核转录因子Yin-Yang1与中医阴阳学说

Yin- Yang 1 ( YY1 )是一种具有双重转录活性的核转录因子 ,广泛存在于真核细胞中 ,自被发现十余年来 ,国外对其研究报道逐年增多 ,对其在基因转录调控、分子信号转导中所表现出的关键作用的认识日益引起重视。本文综述了近年来 YY1研究的部分相关文献 ,分析了 YY1与中医阴阳的关系 ,以期为中医阴阳学说的现代研究带来新的思路。

Cold environments and human metabolism: A traditional chinese medicine perspective

The concept of Traditional Chinese Medicine(TCM)emphasizes the intrinsic connection between human beings and nature,positing that the human body undergoes distinct physiological changes in response to various natural environments.Cold,as a primary external factor in cold areas,necessitates the body's autonomous adaptation to uphold optimal living conditions.The repercussions of cold on the body are both far-reaching and profound,with metabolic equilibrium adjustments playing a pivotal role.This article,rooted in the TCM principle of Yin-Yang balance,delves into the metabolic intricacies and adaptive responses to the human body in cold environments.The effects manifest in heat-producing tissues,systemic substance consumption,the blood substance concentrations,liver function,and metabolic rhythms.The article subsequently presents TCM recommendations for maintaining health in cold climates.It concludes by advocating the exploration of metabolic homeostasis changes as a key avenue for investigating the metabolic traits s of populations in cold regions.We posit that such insights will enhance comprehension of the metabolic shifts in cold region populations and advance the evolution of regional medicine.

On the Possible Structures of Electron and Proton

In this work, the possible structures of electron and proton have been explored. Based on the potential expressions of electron and proton, we found that the electron and proton share the similar structure inside re and rn. And within re and rn, the conventional charge concept stops working, the same charge repelling force doesn’t exist anymore and as a result, the requirement of charge conservation is automatically removed. Whereas beyond re and rn, the potential expressions of electron and proton obey the point charge potentials as we normally understand. Therefore, the conventional charge concept can be applied and the requirement of charge conservation takes effect. Furthermore, a possible mechanism for the creations of electric monopole and magnetic monopole is discussed. In addition, to compare the particle size in micro-world, the balloon criterion is proposed. By this balloon criterion, the proton is determined about 10 times bigger than electron. From the physical picture about electron and proton described above, the stabilities of electron and proton can be explained quite well.

An Improved Dynamic Core for a Non-hydrostatic Model System on the Yin-Yang Grid

A 3D dynamic core of the non-hydrostatic model GRAPES（Global/Regional Assimilation and Prediction System） is developed on the Yin-Yang grid to address the polar problem and to enhance the computational efficiency. Three-dimensional Coriolis forcing is introduced to the new core, and full representation of the Coriolis forcing makes it straightforward to share code between the Yin and Yang subdomains. Similar to that in the original GRAPES model, a semi-implicit semi-Lagrangian scheme is adopted for temporal integration and advection with additional arrangement for cross-boundary transport. Under a non-centered second-order temporal and spatial discretization, the dry nonhydrostatic frame is summarized as the solution of an elliptical problem. The resulting Helmholtz equation is solved with the Generalized Conjugate Residual solver in cooperation with the classic Schwarz method. Even though the coefficients of the equation are quite different from those in the original model, the computational procedure of the new core is just the same. The bi-cubic Lagrangian interpolation serves to provide Dirichlet-type boundary conditions with data transfer between the subdomains. The dry core is evaluated with several benchmark test cases, and all the tests display reasonable numerical stability and computing performance. Persistency of the balanced flow and development of both the mountain-induced Rossby wave and Rossby–Haurwitz wave confirms the appropriate installation of the 3D Coriolis terms in the semi-implicit semi-Lagrangian dynamic core on the Yin-Yang grid.

UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine

Cyclic 3’,5’-adenosine monophosphate(cAMP)and cyclic 3’,5’-guanosine monophosphate(cGMP)are considered as potential biomarkers for Yin-Yang disharmony in traditional Chinese medicine.However,phosphodiesterase-mediated ex vivo degradation of these molecules in biological samples may result in their underestimation.In the present study,a ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was developed for determination of cAMP and cGMP in rat plasma,with special consideration of their stability ex vivo.Following precipitation of proteins from plasma samples with 0.4 M perchloric acid,the analytes were chromatographed on a Shimadzu Shimpack-XR-ODS II column with 2.5 mM ammonium acetate and methanol in gradient mode.The MS/MS detection was performed using multiple reaction monitoring in the positive electrospray ionization mode.The lower limit of quantification was 0.27 ng/mL for cAMP and 0.37 ng/mL for cGMP.The method was used to determine the plasma cAMP and cGMP levels in normal and Yin deficiency diabetic rats treated with or without Rehmannia glutinosa.The developed method may be useful for evaluating the regulatory effects of Chinese herbal medicine on the levels of cAMP and cGMP in the body.

Influence of TBX21 T-1993C variant on autoimmune hepatitis development by Yin-Yang 1 binding

AIM To investigated the mechanism of the association between the TBX21 T-1993 C promoter polymorphism and autoimmune hepatitis type 1(AIH-1) development.METHODS In vivo,In vivo,and reporter analyses were performed to determine the function of transcription factors binding to the T-1993 C element of the TBX21 promoter in human CD4+ T and B cell lines. Flow cytometry and quantitative real-time PCR were used to analyze T-box transcription factor(T-bet) and interferon-γ(IFN-γ) expressions in CD4+ T cells,B cells and monocytes from the peripheral blood of AIH-1 patients including 5-1993 TC and 15-1993 TT genotype carriers,and healthy controls including 10-1993 TC and 25-1993 TT genotype carriers. Furthermore,a range of biochemical indices was measured simultaneously in the blood of AIH-1 patients.RESULTS TBX21-1993 C allele created a strong Yin-Yang 1(YY1)-binding site and decreased transcriptional activity of TBX21 promoter in human CD4+ T and B cells.Higher levels of T-bet and IFN-γ were detected in the circulating CD4+ T cells and B cells of AIH-1 patients carrying the TBX21-1993 TT genotype compared with the patients carrying the-1993 TC genotype and controls with the-1993 TC genotype. T-bet expression levels of circulating T cells and B cells were positively correlated with AIH-1 disease activity. Knockdown of YY1 with si RNA caused increased expression of T-bet and IFN-γ in peripheral blood mononuclear cells in AIH-1 patients. CONCLUSION The repression of TBX21 expression by high-affinity binding of YY1 to the-1993 C allele may contribute to a decreased development of AIH-1 via suppression of type 1 immunity.

An Accurate Multimoment Constrained Finite Volume Transport Model on Yin-Yang Grids

A global transport model is proposed in which a multimoment constrained finite volume （MCV） scheme is applied to a Yin-Yang overset grid. The MCV scheme defines 16 degrees of freedom （DOFs） within each element to build a 2D cubic reconstruction polynomial. The time evolution equations for DOFs are derived from constraint conditions on moments of line-integrated averages （LIA）, point values （PV）, and values of first-order derivatives （DV）. The Yin-Yang grid eliminates polar singularities and results in a quasi-uniform mesh. A limiting projection is designed to remove nonphysical oscillations around discontinuities. Our model was tested against widely used benchmarks; the competitive results reveal that the model is accurate and promising for developing general circulation models.

Implementation of CMA-GFS 3D-Var System on the Yin-Yang Grid

The global three-dimensional variational(3D-Var)data assimilation is implemented on a new quasi-uniform overset(Yin-Yang)grid on sphere.As a quasi-uniform spherical grid,it covers the sphere by overlapping two perpendicularly oriented grid components which is nothing but low latitude region of the usual latitude-longitude grid.Based on this characteristic of the Yin-Yang grid,it enables us to implement the regional 3D-Var system efficiently and accurately on the Yin or Yang component grid,respectively.The global analysis could update directly from the regional analysis since they have the same configurations like the precondition of eigenvalue decomposition for vertical direction,recursive filtering for horizontal direction,minimization method and observation operator and so on.However,the balance equation and vector wind are needed to be paid more attention on the Yin grid analysis due to its coordinate transformation.How to spread the observation information near the boundary of Yin and Yang grid is a key to the 3D-Var analysis.Extending double the horizontal correlation length distance in the overset boundary of Yin and Yang grid has successfully solved the problem.The results show that the analysis on the Yin-Yang grid is reasonable and similar to the result on the latitude-longitude(LAT-LON)grid.This paper provides a promising strategy for the development of a 3D-Var global system for overset grids.

Women in Chinese Philosophy:Yin-Yang Theory in Feminism Constructing

The traditional way of thinking about women in ancient Chinese philosophy was grounded on the concepts of Yin and Yang.Yin and Yang are the basic principles of the being,the interrelation and interaction between which provide the original force for the emergence of all beings in the world.In the traditional family construction,men as Yang master the affairs outside the family,and women as Yin master the affairs inside the family(男主外,女主内),which means that women normally had no voice in the social affairs.Her field was limited inside the family.Furthermore,according to Confucian doctrine“husband guides wife”(夫为妻纲),husband is the decision maker of a family,as a king in a state.The prejudice against woman has persisted to the present.This paper will show that within the Confucian tradition,the ontological principles of Yin and Yang have been abused to a certain degree,and women became the sacrifice of the interpretation with this prejudice.The author will argue in this paper,that the Yin-yang principle itself is very flexible.It contains much richer possibilities of interpretation than that had been interpreted in the Confucian tradition.The author’s argument is that if we recognized that each person is a complete Yin-Yang system,we would understand that every human being is a complete and independent person with both virtues of Yin and Yang.Yin is not a substance which restricts women,neither Yang to men.From this perspective,every woman has the potentiality to reach her universality and individuality.The author’s claim is that in building feminism with the resource of ancient Chinese thought,the concepts of Yin and Yang can be revived and given a new life if we think about them in a functional way.In this framework,Yin and Yang are correlative functions in social and family operation.The properties of Yin and Yang are determined only in each particular affair.People practicing functions are Yin or Yang in temporality.

Clinical effect of Yin-Yang blending acupuncture combined with Banxia Baizhu Tianma Decoction in treatment of cervical vertigo

Objective:To explore the clinical effect of Yin-yang blending acupuncture with traditional Chinese medicine combined with Banxia Baizhu Tianma Decoction in treating patients with cervical vertigo.Methods:120 patients with cervical vertigo treated in our hospital were selected,and the acupuncture group and the acupuncture group were divided into 60 cases each according to the order of the patients.The two groups of patients were given basic treatment measures.Xia Baizhu Tianma Decoction was treated,and the control group was given Yin and Yang blending acupuncture.The left and right vertebral arteries(LVA),right vertebral arteries(RVA),and basal arteries(BA)were compared before and after treatment in the two groups.,Clinical symptom score,clinical efficacy.Results:after treatment,the Vm values of LVA,RVA,and BA in the acupuncture group were lower than those in the acupuncture group(P<0.05);after treatment,acupuncture The dizziness,neck and shoulder pain,headache,daily life work,psychological and social adaptability scores of the drug group were higher than those of the acupuncture group(P<0.05);after treatment,the cure rate of the acupuncture group was 42.37%,and the effective rate was 37.29%.The efficiency was 16.95%,the inefficiency was 3.39%,the cure rate in the acupuncture group was 26.32%,the marked efficiency was 35.09%,the effective rate was 29.82%,and the ineffective rate was 8.77%.The clinical efficacy of the acupuncture group was better than that of the acupuncture group(P<0.05).Conclusion:Traditional Chinese medicine Yin-yang reconciliation acupuncture combined with Pinellia Baizhu Tianma Decoction is definitely effective in treating patients with cervical vertigo,and it can effectively improve the clinical symptoms and clinical effects of patients.

The Dao of Mathematical Creativity,Symmetry Theories,and Yin-Yang Philosophy With Their Classroom Applications

This paper introduces creativity,one of the significant features in maths.The creative mathematical beauty of symmetry and asymmetry are worth investigating in the practicing of Taichi.Symmetrical variances make the real world.Symmetry can be regarded as a kind of mirroring,like the line symmetry,the mirror image of the other parts,or rotational symmetry.Such characteristics can be vividly demonstrated in the traditional Chinese philosophical yin-yang theory(YY).The accompanying of male and female reveals the friendly cooperative relationship.YY reflects the counterparts of vacancy and firm,inhale and exhale,noun and verb,theory and practice,light and heavy,slow and fast,the pace,the sound effect in the classroom.Such an analysis is undergone about yin and yang in Yi School and Dao De Ching(DDC).Furthermore,there are detailed arguments of the symmetric nature of every verse of DDC,revealing the cultural value of the language.Their applications in the class admit the recognition under the new liberal arts project by China’s Ministry of Education.Yet,the integration of research and teaching is required to go further.

The Chinese Yin-Yang Thinking in Antoni Tàpies’Artistic Creation

The writings and artistic works of Antoni Tàpies(2009;2011)mirror the influences of the cosmic vision of the Chinese yin-yang.Signs,numbers,and creative procedures related to this correlative thought prevail in his art.Tàpies integrated different iconological codes from the ancient Chinese thinking into his art to question the existential meaning of the universe and to express his thoughts on the cultural and socio-political controversies of his time.In other words,the images,media,and techniques that he utilized for his works were extensions of his life experiences intertwined with various socio-historical facts and his personal feelings.

Clinical observation on efficacy of compound of warming yang, descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with Yin-Yang deficiency and blood stasis syndrome

Objective:To observe the clinical efficacy of compound of owarming yang,descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome.Methods:Seventy-six patients of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome were randomly divided into observation group and control group,thirty-eight cases in each group.The control group was given conventional western medicine treatment,while the observation group took compound of owarming yang,descending turbidity and dredging collaterals orally on the basis of conventional western medicine treatment.The course of treatment covered for one month.Before and after treatment,we observed the scores of traditional Chinese medicine symptoms,indicators of renal function[serum creatinine(Scr),blood urea nitrogen(BUN),microalbuminuria(MALB)],indicators of glucose metabolism[fasting plasma glucose(FPG),2-hour postprandial blood glucose(2hPG),glycosylated hemoglobin(HbAlc)],indicators of hemorheology[plasma viscosity(PV),platelet aggregation rate(PAR),fibrinogen(FIB)],Cystatin-C(Cys-C),C-reactive protein(CRP)in the two groups.Results:After treatment,the clinical effect of the observation group was significantly better than the control group(P<0.05).The scores of traditional Chinese medicine symptoms,indicators of renal function(Scr、BUN、UAER),indicators of glucose metabolism(FPG、2hPG、HbAlc),indicators of hemorheology(PV、PAR、FIB),Cys-C and CRP in the two groups were decreased significantly compared with those before treatment(P<0.05),and the decrease in the observation group was superior to that in the control group(P<0.05).Conclusion:Compound of warming yang,descending turbidity and dredging collaterals has remarkable efficacy in treating of diabetic kidney disease patients with yin-yang deficiency and blood stasis syndrome by alleviating clinical symptoms,glucose metabolism,renal function and microcirculatory disturbance,and the mechanism related to alleviation of microinflammation.

Gender Relations in Kjell Askildsen’s A Lovely Place From the Perspective of Chinese Yin-Yang Theory

A Lovely Place(2019)is a collection of the Norwegian writer Kjell Askildsen’s short stories that is translated from German into Chinese by Yang Zhizi.It contains 18 short stories,whose male characters are mainly analyzed by researchers.The paper argues that female characters are playing an indispensable role in compensating the males’life stories and enlightening their worldviews according to Chinese Yin-Yang theory.The females can be classified into the following three groups in terms of their important relations to the males.Firstly,the female is the male’s companion in a certain part of the latter’s life.Secondly,the female facilitates the male’s intellectual growth either spontaneously or purposefully.Thirdly,the female struggles for independence to set an example for the male.The female characters in A Lovely Place present such femininity as beauty,energy,nostalgia,sentimentality,motherhood,etc.,stimulate the males to reflect on their own identities and fulfill their muscularity,and function as the genuine protagonists in the collection as far as the gender relations are concerned.

The First Documented Experience of Qi and an Account of Healing Failure:4th-Century BCE

This essay will review the earliest case that documents a patient’s experience of qi,one found on a bamboo text buried with the patient who died in the 318 BCE.Details of the healing encounter and of concepts of illness show how non-transmitted documents hidden from later editors in tombs preserve an older layer of medical understanding than that in transmitted canons,such as the Huang Di Nei Jing(Inner Canon of the Yellow Emperor).The 4th-century BCE case record described below is the longest early medical record concerning the treatment of a specific individual.It is also an account of failure formally recorded for the sake of the survivors and buried with the dead to be transmitted to the world of the spirits.The essay begins with a reevaluation of ancient concept of qi and then moves on to the individual case record.

“Yin-Yang philosophy”for the design of anticancer drug delivery nanoparticles

Understanding the in vivo transport process provides guidelines for designing ideal nanoparticles(NPs)with higher efficacy and fewer off-target effects.Many factors,such as particle size,morphology,surface potential,structural stability,and etc.,may influence the delivering process of NPs due to the existence of various physiological barriers within the body.Herein,we summarise the distinct influences of NP physicochemical properties on the four consecutive in vivo transport steps:(1)navigating with bloodstream within blood vessels,(2)transport across vasculature walls into tumour tissues,(3)intratumoural transport through the interstitial space,and(4)cellular uptake&intracellular delivery by cancerous cells.We found that the philosophy behind the current consensus for NP design has certain similarities to the“Yin-Yang”theory in traditional Chinese culture.Almost all physicochemical properties,regardless of big or small sizes,long or short length,positive or negative zeta potentials,are double-edged swords.The balance of potential benefits and side effects,drug selectivity and accessibility should be fully considered when optimising particle design,similar to the“Yin-Yang harmony”.This paper presents a comprehensive review of the advancements in NPs research,focusing on their distinct features in tumour targeting,drug delivery,and cell uptake.Additionally,it deliberates on future developmental trends and potential obstacles,thereby aiming to uncover the ways these characteristics influence the NPs’biological activity and provide theoretical guidance for the targeted delivery of NPs.

YY1基因在肺癌组织中的表达及其对肺癌细胞功能的影响

目的检测转录因子YY1在肺癌组织中的表达情况,并分析其对肺癌细胞的增殖、迁移及侵袭的影响。方法应用Taqman探针实时荧光定量PCR方法检测235例肺癌组织以及62例配对癌旁组织中YY1 mRNA的表达,并分析其表达与患者临床病理特征的关系。应用Lipofectamine 3000试剂在肺癌细胞系转染YY1过表达质粒及对照质粒。噻唑蓝(MTT)分别检测对照组和YY1过表达组在24、48及72 h时A549和H1299细胞增殖能力的变化。划痕实验和Transwell实验检测YY1过表达对肺癌细胞的迁移和侵袭能力的影响。结果与癌旁组织(5.80±0.58)相比,YY1 mRNA在肺癌组织(5.07±0.58)中表达下调(P<0.01),且YY1 mRNA的表达在不同性别、吸烟状态、病理类型及临床分期中差异有统计学意义(P<0.05)。YY1过表达组H1299细胞增殖能力在48 h和72 h均低于对照组(P<0.01),A549细胞在72 h低于对照组(P<0.05)。划痕实验和Transwell实验结果显示转染过表达YY1质粒后,肺癌细胞的侵袭能力及迁移能力明显下降(P<0.05)。结论 YY1在肺癌组织中低表达,上调YY1的表达可抑制肺癌细胞的增殖、迁移和侵袭。

Implementation of the Semi-Lagrangian Advection Scheme on a Quasi-Uniform Overset Grid on a Sphere

The semi-Lagrangian advection scheme is implemented on a new quasi-uniform overset （Yin-Yang） grid on the sphere. The Yin-Yang grid is a newly developed grid system in spherical geometry with two perpendicularly-oriented latitude-longitude grid components （called Yin and Yang respectively） that overlapp each other, and this effectively avoids the coordinate singularity and the grid convergence near the poles. In this overset grid, the way of transferring data between the Yin and Yang components is the key to maintaining the accuracy and robustness in numerical solutions. A numerical interpolation for boundary data exchange, which maintains the accuracy of the original advection scheme and is computationally efficient, is given in this paper. A standard test of the solid-body advection proposed by Williamson is carried out on the Yin-Yang grid. Numerical results show that the quasi-uniform Yin-Yang grid can get around the problems near the poles, and the numerical accuracy in the original semi-Lagrangian scheme is effectively maintained in the Yin-Yang grid.

Molecular Yin and Yang of erectile function and dysfunction

In regard to erectile function, Yin is flaccidity and Yang erection. In the past decade, research has mostly focused on the Yang aspect of erectile function. However, in recent years, the Yin side is attracting increasingly greater attention. This is due to the realization that penile flaccidity is no less important than penile erection and is actively maintained by mechanisms that play critical roles in certain types of erectile dysfunction （ED）; for example, in diabetic patients. In addition, there is evidence that the Yin and Yang signaling pathways interact with each other during the transition from flaccidity to erection, and vice versa. As such, it is important that we view erectile function from not only the Yang but also the Yin side. The purpose of this article is to review recent advances in the understanding of the molecular mechanisms that regulate the Yin and Yang of the penis. Emphasis is given to the Rho kinase signaling pathway that regulates the Yin, and to the cyclic nucleotide signaling pathway that regulates the Yang. Discussion is organized in such a way so as to follow the signaling cascade, that is, beginning with the extracellular signaling molecules （e.g., norepinephrin and nitric oxide） and their receptors, converging onto the intracellular effectors （e.g., Rho kinase and protein kinase G）, branching into secondary effectors, and finishing with contractile molecules and phosphodiesterases. Interactions between the Yin and Yang signaling pathways are discussed as well.

Two Definitions of Life Will Highlight on Physiological Understanding of Six Meridians

Treatise on Cold Pathogenic Diseases is an ancient guideline for traditional Chinese medicine(TCM) clinicians which classified all diseases into six meridians.In Treatise on Cold Pathogenic Diseases, many syndromes are enunciated in simple vocabularies without physiology and pathology, which is always criticized.It seems that a bridge is necessary for physiological and pathological understanding on Treatise on Cold Pathogenic Diseases.In past decade, two definition of life in cell and in our body are presented respectively.In both definitions, energy conversion is the core pathways for all organisms which can be regarded as motion of qi.Moreover, metabolism of energy agents can be seen as yang while gene expression and stem cell proliferation & differentiation(SCPD) should be seen as yin in cell and in our body respectively.The both definitions also outline the physiology and pathology of six meridians in Treatise on Cold Pathogenic Diseases, which can be unveiled as followings: taiyang meridian-elimination of energy and end metabolites;yangming meridian-acquiring energy agents;shaoyin meridian-the conversions of energy agents;taiyin meridian-new born somatic cell attending the metabolism of energy agents and then going to die;shaoyin meridian-storage and activity of stem cell;jueyin meridian-proliferation & differentiation of stem cell to produce somatic cell.These definitions will ignite new physiological and pathological understandings on Treatise on Cold Pathogenic Diseases.