Objective:To evaluate the effect of ligust razine on bone marrow hematopoietic microenvironment and signal transduction in irradiation injured mice.Methods:After being irradiated by 6.0 Gy 60 Co γ -ray, the mi...Objective:To evaluate the effect of ligust razine on bone marrow hematopoietic microenvironment and signal transduction in irradiation injured mice.Methods:After being irradiated by 6.0 Gy 60 Co γ -ray, the mice in the ligustrazine group were orally given ligustrazine 4 mg/mouse twice a day to the end of the experiment. For the control group, normal saline was given instead of ligustrazine and the mice in the normal group was untreated. The mice were sacrificed separately on the 3rd, 7th and 14th day after radiation, bone marrow of them was taken and m anaged as follows:(1) Make stromal cell culture to observe the growth state and the expression of focal adhesion kinase (FAK) of the cells; (2) Make the bo ne marrow section to examine the pathological and immunohistochemistry changes, including the expression of fetal liver kinase-1 (Flk-1) and F VIII :RAg. The data were recorded and compared among groups.Results:After radiation, the hematopoietic cells in bo ne marrow decreased and the micro vessels dilated and congested with bleeding; bone mar row became thinner, red colored with cells of irregular shape and few cells adhere to the bottom of culture flask. These changes began to recover at the 7th day and approached to normal within 2 weeks. The recovery was better, earlier and quick er in the ligustrazine group than that in the control group. The expression lev els of Flk-1 decreased significantly.Conclusion: Ligustrazine could promote the recovery of hematopoietic function of radiation damaged bone marrow, the mechanisms might be through imp roving the microenvironment and signal transduction path for recovery.展开更多
文摘Objective:To evaluate the effect of ligust razine on bone marrow hematopoietic microenvironment and signal transduction in irradiation injured mice.Methods:After being irradiated by 6.0 Gy 60 Co γ -ray, the mice in the ligustrazine group were orally given ligustrazine 4 mg/mouse twice a day to the end of the experiment. For the control group, normal saline was given instead of ligustrazine and the mice in the normal group was untreated. The mice were sacrificed separately on the 3rd, 7th and 14th day after radiation, bone marrow of them was taken and m anaged as follows:(1) Make stromal cell culture to observe the growth state and the expression of focal adhesion kinase (FAK) of the cells; (2) Make the bo ne marrow section to examine the pathological and immunohistochemistry changes, including the expression of fetal liver kinase-1 (Flk-1) and F VIII :RAg. The data were recorded and compared among groups.Results:After radiation, the hematopoietic cells in bo ne marrow decreased and the micro vessels dilated and congested with bleeding; bone mar row became thinner, red colored with cells of irregular shape and few cells adhere to the bottom of culture flask. These changes began to recover at the 7th day and approached to normal within 2 weeks. The recovery was better, earlier and quick er in the ligustrazine group than that in the control group. The expression lev els of Flk-1 decreased significantly.Conclusion: Ligustrazine could promote the recovery of hematopoietic function of radiation damaged bone marrow, the mechanisms might be through imp roving the microenvironment and signal transduction path for recovery.
