MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

Gamma-aminobutyric acid(GABA)ergic neurons,the most abundant inhibitory neurons in the human brain,have been found to be reduced in many neurological disorders,including Alzheimer's disease and Alzheimer's disease-related dementia.Our previous study identified the upregulation of microRNA-502-3p(miR-502-3p)and downregulation of GABA type A receptor subunitα-1 in Alzheimer's disease synapses.This study investigated a new molecular relationship between miR-502-3p and GABAergic synapse function.In vitro studies were perfo rmed using the mouse hippocampal neuronal cell line HT22 and miR-502-3p agomiRs and antagomiRs.In silico analysis identified multiple binding sites of miR-502-3p at GABA type A receptor subunitα-1 mRNA.Luciferase assay confirmed that miR-502-3p targets the GABA type A receptor subunitα-1 gene and suppresses the luciferase activity.Furthermore,quantitative reve rse transcription-polymerase chain reaction,miRNA in situ hybridization,immunoblotting,and immunostaining analysis confirmed that overexpression of miR-502-3p reduced the GABA type A receptor subunitα-1 level,while suppression of miR-502-3p increased the level of GABA type A receptor subunitα-1 protein.Notably,as a result of the overexpression of miR-502-3p,cell viability was found to be reduced,and the population of necrotic cells was found to be increased.The whole cell patch-clamp analysis of human-GABA receptor A-α1/β3/γ2L human embryonic kidney(HEK)recombinant cell line also showed that overexpression of miR-502-3p reduced the GABA current and overall GABA function,suggesting a negative correlation between miR-502-3p levels and GABAergic synapse function.Additionally,the levels of proteins associated with Alzheimer s disease were high with miR-502-3p overexpression and reduced with miR-502-3p suppression.The present study provides insight into the molecular mechanism of regulation of GABAergic synapses by miR-502-3p.We propose that micro-RNA,in particular miR-502-3p,could be a potential therapeutic to rget to modulate GABAergic synapse function in neurological disorders,including Alzheimer's disease and Alzheimer's diseaserelated dementia.

Hybrid Ⅲ-50th-RS假人上腹部冲击试验探究

列车碰撞事故中,人体腹部是一个比较容易受到严重损伤的部位之一,为开发设计出适用于列车碰撞试验所用假人——Hybrid Ⅲ-50th-RS假人的腹部,开展针对假人上腹部的冲击试验探究。Hybrid Ⅲ-50th-RS假人上腹部的研究主要是参考THOR-NT假人上腹部冲击试验标准,结合国外Hybrid Ⅲ-50th-RS上腹部标定方法进行的试验探索。利用假人生产制作的常用材料,研究假人上腹部多层泡沫材料的组合方案、假人坐姿、压力分配板与腹袋是否连接、是否穿胸部皮肤以及假人上腹部的结构等对假人上腹部标定的影响及其响应特性。通过对试验数据进行分析,总结Hybrid Ⅲ-50th-RS假人上腹部冲击标定的影响因素及试验规律。根据所参考的THOR-NT假人上腹部冲击试验生物力学响应标准,确定符合试验要求的Hybrid Ⅲ-50th-RS假人上腹部的材料组合及其结构方案。研究结果表明:进行Hybrid Ⅲ-50th-RS假人上腹部冲击试验时,假人配有胸部皮肤,保持上腹部水平,压力分配板与腹袋保持连接更符合试验要求。采用后层2块丁晴橡胶板+中层三元乙丙泡沫+前层聚氨酯泡沫的“四层式”腹部结构和“前软中软”的材料搭配方案,具有较好的生物力学响应特性,适合用做列车碰撞试验假人上腹部。

Chromosome analysis of esophageal squamous cell carcinoma cell line KYSE 410-4 by repetitive multicolor fluorescence in situ hybridization

Chromosome aberrations are distinctive features of human malignant tumors. Analysis of chromosomal changes can illuminate the molecular mechanisms underlying the development and progression of cancer. To establish the technique of multicolor fluorescence in situ hybridization （M-FISH） for identifying chromosome aberrations in esophageal carcinoma cell line KYSE 410-4, four pools of 6-color whole-chromosome painting probes have been designed and hybridized on the same metaphase spread by four rounds of repetitive FISH. Repetitive 6-color M-FISH was successfully established and the cytogenetic abnormalities in KYSE 410-4 cells were characterized. Chromosome gains occurred at 2q, 3, 8, 17p, and X. An isochromosome 3q was visualized in the cell line, which might be one intermediate mechanism leading to 3p losses and/or 3q gains. Furthermore, 16 structural arrangements were detected, including four derivative chromosomes. The rearrangement of the centromeric regions accounted for approximately 44% of all rearrangements. The results added a more complete and accurate information of the genetic alterations to the classical cytogenetic description of KYSE 410-4 and provided a detailed cytogenetic background data for appropriate use of the cell line. The established 6-color M-FISH was useful for analyzing chromosomes in the whole genome of human tumors.

A hybrid Lagrangian-Eulerian numerical model for sea-ice dynamics

A hybrid Lagrangian - Eulerian （HLE） method is developed for sea ice dynamics, which combines the high computational efficiency of finite difference method （FDM） with the high numerical accuracy of smoothed particle hydrodynamics （SPH）. In this HLE model, the sea ice cover is represented by a group of Lagrangian ice particles with their own thicknesses and concentrations. These ice variables are interpolated to the Eularian gird nodes using the Gaussian interpolation function. The FDM is used to determine the ice velocities at Eulerian grid nodes, and the velocities of Lagrangian ice particles are interpolated from these grid velocities with the Gaussian function also. The thicknesses and concentrations of ice particles are determined based on their new locations. With the HLE numerical model, the ice ridging process in a rectangular basin is simulated, and the simulated results are validated with the analytical solution. This method is also applied to the simulation of sea ice dynamics in a vortex wind field. At last, this HLE model is applied to the Bohai Sea, and the simulated concentration, thickness and velocity match the satellite images and the field observed data well.

Multiple-stacked Hybrid Plays of lacustrine source rock intervals：Case studies from lacustrine basins in China

Hydrocarbon-producing lacustrine basins are widely developed in the world, and China has a large number of lacustrine basins that have developed since the early Permian. The organic-rich shale-dominated heterogeneous source rock intervals in Chinese lacustrine basins generally contain frequent thin interbeds of stratigraphically associated sandstone, siltstone, marl, dolomite, and limestone. The concept of ‘‘Hybrid Plays＇ ＇ as put forth in this article recognizes this pattern of alternating organicrich shale and organic-lean interbeds and existence of mixed unconventional and conventional plays. Hybrid Plays in lacustrine source rock intervals present a unique closed petroleum system hosting continuous hydrocarbons.The interbedded organic-lean siliciclastic and/or carbonateplays are efficiently charged with hydrocarbons via short migration pathways from the adjacent organic-rich shale that is often also a self-sourced play. We assert ‘‘Hybrid Plays＇ ＇ provide the most realistic exploration model for targeting multiple-stacked and genetically related very tight shale, tight and conventional plays together in the entire source rock interval rather than individual plays only. The Hybrid Play model has been proven and works for a wide variety of lacustrine rift, sag and foreland basins in China.

S波段HYBRID聚束-加速结构的样机研制

Hybrid聚束-加速结构是把驻波预聚束器、行波聚束器和标准加速管集成到一起的新型RF结构。简述了对S波段Hybrid聚束-加速结构样机的束流动力学优化和微波设计结果,解释了Hybrid结构导致发射度增长的原因,对此样机进行了射频低功率测试。样机的冷测结果与RF设计结果一致性很好。在冷测频率2855.21 MHz处,实测S11小于-45 dB,腔间相移偏差小于±2°,VSWR≤1.2对应的带宽大于5 MHz,轴向场分布完全满足动力学要求。

Synthesis and Characterization of Polystyrene/Nanosilica Organic-Inorganic Hybrid

A polystyrene（PS）/nanosilica organic-inorganic hybrid material was prepared from styrene monomer and commercial aqueous silica sol containing large amounts of Si-OH by means of emulsion polymerization. The nanosilica sol was modified by the addition of the reactive coupling agent methacrylexy propyltrimethoxysilane （ MPS）, and the resulting latex particles were protected by surfactants such as sodium dodecyl sulphonate（ SDS）, hydroxypropyl methyl cellulose （ HMPC）, and poly （vinylpyrrolidone） （PVP）. The effects of the type of surfactant, the amount of surfactant, and the coupling agent on the shape and stability of the resulting latex particles were investigated. The TEM observation indicates that among SDS, HMPC, and PVP, SDS is the best surfactant. When the content of SDS is 0. 5% and the amount of MPS is 7% in the system, the latex with obvious core-shell structure could be obtained. The average diameters of the monodispersed particles range from 182 to 278 nm, and the average number of silica beads for each composite are 1325 and 4409, respectively. The FrIR analysis shows that PS was chemically linked to silica through MPS. The thermal gravimetric analysis shows that when there is a higher silica content, the hybrid composites have a better heat resistance.

SYNTHESIS AND CHARACTERIZATION OF STRUCTURALLY WELL-DEFINED POLYMER-INORGANIC HYBRID NANOPARTICLES VIA ATRP

Atom transfer radical polymerization (ATRP) using cuprous chloride/2,2'-bipyridine (bipy) was applied to graft polymerization of styrene on the surface of silica nanoparticles to synthesize polymer-inorganic hybrid nanoparticles, 2-(4Chloromethylphenyl) ethyltriethoxysilane (CTES) was immobilized on the surface of silica nanoparticles through condensation reaction of the silanol groups on silica with triethoxysilane group of CTES. Then ATRP of St was initiated by this surface-modified silica nanoparticles bearing benzyl chloride groups, and formed PSt graft chains on the surface of silica nanoparticles. The thickness of the graft chains increased with reaction time. End group analysis confirmed the occurrence of ATRP. Thermal analysis indicated that thermal stabilization of these resulting hybrid nanoparticles also increases with polymerization conversion. The results above show that this 'grafting from' reaction could be used for the preparation of polymer-inorganic hybrid nanoparticles with controlled structure of the polymer's end groups.

Predicting quantitative structure-activity relationship of substituted 17α-acetoxyprogesterones by molecular hybridization electronegativity-distance vector

A set of novel structural descriptors (molecular hybridization electronegativity-distance vector, VMEDh) was put forward, and the quantitative structure–activity relationship (QSAR) of a series of 17α-Acetoxyprogesterones (APs) was investigated. Taking into account the effect of various hybridized orbits on atomic electronegativities, we developed the structure descriptors with amended electronegativities to build a QSAR model. The 10-parameter model based on VMEDh yields a correlation coefficient R=0.972 and standard deviation SD=0.262, which are more desirable than those of the previous molecular electonegativity-distance vector (MEDV-4) (R=0.969, SD=0.275). By stepwise multiple linear regression, several parameters are selected to construct optimal models. The 7-parameter model based on VMEDh has R=0.960 and SD=0.276; its correlation coefficient (RCV) and standard deviation (SDCV) for leave-one-out procedure crossvalidation are respectively RCV=0.890 and SDCV=0.445. The 6-parameter MEDV-4 model has R=0.946, SD=0.304, RCV=0.903 and SDCV=0.406. It is demonstrated that VMEDh has desirable estimation performance and good predictive capability for this series of chemical compounds.

HybridⅢ-50th假人颈部标定试验的影响因素研究

假人标定试验的目的是检验其是否满足性能要求,直接决定了汽车碰撞试验结果的准确性。为提高假人颈部标定试验的效率,文章从摆锤的撞击速度、蜂窝铝的数量2个方面入手,采用控制变量法设计了7组HybridⅢ-50th假人颈部标定试验,分析其对颈部标定结果的影响。结果表明:摆锤的撞击速度对D平面的最大转角和枕骨处最大力矩影响显著;蜂窝铝的数量对枕骨处的最大力矩影响显著;二者皆对摆锤的减速度有一定影响。

Formation of Hybrid Ring Structure of Cyanurate/Isocyanurate in the Reaction be-tween 2,4,6-Tris(4-Phenyl-Phenoxy)-1, 3,5-Triazine and Phenyl Glycidyl Ether

Reaction products of 2,4,6-tris(4-phenyl-phenoxy)-1,3,5-triazine derived from 4-phenylphenol cyanate ester and phenyl glycidyl ether were analyzed. In addition to an isocyanurate compound and an oxazolidone compound which were well known as reaction products of cyanate esters and epoxy resins, compounds with hybrid ring structure of cyanurate/isocyanurate were determined. Gibbs free energies of the compound having hybrid ring structure of cyanurate/isocyanurate with two isocyanurate moiety were found to be lower than that of the compound with cyanurate ring structure through calculations. Calculation data supported the existence of hybrid ring structure of cy-anurate/isocyanurate. It was revealed that isomerization from cyanurate to isocyanurate occurs via hybrid ring structure of cyanurate/isocyanurate in the reaction of aryl cyanurate and epoxy.

Breeding and Cultivation Techniques of Edible Sunflower Variety AR9-9293

Edible sunflower variety AR9-9293 was approved by the Sunflower Variety Appraisal Committee with the country Identification number of sunflower 2015005. This paper introduced the breeding process, yield performance and characteristics, and the high-yield cultivation technology of new edible sunflower variety AR9-9293. The promotion of edible sunflower varieties and cultivation technology could achieve the effects of improving the level of the sunflower cultivation, promoting the development of rural and agricultural economy and increasing farmers＇ income, as well as positively promoting development of the society and adjusting agricultural industry structure.

非线性差分-微分方程的Jacobi椭圆函数解

基于椭圆函数展开法和tanh函数法,引入构造非线性离散系统行波解的方法,并给出了离散mKdVlattice方程和(2+1)-维Hybrid lattice方程的一些新的椭圆函数解.

Expression of Angiopoietin-1/-2 in the Process of Mouse Embryo Implantation

This study examined the expression and distribution of angiopoietin-1/-2 （Ang-1/-2） in the endometrium of early pregnant mice. The expression of Ang-1/-2 was detected by immunohistochemical staining and in situ hybridization respectively. Computerized image analysis system was used to measure the average optical intensity of Ang-1/-2 in endometria at different time points after gestation. Mice were randomly divided into 5 groups： control group, D2 group （2 days after pregnancy）, D4 group （4 days after pregnancy）, D6 group （6 days after pregnancy） and D8 group （8 days after pregnancy）, each containing 15 mice. The results showed that the expression of Ang-1 and Ang-2 was very different among 4 groups （P〈0.01）. Immunohistochemical staining revealed that Ang-1 was localized in the cytoplasma of stromal cells 2 days after pregnancy （day 2）, and in luminal epithelial cells on day 4. The protein of Ang-2 was mainly expressed in the cytoplasma of glandular epithelia and stromal cells. With gestation time, the positive reactions of Ang-1/-2 were stronger in the endometria of the pregnant mice （P〈0.01）. In situ hybridization showed Ang-I mRNA in stromal cells on day 2. Hybridization signal was localized in both stromal cells and vessel epithelial cells on day 4; Ang-2 mRNA was expressed in stromal cells and glandular epithelia on day 2; high mRNA levels appeared in stromal cells, glandular epithelia and vascular endothelia on day 4; an increasing in mRNA expression of Ang-1/-2 was observed on day 6 and day 8 （P〈0.01）. It is suggested that Ang-1/-2 may play an important role in the cross-talk between blastocyst and maternal endometrium during the process of embryo implantation.

Improved delayed detached-eddy simulation of massive separation around triple cylinders

The massively separated flow past triple cylin- ders （TriC） in tandem arrangement is simulated using the improved delayed detached-eddy simulation （IDDES） method based on the shear stress transport （SST） model, coupled with the high order adaptive dissipation scheme. The spacing between adjacent cylinders is sub-critical （1.435D）. IDDES prediction of two cylinders （TC） with the same spacing is compared to experimental data for validation, and the numerical results agree well with the available measurements, except for the asymmetry in the gap region. The flow past TriC is investigated using the same method. Generally, the mean flow quantities past TriC, such as the velocity, pressure, and vorticity, are similar to the corresponding components of TC. However, the pressure fluctuations on the TriC surface are uniformly larger than those on TC. Meanwhile, the instantaneous flows past TriC are much more complex. The periodical blockage in the first gap region is found in the TriC case and leads to the up-and-down movement of shear layer in the second gap region.

应用hybrid术式治疗多节段脊髓型颈椎病的疗效观察

目的探讨hybrid术式治疗多节段脊髓型颈椎病的疗效。方法 2011-04~2013-05对该科收治的多节段颈椎病患者37例行颈椎前路融合与非融合相结合的hybrid术式(A组),记录手术前后JOA、NDI评分、颈椎总活动度及手术节段的邻近节段的活动度,术后随访2.5年,通过与单纯融合组(B组)比较,分析其治疗效果。结果术后2.5年A、B两组患者JOA评分及改良率、NDI评分、轴性症状的构成比、颈椎总活动度、手术前后邻近节段活动度差值相比,差异有统计学意义(P<0.05)。结论 hybrid术式能够保留一定节段的活动度,避免邻近节段的过度代偿导致的应力负荷增加,是治疗多个节段脊髓型颈椎病的有效方法。

Dynamics of cantilever plates and its hybrid vibration control

Applying Lagrange-Germain＇s theory of elas- tic thin plates and Hamiltonian formulation, the dynamics of cantilever plates and the problem of its vibration control are studied, and a general solution is finally given. Based on Hamiltonian and Lagrangian density function, we can obtain the flexural wave equation of the plate and the relationship between the transverse and the longitudinal eigenvalues. Based on eigenfunction expansion, dispersion equations of propagation mode of cantilever plates are deduced. By satisfying the boundary conditions of cantilever plates, the natural frequencies of the cantilever plate structure can be given. Then, analytic solution of the problem in plate structure is obtained. An hybrid wave/mode control approach, which is based on both independent modal space control and wave control methods, is described and adopted to analyze the active vibration control of cantilever plates. The low-order （controlled by modal control） and the high-order （controlled by wave control） frequency response of plates are both improved. The control spillover is avoided and the robustness of the system is also improved. Finally, simulation results are analyzed and discussed.

Axial variations in anatomical properties and basic density of Eucalypt urograndis hybrid(Eucalyptus grandis × E. urophylla)clones

We studied two clones of Eucalypt urograndis hybrid （Eucalyptus grandis × E. urophylla）, GR283 and GR330, grown in Tumkur district of Kamataka （India）, and felled 5-6 years old three trees of each clone. We recorded axial variations in heartwood content, bark properties, wood density and anatomical characteristics of wood in- cluding fibre length, fibre diameter, fibre wall thickness, lumen diameter, vessel frequency, vessel diameter and vessel element length. Clone GR283 had about 10 % heartwood, significantly lower than for clone GR330 （37 %）. Basic wood density along the tree height varied significantly within and between the clones. We observed significant variations in fibre length, fibre diameter and wall thickness within and between the two clones. Vessel frequency and vessel element length did not vary but vessel diameter differed significantly between the clones. With a greater proportion of sapwood, clone GR283 can be uti- lized for paper and pulp applications. Clone GR330 had a higher proportion of heartwood and lower wood density and, hence, is more suitable for light-weight material applications.

e动绿色生活 亲历一汽-大众奥迪2011科技日(下)

高效．节能的“千里宝驹” 整个试驾安排中，唯一能驶上英戈尔斯塔特道路长距离体验的，便是奥迪Q5 hybrid quattro。全混合动力车。这款车首先将于今年下半年在欧洲上市，明年将进入中国市场，因此，它将成为奥迪e-tron家族中离我们生活最近的新能源车型。

一汽-大众奥迪车 主说服务

在刚刚结束的第11届北京国际车展期间，一汽-大众奥迪品牌携旗下21款车型亮相本届车展。其中，全球首发的全新旗舰轿车奥迪A8L，携手顶级SUV Q7 exclusive concept V12和超级跑车R8 Spyder一起组成了强大的旗舰车型阵容。此外，亚洲首发的全新A8 hybrid以及两款纯电动车型——四驱版R8 e-tron以及A1 e—tron，也首次向中国观众展示了奥迪在新能源汽车方面的多样化解决方案。