Background:To explore the mechanism of action of Zhishi Daozhi decoction in treating diarrhea using network pharmacology and molecular docking.Methods:The pharmacological components and targets of Zhishi Daozhi decoct...Background:To explore the mechanism of action of Zhishi Daozhi decoction in treating diarrhea using network pharmacology and molecular docking.Methods:The pharmacological components and targets of Zhishi Daozhi decoction were searched by The TCMSP and Uniprot database and the disease genes of diarrhea were obtained from GeneCard and diagenetic databases.The network software venny 2.1.0 was used to screen the intersection targets of Zhishi Daozhi decoction in treating diarrhea.STRING database was used to construct protein interaction network,Metascape database analysis platform for gene ontology enrichment and Kyoto Gene and Genome Encyclopedia pathway enrichment analysis.Using Cytoscape 3.9.1 software to map the“Chinese herbal medicine-active ingredient-target”network and perform network topology to identify the core genes.Selecting core genes and critical components for molecular docking.Results:203 active ingredients and 259 target genes were obtained from Zhishi Daozhi decoction,206 genes were cross-linked with the disease gene control after de-duplication,1001 entries meeting the screening criteria were obtained after gene ontology enrichment analysis,and 169 entries were obtained after Kyoto Gene and Genome Encyclopedia enrichment analysis,in which the biological processes were mainly enriched in the cellular responses to lipids,hormones,radiation,growth factors,and cell fractionation.The Kyoto Gene and Genome Encyclopedia pathway enrichment analysis suggested that it mainly covers the cancer pathway,hepatitis B,cellular senescence,IL-17 signaling pathway,PI3K-Akt signaling pathway,etc.The molecular docking results showed that the vital active ingredients,such as lignocaine and quercetin,with better binding activity to the vital core targets,such as MMP3,AKT1,and CCL2.Conclusion:The active ingredients such as quercetin and luteolin in Zhishi Daozhi decoction may act through signaling pathways such as IL-17 signaling pathway,PI3K-Akt signaling pathway,TGF-βsignaling pathway,and other pathways,and act on the critical core targets such as MMP3,AKT1,HSP90AA1,FOS,CCL2,and TGFB1 to enhance intestinal barrier function,reduce the inflammatory response and regulate intestinal flora,thus achieving the purpose of treating diarrhea.展开更多
AIM To compare the therapeutic effect of the herbal medicine Zhishi Xiaopiwan with that of Cisapride in the treatment of functional dyspepsia (FD). METHODS Fifty one FD patients were randomized into Herbal...AIM To compare the therapeutic effect of the herbal medicine Zhishi Xiaopiwan with that of Cisapride in the treatment of functional dyspepsia (FD). METHODS Fifty one FD patients were randomized into Herbal group ( n =27) and Cisapride group ( n =24). Two groups were given a four week treatment of Zhishi Xiaopiwan 100ml , tid, a.c. and Cisapride 5mg , tid, a.c. respectively. Patients′ symptoms were assessed and 39 patients′ (22 of Herbal group and 17 of Cisapride group) gastric liquid emptying times were measured with ultrasonography before and after the treatment. RESULTS The therapeutic effective rates of Herbal group and Cisapride group were 81 49% and 87 50% ( P >0 05). The half gastric emptying time (GET 50 ) and gastric emptying time (GET) of healthy controls and FD patients were 36 12min±10 22min vs 52 95min±13 49min and 87 07min±21 11min vs 120 74min±23 08min ( P <0 001). The GET 50 and GET of Herbal group before and after the treatment were 51 63min±13 15min vs 45 62min±10 82min and 117 34min±23 29min vs 103 26min±22 19min ( P <0 01). The results of Cisapride group were 54 66min±14 14min vs 40 95min±11 29min and 125 12min±24 47min vs 95 49min±22 31min ( P <0 01). The differences in values (median) of GET 50 and GET for Herbal group and Cisapride group before and after treatment were 5 75min vs 17 18min and 13 22min vs 33 54min ( P <0 05). CONCLUSION Delayed gastric emptying is one of the pathogenesis of FD. Both Zhishi Xiaopi pills and Cisapride can effectively alleviate the symptoms of FD and accelerate gastric liquid emptying. The effect of Zhishi Xiaopiwan on enhancing gastric motility is comparable with but less than that of Cisapride .展开更多
目的分析加味枳实薤白桂枝汤对肥胖症(脾虚湿阻型)合并2型糖尿病患者糖脂代谢指标的影响。方法选择160例肥胖症(脾虚湿阻型)同时合并2型糖尿病的患者,随机分为试验组(81例)与对照组(79例)。对照组予以规范生活指导及规范的降糖药物干预...目的分析加味枳实薤白桂枝汤对肥胖症(脾虚湿阻型)合并2型糖尿病患者糖脂代谢指标的影响。方法选择160例肥胖症(脾虚湿阻型)同时合并2型糖尿病的患者,随机分为试验组(81例)与对照组(79例)。对照组予以规范生活指导及规范的降糖药物干预,试验组在对照组治疗基础上给予加味枳实薤白桂枝汤口服,疗程共2个月,比较两组治疗前后中医证候积分、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、三酰甘油(TG)、空腹血糖(FPG)、餐后2 h血糖(2 h FPG)、糖化血红蛋白(HbA1c)、空腹胰岛素(FINS)、尿酸(UA)、体重指数(BMI)、同型半胱氨酸(Hcy)水平。结果治疗后,试验组中医证候积分、BMI、UA、Hcy、TG、LDL-C、FPG、HbA1c、2 h FPG、FINS水平显著低于对照组(P<0.05)。结论加味枳实薤白桂枝汤可以调节肥胖症合并2型糖尿病该类型患者的糖脂代谢指标。展开更多
基金supported by the science and technology innovation particular topic of Maoming City(No.2021S0047)science and technology innovation particular topic of Maoming City(No.2022S0014).
文摘Background:To explore the mechanism of action of Zhishi Daozhi decoction in treating diarrhea using network pharmacology and molecular docking.Methods:The pharmacological components and targets of Zhishi Daozhi decoction were searched by The TCMSP and Uniprot database and the disease genes of diarrhea were obtained from GeneCard and diagenetic databases.The network software venny 2.1.0 was used to screen the intersection targets of Zhishi Daozhi decoction in treating diarrhea.STRING database was used to construct protein interaction network,Metascape database analysis platform for gene ontology enrichment and Kyoto Gene and Genome Encyclopedia pathway enrichment analysis.Using Cytoscape 3.9.1 software to map the“Chinese herbal medicine-active ingredient-target”network and perform network topology to identify the core genes.Selecting core genes and critical components for molecular docking.Results:203 active ingredients and 259 target genes were obtained from Zhishi Daozhi decoction,206 genes were cross-linked with the disease gene control after de-duplication,1001 entries meeting the screening criteria were obtained after gene ontology enrichment analysis,and 169 entries were obtained after Kyoto Gene and Genome Encyclopedia enrichment analysis,in which the biological processes were mainly enriched in the cellular responses to lipids,hormones,radiation,growth factors,and cell fractionation.The Kyoto Gene and Genome Encyclopedia pathway enrichment analysis suggested that it mainly covers the cancer pathway,hepatitis B,cellular senescence,IL-17 signaling pathway,PI3K-Akt signaling pathway,etc.The molecular docking results showed that the vital active ingredients,such as lignocaine and quercetin,with better binding activity to the vital core targets,such as MMP3,AKT1,and CCL2.Conclusion:The active ingredients such as quercetin and luteolin in Zhishi Daozhi decoction may act through signaling pathways such as IL-17 signaling pathway,PI3K-Akt signaling pathway,TGF-βsignaling pathway,and other pathways,and act on the critical core targets such as MMP3,AKT1,HSP90AA1,FOS,CCL2,and TGFB1 to enhance intestinal barrier function,reduce the inflammatory response and regulate intestinal flora,thus achieving the purpose of treating diarrhea.
文摘AIM To compare the therapeutic effect of the herbal medicine Zhishi Xiaopiwan with that of Cisapride in the treatment of functional dyspepsia (FD). METHODS Fifty one FD patients were randomized into Herbal group ( n =27) and Cisapride group ( n =24). Two groups were given a four week treatment of Zhishi Xiaopiwan 100ml , tid, a.c. and Cisapride 5mg , tid, a.c. respectively. Patients′ symptoms were assessed and 39 patients′ (22 of Herbal group and 17 of Cisapride group) gastric liquid emptying times were measured with ultrasonography before and after the treatment. RESULTS The therapeutic effective rates of Herbal group and Cisapride group were 81 49% and 87 50% ( P >0 05). The half gastric emptying time (GET 50 ) and gastric emptying time (GET) of healthy controls and FD patients were 36 12min±10 22min vs 52 95min±13 49min and 87 07min±21 11min vs 120 74min±23 08min ( P <0 001). The GET 50 and GET of Herbal group before and after the treatment were 51 63min±13 15min vs 45 62min±10 82min and 117 34min±23 29min vs 103 26min±22 19min ( P <0 01). The results of Cisapride group were 54 66min±14 14min vs 40 95min±11 29min and 125 12min±24 47min vs 95 49min±22 31min ( P <0 01). The differences in values (median) of GET 50 and GET for Herbal group and Cisapride group before and after treatment were 5 75min vs 17 18min and 13 22min vs 33 54min ( P <0 05). CONCLUSION Delayed gastric emptying is one of the pathogenesis of FD. Both Zhishi Xiaopi pills and Cisapride can effectively alleviate the symptoms of FD and accelerate gastric liquid emptying. The effect of Zhishi Xiaopiwan on enhancing gastric motility is comparable with but less than that of Cisapride .
文摘目的分析加味枳实薤白桂枝汤对肥胖症(脾虚湿阻型)合并2型糖尿病患者糖脂代谢指标的影响。方法选择160例肥胖症(脾虚湿阻型)同时合并2型糖尿病的患者,随机分为试验组(81例)与对照组(79例)。对照组予以规范生活指导及规范的降糖药物干预,试验组在对照组治疗基础上给予加味枳实薤白桂枝汤口服,疗程共2个月,比较两组治疗前后中医证候积分、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、三酰甘油(TG)、空腹血糖(FPG)、餐后2 h血糖(2 h FPG)、糖化血红蛋白(HbA1c)、空腹胰岛素(FINS)、尿酸(UA)、体重指数(BMI)、同型半胱氨酸(Hcy)水平。结果治疗后,试验组中医证候积分、BMI、UA、Hcy、TG、LDL-C、FPG、HbA1c、2 h FPG、FINS水平显著低于对照组(P<0.05)。结论加味枳实薤白桂枝汤可以调节肥胖症合并2型糖尿病该类型患者的糖脂代谢指标。
