Summary： Zinc finger of the cerebellum （ZIC 1）, one of ZIC family genes, has been shown to play im- portant roles in many cancers such as gastric cancer and breast cancer. However, there is little known about the e...Summary： Zinc finger of the cerebellum （ZIC 1）, one of ZIC family genes, has been shown to play im- portant roles in many cancers such as gastric cancer and breast cancer. However, there is little known about the expression and significance of ZIC1 in endometrial cancer. The aim of this study was to de- termine the expression pattern and clinicopathological significance of ZIC1 in endometrial cancer. The rnRNA and protein expression of ZIC1 in endometrial cancer tissues was detected using the reverse- transcription polymerase chain reaction and Western blotting, respectively. Immunostaining of ZIC1 in 99 endometrial cancer samples was examined and its associations with clinicopathological parameters were analyzed. Hec-l-B cells were transfected with Z1CI-shRNA or sc-shRNA, and cell proliferation was assayed. Hec-l-B cells stably transfected with ZICI-shRNA or sc-shRNA were subcutaneously in- oculated into nude mice, and the tumor weight was measured. A significantly increased expression of ZIC1 mRNA and protein was observed in endometrial cancer tissues compared to that in normal endo- metrial tissues （P〈0.05）. Immunohistochemical analysis showed that strong cytoplasmic immunostain- ing of ZIC1 was observed in almost all endometrial cancer samples （90/99） while light and moderate immunostaining of ZIC 1 was only detected in 17 of 30 （56.7%） normal tissues. Moreover, up-regulation of ZIC1 was significantly correlated with age, disease stage, TNM stage and FIGO stage （P〈0.05）. The down-regulated expression of ZIC1 contributed to the inhibition of cell proliferation, and inhibited the growth of tumor. It was concluded that ZIC1 is over-expressed in endometrial cancer tissue but not in normal tissue, and positively correlated to the malignant biological behavior of endometrial carcino- genesis.展开更多
文摘Summary： Zinc finger of the cerebellum （ZIC 1）, one of ZIC family genes, has been shown to play im- portant roles in many cancers such as gastric cancer and breast cancer. However, there is little known about the expression and significance of ZIC1 in endometrial cancer. The aim of this study was to de- termine the expression pattern and clinicopathological significance of ZIC1 in endometrial cancer. The rnRNA and protein expression of ZIC1 in endometrial cancer tissues was detected using the reverse- transcription polymerase chain reaction and Western blotting, respectively. Immunostaining of ZIC1 in 99 endometrial cancer samples was examined and its associations with clinicopathological parameters were analyzed. Hec-l-B cells were transfected with Z1CI-shRNA or sc-shRNA, and cell proliferation was assayed. Hec-l-B cells stably transfected with ZICI-shRNA or sc-shRNA were subcutaneously in- oculated into nude mice, and the tumor weight was measured. A significantly increased expression of ZIC1 mRNA and protein was observed in endometrial cancer tissues compared to that in normal endo- metrial tissues （P〈0.05）. Immunohistochemical analysis showed that strong cytoplasmic immunostain- ing of ZIC1 was observed in almost all endometrial cancer samples （90/99） while light and moderate immunostaining of ZIC 1 was only detected in 17 of 30 （56.7%） normal tissues. Moreover, up-regulation of ZIC1 was significantly correlated with age, disease stage, TNM stage and FIGO stage （P〈0.05）. The down-regulated expression of ZIC1 contributed to the inhibition of cell proliferation, and inhibited the growth of tumor. It was concluded that ZIC1 is over-expressed in endometrial cancer tissue but not in normal tissue, and positively correlated to the malignant biological behavior of endometrial carcino- genesis.
