在Pirkle型的 (S ,S) Whelk O 1与纤维素衍生物型的CDMPC两种手性柱上对六种外消旋萘普生酯进行了对映体分离 ,通过研究烷氧基结构上的差异以及流动相中不同的醇类添加剂对手性识别的影响 ,探讨和比较了外消旋萘普生酯在两种手性固定...在Pirkle型的 (S ,S) Whelk O 1与纤维素衍生物型的CDMPC两种手性柱上对六种外消旋萘普生酯进行了对映体分离 ,通过研究烷氧基结构上的差异以及流动相中不同的醇类添加剂对手性识别的影响 ,探讨和比较了外消旋萘普生酯在两种手性固定相上手性识别的机理 .对于 (S ,S) Whelk O 1,溶质与固定相之间的吸引作用是手性识别的主要因素 ,而对于CDMPC 。展开更多
Metamorphosis is the short developmental stage characterized by dramatic ontogenetic changes that occurs in most animals.However,this important process remains largely unclear in marine invertebrates.In this study,we ...Metamorphosis is the short developmental stage characterized by dramatic ontogenetic changes that occurs in most animals.However,this important process remains largely unclear in marine invertebrates.In this study,we performed the sequential RNA sequencing of a representative mollusk,the rapa whelk(Rapana venosa),that is undergoing metamorphosis and conducted differential gene expression analysis and weighted gene co-expression network analysis(WGCNA)to investigate the overall and dynamic transcriptome responses.The results revealed that the expression of cytochrome P4502A and 3A were upregulated during metamorphosis,while the expression of H/ACA ribonucleoproteins increased 4 h after metamorphosis induction(M4 stage),indicating that R.venosa mainly responded to the pelagobenthic changes.At the M24 stage,the enrichment of V-type proton ATPase and insulin indicated the complete development of secretory organs and initiation of hormone secretion.Furthermore,at the M48 stage,the enrichment of zinc metalloproteinase and conotoxin indicated a well-developed predation system that requires exogenous nutrition.Finally,during the PL stage,the genes associated with growth control were highly enriched,implying that R.venosa had completed metamorphosis and has entered the period of rapid growth.Therefore,our study provides useful transcriptomic resources for R.venosa and contributes new insights that may assist in elucidating the mechanisms underlying metamorphosis in marine invertebrates.展开更多
Objective:To determine length-weight relationships,growth type and feeding behavior of the benthic predator Rapa whelk at the coast of Camburnu,south-eastern Black Sea.Methods:Rapa whelk was monthly collected by dredg...Objective:To determine length-weight relationships,growth type and feeding behavior of the benthic predator Rapa whelk at the coast of Camburnu,south-eastern Black Sea.Methods:Rapa whelk was monthly collected by dredge sampling on the south-eastern Black Sea at 20 m depth.The relationships between morphometric parameters of Rapa whelk were described by linear and exponential models.The allometric growth of each variable relative to shell length(SL)was calculated from the function Y=aSL^(b) or logY=loga_(+)blogSL.The functional regression b values were tested by t-test at the 0.05 significance level if it was significantly different from isometric growth.The total time spent on feeding either on mussel tissue or live mussels was recorded for each individual under controlled conditions in laboratory.Results:The length-weight relationships showed positive allometric growth and no inter-sex variability.Body size in the male population was significantly higher than in the individuals of the female.All characters in males and females showed a trend towards allometry rather than isometry.While the total time spent feeding increased with increasing prey size the total time that Rapana venosa spent feeding decreased with increasing Rapa whelk size.The total average feeding time needed by Rapa whelks was 160 min.But they took 310 min on live mussels in 27-28℃ in the laboratory conditions.Conclusions:Length and weight relationships,growth type,total time spent feeding of this species were explained in details for this region.It would be useful to sustainable management in the south-eastern Black Sea of Turkey.The results about the feeding behaviour of this species will contribute to the understanding of the role of this species within the ecosystem.展开更多
文摘在Pirkle型的 (S ,S) Whelk O 1与纤维素衍生物型的CDMPC两种手性柱上对六种外消旋萘普生酯进行了对映体分离 ,通过研究烷氧基结构上的差异以及流动相中不同的醇类添加剂对手性识别的影响 ,探讨和比较了外消旋萘普生酯在两种手性固定相上手性识别的机理 .对于 (S ,S) Whelk O 1,溶质与固定相之间的吸引作用是手性识别的主要因素 ,而对于CDMPC 。
基金supported by the National Natural Science Foundation of China(Grant Nos.32002409,31972814,32002374)the Natural Science Foundation of Shandong Province(Grant No.ZR2019BD003)+6 种基金the China Postdoctoral Science Foundation(Grant No.2021M703248)Young Elite Scientists Sponsorship Program by CAST,2021QNRC001earmarked fund for the Modern Agro-industry Technology Research System(CARS-49)the Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program(2019JZZY020708)the Industry Leading Talents Project of Taishan Scholars(Recipient:Tao Zhang)the“Double Hundred”Blue Industry Leader Team of Yantai(Recipient:Tao Zhang)the Creative Team Project of the Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology(no.LMEESCTSP-2018-1).
文摘Metamorphosis is the short developmental stage characterized by dramatic ontogenetic changes that occurs in most animals.However,this important process remains largely unclear in marine invertebrates.In this study,we performed the sequential RNA sequencing of a representative mollusk,the rapa whelk(Rapana venosa),that is undergoing metamorphosis and conducted differential gene expression analysis and weighted gene co-expression network analysis(WGCNA)to investigate the overall and dynamic transcriptome responses.The results revealed that the expression of cytochrome P4502A and 3A were upregulated during metamorphosis,while the expression of H/ACA ribonucleoproteins increased 4 h after metamorphosis induction(M4 stage),indicating that R.venosa mainly responded to the pelagobenthic changes.At the M24 stage,the enrichment of V-type proton ATPase and insulin indicated the complete development of secretory organs and initiation of hormone secretion.Furthermore,at the M48 stage,the enrichment of zinc metalloproteinase and conotoxin indicated a well-developed predation system that requires exogenous nutrition.Finally,during the PL stage,the genes associated with growth control were highly enriched,implying that R.venosa had completed metamorphosis and has entered the period of rapid growth.Therefore,our study provides useful transcriptomic resources for R.venosa and contributes new insights that may assist in elucidating the mechanisms underlying metamorphosis in marine invertebrates.
基金supported by the Karadeniz Technical University,Trabzon,Turkey.
文摘Objective:To determine length-weight relationships,growth type and feeding behavior of the benthic predator Rapa whelk at the coast of Camburnu,south-eastern Black Sea.Methods:Rapa whelk was monthly collected by dredge sampling on the south-eastern Black Sea at 20 m depth.The relationships between morphometric parameters of Rapa whelk were described by linear and exponential models.The allometric growth of each variable relative to shell length(SL)was calculated from the function Y=aSL^(b) or logY=loga_(+)blogSL.The functional regression b values were tested by t-test at the 0.05 significance level if it was significantly different from isometric growth.The total time spent on feeding either on mussel tissue or live mussels was recorded for each individual under controlled conditions in laboratory.Results:The length-weight relationships showed positive allometric growth and no inter-sex variability.Body size in the male population was significantly higher than in the individuals of the female.All characters in males and females showed a trend towards allometry rather than isometry.While the total time spent feeding increased with increasing prey size the total time that Rapana venosa spent feeding decreased with increasing Rapa whelk size.The total average feeding time needed by Rapa whelks was 160 min.But they took 310 min on live mussels in 27-28℃ in the laboratory conditions.Conclusions:Length and weight relationships,growth type,total time spent feeding of this species were explained in details for this region.It would be useful to sustainable management in the south-eastern Black Sea of Turkey.The results about the feeding behaviour of this species will contribute to the understanding of the role of this species within the ecosystem.
