[Objectives]The paper was to analyze and predict the structure and characteristics of xylanase xynMF13-GH10 gene and its encoded protein.[Methods]xynMF13-GH10 gene was predicted by NCBI and various information analysi...[Objectives]The paper was to analyze and predict the structure and characteristics of xylanase xynMF13-GH10 gene and its encoded protein.[Methods]xynMF13-GH10 gene was predicted by NCBI and various information analysis tools in ExPASy website,as well as SignaIP 5.0,DNAman,TMHMM,SOPMA and SWISS-Model,and the characteristics and functions of protein structure encoded by the gene were predicted.[Results]The gene is 1332 bp in length,the coding region is 1-1332 bp,and the gene encodes 443 amino acids.The xynMF13-GH10 gene has high homology with xylanase in many species,and it has the highest homology with Paraphaeosphaeria sporulosa endoxylanase-like protein,with the consistency reaching 78.91%and e-value reaching 2e-159.The secondary structure consists of 48.31%random curl,30.25%α-helix structure,16.70%extended chain and 4.74%β-corner.[Conclusions]The results provide a reference for revealing the physiological function and expression regulation mechanism of xynMF13-GH10 gene in the future.展开更多
Through water cultivating method, the dynamic changes of xylanase activity in seed, root and plumule of wheat with different As （III） concentration treatment were studied. The results indicated that the order of ave...Through water cultivating method, the dynamic changes of xylanase activity in seed, root and plumule of wheat with different As （III） concentration treatment were studied. The results indicated that the order of average xylanase activity was seed〉plumule〉root. With the increasing concentration of As （III）, the xylanase activity elevated first then dropped in seed, but it descended first then ascended in root and plumule. As the sampling time prolonged, the xylanase activity of seeds climbed first then dropped on the four as （III） concentration, the same trend also appeared in pulume, as the as （Ill） concentration went up, the xylanase activity moved up simultaneity. Semi-quantity Reverse Transcription Polymerase Chain Reaction was used in the study, the results indicated that, the xylanase gene began to express at 132 h on 0 mg/L As （III） concentration and at 120h on other concentration in the leaves of wheat.展开更多
基金Public Service Platform Project for Industrial Chain Collaborative Innovation(ZHC 2017CB82)Public Service Platform Subproject for Research and Development of Marine Biomedical Resources in the South China Sea(XM-202008-01B2).
文摘[Objectives]The paper was to analyze and predict the structure and characteristics of xylanase xynMF13-GH10 gene and its encoded protein.[Methods]xynMF13-GH10 gene was predicted by NCBI and various information analysis tools in ExPASy website,as well as SignaIP 5.0,DNAman,TMHMM,SOPMA and SWISS-Model,and the characteristics and functions of protein structure encoded by the gene were predicted.[Results]The gene is 1332 bp in length,the coding region is 1-1332 bp,and the gene encodes 443 amino acids.The xynMF13-GH10 gene has high homology with xylanase in many species,and it has the highest homology with Paraphaeosphaeria sporulosa endoxylanase-like protein,with the consistency reaching 78.91%and e-value reaching 2e-159.The secondary structure consists of 48.31%random curl,30.25%α-helix structure,16.70%extended chain and 4.74%β-corner.[Conclusions]The results provide a reference for revealing the physiological function and expression regulation mechanism of xynMF13-GH10 gene in the future.
文摘Through water cultivating method, the dynamic changes of xylanase activity in seed, root and plumule of wheat with different As （III） concentration treatment were studied. The results indicated that the order of average xylanase activity was seed〉plumule〉root. With the increasing concentration of As （III）, the xylanase activity elevated first then dropped in seed, but it descended first then ascended in root and plumule. As the sampling time prolonged, the xylanase activity of seeds climbed first then dropped on the four as （III） concentration, the same trend also appeared in pulume, as the as （Ill） concentration went up, the xylanase activity moved up simultaneity. Semi-quantity Reverse Transcription Polymerase Chain Reaction was used in the study, the results indicated that, the xylanase gene began to express at 132 h on 0 mg/L As （III） concentration and at 120h on other concentration in the leaves of wheat.
