The utilization of fish embryos in toxicity testing of hazardous chemicals has recently been adopted in order to satisfy stricter rules and regulations related to using adult animals in toxicity testing. This paper pr...The utilization of fish embryos in toxicity testing of hazardous chemicals has recently been adopted in order to satisfy stricter rules and regulations related to using adult animals in toxicity testing. This paper presents optimising steps towards improving zebra fish embryo production in the laboratory. Culture conditions were maintained in the aquaria as stipulated in the OECD draft proposal for a new guideline on fish embryo tests. Furthermore, a sequence of steps were adopted and followed to improve upon previous work done in the lab in 2006. About 200 eggs were produced in one spawn trap within an hour of onset of light, an improvement over the 50 - 60 eggs produced in the previous work. This result demonstrates that with the right culture conditions and proper optimisation of procedure the required number of embryos needed for toxicity testing can be obtained.展开更多
目的:检测饮用水和地表水源水的农药污染,为建立水质综合毒性的快速检测方法提供依据。方法:采用斑马鱼的急性毒性试验检测饮用水和地表水源水的毒性,用SPSS12.0统计分析计算斑马鱼的LC50。结果:对于0.2%的高渗甲维盐和25%毒死蜱氯氰菊...目的:检测饮用水和地表水源水的农药污染,为建立水质综合毒性的快速检测方法提供依据。方法:采用斑马鱼的急性毒性试验检测饮用水和地表水源水的毒性,用SPSS12.0统计分析计算斑马鱼的LC50。结果:对于0.2%的高渗甲维盐和25%毒死蜱氯氰菊酯乳液污染的饮用水和地表水,斑马鱼24 h LC50可以用来代替48 h和96 h LC50;在毒性测试过程中,斑马鱼对两种农药具有较高的灵敏性,最低可以达到微克。结论:斑马鱼的急性毒性测试结果可以用来快速检测饮用水和地表水源水农药污染的毒性。展开更多
文摘The utilization of fish embryos in toxicity testing of hazardous chemicals has recently been adopted in order to satisfy stricter rules and regulations related to using adult animals in toxicity testing. This paper presents optimising steps towards improving zebra fish embryo production in the laboratory. Culture conditions were maintained in the aquaria as stipulated in the OECD draft proposal for a new guideline on fish embryo tests. Furthermore, a sequence of steps were adopted and followed to improve upon previous work done in the lab in 2006. About 200 eggs were produced in one spawn trap within an hour of onset of light, an improvement over the 50 - 60 eggs produced in the previous work. This result demonstrates that with the right culture conditions and proper optimisation of procedure the required number of embryos needed for toxicity testing can be obtained.
文摘目的:检测饮用水和地表水源水的农药污染,为建立水质综合毒性的快速检测方法提供依据。方法:采用斑马鱼的急性毒性试验检测饮用水和地表水源水的毒性,用SPSS12.0统计分析计算斑马鱼的LC50。结果:对于0.2%的高渗甲维盐和25%毒死蜱氯氰菊酯乳液污染的饮用水和地表水,斑马鱼24 h LC50可以用来代替48 h和96 h LC50;在毒性测试过程中,斑马鱼对两种农药具有较高的灵敏性,最低可以达到微克。结论:斑马鱼的急性毒性测试结果可以用来快速检测饮用水和地表水源水农药污染的毒性。
