Lens fibre differentiation is a life-long process related with lens transparency, and is particularly intense during development, being related with an FGF-2 antero-posterior gradient at the equator level as the main ...Lens fibre differentiation is a life-long process related with lens transparency, and is particularly intense during development, being related with an FGF-2 antero-posterior gradient at the equator level as the main growth factor involved which has been related with the basal membrane of the lens anlagen known as “Lens capsule”. However the lens fibre differentiation induced by FGF2 depends, as in other biological systems, on the local bioavailability of FGF-2 regulated by their relationship with extracellular matrix molecules as Heparan Sulphate Proteoglycans. Here, we try to clarify how Perlecan (a heparan sulphate proteoglycan specific from basement membranes) is involved in lens fibre differentiation at earliest stages of eye development. Our results show that Perlecan, is a major component in the lens capsule during the earliest stages of lens development in chick embryos being present during lens plate induction, lens vesicle stage and the onset of lens fibre differentiation. In order to demonstrate a direct involvement of HSPG-Perlecan in lens fibre differentiation, we generate depleted lenses by HSPG-Perlecan synthesis disruption and specific enzymatic digestion. The HSPG-Perlecan depleted lens show a significant delay or abolition in the lens fibre differentiation which remains in an immature cells displaying DNA synthesis in the posterior epithelium and a decrease in FGF2 lens expression. These data support the hypothesis that lens capsule HSPG-Perlecan is a key molecule involved in lens fibre differentiation during development, probably by involvement in FGF-2 biodisponibility.展开更多
In order to verify the properties of the light propagating through a gradient-index （GRIN） fibre probe for optical coherence tomography （OCT）, numerical simulation using the optical software GLAD is carried out. F...In order to verify the properties of the light propagating through a gradient-index （GRIN） fibre probe for optical coherence tomography （OCT）, numerical simulation using the optical software GLAD is carried out. Firstly, the model of the GRIN fibre probe is presented, which is consisted of a single mode fibre （SMF）, a no-core fibre （NCF）, a GRIN fibre lens and an air path. Then, the software GLAD is adopted to numerically investigate how the lengths of the NCF and the GRIN fibre lens influence the performance of the Gaussian beam focusing through the GRIN fibre probe. The simulation results are well consistent with the experimental ones, showing that the GLAD based numerical simulation technique is an intuitive and effective tool for the verification of the properties of the light propagation. In this paper, we find that on the conditions of a constant GRIN fibre lens length of 0.1 mm and an NCF length of 0.36 mm, the working distance of the probe will be 0.75 mm and the focus spot size is 32 μm.展开更多
文摘Lens fibre differentiation is a life-long process related with lens transparency, and is particularly intense during development, being related with an FGF-2 antero-posterior gradient at the equator level as the main growth factor involved which has been related with the basal membrane of the lens anlagen known as “Lens capsule”. However the lens fibre differentiation induced by FGF2 depends, as in other biological systems, on the local bioavailability of FGF-2 regulated by their relationship with extracellular matrix molecules as Heparan Sulphate Proteoglycans. Here, we try to clarify how Perlecan (a heparan sulphate proteoglycan specific from basement membranes) is involved in lens fibre differentiation at earliest stages of eye development. Our results show that Perlecan, is a major component in the lens capsule during the earliest stages of lens development in chick embryos being present during lens plate induction, lens vesicle stage and the onset of lens fibre differentiation. In order to demonstrate a direct involvement of HSPG-Perlecan in lens fibre differentiation, we generate depleted lenses by HSPG-Perlecan synthesis disruption and specific enzymatic digestion. The HSPG-Perlecan depleted lens show a significant delay or abolition in the lens fibre differentiation which remains in an immature cells displaying DNA synthesis in the posterior epithelium and a decrease in FGF2 lens expression. These data support the hypothesis that lens capsule HSPG-Perlecan is a key molecule involved in lens fibre differentiation during development, probably by involvement in FGF-2 biodisponibility.
基金supported by the International Cooperation Project of Shanghai Science and Technology Committee,China(Grant No.09530708700)
文摘In order to verify the properties of the light propagating through a gradient-index （GRIN） fibre probe for optical coherence tomography （OCT）, numerical simulation using the optical software GLAD is carried out. Firstly, the model of the GRIN fibre probe is presented, which is consisted of a single mode fibre （SMF）, a no-core fibre （NCF）, a GRIN fibre lens and an air path. Then, the software GLAD is adopted to numerically investigate how the lengths of the NCF and the GRIN fibre lens influence the performance of the Gaussian beam focusing through the GRIN fibre probe. The simulation results are well consistent with the experimental ones, showing that the GLAD based numerical simulation technique is an intuitive and effective tool for the verification of the properties of the light propagation. In this paper, we find that on the conditions of a constant GRIN fibre lens length of 0.1 mm and an NCF length of 0.36 mm, the working distance of the probe will be 0.75 mm and the focus spot size is 32 μm.
