science,Science Direct,and Google Scholar.Conclusively,WDL is recognized as a key anti-oxidant with both specific regulator and inhibitor of major drug targetable proteins in human chronic diseases and disorders.Hence,WDL as a novel therapeutic bioactive molecule is advised to explore further for relevant pharmacological activities.
文摘An efficient microwave-assisted extraction tech- nique was used to extract wedelolactone from Eclipta alba. To optimize the effects of the microwave-assisted extraction （MAE） processing parameters on the yield ofwedelolactone, a response surface methodology with a central composite rotatable design was employed. Four independent variables were investigated： microwave power, ethanol concentration, extraction time and the solvent-to-solid ratio. The optimum conditions were： microwave power, 208 W; ethanol con- centration, 90%; extraction time, 26.5 min; and solvent-to- solid ratio, 33 mL.g-~. Under the optimal conditions, the extraction yield of wedelolactone was （82.67±0.16）%, which is in close agreement with the value predicted by the statistical model. MAE was also compared to other conventional methods, including ultrasonic assisted extrac- tion, extraction at room temperature and heat reflux extraction. MAE has distinct advantages for the extraction of wedelolactone in terms of both time and efficiency. Therefore, MAE is a reliable method for the extraction of wedelolactone from Eclipta alba.
