[Objectives] To explore the clinical effect of Modified Gegen Qinlian Decoction combined with western medicine on children with rotavirus enteritis with damp-heat syndrome and its influence on myocardial enzymes.
Knowledge of genetic diversity is essential for any future varietal improvement program. Thus, the objective of this study is to contribute to a better knowledge of the genetic diversity within 15 sesame accessions, u...Knowledge of genetic diversity is essential for any future varietal improvement program. Thus, the objective of this study is to contribute to a better knowledge of the genetic diversity within 15 sesame accessions, using biochemical markers and RAPD. The accessions are of various sources and seeds of different colors;color being an important feature in the sesame market. SDS-PAGE on protein extracts of sesame seeds revealed 31 protein markers, only 4 of which were polymorphic, indicating that this technique is not suitable for studying genetic diversity in S. indicum. The analyses on isoenzymatic extracts of esterase from sesame seedlings made it possible, to distinguish and classify the accessions into 2 major groups. RAPD analysis of genomic DNA using 10-mer primers revealed a high inter-accession genetic diversity. A total of 53 DNA markers, including 48 polymorphic, were produced. The coefficient of similarity between accessions ranged from 0.343 to 0.897. RAPD markers were found to be more suitable for studying genetic diversity within sesame germplasm than isozyme analyzes. Nevertheless, geographical distributions and seed colors are not related to the classes formed.展开更多
Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of th...Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining展开更多
The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that...The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that the maize hybrid impurity is mainly composed of three parts:mixed with the seeds from parents or other source, contaminated with the seeds resulting from unknown pollination and variable in genetic background. It was found that the varietal purity is higher than the zymogram purity and they showed a linear relationship. The regression coefficient (r) between varietal purity and zymogram purity is 0.898 2. There is an average of 3.5% variable in the field. However,using a known rate of the mixed seeds, their regression coefficient is 0.999 9. It was also found that the examinational mix rate is 0.33% higher than the known mix rate. The seedling rate of the single cross hybrids is 85.71%,but that of hybrids mixed with their parental lines is only 77%. Therefore,it is reasonable to claim that zymogram purity is lower than the varietal purity.展开更多
文摘[Objectives] To explore the clinical effect of Modified Gegen Qinlian Decoction combined with western medicine on children with rotavirus enteritis with damp-heat syndrome and its influence on myocardial enzymes.
文摘Knowledge of genetic diversity is essential for any future varietal improvement program. Thus, the objective of this study is to contribute to a better knowledge of the genetic diversity within 15 sesame accessions, using biochemical markers and RAPD. The accessions are of various sources and seeds of different colors;color being an important feature in the sesame market. SDS-PAGE on protein extracts of sesame seeds revealed 31 protein markers, only 4 of which were polymorphic, indicating that this technique is not suitable for studying genetic diversity in S. indicum. The analyses on isoenzymatic extracts of esterase from sesame seedlings made it possible, to distinguish and classify the accessions into 2 major groups. RAPD analysis of genomic DNA using 10-mer primers revealed a high inter-accession genetic diversity. A total of 53 DNA markers, including 48 polymorphic, were produced. The coefficient of similarity between accessions ranged from 0.343 to 0.897. RAPD markers were found to be more suitable for studying genetic diversity within sesame germplasm than isozyme analyzes. Nevertheless, geographical distributions and seed colors are not related to the classes formed.
文摘Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining
文摘The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that the maize hybrid impurity is mainly composed of three parts:mixed with the seeds from parents or other source, contaminated with the seeds resulting from unknown pollination and variable in genetic background. It was found that the varietal purity is higher than the zymogram purity and they showed a linear relationship. The regression coefficient (r) between varietal purity and zymogram purity is 0.898 2. There is an average of 3.5% variable in the field. However,using a known rate of the mixed seeds, their regression coefficient is 0.999 9. It was also found that the examinational mix rate is 0.33% higher than the known mix rate. The seedling rate of the single cross hybrids is 85.71%,but that of hybrids mixed with their parental lines is only 77%. Therefore,it is reasonable to claim that zymogram purity is lower than the varietal purity.
