Network pharmacology and subsequent experimental validation reveal the synergistic myocardial protection mechanism of Salvia miltiorrhiza Bge.and Carthamus tinctorius L.

Objective:To reveal the molecular mechanism underlying the compatibility of Salvia miltiorrhiza Bge(S.miltiorrhiza,Dan Shen)and C.tinctorius L.(C.tinctorius,Hong Hua)as an herb pair through network pharmacology and subsequent experimental validation.Methods:Network pharmacology was applied to construct an active ingredient-efficacy target-disease protein network to reveal the unique regulation pattern of s.miltiorrhiza and C.tinctorius as herb pair.Molecular docking was used to verify the binding of the components of these herbs and their potential targets.An H9c2 glucose hypoxia model was used to evaluate the efficacy of the components and their synergistic effects,which were evaluated using the combination index.Western blot was performed to detect the protein expression of these targets.Results:Network pharmacology analysis revealed 5 pathways and 8 core targets of s.miltiorrhiza and C.tinctorius in myocardial protection.Five of the core targets were enriched in the hypoxia-inducible factor-1(HIF-1)signaling pathway.S.miltiorrhiza-C.tinctorius achieved vascular tone mainly by regulating the target genes of the HIF-1 pathway.As an upstream gene of the HIF-1 pathway,STAT3 can be activated by the active ingredients cryptotanshinone(Ctan),salvianolic acid B(Sal.B),and myricetin(Myric).Cell experiments revealed that Myric,Sal.B,and Ctan also exhibited synergistic myocardial protective activity.Molecular docking verified the strong binding of Myric,Sal.B,and Ctan to STAT3.Western blot further showed that the active ingredients synergistically upregulated the protein expressionof STAT3.Conclusion:The pharmacodynamic transmission analysis revealed that the active ingredients of S.miltiorrhiza and C.tinctorius can synergistically resist ischemia through various targets and pathways.This study provides a methodological reference for interpreting traditional Chinese medicine compatibility.

Detection of Prorocentrum donghaiense using sandwich hybridization integrated with nuclease protection assay

Prorocentrum donghaiense is an important harmful algae bloom （HAB） causing creature in China＇s seas, and the conventional visual detection can not cope with long-term monitoring and highthroughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay （NPA-SH） was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, and the regression equation is y=4× 10^- 6x＋ 0.694 9, in which x is the cell number, and y is the optical density, with r2=0.953 5. These results show that the NPA-SH method has good feasibility in the detection of P. donghaiense. Results of NPA-SH and microscopy are excellent for each sample. The NPA-SH method was a simple way in quantitative detection of P. donghaiense, and the whole process could be finished in about six hours, which provided a new approach in high-throughput sampling and long-term monitoring of P. donghaiense.

Designing a Need Based Social Protection Intervention Package for Children and Adolescents Living with HIV and AIDS in Ghana—An Eclectic Perspective on Desired Social Protection Intervention Package/Framework

Background: Designing a need-based social protection intervention for children comes as comprehensive tool for addressing the overall quality of life of HIV positive children. Not much has been examined on what constitute the desired social protection framework for HIV positive children. Methods: This study was informed by a qualitative descriptive exploratory design approach. The study drew insights from the shared perspectives of 27 participants sampled from positive HIV status disclosed children—10 - 17 years, healthcare workers, Social and Development worker, HIV positive caregivers and parents from 7 ART clinics in rural and urban Brong Ahafo and Ashanti regions of Ghana. Data was collected through interviews and two focus group discussions with study participants. Drawing on the emerged themes from the transcripts, thematic content analysis was used to analyze the data using comparative thematic framework approach. Findings: The eclectic perspectives on the type of social protection policy/intervention preferred demonstrated the need for an interrelated and integrated social protection policy. This should not be designed in isolation towards addressing the child vulnerability. The study found that social protection policies aimed at addressing vulnerability levels of children should consider the dual vulnerability situation within which HIV positive children and adolescents are located. A set of unique specific tailored approach and package reflecting minimum combination social protection package to HIV positive children was considered essential for inclusion. The themes that emerged covered social policy/intervention package inclusive of a disease ending/eradication intervention package, cash transfer for children and their caregivers, free comprehensive health insurance integrating all consultancy and laboratory services charges, direct food supplements supply and nutritional support, research, target specified with evidenced-based monitoring. Counseling support and preferential treatment for children visiting ART for treatment were to be included. Conclusion: Combination social protection intervention packages remain the pathway to yielding maximum dividends on any social intervention that seeks to address the vulnerability levels of HIV positive children in Ghana. This should be designed taking into consideration the preference of the main target beneficiaries (HIV positive children) while accounting for the inclusion of the technical expertise of implementing stakeholders in the policy design from conceptualization to evaluation.

A Double-Ended Protection Principle for an LCC-VSC-MTDC System with Strong Anti-Interference Ability

The DC grid technology of multi-power supply and multi-drop-point power reception is an effective solution for large-scale renewable energy integration into the power grid.Line-commutated converter-Voltage source converter(LCC-VSC)power grids are one of the more important developmental directions of the future power grid that have occured in recent years.But the multi-terminal high voltage direct current system has the problems of inconsistent boundary characteristics,inconsistent control,and fault response characteristics,which puts higher requirements on the protection scheme.Thus,a completely new protection principle is proposed in this paper.Firstly,the fault characteristics of distributed capacitance current are analyzed.The reactive power calculated by the distribution parameters of different frequencies is different.Subsequently,the fault characteristics of DC reactive power are analyzed,and a DC reactive power extraction algorithm is proposed.The polarity of the multi-band DC reactive power is used to construct the protection scheme.Finally,the LCC-VSC power grid model verifies the correctness and superiority of the proposed protection scheme.The proposed scheme uses DC reactive power instead of fault current to solve the long delay problem caused by distributed capacitance.Compared with the prior art,the proposed solution is not affected by distributed capacitance and has a stronger anti-interference ability(600Ω+10 dB+1 ms).

The International Protection of Women's Human Rights:with Emphasis on UN Convention on the Elimination of All Forms of Discrimination against Women

1. The International Protection of Women＇s Human Rights： Emergence of CEDAW For a long time, women＇s rights have been excluded from traditional discourse on human right. The traditional discourse on human rights has developed without considering its impact upon women. Transforming this discourse to a perspective that will consider the needs and vindications of women is absolutely essential.1 Although international efforts addressing women＇s issues presumed to be associated with women began in the nineteenth century, it was in the postwar period that such activity gained a coherent focus under the auspices of United Nations.

Protection of Human Rights Under China's Legal System——An interview with Prof. Wan Exiang

Wan Exiang, professor of the Law School of Wuhan University, doubles as justice and vice-president of China's Supreme People's Court. He is also vice-chairman of the Revolutionary Committee of the Chinese Kuomintang, one of the democratic parties in China, and member of the Tenth National Committee of the Chinese People's Political Consultative Conference, China's highest consulting body. Prof. Wan is reputed in China and abroad for his achievements in study of international human rights laws and also as the founder of the Wuhan University Center for Protection of the Rights of the Underprivileged in Society, the first of its kind ever set up in the country. Since its founding a decade ago, the center has won a fame for the quality legal services it renders to needy people in their tens of thousands, including disabled people, juveniles, women and workers laid off from state-owned enterprises in the course of China's economic restructuring. While a press favorite at home, the center has received coverage by the NHK TV of Japan, Washington Post of the United States, Phoenix TV of Hong Kong and numerous other media outside the Chinese mainland. Following is an interview with Prof. Wan Exiang, in which he discussed how human rights are protected under China's legal system and in what ways the system should be improved.

Human Rights Protection Under China's Judicial System——An Interview with Xiao Yang, President of the Supreme People's Court of the People's Republic of China

Question: As you know. attention is now growing in China and outside to judicial protection of human rights. As President of the Supreme People’s Court and China’s top judge, would you make some comments on protection of human rights under China’s juridical system?

Protection of Rights Ensures Women's Development--An interview with Liu Qunying,chair of Fujian Women's Federation

Editor's note: Following is the interview with Liu Qunying, chair of the Women's Federation of Fuijian Province, about how women's rights and interests are protected in this southeastern province.

Research on Monitoring and Early-warning Standards of Ancient and Famous Trees Protection in the Humble Administrator's Garden

Based on the current situation and symptoms of the trees' growth in the Humble Administrator's Garden,this paper put forward corresponding monitoring and early-warning standards and technical measures of the ancient and famous trees protection in the Humble Administrator's Garden specifically.The aim of doing this is to establish a scientific basis for the protection of the ancient and famous trees in the Humble Administrator's Garden by setting up systematic fundamental data,dynamic protection standard grades and technique measures of protecting the trees.The main symptom of trees in the Humble Administrator's Garden is the erosion and decay of the tree trunks.Fifteen tree trunks need technical protection,which holds 65.22% of the total sum of trees in the Humble Administrator's Garden.Therefore,much more emphasis should be paid in strengthening technical protection procedures of monitoring and early warning of the tree trunks in the future protection of the ancient and famous trees in the garden.Besides,the rejuvenation technique of rooting zone and rooting system,tree pruning technique as well as tree supporting measures according to the specific condition and symptom of the trees should be concerned with in order to protect the ancient and famous trees in the Humble Administrator's Garden in a more scientific and effective way.

Reform of China's Judicial System Promotes Protection of Human Rights——An interview with Wang Shengjun, secretary-general of the Politico-Legal Committee of the CPC Central Committee

The 16th National Congress of the Communist Party of China （CPC） held in 2003 set thetask of promoting the reform of China＇s judicial system, a task seen as of strategic importance to the country＇s development. Shortly afterwards, the CPC Central Committee set up the Leading Group for the Judicial System Reform （LGJSR）.

Regulation on the Protection of Fossils Issued by the State Council of the People's Republic of China:Scientists' Researches Protected Legally—Further Explanation

With large-scale engineering projects being carried out in China, a large number of fossil localities have been discovered and excavated by responsible agencies, but still some important fossils of great value have been removed and smuggled into foreign countries. In the last three years, more than 1345 fossil specimens have been intercepted by Customs in Shenzhen, Shanghai, Tianjin, Beijing and elsewhere, and more than 5000 fossils, most of which are listed as key fossils,

A Protection Method of VSC-HVDC Cables Based on Generalized S-Transform

Generalized S-transform is a time-frequency analysis method which has higher resolution than S-transform. It can precisely extract the time-amplitude characteristics of different frequency components in the signal. In this paper, a novel protection method for VSC-HVDC (Voltage source converter based high voltage DC) based on Generalized S-transform is proposed. Firstly, extracting frequency component of fault current by Generalized S-transform and using mutation point of high frequency to determine the fault time. Secondly, using the zero-frequency component of fault current to eliminate disturbances. Finally, the polarity of sudden change currents in the two terminals is employed to discriminate the internal and external faults. Simulations in PSCAD/EMTDC and MATLAB show that the proposed method can distinguish faults accurately and effectively.

THE DEVELOPMENT AND PROTECTION OF WETLANDS IN THE FOUR LAKE AREA OF JIANGHAN PLAIN

This paper summaries the ecological and environmental research works in the Four Lake Area. A great deal of research and developing works have been done, which may be concluded into three kinds of models for a agricultural development. They are Model Ⅰ-d

Children witness a systematic research

in China, children witness has not been articled in three procedural laws, some scholar do not think that either children nor other person with no legal capacity should have witness qualification.in fact, although some other countries do not admit the qualification of children witnesses under certain age,they do not take it as the factor to measure whether a witness has qualification or not.It is the judge who can decide whether a witness has qualification or not.Based on the these foreign counlries＇ s experience,we should not limit the qualification of children witnesses in the legislation. However, for the particularity of children＇s cognitive ability,we should embody this particularity when we judge children＇s testimony and protect children witnesses.

Children witness --a systematic research

In China, children witness has not been articled in three procedural laws, some scholar do not think that either children nor other person with no legal capacity should have witness qualification.In fact, although some other countries do not admit the qualification of children witnesses under certain age, they do not take it as the factor to measure whether a witness has qualification or not. It is the judge who can decide whether a witness has qualification or not. Based on the these foreign countries＇s experience, we should not limit the qualification of children witnesses in the legislation. However, for the particularity of children＇s cognitive ability, we should embody this particularity when we judge children＇s testimony and protect children witnesses.

China's Labor and Social Security Protection under the Globle Financial Crisis

Against the background of economic globalization, the economy of many countries has been inevitably affected by the globle financial crisis. But the impact varies. China, as one of the leading countries in globalization, the direct or indirect impact it suffered can be imagined. The Chinese government adopted a series of measures to deal with the financial crisis.

Protection of Women's Rights Means Human Rights Protection——Marking the 30th anniversary of the Convention on the Elimination of All Forms of Discrimination against Women

On December 18, 1979, the 34th General Assembly of the United Nations adopted the Convention on the Elimination of All Forms of Discrimination against Women with an overwhelming majority of the votes in its favor. Over the past 30 years, the Convention has come to be known by increasing numbers of governments and people, particularly women＇s organizations. It has played an increasingly great role in protecting women＇s rights and enhancing women＇s status in society.

Legislative Protection for Women's Rights and Interests after the Founding of the People's Republic of China

The process of legislative protection for women’s rights and interests of the new China can be divided into two phases: The first phase is from the establishment of the People’s Republic of China to the adoption of the policy of reform and opening up to the outside

PROGRESS OF CHINA'S ENVIRONMENT PROTECTION

SincethelastAsia-PacificParliamentarian'sConferenceonEnvironmentandDevelopment,theChinesegovernmenthaskeptontheimplementingthesustainabledevelopmentstrategy.Thenationaleconomyhasenjoyedanincreasinggrowthmomentumwiththeadoptionofpositivefiscalpolicies...