A fluorescencein situ hybridization (FISH) procedure was adopted to physically map a RFLP marker, umc119 near the centromere of the long arm of linkage group1 in maize. The hm1 gene (Helminthosporium carbonum suscepti...A fluorescencein situ hybridization (FISH) procedure was adopted to physically map a RFLP marker, umc119 near the centromere of the long arm of linkage group1 in maize. The hm1 gene (Helminthosporium carbonum susceptibility gene) was linked closely with the marker umc119. RFLP markers are very good landmarks for mapping genes. Therefore, we also determined the position of the gene hm1 on the chromosome based on the physical location of umc119. The disease induced by infection ofHelminthosporium carbonum is one of the serious maize diseases and it distributes in many countries including China. Hybridization sites were showed on 1 L (long arm of chromosome1) and 5 L. The percentage distance from centromere to the hybridization site was 22.86 on 1 L and 58.23 on 5 L the detection rate was about 12% for mitotic cells. In interphase nuclei five hybridized sites were detected. It demonstrated that umc119 was multiplicated sequences. FISH has more advantages overin situ hybridization (ISH) detected by DAB for increasing the detection ratio and contrast between chromosomes and hybridization signals. The ability to detect the hybridization signal of a small low copy DNA sequence is a very important key towards wide application of FISH for plant genome mapping.展开更多
Based on a literature review from English language journals related to the field of human resource development(HRD),the conceptual framework for this study was derived from the models developed by American Society for...Based on a literature review from English language journals related to the field of human resource development(HRD),the conceptual framework for this study was derived from the models developed by American Society for Training and Development(ASTD)for HRD practice.This study compared and analyzed the similarities and differences in HRD roles,key work dimensions,and practitioner competencies through the competitive vision of different countries,including China,US,UK,Germany,Japan,India,Singapore,and Korea.This comparative study not only described the HRD position of Chinese enterprises under the international HRD coordinates but also indicated how these differences inspire HRD in China.展开更多
文摘A fluorescencein situ hybridization (FISH) procedure was adopted to physically map a RFLP marker, umc119 near the centromere of the long arm of linkage group1 in maize. The hm1 gene (Helminthosporium carbonum susceptibility gene) was linked closely with the marker umc119. RFLP markers are very good landmarks for mapping genes. Therefore, we also determined the position of the gene hm1 on the chromosome based on the physical location of umc119. The disease induced by infection ofHelminthosporium carbonum is one of the serious maize diseases and it distributes in many countries including China. Hybridization sites were showed on 1 L (long arm of chromosome1) and 5 L. The percentage distance from centromere to the hybridization site was 22.86 on 1 L and 58.23 on 5 L the detection rate was about 12% for mitotic cells. In interphase nuclei five hybridized sites were detected. It demonstrated that umc119 was multiplicated sequences. FISH has more advantages overin situ hybridization (ISH) detected by DAB for increasing the detection ratio and contrast between chromosomes and hybridization signals. The ability to detect the hybridization signal of a small low copy DNA sequence is a very important key towards wide application of FISH for plant genome mapping.
文摘Based on a literature review from English language journals related to the field of human resource development(HRD),the conceptual framework for this study was derived from the models developed by American Society for Training and Development(ASTD)for HRD practice.This study compared and analyzed the similarities and differences in HRD roles,key work dimensions,and practitioner competencies through the competitive vision of different countries,including China,US,UK,Germany,Japan,India,Singapore,and Korea.This comparative study not only described the HRD position of Chinese enterprises under the international HRD coordinates but also indicated how these differences inspire HRD in China.
