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Genetic Analysis of Quantitative Signs of White Lupin (Lupinus albus L.) Genotypes
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作者 Valentin Kosev Viliana Vasileva 《Journal of Harbin Institute of Technology(New Series)》 CAS 2021年第4期84-96,共13页
Genetic variability in the base population plays an important role in crop⁃breeding program.A comprehensive assessment on the quantitative signs of white lupin genotypes was performed to be included in the selection p... Genetic variability in the base population plays an important role in crop⁃breeding program.A comprehensive assessment on the quantitative signs of white lupin genotypes was performed to be included in the selection process.Two limits of the environment were applied,i.e.,dense and sparse sowing.Positive true heterosis was established in PI533704×Zuter and PI533704×Lucky801 crosses by using the traits of the weight of nodules and fresh root mass weight at both limits of the environment.In more favorable growing conditions,the number and weight of nodules,as well as fresh aboveground mass weight had greater influence on the inheritance of epistatic gene interactions.In the case of dense sowing,the dominant gene actions for the weight of seeds were found more significant.With a high coefficient of inheritance in both environments,the hybrids of PI533704×Zuter and Lucky801×PI533704 were characterized by number and weight of nodules,Zuter×PI533704 by fresh root and aboveground mass weight,and almost all hybrids by seed weight per plant.The assessment of the initial material makes it highly likely to speed up the process of creating new varieties of white lupin. 展开更多
关键词 white lupin degree of dominance epistasis mass team mass selection INHERITANCE
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The Chemical Quality of Some Legumes, Peas, Fava Beans, Blue and White Lupins and Soybeans Cultivated in Finland
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作者 Marketta Saastamoinen Merja Eurola Veli Hietaniemi 《Journal of Agricultural Science and Technology(B)》 2013年第2期92-100,共9页
The cultivation and management of different legume species and their varieties were tested in South-Western Finland at farmers' fields in two years, 2010 and 2011. The studied legumes were peas and lava beans, and in... The cultivation and management of different legume species and their varieties were tested in South-Western Finland at farmers' fields in two years, 2010 and 2011. The studied legumes were peas and lava beans, and included also such species which are seldom or not normally cultivated in Finland, namely soybeans and blue and white lupins. Seed samples were collected from legume farms. The quality of different legume species was analysed and compared to each others. The chemical qualities were analysed for crude protein, fat, fibre, ash, nitrogen-free extract and phytic acid contents, and amino acid composition. The quality of the varieties of lupins and soybeans were compared to the quality of pea and fava bean varieties, which are generally cultivated nowadays in Finland. The highest protein contents were in soybean (369 and 379 g kg^-1) and white lupin (382 g kg^-1) and the lowest in pea varieties. Amino acid composition of legumes is good, but lupins have lower lysine content (g 100 g^-1 protein) than other legumes. High fat contents were in soybeans (91 and 100 g kg^-1) and white lupin (101 g kgl), and moderate in blue lupin (30-49 g kg^-1). Fuego fava bean variety had lower protein content than Kontu. The highest phytic acid contents were in soybeans (18.9 and 22.6 mg kg^-1) and the lowest in white lupin (6.2 mg kg^-1). Lupins are interesting protein crops, especially white lupin with high protein and fat and low phytic acid contents. Lupins are not day length sensitive, like soybean, and therefore valuable for long-day cultivation conditions The present research strengthens the good chemical quality of legumes for food and feed purposes. 展开更多
关键词 Protein fat amino acids phytic acid PEAS fava beans SOYBEANS blue and white lupins
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Root Cluster Formation and Citrate Exudation of White Lupin (Lupinus albus L.) as Related to Phosphorus Availability 被引量:4
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作者 Chun-JianLI Rui-XiaLIANG 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2005年第2期172-177,共6页
Abstract: A split-root system was used to investigate whether the external or internal P concentration controls root cluster formation and citrate exudation in white lupin (Lupinus albus L.) grown under controlled con... Abstract: A split-root system was used to investigate whether the external or internal P concentration controls root cluster formation and citrate exudation in white lupin (Lupinus albus L.) grown under controlled conditions. In spite of low P concentrations in the shoots and roots of the -P plant, its dry weight was not reduced compared with the +P plant. Supplying external P (0.25 mmol/L) to one root half resulted in an increase in P concentration not only in the shoot, but also in the P-deprived root half, indicating P cycling within the plants. Omitting P from both split-root pots stimulated root cluster formation in both root halves, whereas P supply to one root half stimulated root cluster formation at the beginning of the treatment. Neither P supply to just one root half continuously nor resupply of P to one root half after 19 d of P starvation inhibited root cluster formation on the P-deprived side, although the concentration of P in this root half and shoot increased markedly. The results indicate that root cluster formation in L. albus is controlled by both shoot and root P concentrations. The rates of citrate exudation by both root halves with P deficiency were higher than those of the one root half supplied with P only. In the treatment with one root half supplied with P, the rates of citrate exudation by either the P-supplied or -deprived root halves were almost the same, regardless of P concentration in the roots. The results suggest that internal P concentration controls root cluster formation and citrate exudation in white lupin, but these processes may be regulated by different mechanisms. 展开更多
关键词 citrate exudation P cycling root clusters split-root system white lupin (lupinus albus L.)
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Development and Characterization of Extruded Broken Rice and Lupine (<i>Lupinus albus</i>) 被引量:2
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作者 Cíntia Tarabal Oliveira érika Maria Roel Gutierrez +3 位作者 Márcio Caliari Márcia Regina Pereira Monteiro Renata Adriana Labanca Raquel Linhares Carreira 《American Journal of Plant Sciences》 2015年第12期1928-1936,共9页
Mixtures of cereals and legumes result in products with higher content of high biological value proteins. The thermoplastic extrusion promotes the transformation of chemical, physical and nutritional characteristics o... Mixtures of cereals and legumes result in products with higher content of high biological value proteins. The thermoplastic extrusion promotes the transformation of chemical, physical and nutritional characteristics of the food such as starch gelatinization and promotes the inactivation of anti-nutritional compounds and enzymes. This study aimed to develop products extruded using broken rice and lupine and to evaluate the physical and chemical characteristics of those products. The expansion index (EI), water solubility (WSI), water absorption (WAI), texture and color were determined for the extruded rice, and Scanning Electron Microscopy (SEM) of the extruded rice was also performed. As the rice concentration in the mixture increased, the EI, WSI and WAI values as well as the a* color parameter were increased too, and the brightness was reduced. An increase in the levels of essential amino acids with the addition of lupine grain to the extrudates was observed. SEM analysis identified intact loose starch granules or pellets in the raw materials, and the extruded samples showed a compact and amorphous shape without apparent starch granules. Due to the structural changes and reduced expansion rate of the extruded mixture prepared with lupine and rice grits, use of the extruded mixtures as snacks would not be suitable. However, these mixtures could be used in pre-gelatinized flour to formulate various products to increase the protein level. 展开更多
关键词 Technological white Rice white lupinE Thermoplastic Extrusion STARCH
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缺磷条件下白羽扇豆排根发育与生长素及miR164的关系 被引量:6
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作者 朱毅勇 曾后清 +2 位作者 董彩霞 尹晓明 沈其荣 《西北植物学报》 CAS CSCD 北大核心 2010年第2期317-322,共6页
以缺磷条件下白羽扇豆为材料,观察了外源生长素NAA和生长素运输的抑制剂NPA对白羽扇豆排根形成及其活性的影响,同时运用基因芯片与RT-PCR的方法分析了生长素信号转导途径中转录因子NAC1以及调控NAC1表达的上游microRNA164(miR164)在不... 以缺磷条件下白羽扇豆为材料,观察了外源生长素NAA和生长素运输的抑制剂NPA对白羽扇豆排根形成及其活性的影响,同时运用基因芯片与RT-PCR的方法分析了生长素信号转导途径中转录因子NAC1以及调控NAC1表达的上游microRNA164(miR164)在不同发育阶段排根中的表达变化,以探讨白羽扇豆在缺磷时排根形成与发育的调控机制。结果表明,缺磷胁迫下排根大量形成与生长素及其运输有关,排根NAC1的表达在初生阶段上调,成熟后下调,并受其上游的miR164的负调控,而排根衰老后则上述基因的表达都减弱。研究发现,在缺磷诱导的排根发生至发育成熟过程中,miR164、NAC1、生长素与排根发育之间很可能组成了一个级联系统,从而控制排根的发生与发育。 展开更多
关键词 白羽扇豆 排根 生长素 miR164 NAC1
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白羽扇豆缺磷胁迫下miR399与磷响应基因的表达及关系 被引量:3
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作者 曾后清 朱毅勇 +2 位作者 尹晓明 董彩霞 沈其荣 《土壤学报》 CAS CSCD 北大核心 2010年第5期971-978,共8页
通过microRNA(miRNA)基因芯片及RT-PCR研究了白羽扇豆在缺磷胁迫下miR399与磷响应基因的表达变化。结果表明:缺磷处理后根系生物量显著高于供磷处理,但地上部生物量降低,并且植株体内磷含量明显减少。基因芯片结果表明,缺磷白羽扇豆根... 通过microRNA(miRNA)基因芯片及RT-PCR研究了白羽扇豆在缺磷胁迫下miR399与磷响应基因的表达变化。结果表明:缺磷处理后根系生物量显著高于供磷处理,但地上部生物量降低,并且植株体内磷含量明显减少。基因芯片结果表明,缺磷白羽扇豆根、茎和叶中分别有10、7和3个不同成员的miR399s表达上调,平均上调倍数分别为4.4,3.8和2.5。6个磷响应基因LaATPase、LaPT1、LaMATE、La-PEPC3、LaSAP和LaMDH1在缺磷排根中的表达均高于供磷侧根,启动子序列分析表明LaPT1和LaMATE启动子区域有与PHR1或WRKY转录因子结合的磷响应元件。在此基础上得出有关miR399,PHR1与这些受缺磷诱导的基因之间的调控关系。研究表明,miR399和磷响应基因对白羽扇豆适应缺磷环境起着重要作用。 展开更多
关键词 白羽扇豆 缺磷 miR399 基因表达
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6-BA对缺磷白羽扇豆排根形成和有机酸分泌的影响 被引量:16
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作者 梁瑞霞 李春俭 宋建兰 《植物生理与分子生物学学报》 CAS CSCD 2004年第6期619-624,共6页
缺磷条件下白羽扇豆能够形成排根,并增加有机酸分泌。但上述过程的调节机制尚不清楚。该文的结果表明,使用外源6-BA不影响缺磷白羽扇豆的生长和磷在体内的分配,但明显抑制了根簇的形成和有机酸分泌。经低浓度6-BA(10-8mol/L)处理后转... 缺磷条件下白羽扇豆能够形成排根,并增加有机酸分泌。但上述过程的调节机制尚不清楚。该文的结果表明,使用外源6-BA不影响缺磷白羽扇豆的生长和磷在体内的分配,但明显抑制了根簇的形成和有机酸分泌。经低浓度6-BA(10-8mol/L)处理后转移至不含6-BA的缺磷营养液中继续培养的植株,其根簇形成和有机酸分泌得到恢复,甚至超过未经6-BA处理的缺磷植株;但高浓度6-BA(10-7mol/L)对根簇形成和有机酸分泌的抑制作用不可恢复。对6-BA影响缺磷的白羽扇豆排根形成和有机酸分泌的可能机制进行了讨论。 展开更多
关键词 缺磷 白羽扇豆 排根 有机酸分泌 6-BA(6-苄基腺嘌呤)
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不同羽扇豆锰吸收差异及其机制探讨
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作者 丁玉川 聂督 《西北植物学报》 CAS CSCD 北大核心 2009年第2期302-307,共6页
以白羽扇豆(Lupinus albus L.cv. Kiev)和窄叶羽扇豆(Lupinus angustifolius L.cv. Gungurru)为材料,采用溶液培养方法,在玻璃温室条件下对2种羽扇豆在高锰(10μmol/L)和低锰(0.5μmol/L)环境中的锰吸收差异及其可能机制进行分析.结果表... 以白羽扇豆(Lupinus albus L.cv. Kiev)和窄叶羽扇豆(Lupinus angustifolius L.cv. Gungurru)为材料,采用溶液培养方法,在玻璃温室条件下对2种羽扇豆在高锰(10μmol/L)和低锰(0.5μmol/L)环境中的锰吸收差异及其可能机制进行分析.结果表明,无论在高锰还是低锰条件下,白羽扇豆植株地上部和根系的锰吸收和转运能力、木质部和韧皮部汁液中的锰含量及低分子量有机酸含量在不同生长阶段都显著高于窄叶羽扇豆,且2种羽扇豆植株韧皮部汁液中锰含量与其低分子量有机酸含量呈显著正相关.研究发现,2种羽扇豆的吸锰能力差异与其种子大小、种子中锰含量、锰的吸收和转运能力以及木质部和韧皮部低分子量有机酸含量有密切关系;锰离子与低分子有机酸形成的可溶性有机复合体可能是促进锰在植物韧皮部转移的一个重要机制. 展开更多
关键词 白羽扇豆 窄叶羽扇豆 锰吸收 木质部和韧皮部汁液 低分子量有机酸
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缺磷或缺铁引起白羽扇豆锰中毒的机理 被引量:2
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作者 陆萍 张福锁 《植物生理学报(0257-4829)》 CSCD 1995年第3期289-294,共6页
白羽扇豆在缺磷或缺铁条件下均有排根形成,并且根系还原力显著增加。缺磷、缺铁根系还原力在高峰期分别高于对照。缺磷与缺铁根系还原力高峰不仅出现的时期不同,而且还原力增加部位也不一样。缺磷处理的排根区具有很高的还原力,缺铁... 白羽扇豆在缺磷或缺铁条件下均有排根形成,并且根系还原力显著增加。缺磷、缺铁根系还原力在高峰期分别高于对照。缺磷与缺铁根系还原力高峰不仅出现的时期不同,而且还原力增加部位也不一样。缺磷处理的排根区具有很高的还原力,缺铁处理还原力较高的部位是在主根和侧根的根尖以及排根区。由于Mn4+比Fe3+更易被还原,致使根系还原力提高促使根际大量锰被还原,这是缺磷和缺铁造成白羽扇豆锰中毒的主要原因之一。 展开更多
关键词 白羽扇豆 缺磷 缺铁 锰中毒
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白羽扇豆WRKY转录因子家族鉴定及生物信息学分析
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作者 亚华金 沈莲文 +3 位作者 周涛 李明谦 余文才 和丽岗 《热带农业科学》 2022年第12期42-53,共12页
WRKY转录因子家族在植物生命活动中具有重要作用。利用生物信息学方法分析白羽扇豆基因组数据,对LaWRKY转录因子家族成员进行鉴定及染色体定位,同时分析其蛋白理化性质、系统发育进化关系、保守基序分布、保守结构域位置、基因结构及启... WRKY转录因子家族在植物生命活动中具有重要作用。利用生物信息学方法分析白羽扇豆基因组数据,对LaWRKY转录因子家族成员进行鉴定及染色体定位,同时分析其蛋白理化性质、系统发育进化关系、保守基序分布、保守结构域位置、基因结构及启动子-顺式作用元件等,并从系统发育进化的同源性、启动子-顺式作用元件等多角度对LaWRKY转录因子功能进行预测。结果表明,在白羽扇豆中共鉴定到106个LaWRKY转录因子家族成员,各成员不均匀地分布在24条染色体上,但18号染色体上无分布;106个成员聚类为3个亚族(Ⅰ、Ⅱ、Ⅲ),各亚族成员的保守基序分布相似,对应的保守结构域、基因结构特征也表现一定的规律性,多角度预测到LaWRKY转录因子与植物激素、环境变化等方面有关联。本研究为深入解析LaWRKY转录因子对白羽扇豆生命活动的调控作用提供理论参考。 展开更多
关键词 白羽扇豆 基因组 WRKY转录因子 生物信息学
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发根农杆菌介导白羽扇豆转基因过表达体系的建立 被引量:5
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作者 詹玉洁 刘博文 +2 位作者 张仟 许卫锋 夏天雨 《植物生理学报》 CAS CSCD 北大核心 2021年第3期655-660,共6页
白羽扇豆(Lupinus albus)是研究耐低磷机制的模式作物,然而遗传转化体系的缺乏极大限制了其功能基因组学研究。本试验以白羽扇豆品种‘Amiga’为材料,研究不同发根农杆菌菌株对白羽扇豆毛状根诱导率和阳性转化率的影响。结果发现K599为... 白羽扇豆(Lupinus albus)是研究耐低磷机制的模式作物,然而遗传转化体系的缺乏极大限制了其功能基因组学研究。本试验以白羽扇豆品种‘Amiga’为材料,研究不同发根农杆菌菌株对白羽扇豆毛状根诱导率和阳性转化率的影响。结果发现K599为白羽扇豆发根遗传转化的最适菌种,进而确立了一套高效的白羽扇豆毛状根转化体系,并成功获得增强型绿色荧光蛋白基因EGFP (ENHANCED GREEN FLUORESCENT PROTEIN)在白羽扇豆毛状根中过表达的嵌合植株。该遗传转化体系的建立为白羽扇豆功能基因组学研究提供了强有力的方法支撑。 展开更多
关键词 白羽扇豆 发根农杆菌 毛状根 遗传转化 嵌合植株
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