Objective： To evaluate the effects of the ethanol extract isolated from Weiqi Decoction （胃祺饮, WQD-EE） on AGS cell proliferation and apoptosis. Methods： By using high-performance liquid chromatography with ultra...Objective： To evaluate the effects of the ethanol extract isolated from Weiqi Decoction （胃祺饮, WQD-EE） on AGS cell proliferation and apoptosis. Methods： By using high-performance liquid chromatography with ultraviolet detectors （HPLC-UV） assay and MTT method, the main compounds in WQD-EE and cell viability were detected. And cell cycle distributions were determined by flow cytometry with propidium iodine （PI） staining while apoptosis was detected by flow cytometry with annexin V/PI double staining. Finally, caspase-3 activities were measured by colorimetric method and protein expression was determined by Western blotting. Results： HPLC analysis showed that naringin （35.92 μg/mg）, nobiletin （21.98 μ g/mg）, neohesperidin （17.98μg/mg） and tangeretin （0.756μ g/mg） may be the main compounds in WQD-EE. WQD-EE not only inhibited AGS and MCF 7 cell proliferation in a dose-dependent manner, but also blocked cell cycle progression at G2/M stage as well as inducing cell apoptosis at concentrations triggering significant inhibition of proliferation and cell cycle arrest in AGS cells. While at 0.5 mg/mL, WQD-EE significantly increased caspase-3 activity by 2.75 and 7.47 times at 24 h and 48 h, respectively. Moreover, WQD-EE in one hand reduced protein expressions of p53 and cyclin B1, and in other hand enhanced protein expressions of cytochrome c and Bax. Protein levels of Bcl-2, Fas L and Fas were not significantly affected by WQD-EE. Conclusions： WQD-EE inhibits AGS cell proliferation through G2/M arrest due to down-regulation of cyclin B1 protein expression, and promotes apoptosis by caspase-3 and mitochondria-dependent pathways, but not by p53-dependent pathway.展开更多
AN elegant, poised woman sits in front of a weiqi board, lightly placing a piece on the board with her delicate fingers. This is one piece of the series Playing Weiqi (Tang Dynasty, 618-907 A.D.). Weiqi is an importan...AN elegant, poised woman sits in front of a weiqi board, lightly placing a piece on the board with her delicate fingers. This is one piece of the series Playing Weiqi (Tang Dynasty, 618-907 A.D.). Weiqi is an important part of the traditional Chinese culture. They say it has a history展开更多
Weiqi is an important class in the third primary school of Tiantai Town. Shown here is a weiqi class of third grade pupils. Through special training and in their spare time, children could reach the third to fifth gra...Weiqi is an important class in the third primary school of Tiantai Town. Shown here is a weiqi class of third grade pupils. Through special training and in their spare time, children could reach the third to fifth grades in weiqi.展开更多
TANGQIAO is a township of Zhangjiagang City, Jiangsu Province. Ordinary in other ways, this town is unusual for the number of weiqi (go) players it boasts—8,000 of its population of 28,000. Almost every family has mo...TANGQIAO is a township of Zhangjiagang City, Jiangsu Province. Ordinary in other ways, this town is unusual for the number of weiqi (go) players it boasts—8,000 of its population of 28,000. Almost every family has more than one weiqi enthusiast. In recent years, the township’s players have earned themselves uncountable championships and runner-up placements at provincial-and national-level weiqi competitions. Thus, the State Physical Culture and Sports Commission awarded Zhangjiagang City the title "National Home of Weiqi" in July, 1990. In May of 1991, the Jiangsu Physical Culture展开更多
基金Supported by High Level Project of University of Educational Commission of Shanghai,Shanghai,China(No.2008GSP19)Shanghai Municipal Natural Science Foundation,Shanghai China(09ZR1431800)+1 种基金Opening Project of Shanghai Key Laboratory of Complex Prescription,Shanghai,China(No.11DZ2272300)Educational Commission of Shanghai,Shanghai,China(No.09JW21 and No.2012JW19)
文摘Objective： To evaluate the effects of the ethanol extract isolated from Weiqi Decoction （胃祺饮, WQD-EE） on AGS cell proliferation and apoptosis. Methods： By using high-performance liquid chromatography with ultraviolet detectors （HPLC-UV） assay and MTT method, the main compounds in WQD-EE and cell viability were detected. And cell cycle distributions were determined by flow cytometry with propidium iodine （PI） staining while apoptosis was detected by flow cytometry with annexin V/PI double staining. Finally, caspase-3 activities were measured by colorimetric method and protein expression was determined by Western blotting. Results： HPLC analysis showed that naringin （35.92 μg/mg）, nobiletin （21.98 μ g/mg）, neohesperidin （17.98μg/mg） and tangeretin （0.756μ g/mg） may be the main compounds in WQD-EE. WQD-EE not only inhibited AGS and MCF 7 cell proliferation in a dose-dependent manner, but also blocked cell cycle progression at G2/M stage as well as inducing cell apoptosis at concentrations triggering significant inhibition of proliferation and cell cycle arrest in AGS cells. While at 0.5 mg/mL, WQD-EE significantly increased caspase-3 activity by 2.75 and 7.47 times at 24 h and 48 h, respectively. Moreover, WQD-EE in one hand reduced protein expressions of p53 and cyclin B1, and in other hand enhanced protein expressions of cytochrome c and Bax. Protein levels of Bcl-2, Fas L and Fas were not significantly affected by WQD-EE. Conclusions： WQD-EE inhibits AGS cell proliferation through G2/M arrest due to down-regulation of cyclin B1 protein expression, and promotes apoptosis by caspase-3 and mitochondria-dependent pathways, but not by p53-dependent pathway.
文摘AN elegant, poised woman sits in front of a weiqi board, lightly placing a piece on the board with her delicate fingers. This is one piece of the series Playing Weiqi (Tang Dynasty, 618-907 A.D.). Weiqi is an important part of the traditional Chinese culture. They say it has a history
文摘Weiqi is an important class in the third primary school of Tiantai Town. Shown here is a weiqi class of third grade pupils. Through special training and in their spare time, children could reach the third to fifth grades in weiqi.
文摘TANGQIAO is a township of Zhangjiagang City, Jiangsu Province. Ordinary in other ways, this town is unusual for the number of weiqi (go) players it boasts—8,000 of its population of 28,000. Almost every family has more than one weiqi enthusiast. In recent years, the township’s players have earned themselves uncountable championships and runner-up placements at provincial-and national-level weiqi competitions. Thus, the State Physical Culture and Sports Commission awarded Zhangjiagang City the title "National Home of Weiqi" in July, 1990. In May of 1991, the Jiangsu Physical Culture
