Due to limited resources and experience, rapid diagnostic techniques are advocated in nations with a resource shortage when diagnosing schistosomiasis. We used rapid diagnostic tests to access the prevalence and inten...Due to limited resources and experience, rapid diagnostic techniques are advocated in nations with a resource shortage when diagnosing schistosomiasis. We used rapid diagnostic tests to access the prevalence and intensity of schistosome infection in North Central, Nigeria. A total of 1951 participants were recruited for this study. The participants were screened for S. haematobium infection;haematuria and proteinuria were monitored in the recruited patients with a commercial reagent strip. Of the 1951 participants recruited for the study, 587 were found to be infected. Children aged 0 to 10 years showed the highest levels of haematuria with (100%) specificity. Meanwhile, other age groups (11 - 20, 21 - 30, 31 - 40 and above 40 years) had rates higher than 90%. The degree of haematuria increased with egg intensity. The same was seen in proteinuria, with a percentage of 41.9%. A significant difference (p S. haematobium in rural endemic areas.展开更多
Objective To study the anticlastogenic effect of redistilled cow＇s urine distillate （RCUD） in human peripheral lymphocytes （HLC） challenged with manganese dioxide and hexavalent chromium. Methods The anticlastoge...Objective To study the anticlastogenic effect of redistilled cow＇s urine distillate （RCUD） in human peripheral lymphocytes （HLC） challenged with manganese dioxide and hexavalent chromium. Methods The anticlastogenic activity of redistilled cow＇s urine distillate was studied in human polymorphonuclear leukocytes （HPNLs） and human peripheral lymphocytes in vitro challenged with manganese dioxide and hexavalent chromium as established genotoxicants and clastogens which could cause induction of DNA strand break, chromosomal aberration and micronucleus. Three different levels of RCUD： 1 μL/mL, 50 μL/mL and 100 μL/mL, were used in the study. Results Manganese dioxide and hexavalent chromium caused statistically significant DNA strand break, chromosomal aberration and micronucleus formation, which could be protected by redisfilled cow＇s urine distillate. Conclusion The redistilled cow＇s urine distillate posseses strong anfigenotoxic and antielastogenic properties against HPNLs and HLC treated with Cr^＋6 and MnO2. This property is mainly due to the antioxidants present in RCUD.展开更多
目的建立不同剂量双酚S(Bisphenol S,BPS)暴露动物实验模型,分析大鼠尿液中BPS内暴露水平的动态变化。方法通过灌胃给药建立对照组、1μg/kg低剂量组、100μg/kg中剂量组和10 mg/kg高剂量组Sprague Dawley(SD)大鼠暴露模型,使用超高效...目的建立不同剂量双酚S(Bisphenol S,BPS)暴露动物实验模型,分析大鼠尿液中BPS内暴露水平的动态变化。方法通过灌胃给药建立对照组、1μg/kg低剂量组、100μg/kg中剂量组和10 mg/kg高剂量组Sprague Dawley(SD)大鼠暴露模型,使用超高效液相色谱-串联质谱法(Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry,UPLC-MS/MS)内标法检测大鼠在连续6周暴露不同剂量BPS后其尿样中BPS含量的变化。结果目标物BPS在0.2~100μg/L的范围内呈良好线性关系,r^(2)≥0.998,方法检出限为0.01μg/L,定量限为0.03μg/L。对大鼠尿液的检测结果表明,随着暴露时间的增加,BPS在各剂量组大鼠体内的生物累积效应明显,尿液中BPS的内暴露水平在给药的6周中持续升高,且从给药后的第一周开始,尿液BPS含量的组间差异均有统计学意义(P<0.05)。结论本研究成功建立了不同剂量BPS暴露的SD大鼠动物实验模型,准确测定了大鼠连续6周尿液中双酚S的内暴露水平,为BPS毒理学剂量-效应关系研究提供参考。展开更多
Objective To study immunologic mechanism of tiger＇ s urine on patients of rheumatoid arthritis.Method We used ELISA method to detect TNF values of serum;we also used MTT colorimetric method to detect the inhibition o...Objective To study immunologic mechanism of tiger＇ s urine on patients of rheumatoid arthritis.Method We used ELISA method to detect TNF values of serum;we also used MTT colorimetric method to detect the inhibition of tiger＇ s urine on T -cell.Result TNF values of RA patient ＇ s serums at both active stage and stat ionary phase before the therapy were higher than those of healthy group;T NF values of patient ＇ s serums after the therapy were lower than those before the therapy;tiger＇ s urine had obvious inhibitive actio n on proliferation of peripheral blood＇ s T -cell.Conclusion Tiger＇ s urine could inhibit T -cell＇ s proliferation and TNF＇ s secretion,which may be the im-portant mechanism of curing RA.展开更多
BACKGROUND Due to new restrictions on the use of bisphenol A(BPA),industries are beginning to replace it with derived molecules such as bisphenol S and F(BPS and BPF).There is extensive evidence in the academic litera...BACKGROUND Due to new restrictions on the use of bisphenol A(BPA),industries are beginning to replace it with derived molecules such as bisphenol S and F(BPS and BPF).There is extensive evidence in the academic literature on the potential health effects of BPA,which is known to be a diabetogenic molecule.However,there are few publications related to new compounds derived from BPA.AIM To perform an epidemiological study of urinary BPS and BPF in the American National Health and Nutrition Examination Survey(NHANES)cohort,and analyze their possible relationship with diabetes mellitus.METHODS NHANES datasets from 2013 to 2016 were used due to the urinary BPF and BPS availability.Data from 3658 adults were analyzed to perform regression analysis exploring the possible relationship between BPA-derived compounds and diabetes.RESULTS Descriptive statistics,linear regression modeling,and logistic regression analysis revealed a significant relationship between urinary BPS,but not BPF,and diabetes risk.Additionally,a relationship was observed between both compounds and hypertension and a slight relationship between BPF and dyslipidemia.CONCLUSION In the present study,a strong relationship between urinary BPS,not BPF,and diabetes risk has been determined.BPA substitute molecules do not exempt the population from potential health risks.展开更多
以取自四川省不同地区的牦牛粪便、肠道内容物为材料,用MRS琼脂双层培养基进行厌氧培养,分离到50株乳酸菌,经生化鉴定为嗜热链球菌(2株)、乳酸乳球菌(1株)、保加利亚乳杆菌(5株)、嗜粪乳杆菌(10株)、嗜酸乳杆菌(8株)、乳酸乳杆菌(9株)...以取自四川省不同地区的牦牛粪便、肠道内容物为材料,用MRS琼脂双层培养基进行厌氧培养,分离到50株乳酸菌,经生化鉴定为嗜热链球菌(2株)、乳酸乳球菌(1株)、保加利亚乳杆菌(5株)、嗜粪乳杆菌(10株)、嗜酸乳杆菌(8株)、乳酸乳杆菌(9株)、肠乳杆菌(10株)、弯曲乳杆菌(5株)。采用乳酸菌16 S rDNA通用引物,对分离的8种菌的16 S rDNA一段可变区序列进行扩增,均得到大小约470 bp的产物;扩增产物经纯化、测序后与GenBank中标准菌株的核甘酸序列比较,同源性均大于97．5％,同源性分析与生化试验的结果是一致的。证实,牦牛肠道和粪便的乳酸菌较为丰富,且乳杆菌的数量较多,这可能与牦牛复杂的生长环境有关。展开更多
文摘Due to limited resources and experience, rapid diagnostic techniques are advocated in nations with a resource shortage when diagnosing schistosomiasis. We used rapid diagnostic tests to access the prevalence and intensity of schistosome infection in North Central, Nigeria. A total of 1951 participants were recruited for this study. The participants were screened for S. haematobium infection;haematuria and proteinuria were monitored in the recruited patients with a commercial reagent strip. Of the 1951 participants recruited for the study, 587 were found to be infected. Children aged 0 to 10 years showed the highest levels of haematuria with (100%) specificity. Meanwhile, other age groups (11 - 20, 21 - 30, 31 - 40 and above 40 years) had rates higher than 90%. The degree of haematuria increased with egg intensity. The same was seen in proteinuria, with a percentage of 41.9%. A significant difference (p S. haematobium in rural endemic areas.
文摘Objective To study the anticlastogenic effect of redistilled cow＇s urine distillate （RCUD） in human peripheral lymphocytes （HLC） challenged with manganese dioxide and hexavalent chromium. Methods The anticlastogenic activity of redistilled cow＇s urine distillate was studied in human polymorphonuclear leukocytes （HPNLs） and human peripheral lymphocytes in vitro challenged with manganese dioxide and hexavalent chromium as established genotoxicants and clastogens which could cause induction of DNA strand break, chromosomal aberration and micronucleus. Three different levels of RCUD： 1 μL/mL, 50 μL/mL and 100 μL/mL, were used in the study. Results Manganese dioxide and hexavalent chromium caused statistically significant DNA strand break, chromosomal aberration and micronucleus formation, which could be protected by redisfilled cow＇s urine distillate. Conclusion The redistilled cow＇s urine distillate posseses strong anfigenotoxic and antielastogenic properties against HPNLs and HLC treated with Cr^＋6 and MnO2. This property is mainly due to the antioxidants present in RCUD.
文摘目的建立不同剂量双酚S(Bisphenol S,BPS)暴露动物实验模型,分析大鼠尿液中BPS内暴露水平的动态变化。方法通过灌胃给药建立对照组、1μg/kg低剂量组、100μg/kg中剂量组和10 mg/kg高剂量组Sprague Dawley(SD)大鼠暴露模型,使用超高效液相色谱-串联质谱法(Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry,UPLC-MS/MS)内标法检测大鼠在连续6周暴露不同剂量BPS后其尿样中BPS含量的变化。结果目标物BPS在0.2~100μg/L的范围内呈良好线性关系,r^(2)≥0.998,方法检出限为0.01μg/L,定量限为0.03μg/L。对大鼠尿液的检测结果表明,随着暴露时间的增加,BPS在各剂量组大鼠体内的生物累积效应明显,尿液中BPS的内暴露水平在给药的6周中持续升高,且从给药后的第一周开始,尿液BPS含量的组间差异均有统计学意义(P<0.05)。结论本研究成功建立了不同剂量BPS暴露的SD大鼠动物实验模型,准确测定了大鼠连续6周尿液中双酚S的内暴露水平,为BPS毒理学剂量-效应关系研究提供参考。
文摘Objective To study immunologic mechanism of tiger＇ s urine on patients of rheumatoid arthritis.Method We used ELISA method to detect TNF values of serum;we also used MTT colorimetric method to detect the inhibition of tiger＇ s urine on T -cell.Result TNF values of RA patient ＇ s serums at both active stage and stat ionary phase before the therapy were higher than those of healthy group;T NF values of patient ＇ s serums after the therapy were lower than those before the therapy;tiger＇ s urine had obvious inhibitive actio n on proliferation of peripheral blood＇ s T -cell.Conclusion Tiger＇ s urine could inhibit T -cell＇ s proliferation and TNF＇ s secretion,which may be the im-portant mechanism of curing RA.
基金Supported by the Instituto de Salud Carlos III,No. PI15/02139Comunidad Autónoma de Madrid,No. B2017-BMD-3686
文摘BACKGROUND Due to new restrictions on the use of bisphenol A(BPA),industries are beginning to replace it with derived molecules such as bisphenol S and F(BPS and BPF).There is extensive evidence in the academic literature on the potential health effects of BPA,which is known to be a diabetogenic molecule.However,there are few publications related to new compounds derived from BPA.AIM To perform an epidemiological study of urinary BPS and BPF in the American National Health and Nutrition Examination Survey(NHANES)cohort,and analyze their possible relationship with diabetes mellitus.METHODS NHANES datasets from 2013 to 2016 were used due to the urinary BPF and BPS availability.Data from 3658 adults were analyzed to perform regression analysis exploring the possible relationship between BPA-derived compounds and diabetes.RESULTS Descriptive statistics,linear regression modeling,and logistic regression analysis revealed a significant relationship between urinary BPS,but not BPF,and diabetes risk.Additionally,a relationship was observed between both compounds and hypertension and a slight relationship between BPF and dyslipidemia.CONCLUSION In the present study,a strong relationship between urinary BPS,not BPF,and diabetes risk has been determined.BPA substitute molecules do not exempt the population from potential health risks.
文摘以取自四川省不同地区的牦牛粪便、肠道内容物为材料,用MRS琼脂双层培养基进行厌氧培养,分离到50株乳酸菌,经生化鉴定为嗜热链球菌(2株)、乳酸乳球菌(1株)、保加利亚乳杆菌(5株)、嗜粪乳杆菌(10株)、嗜酸乳杆菌(8株)、乳酸乳杆菌(9株)、肠乳杆菌(10株)、弯曲乳杆菌(5株)。采用乳酸菌16 S rDNA通用引物,对分离的8种菌的16 S rDNA一段可变区序列进行扩增,均得到大小约470 bp的产物;扩增产物经纯化、测序后与GenBank中标准菌株的核甘酸序列比较,同源性均大于97．5％,同源性分析与生化试验的结果是一致的。证实,牦牛肠道和粪便的乳酸菌较为丰富,且乳杆菌的数量较多,这可能与牦牛复杂的生长环境有关。
