The major tectonic zone that passes through the border regions of the Anhui, Zhejiang, and Jiangxi Provinces in southeast China has been commonly referred to as the Wan-Zhe-Gan fault zone. Geologically, this zone cons...The major tectonic zone that passes through the border regions of the Anhui, Zhejiang, and Jiangxi Provinces in southeast China has been commonly referred to as the Wan-Zhe-Gan fault zone. Geologically, this zone consists of several regional fault belts of various ages and orientations. We have categorized the faults into four age groups based on field investigations. The Neoproterozoic faults are northeast striking. They start from the northeast Jiangxi Province and extend northeastward to Fuchuan in Anhui Province, the same location of the northeast Jiangxi-Fuchuan ophiolite belt. The faults probably acted during the Neoproterozoic as a boundary fault zone of a plate or a block suture with melange along the faults. The nearly east-west- or east-northeast-striking faults are of Silurian ages (40Ar/39Ar age 429 Ma). This group includes the Qimen-Shexian fault and the Jiangwang-Jiekou ductile shear belt. They represent a major tectonic boundary in the basement because the two sides of the fault have clear dissimilarities. The third group of faults is north-northeast striking, having formed since the early-middle Triassic with 40Ar/39Ar ages of 230-254 Ma. They form a fault belt starting from Yiyang in northern Jiangxi and connect with the Wucheng as well as the Ningguo-Jixi faults. This fault belt is a key fault-magmatic belt controlling the formation of Jurassic-Cretaceous red basins, ore distribution, magmatic activity, and mineralization. When it reactivated during the late Cretaceous, the belt behaved as a series of reverse faults from southeast to northwest and composed the fourth fault group. Therefore, classifying the Wan-Zhe-Gan fault zone into four fault groups will help in the analysis of the tectonic evolution of the eastern segment of the Jiangnan orogen since the Neoproterozoic era.展开更多
This novel rice line is developed recently’by a research team led by Associate Prof QIU Baiqin, Crop Breeding and Cultivation Institute, Zhejiang Academy of Agricultural Sciences. Zhe 9248 is of high resistance to bl...This novel rice line is developed recently’by a research team led by Associate Prof QIU Baiqin, Crop Breeding and Cultivation Institute, Zhejiang Academy of Agricultural Sciences. Zhe 9248 is of high resistance to blast, early mature and of higher yield. Being soft, smooth, and glossy, the cooked rice of "Zhe 9248" has excellent eating quality. The kernels of "Zhe 9248" are hardened to such a degree that they could be capa-展开更多
Phytoplankton productivity and community structure in marginal seas have been altered significantly during the past three decades, but it is still a challenge to distinguish the forcing mechanisms between climate chan...Phytoplankton productivity and community structure in marginal seas have been altered significantly during the past three decades, but it is still a challenge to distinguish the forcing mechanisms between climate change and anthropogenic activities. High time-resolution biomarker records of two 210Pb-dated sediment cores(#34: 28.5°N, 122.272°E; CJ12-1269: 28.861 9°N, 122.515 3°E) from the Min-Zhe coastal mud area were compared to reveal changes of phytoplankton productivity and community structure over the past 100 years. Phytoplankton productivity started to increase gradually from the 1970 s and increased rapidly after the late 1990 s at Site #34; and it started to increase gradually from the middle 1960 s and increased rapidly after the late 1980 s at Site CJ12-1269. Productivity of Core CJ12-1269 was higher than that of Core #34. Phytoplankton community structure variations displayed opposite patterns in the two cores. The decreasing D/B(dinosterol/brassicasterol) ratio of Core #34 since the 1960 s revealed increased diatom contribution to total productivity. In contrast, the increasing D/B ratio of Core CJ12-1269 since the 1950 s indicated increased dinoflagellate contribution to total productivity. Both the productivity increase and the increased dinoflagellate contribution in Core CJ12-1269 since the 1950–1960s were mainly caused by anthropogenic activities, as the location was closer to the Changjiang River Estuary with higher nutrient concentration and decreasing Si/N ratios. However, increased diatom contribution in Core #34 is proposed to be caused by increased coastal upwelling, with higher nutrient concentration and higher Si/N ratios.展开更多
Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescrip...Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups,i.e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule , serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation,while all the plasma group presented platelet aggregation. P1, P5, Pmax, t and TM have no significant difference (P>0. 05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin. P1, P5, t and Pmax have significant differences (P<0. 01) and TM decreased significantly (P<0. 05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P1, t and Pmax have significant difference (P<0.05), and P5 and TM are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P1, P5, t and Pmax have significant differences (P<0. 01), P1, TM have also significant(P<0. 05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P1 ,P5 and Pmax have significant differences (P<0.05), and t and Pmax are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.展开更多
Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(...Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(EZD),which has shown good curative effects in clinical practice.However,the pharmacodynamic material basis and mechanism have not yet been revealed.A strategy integrating lipidomics,network pharmacology and pharmacokinetics was used to reveal the active components and mechanisms of EZD against NAFLD.The histopathological results showed that EZD attenuated the degrees of collagen deposition and steatosis in the livers of nonalcoholic steatofibrosis model mice.Furthermore,glycerophospholipid metabolism,arachidonic acid metabolism,glycerolipid metabolism and linoleic acid metabolism with phospholipase A2 group IVA(PLA2G4A)and cytochrome P450 as the core targets and 12,13-cis-epoxyoctadecenoic acid,12(S)-hydroxyeicosatetraenoic acid,leukotriene B4,prostaglandin E2,phosphatidylcholines(PCs)and triacylglycerols(TGs)as the main lipids were found to be involved in the treatment of NAFLD by EZD.Importantly,naringenin,artemetin,canadine,and bicuculline were identified as the active ingredients of EZD against NAFLD;in particular,naringenin reduces PC consumption by inhibiting the expression of PLA2G4A and thus promotes sufficient synthesis of very-low-density lipoprotein to transport excess TGs in the liver.This research provides valuable data and theoretical support for the application of EZD against NAFLD.展开更多
The global carbon cycle has played a key role in mitigating global warming and climate change.Long-term natural and anthropogenic processes influence the composition,sources,burial rates,and fluxes of carbon in sedime...The global carbon cycle has played a key role in mitigating global warming and climate change.Long-term natural and anthropogenic processes influence the composition,sources,burial rates,and fluxes of carbon in sediments on the continental shelf of China.In this study,the rates,fluxes,and amounts of carbon storage at the centennial scale were estimated and demonstrated using the case study of three fine-grained sediment cores from the central South Yellow Sea area(SYSA) and Min-Zhe belt(MZB),East China Sea.Based on the high-resolution temporal sequences of total carbon(TC)and total organic carbon(TOC)contents,we reconstructed the annual variations of historical marine carbon storage,and explored the influence of terrestrial and marine sources on carbon burial at the centennial scale.The estimated TC storage over 100 years was 1.18×10~8 t in the SYSA and 1.45×10~9 t in the MZB.The corrected TOC storage fluxes at the centennial scale ranged from 17 to 28 t/(km^2·a)in the SYSA and from 56 to 148 t/(km^2·a)in the MZB.The decrease of terrestrial materials and the increase of marine primary production suggest that the TOC buried in the sediments in the SYSA and MZB was mainly derived from the marine autogenetic source.In the MZB,two depletion events occurred in TC and TOC storage from 1985 to 1987 and 2003 to 2006,which were coeval with the water impoundment in the Gezhouba and Three Gorges dams,respectively.The high-resolution records of the carbon storage rates and fluxes in the SYSA and MZB reflect the synchronous responses to human activities and provide an important reference for assessing the carbon sequestration capacity of the marginal seas of China.展开更多
Zhejiang is a coastal province with advanced economy,solid foundation of industry and agriculture and all-comprehensive industry sectors,and with lignht industry andtextile as the mainstay Its popularized education,ad...Zhejiang is a coastal province with advanced economy,solid foundation of industry and agriculture and all-comprehensive industry sectors,and with lignht industry andtextile as the mainstay Its popularized education,advancedscience and technology and ample supply of high-quality labourforce benefit itselt involving in foreign project contracting,labour export and overseas investment.Since 1979,Zhejianghas accepted more than ten foreign projects worth US$300million and supplied labour service to 40 countries。展开更多
The difference analysis of physical-mechanical properties of muddy sediments is made in the central South Yellow Sea and the Zhe-Min(Zhejiang Province to Fujian Province of China) coastal area. The results show that...The difference analysis of physical-mechanical properties of muddy sediments is made in the central South Yellow Sea and the Zhe-Min(Zhejiang Province to Fujian Province of China) coastal area. The results show that sediments in the two regions are both dominated by mud. There are perfect negative power function correlations between the water content and the density, the compression coefficient and the compression modulus; a good positive power function correlation between the liquid limit and the plastic limit, a perfect positive linear correlation between the water content and the void ratio, and a perfect polynomial function correlation between the miniature vane shear strength and the pocket penetration resistance. In general, compared with sediments in the Zhe-Min coastal area, sediments in the central South Yellow Sea possess high water content, high void ratio,low density, high plasticity, high compressibility, low shear strength. The causes of the differences between physical-mechanical properties of sediments are analyzed from the topographic features, material sources,hydrodynamic conditions, deposition rate, and material composition. Compared with the Zhe-Min coastal area,the central South Yellow Sea is far from the Mainland and low-lying; has poor hydrodynamic condition; the materials diffused to the area are less and dominated by fine clay, have the high content of smectite and organic matters. These factors lead to sediments of the central South Yellow Sea has the higher water content, the higher plasticity, the lower density, and the lower strength than sediments in the Zhe-Min coastal area.展开更多
The lethal and sublethal effects of oils on aquatic organisms have been widely investigated, but the potential molecular impacts of oils on aquatic organisms are remaining unclear now. In order to realize the effects ...The lethal and sublethal effects of oils on aquatic organisms have been widely investigated, but the potential molecular impacts of oils on aquatic organisms are remaining unclear now. In order to realize the effects of diesel oil on the Zhe oyster, the RAPD (random amplified polymorphic DNA) technique was used. RAPD is a useful assay procedure for the detection of genotoxin-induced DNA damage and mutations. In the present study, the Zhe oysters were exposed to diesel oil at different concentrations and for different exposure periods. The results showed that the DNA band change in RAPD profiles of oysters following diesel oil treatment included loss of normal DNA bands, the appearance of new DNA bands and variations in DNA intensity compared to oysters not exposed to diesel oil. The effects of changes to GTS (genome template stability) were time- and concentration-dependent, the GTS of 10 mg/L was 82.46%, 80.70% and 63.15% in the 8, 16 and 32 days, the GTS of 20 mg/L was 75.44%, 71.93% and 56.14% in the 8, 16 and 32 days, the GTS of 40 mg/L was 73.68%, 70.18% and 56.14% in the 8, 16 and 32 days, respectively. The DNA polymorphisms detected by RAPD analysis could be used as a useful biomarker assay for the detection of genotoxic effects in diesel oil pollution on the oysters, and may be useful for environmental contamination risk assessment.展开更多
基金the Open Research Program of the State Key Laboratory of Geological Processes and Mineral Resources,China University of Geosciences(Grant No GPMR 200836)the National Natural Science Foundation of China(Grant No90814006+1 种基金40772134) for financial supportthe project "Research on the W-Mo Polymetallic Regularity in Dongyuan,Qimen and Xiaoyao,Jixi(Grant No2009-20)"
文摘The major tectonic zone that passes through the border regions of the Anhui, Zhejiang, and Jiangxi Provinces in southeast China has been commonly referred to as the Wan-Zhe-Gan fault zone. Geologically, this zone consists of several regional fault belts of various ages and orientations. We have categorized the faults into four age groups based on field investigations. The Neoproterozoic faults are northeast striking. They start from the northeast Jiangxi Province and extend northeastward to Fuchuan in Anhui Province, the same location of the northeast Jiangxi-Fuchuan ophiolite belt. The faults probably acted during the Neoproterozoic as a boundary fault zone of a plate or a block suture with melange along the faults. The nearly east-west- or east-northeast-striking faults are of Silurian ages (40Ar/39Ar age 429 Ma). This group includes the Qimen-Shexian fault and the Jiangwang-Jiekou ductile shear belt. They represent a major tectonic boundary in the basement because the two sides of the fault have clear dissimilarities. The third group of faults is north-northeast striking, having formed since the early-middle Triassic with 40Ar/39Ar ages of 230-254 Ma. They form a fault belt starting from Yiyang in northern Jiangxi and connect with the Wucheng as well as the Ningguo-Jixi faults. This fault belt is a key fault-magmatic belt controlling the formation of Jurassic-Cretaceous red basins, ore distribution, magmatic activity, and mineralization. When it reactivated during the late Cretaceous, the belt behaved as a series of reverse faults from southeast to northwest and composed the fourth fault group. Therefore, classifying the Wan-Zhe-Gan fault zone into four fault groups will help in the analysis of the tectonic evolution of the eastern segment of the Jiangnan orogen since the Neoproterozoic era.
文摘This novel rice line is developed recently’by a research team led by Associate Prof QIU Baiqin, Crop Breeding and Cultivation Institute, Zhejiang Academy of Agricultural Sciences. Zhe 9248 is of high resistance to blast, early mature and of higher yield. Being soft, smooth, and glossy, the cooked rice of "Zhe 9248" has excellent eating quality. The kernels of "Zhe 9248" are hardened to such a degree that they could be capa-
基金The National Basic Research Program of China(973 Program)under contract No.2010CB428901the National Natural Science Foundation of China under contract Nos 41020164005,40976042 and 41076036
文摘Phytoplankton productivity and community structure in marginal seas have been altered significantly during the past three decades, but it is still a challenge to distinguish the forcing mechanisms between climate change and anthropogenic activities. High time-resolution biomarker records of two 210Pb-dated sediment cores(#34: 28.5°N, 122.272°E; CJ12-1269: 28.861 9°N, 122.515 3°E) from the Min-Zhe coastal mud area were compared to reveal changes of phytoplankton productivity and community structure over the past 100 years. Phytoplankton productivity started to increase gradually from the 1970 s and increased rapidly after the late 1990 s at Site #34; and it started to increase gradually from the middle 1960 s and increased rapidly after the late 1980 s at Site CJ12-1269. Productivity of Core CJ12-1269 was higher than that of Core #34. Phytoplankton community structure variations displayed opposite patterns in the two cores. The decreasing D/B(dinosterol/brassicasterol) ratio of Core #34 since the 1960 s revealed increased diatom contribution to total productivity. In contrast, the increasing D/B ratio of Core CJ12-1269 since the 1950 s indicated increased dinoflagellate contribution to total productivity. Both the productivity increase and the increased dinoflagellate contribution in Core CJ12-1269 since the 1950–1960s were mainly caused by anthropogenic activities, as the location was closer to the Changjiang River Estuary with higher nutrient concentration and decreasing Si/N ratios. However, increased diatom contribution in Core #34 is proposed to be caused by increased coastal upwelling, with higher nutrient concentration and higher Si/N ratios.
文摘Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups,i.e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule , serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation,while all the plasma group presented platelet aggregation. P1, P5, Pmax, t and TM have no significant difference (P>0. 05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin. P1, P5, t and Pmax have significant differences (P<0. 01) and TM decreased significantly (P<0. 05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P1, t and Pmax have significant difference (P<0.05), and P5 and TM are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P1, P5, t and Pmax have significant differences (P<0. 01), P1, TM have also significant(P<0. 05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P1 ,P5 and Pmax have significant differences (P<0.05), and t and Pmax are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.
基金supported by Ningbo Natural Science Foundation(Grant No.:2022J229)Project of Ningbo Leading Medical&Health Discipline(Project No.:2022-S04)+1 种基金Opened-end Fund of Key Laboratory(Grant No.:KFJJ-202101)Graduate Student Scientific Research and Innovation Fund of Ningbo University(Grant No.:IF2022193)。
文摘Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(EZD),which has shown good curative effects in clinical practice.However,the pharmacodynamic material basis and mechanism have not yet been revealed.A strategy integrating lipidomics,network pharmacology and pharmacokinetics was used to reveal the active components and mechanisms of EZD against NAFLD.The histopathological results showed that EZD attenuated the degrees of collagen deposition and steatosis in the livers of nonalcoholic steatofibrosis model mice.Furthermore,glycerophospholipid metabolism,arachidonic acid metabolism,glycerolipid metabolism and linoleic acid metabolism with phospholipase A2 group IVA(PLA2G4A)and cytochrome P450 as the core targets and 12,13-cis-epoxyoctadecenoic acid,12(S)-hydroxyeicosatetraenoic acid,leukotriene B4,prostaglandin E2,phosphatidylcholines(PCs)and triacylglycerols(TGs)as the main lipids were found to be involved in the treatment of NAFLD by EZD.Importantly,naringenin,artemetin,canadine,and bicuculline were identified as the active ingredients of EZD against NAFLD;in particular,naringenin reduces PC consumption by inhibiting the expression of PLA2G4A and thus promotes sufficient synthesis of very-low-density lipoprotein to transport excess TGs in the liver.This research provides valuable data and theoretical support for the application of EZD against NAFLD.
基金Supported by the National Basic Research Program of China(973 Program)(No.2012CB956004)the Fundamental Research Funds for the Central Universities(No.16lgjc22)
文摘The global carbon cycle has played a key role in mitigating global warming and climate change.Long-term natural and anthropogenic processes influence the composition,sources,burial rates,and fluxes of carbon in sediments on the continental shelf of China.In this study,the rates,fluxes,and amounts of carbon storage at the centennial scale were estimated and demonstrated using the case study of three fine-grained sediment cores from the central South Yellow Sea area(SYSA) and Min-Zhe belt(MZB),East China Sea.Based on the high-resolution temporal sequences of total carbon(TC)and total organic carbon(TOC)contents,we reconstructed the annual variations of historical marine carbon storage,and explored the influence of terrestrial and marine sources on carbon burial at the centennial scale.The estimated TC storage over 100 years was 1.18×10~8 t in the SYSA and 1.45×10~9 t in the MZB.The corrected TOC storage fluxes at the centennial scale ranged from 17 to 28 t/(km^2·a)in the SYSA and from 56 to 148 t/(km^2·a)in the MZB.The decrease of terrestrial materials and the increase of marine primary production suggest that the TOC buried in the sediments in the SYSA and MZB was mainly derived from the marine autogenetic source.In the MZB,two depletion events occurred in TC and TOC storage from 1985 to 1987 and 2003 to 2006,which were coeval with the water impoundment in the Gezhouba and Three Gorges dams,respectively.The high-resolution records of the carbon storage rates and fluxes in the SYSA and MZB reflect the synchronous responses to human activities and provide an important reference for assessing the carbon sequestration capacity of the marginal seas of China.
文摘Zhejiang is a coastal province with advanced economy,solid foundation of industry and agriculture and all-comprehensive industry sectors,and with lignht industry andtextile as the mainstay Its popularized education,advancedscience and technology and ample supply of high-quality labourforce benefit itselt involving in foreign project contracting,labour export and overseas investment.Since 1979,Zhejianghas accepted more than ten foreign projects worth US$300million and supplied labour service to 40 countries。
基金The NSFC-Shandong Joint Fund for Marine Science Research Centers under contract No.U150640007the Natural Science Foundation of Shandong Province of China under contract No.BS2015HZ009the National Natural Science Foundation of China under contract No.41506071
文摘The difference analysis of physical-mechanical properties of muddy sediments is made in the central South Yellow Sea and the Zhe-Min(Zhejiang Province to Fujian Province of China) coastal area. The results show that sediments in the two regions are both dominated by mud. There are perfect negative power function correlations between the water content and the density, the compression coefficient and the compression modulus; a good positive power function correlation between the liquid limit and the plastic limit, a perfect positive linear correlation between the water content and the void ratio, and a perfect polynomial function correlation between the miniature vane shear strength and the pocket penetration resistance. In general, compared with sediments in the Zhe-Min coastal area, sediments in the central South Yellow Sea possess high water content, high void ratio,low density, high plasticity, high compressibility, low shear strength. The causes of the differences between physical-mechanical properties of sediments are analyzed from the topographic features, material sources,hydrodynamic conditions, deposition rate, and material composition. Compared with the Zhe-Min coastal area,the central South Yellow Sea is far from the Mainland and low-lying; has poor hydrodynamic condition; the materials diffused to the area are less and dominated by fine clay, have the high content of smectite and organic matters. These factors lead to sediments of the central South Yellow Sea has the higher water content, the higher plasticity, the lower density, and the lower strength than sediments in the Zhe-Min coastal area.
文摘The lethal and sublethal effects of oils on aquatic organisms have been widely investigated, but the potential molecular impacts of oils on aquatic organisms are remaining unclear now. In order to realize the effects of diesel oil on the Zhe oyster, the RAPD (random amplified polymorphic DNA) technique was used. RAPD is a useful assay procedure for the detection of genotoxin-induced DNA damage and mutations. In the present study, the Zhe oysters were exposed to diesel oil at different concentrations and for different exposure periods. The results showed that the DNA band change in RAPD profiles of oysters following diesel oil treatment included loss of normal DNA bands, the appearance of new DNA bands and variations in DNA intensity compared to oysters not exposed to diesel oil. The effects of changes to GTS (genome template stability) were time- and concentration-dependent, the GTS of 10 mg/L was 82.46%, 80.70% and 63.15% in the 8, 16 and 32 days, the GTS of 20 mg/L was 75.44%, 71.93% and 56.14% in the 8, 16 and 32 days, the GTS of 40 mg/L was 73.68%, 70.18% and 56.14% in the 8, 16 and 32 days, respectively. The DNA polymorphisms detected by RAPD analysis could be used as a useful biomarker assay for the detection of genotoxic effects in diesel oil pollution on the oysters, and may be useful for environmental contamination risk assessment.