Acid-Phosphorus Activity of Wheat Varieties Rhizobacteria of Uzbekistan

In this work, local strains of phosphate-solubilizing microorganisms were isolated and identified from the wheat rhizosphere and exogenous acid phosphatase enzymes of locally active phosphate- and potassium-mobilizing rhizobacteria belonging to the genera Escherichia, Rahnella, Bacillus, Enterobacter, Pseudomonas, and Pantoea were studied. The efficiency of the physiological properties of rhizobacteria is determined by the production of soluble phosphorus, and the amount of phosphorus depends on the activity and biomass of bacteria that secrete phosphorus. This is done by phosphate solubilizing bacteria, and the habitat ecosystem is enriched with beneficial micronutrients. In these studies, active rhizobacteria activity of acid phosphatase in nutrient liquid was studied at different temperatures. Optimum pH activity index and temperature variability of enzymes were determined. It should be noted that in the most active phosphate-solubilizing strains the maximum enzymatic activity was observed in the culture fluid of R. aquatilis strain 17, which produced 1.086 μmol p-nitrophenol μmol/min/ml. P. agglomerans 22, P. agglomerans 20 and Ps. kilonensis 32 cultures phosphatase activity was 0.143 - 0.680 p-nitrophenol μmol/min/ml. It should be noted that the phosphatase activity of bacteria belonging to the same genus and species was very different from each other. That is, the enzyme activity of Rahnella aquatilis strain 17 was 9 times higher than the enzyme activity of Rahnella aquatilis strain 9. The pH optimum of sour phosphatase enzymes in Rahnella aquatilis strain 16 was 6.0. The optimum temperature of acid phosphatase activity was 45˚C and 50˚C. The reason for this may be that the strains were isolated in different soil and climate conditions. When the acid phosphatase activity of R. aquatilis 3, 9, E. cloacae 8 and P. agglomerans 22 cultures was determined at a temperature of 45˚C, it was observed that the enzyme activity increased by 2 - 4 times. Es. hermannii 1, Ps. kilonensis 26 and B. simplex 28 bacteria acid phosphatase activity was not significantly affected by temperature rise.

Analysis on Grain Traits and Sedimentation Value of Wheat Genotype Heng 9966, Its Parents and Their Closely Related Varieties

[Objective] 7 indictors of wheat genotype Heng 9966, its parents and their closely related varieties were analyzed based on the 1000-grain weight, sedimentation value and traits of selected grains in DUS test, so as to provide references for the protection of variety intellectual property. [Method] Testing comparison and statistical analysis were conducted to the dormant seeds of winter wheat genotype 9966, parents of Liangxing 99 and Liangxing 96 aw well as the closely related variety of the parents, Jimai 22. [Result] Heng 9966 showed a difference of 2 codes in 1000-grain weight and sedimentation value from the above-mentioned 3 genotypes, and the variant analysis reached the extremely significant level of 0.01. And it also showed extremely significant difference at the level of 0.01 with Liangxing 66 in grain length, with Liangxing 66 and Jimai 22 in grain width, with Liangxing 66 in grain area, with Liangxing 66 and Jimai 22 in grain perimeter. [Conclusion] Heng 9966 had significant distinctness from its parents and their closely related variety, and grain shape can be used as a reference indicator for variety distinctness for the protection of variety intellectual property.

Analysis on Marginal Superiority and Yield of Different Wheat Varieties in Wheat-cotton Intercropping System

In wheat-cotton intercropping system,the spike number per unit area,grains per spike,thousand-grain weight and yield of side line and inner line were investigated in six wheat varieties to study the marginal superiority and select suitable wheat varieties intercropped with cotton.The results showed that Xingmai No.4had obvious marginal superiority with the yield of 6 919.0 kg/hm2,so it was the suitable variety for wheat-cotton intercropping system.

An Analysis on Utilization of Wheat Varieties in Henan Province

The new wheat varieties with more than 6.67x104 hm2 of planting area in Henan Province during 2007-2017 were analyzed. The results showed that in the past eleven years, there were total 43 varieties from 23 breeding units of which the annual planting areas were more than 6.67x104 hm2. Among them, the annual planting areas of 13 varieties from 7 breeding units exceeded 3.33x10 hm2. The total cultivation area of 43 wheat varieties accounted for about 80% of the total wheat cultivation area in Henan in that year. Most of the varieties come from provincial universities and research institutes and municipal agricultural academies, Zhengmai 9023 was the spring wheat variety that had the largest annual and accu- mulated cultivation area, and Aikang 58 was the semi-winter wheat cultivar with the largest annual and accumulated planting area. Semi-winter varieties were the domi- nator. Among the wheat varieties planted in large area in Henan Province, medium- gluten and state-approved varieties are dominant; and provincial and municipal academies are the breeders of the major wheat cultivars in Henan.

The Red Edge Parameters of Different Wheat Varieties Under Different Fertilization and Irrigation Treatments

The dynamics of red edge parameters at different growth stages of wheat canopy was studied. The red edge position moved to the longer wavelength from erecting stage to heading stage, and returned to the original wavelength direction from grain-filling stage to maturity stage. The leaf total nitrogen content (LTN) is positively related to red edge amplitude (d λ red) and NIR platform amplitude (d λNIRP) and the correlation coefficient improved as growth stage extended. The chlorophyll content (TChl) is negatively related to d λ red from erecting stage to the elongation stage, and positively related to d λ red from heading stage to milky maturity stage. TChl is also positively related to d λNIRP. The leaf area index (LAI) is positively related to d λ red and d λNIRP, and the correlation coefficient improved as growth stage extended. It was concluded that d λ red and d λNIRP can be used as the indicators of winter wheat growth state, and guide the fertilization and irrigation decision making. The λNIRF is suitable for derivation of LTN, the d λ red for TChl, and the d λ red or d λNIRP for LAI.

Allelic Variation of Glu-A1, Glu-B1 and Glu-Dl in Chinese Released Wheat Varieties in the Last 50 Years

: The high-molecular-weight glutenin subunit (HMW-GS) components from about 85 varieties were fractionated by SDS-PAGE, including 22 corner stone breeding parents, 45 varieties taking important role in Chinese wheat production and 18 varieties with good bread making quality. The 22 corner stone breeding lines carry either null (c) or l(a) summits on Glu-Al loci. Five types are detected on Glu-Bl, which are 7 + 8(b), 7 + 9 (c), 14 + 15 (h), 17 + 18 (i), 6 + 8 (d). 7 + 8 and 7 + 9 are the two major types. Six types have been detected on Glu-Dl, which are 2 + 12 (a), 2 + 10 (e), 5 + 10 (d), 4 + 10 (j), 4 + 12 (c), 2 + 11 (g). 2 + 12 and 2 + 11 are the two major types. In the corner stone breeding lines, early premium from American carries 5 + 10, St2422/464 from Italy carries the 14 + 15. In addition, two relatively essential varieties, Mara and Alondra carry 5 + 10. In the 45 commercialized varieties, sowed more than 666 000 ha (10 million mu) annually, only Yangmai 5 conveys the 5 + 10 on Glu-Dl, and Xiaoyan 6 and Yumai 7 carry the 14 + 15 on Glu-B1. Four varieties, Funo, Nongda 139, Zhengzhou 683 and Fan 6 convey the 17 + 18 on Glu-B1. The 18 bread wheat varieties recommended by the Ministry of Agriculture in 1992 could be classified into two groups, 5 + 10 and 14 + 15. Zhongzuo 8131 and its selections are the typical genotype of 5 + 10. Genes coding the two subunits are from either Yecora F-70 or IRN68-181. Xiaoyan 6 and its derivative varieties are the typical genotype of 14 + 15. The genes coding 17 + 18 subunits in Chinese varieties were derived from Funo or its selections. These results basically reflect allelic changes on Glu-1 in Chinese wheat varieties in the past 50 years. It is also proven that HMW-GS components may have great diversity between lines from the same cross, which causes great difference on baking quality.

Distribution and Selective Effects of Vrn-A1,Vrn-B1,and Vrn-D1 Genes in Derivative Varieties from Four Cornerstone Breeding Parents of Wheat in China

Vernalization, the process of a long exposure to low temperature to induce flowering in plants, is essential for plants to adapt to cold winters. The presence of vernalization genes Vrn-A1, -B1, and -D1 in four cornerstone breeding parents of wheat in China （Funo, Mentana, Yanda 1817, and Bima 4） and 322 derivative varieties （mostly winter wheat） from these parents were determined using PCR based molecular method. The frequencies of the VRN-1 genes in these derivative varieties were in order of Vrn-Dl（67.1%）〉 Vrn-Bl（19.6%）〉 Vrn-Al（5.3%）, which are similar as the former conclusion that Vrn-D1 is associated with the latest heading time, Vrn-A1 the earliest, and Vrn-B1 intermediate values. The frequencies of Vrn-Al and Vrn-B1 loci in the derivative varieties from winter wheat zones were higher than that from spring winter zones. Based on the wheat breeding history in China and the fact of non-random distribution of Vrn-A1 and Vrn-B1 in the derivative varieties from the four parents, there could be a strong selective effect on VRN-1 genes in different regions where the derivative varieties were cultivated.

Spatial distribution of Pb and its correlation at different grain positions among wheat varieties for specific end-uses

The relative content of Pb and its correlation were investigated in endosperm, aleurone layer and pericarp respectively among different wheat varieties for specific end-uses (WVFSE) using scanning electron microscope (SEM) equipped with the energy dispersive X-ray spectroscope (EDS). The results showed that Pb contents in grains at different positions followed the order of endosperm > pericarp > aleurone. The differences of Pb contents varied among the WVFSE, and wheat with strong gluten had a highest average content of Pb, while wheat with medium gluten had a lowest one. There were significant third order equation correlations between Pb content in endosperm and that in aleurone layer and that in pericarp, respectively. And good correlation coefficients were obtained. However, the correlation differed at different position among WVFSE, which indicated that Pb contents in endosperm, aleurone layer and pericarp were regulated by each other.

Agro-Morphology Evaluation and Gluten Content Estimation of Wheat (Triticum aestivum L.) in the Western Highland of Cameroon

Agriculture is amongst the major occupations in Cameroon where over 70% of citizens are involved and it contributes enormously to the economy of the country. Wheat is one of the most consumed cereals in Cameroon with very high importation rate. However, the adoption of wheat production in the cropping system could have the potential to pull farmers out of poverty. It is essential in human foods and animal feeds. This study aims to investigate on the adaptability of wheat varieties based on growth traits and yield as well as to estimate the gluten content in each of the tested variety in the North-West region. Eight wheat varieties (five from CIMMYT, two from IRAD and one local variety) were evaluated in a factorial design with two types of fertilization (organic and inorganic), in two site (Santa and UBa farm) and five environments. Agro-morphological data were collected and were subjected to the analysis of variance using R software. The gluten content related to the baking quality of wheat flour was estimated per tested variety. Highly significant differences were observed among varieties, sites, environment and fertilization for all parameters estimated. The general mean of all the traits evaluated was significantly higher when using organic fertilizer than inorganic, meaning that the application of organic fertilizer provides better performance of wheat growth. The elevated number of tillers found in Santa could inform on the high level of soil fertility for wheat production in that area. Environment 1 was found to be the best follow by environment 3 and 5. IRAD I gave the highest yield followed by Alexander Wonder and IRAD II. 11SATYND and 29SAWYT were promising introduced varieties in term of grain weight when using organic fertilization. Wet and dry gluten yield varied from 3.8 (ALEXANDER Wonder) to 5.5 (IRAD I) and from 3.7 (IRAD II) to 7.9 (IRAD I) respectively. All the introduced wheat varieties expressed low wet and dry gluten yield as compare to the check Amigo. IRAD I was the best variety to be produced for industrial purposes taken into account the high level of gluten content. IRAD I, 42ESWYTB and IRAD II were found to have their moisture content percentage of flour below that of the check (Amigo) and therefore could be recommended for manufactured foods.

Science Letters:Nutritional composition of Pakistani wheat varieties

Pakistani wheat varieties are grown over a wide agro-climatic range and as such are anticipated to exhibit yield and quality differences. It is therefore necessary to investigate the nutritional status of wheat varieties in terms of biochemical and physiochemical characteristics available for food and nutritional purposes in Pakistan. The result shows that wheat grains of different varieties contain a net protein level of 9.15%～10.27%, 2.15%～2.55% total fats, 1.72%～1.85% dietary fibers, 77.65×10-6～84.25×10-6 of potassium and 7.70×10-6～35.90×10-6 of sodium ions concentration, 0.24×10-6～0.84×10-6 of phosphorus, 1.44%～2.10% ash, 31.108～43.602 g of thousand grain mass (TGM) and 8.38%～9.67% moisture contents. This study is significant in providing an opportunity to explore the available wheat varieties and to further improve their nutritional excellence and also essential for setting nutritional regulations for domestic and export purposes.

Study on Selection of Wheat Varieties for Noodle Production in Hebei Province and Factors Affecting the Processing Quality

[Objectives]To screen out the specific wheat varieties for noodle production in Hebei Province and analyze the factors affecting the processing quality of noodle.[Methods]The quality characteristics of grains,flour and noodle of major medium gluten wheat varieties in Hebei Province were detected and analyzed.[Results]The sensory score of noodle is significantly positively correlated with wet gluten content of medium gluten wheat flour,and extensile length,cohesiveness,resilience,elasticity,gumminess,chewiness and extensile distance of TPA of noodle,and is significantly negatively correlated with softening degree.TPA of noodle can indirectly reflect sensory quality of noodle.[Conclusions]Han 7086,Xingmai 4,Liangxing 66,Shiyou 17,Heng 5229 and Liangxing 99 are suitable for producing high-quality noodle,and they can be used as noodle-specific varieties for promoting in Hebei Province.

Identification of Resistance of Powdery Mildew and Stripe Rust of Winter Wheat Strains in Xinjiang

Wheat powdery mildew and stripe rust are the major diseases in wheat producing area in Xinjiang.To obtain wheat germplasm resources and varieties resistant to powdery mildew and rust,36 high-generation stable strains of Xinjiang winter wheat were evaluated using the method of natural inducement from 2018 to 2020.A total of 5 strains with high resistance to powdery mildew,4 strains with slow stripe rust and 1 strain with resistance to powdery mildew and adult plant slow stripe rust were obtained.And the parental combination of disease-resistant varieties was analyzed.These studies will provide theoretical basis for the breeding of resistant wheat varieties in Xinjiang.

Durum Wheat Bread： Old Sicilian Varieties and Improved Ones

In Mediterranean countries and particularly in Southern Italy, durum wheat （Triticum durum Desf.） is traditionally used for bread production. Market globalization has created, also in these regions, the spread of bread made with soft wheat flour （Triticum aestivum L.） standardizing this product flattening consumers＇ taste. With the aim of re-evaluating the old Sicilian local varieties, some landraces, chosen from the genebank of the institute, have been multiplied on farmers＇ fields to have enough material for making bread and for comparing this product with bread made by improved new varieties. Before milling in the pilot plant, the different wheats were characterized for their merceological indexes. The semolina obtained was analyzed for its chemical parameters. Rheological tests were conducted on dough and, finally, using standardized methods, one-variety breads were produced. On the bread slices, computerized image analysis was performed to measure colour and morphological parameters of crumb and crust. Sensory analysis, achieved through a descriptive profiling test, was conducted with trained judges using touch, fragrance and taste attributes for the different bread types. The different merceological, chemical and theological properties of raw material, grain and semolina were reflected in the breads prepared using old or improved varieties. Finally, the data obtained pointed out that the bread prepared with old and improved varieties can be clearly distinguished both by objective morphological parameters （image analysis） and by sensorial evaluations, for a potential traceability of typical product.

高产抗条锈冬小麦新品种天选79号选育报告

为选育适宜甘肃陇南麦区及同类地区推广种植的抗病、丰产且适应性广的冬小麦品种,天水市农业科学研究所以自育高代品系00127-2-2-3作母本、周麦22号作父本配制杂交组合,经多年多点连续鉴定选择,培育成了抗条锈高产冬小麦新品种天选79号。2019—2021年参加甘肃省陇南片川区组区域试验,2 a 10点(次)平均折合产量7437.90 kg/hm^(2),较对照品种兰天33号增产6.87%。2021—2022年度参加生产试验,5点(次)平均折合产量6910.35 kg/hm^(2),较对照品种兰天33号增产3.25%。该品种株高78.4 cm,穗长8.0 cm,每穗粒数40.96粒,籽粒白色,硬质,千粒重46.31 g,有效穗数570万穗/hm^(2)。具有抗条锈、稳产、抗倒伏、籽粒品质优良等特点,适宜在甘肃省天水渭河川道区及陇南麦区低海拔区种植。

混合卷积神经网络用于高光谱小麦品种鉴别

不同品种的小麦满足了市场的不同需求,同时也会带来小麦品种混杂的风险。为了提高小麦品种的纯度进而提高选种、育种、加工等环节的经济价值,小麦种子的鉴别起到关键作用。传统的小麦品种纯度理化分析鉴别方法,鉴定时间长且破坏种子,已不能满足现代农业的迫切需要。高光谱成像作为近年来发展迅速的一种快速、高效、无损的新型鉴别技术,在种子品种鉴别领域取得了显著成效。然而,已有的大多数高光谱分类方法仅利用光谱信息,没有充分考虑空间信息,分类效果较差。为了解决上述问题,利用高光谱成像设备采集8个品种的小麦种子正背面的高光谱图像,基于这些高光谱数据集,提出一种基于注意力机制的混合卷积神经网络的高光谱小麦品种鉴别方法,主要利用三维卷积和二维卷积的互补优势特性提取小麦的有价值特征,进而提高小麦品种的鉴别效果。具体而言,首先提取小麦品种的感兴趣区域,并利用多元散射校正方法削弱由于散射水平差异带来的同一品种的光谱差异。同时,利用主成分分析方法减少三维数据的无用光谱波段,进而保留并压缩对鉴别小麦品种有价值的特征。随后,利用三维卷积获取空间维度和不同光谱间的特征信息,二维卷积获取空间信息和图像的自身固有的特征信息,并在二维卷积模型中引入注意力机制进一步增强图像的特征信息的提取。最后在全连接层实现同一区域不同小麦品种的鉴别。实验表明,所提出的方法比其他方法具有较好的分类性能,分类准确率达97.92%。此外,所提出的方法对小样本数据也具有较好的分类性能。总的来说,提出的方法对于高光谱小麦种子鉴别具有较好的有效性和鲁棒性,为小麦种子的在线鉴别提供了一种新的方法。

小麦新品系赤霉病抗性鉴定及基因效应分析

为创制黄淮麦区小麦新品系、提高小麦抗赤霉病育种效率,2022年在小麦扬花期利用喷洒孢子液的方法对81份抗赤霉病高代品系和4份对照品种在濉溪柳湖试验基地开展了赤霉病抗性鉴定,并利用与4个抗赤霉病主效基因Fhb1、Fhb2、Fhb4和Fhb5紧密连锁的6个标记对供试小麦材料进行了基因型检测。结果表明,供试81份材料的赤霉病抗性整体较好,平均病小穗率为27.95%,其中13份自育品系平均病小穗率低于扬麦20;携带单个或多个赤霉病抗性基因的品系共有60份,Fhb1、Fhb2、Fhb4、Fhb5检出频率分别为64.20%、12.35%、20.99%、34.57%;携带单个抗病基因Fhb1、Fhb4、Fhb5的小麦品系分别为18份、3份、2份;有45.68%品系同时携带2~3个赤霉病抗性基因。携带抗性基因的品系平均病小穗率低于不含抗性基因的品系;聚合多个抗赤霉病基因可以有效提高小麦品种的抗赤霉病水平。

半干旱地区燕麦种子产量及其相关农艺性状

本研究选取19个燕麦(Avena sativa)品种(系)的产量相关性状指标,采用相关性分析、通径分析、灰色关联度分析以及聚类分析,探讨甘肃中部半干旱地区燕麦种子产量影响因素并筛选综合性状优良的燕麦种质。结果表明:燕麦种子产量为3 283.33~5 833.33 kg·hm^(-2),其中‘Cinskinaly’的产量最高。种子产量与千粒重、成穗数极显著正相关(P <0.01),与单株穗粒重显著正相关(P <0.05),而与株高显著负相关(P <0.05);通径分析结果表明,单株穗粒数、单株穗粒重、千粒重是决定种子产量的重要因素。聚类分析将19份燕麦材料析划分为4个类群:第Ⅰ类可作饲草资源加以筛选利用;第Ⅱ类适合作高产抗倒伏产籽型品种;第Ⅲ类为优选裸燕麦材料;而第Ⅳ类生产适应性和田间表现差,可直接淘汰。筛选出综合表现较佳的两个裸燕麦品系‘201006-01-01-9’‘201003-06-5’和1个皮燕麦品系‘201113-03-05’,可提升到甘肃省区域试验。

土壤水分对不同抗旱性春小麦品种叶片保护性酶活性及产量的影响

【目的】研究土壤水分对不同抗旱性春小麦品种叶片保护性酶活性及产量的影响,为选育春小麦抗旱品种及制定节水高产措施提供理论依据。【方法】在大田条件下,以抗旱性较强的品种新春46号、抗旱性中等的品种新春37号、抗旱性较弱的品种新春26号为材料,设置3种水分处理,研究土壤水分对不同抗旱性春小麦品种旗叶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性、丙二醛(MDA)含量及产量的影响。【结果】随着水分亏缺程度的加剧,春小麦旗叶SOD、CAT活性在扬花期呈升高的趋势,而在灌浆期则呈先升高后降低的趋势;POD活性在扬花期呈先升高后降低的趋势,灌浆期则呈升高的趋势;MDA含量呈升高的趋势;SOD、POD活性表现为抗旱性较强的品种新春46号>抗旱性中等的品种新春37号>抗旱性较弱的品种新春26号,MDA活性在春小麦品种间则呈相反的趋势。收获穗数、穗粒数和产量均随着水分亏缺程度的加剧而降低,抗旱性较强的品种新春46号在有限灌溉和亏缺灌溉处理下,其收获穗数和穗粒数降低幅度小于新春37号和新春26号,且产量高于新春37号和新春26号。【结论】抗旱性较强的品种新春46号在有限灌溉和亏缺灌溉条件下,旗叶SOD、POD酶活性较高,MDA含量较低,活性氧清除能力较强,有效延缓了小麦植株的衰老,收获穗数和穗粒数较抗旱性中等的新春37号和抗旱性较弱的新春26号下降幅度低,在水分亏缺条件下有利于获得较高的产量。

甘肃陇南小麦品种抗条锈病鉴定与评价

为了解甘肃陇南小麦育成品种和后备品种对当前条锈菌流行小种的抗性水平,利用条锈菌流行小种CYR32、CYR33和CYR34对60份供试小麦品种分别进行苗期、成株期抗病性鉴定。结果表明,在所有供试品种中,对CYR32、CYR33和CYR34表现苗期抗病的品种分别有9、22和10份,表现成株期抗病的品种分别有35、38和30份。结合苗期和成株期鉴定结果,对CYR32、CYR33和CYR34表现苗期感病而成株期抗病的品种分别有28、22和24份,占46.7%、36.7%和40.0%;中梁14号和兰天37号对CYR32、CYR33和CYR34均表现全生育期抗性,占3.3%。甘肃陇南麦区小麦品种对当前条锈菌流行小种多表现为成株期抗性,筛选出部分具有全生育期或成株期抗性的品种,可作小麦育种的亲本资源加以利用。

灌溉和雨养条件下不同品种小麦的农艺性状与产量关系研究

论文以百农207、周麦18、洛旱7号3个品种小麦为试验材料,对其在灌溉和雨养两种条件下的产量三因素和其他农艺性状与产量的关系进行研究.结果表明:在灌溉条件下,百农207的经济产量最高,洛旱7号和周麦18次之;在雨养条件下,百农207产量最高,周麦18和洛旱7号次之.产量及其构成因素对雨养胁迫的敏感程度存在着极显著的差异.在产量构成因素与经济产量的相关关系和通径关系中,穗粒数对经济产量的贡献最大;在雨养胁迫环境条件下,3个品种小麦的株高冬前分蘖、最高分蘖、成穗率、生物产量、经济产量和秸秆系数比灌溉条件下均有一定程度的下降,且这些性状的减少均达到了极显著水平.3个品种小麦的收获指数在两种环境条件下的增减均未达到显著水平.综合以上结果可知,百农207对水分条件的敏感程度最低,产量最为突出.